saci xbai  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs saci xbai
    Saci Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/saci xbai/product/New England Biolabs
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    saci xbai - by Bioz Stars, 2020-04
    95/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: .. The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI. .. A plasmid having the SS::cutinase fusion cloned in the correct orientation behind the cauliflower mosaic virus (CaMV) 35S promoter was digested with NotI, and the 3.1-kb fragment was cloned into pART27 ( ).

    Amplification:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: The coding region of the mature cutinase of Fusarium solani f sp pisi ( ) was amplified by polymerase chain reaction (PCR) from the construct in pBluescript KS− (Stratagene, La Jolla, CA) designated pBSKS(−):LTPP:CUT (P. Kolattukudy, personal communication) by the VentR DNA Polymerase (New England Biolabs, Beverly, MA) using the oligonucleotides 5′-CUT (5′-T GCTAGC GCTGGTAGAACAACTCG-3′; NheI site underlined) and 3′-CUT (5′-TA GGTACC TCAAGCAGAACCACG-3′; KpnI site underlined). .. The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI.

    Construct:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: The coding region of the mature cutinase of Fusarium solani f sp pisi ( ) was amplified by polymerase chain reaction (PCR) from the construct in pBluescript KS− (Stratagene, La Jolla, CA) designated pBSKS(−):LTPP:CUT (P. Kolattukudy, personal communication) by the VentR DNA Polymerase (New England Biolabs, Beverly, MA) using the oligonucleotides 5′-CUT (5′-T GCTAGC GCTGGTAGAACAACTCG-3′; NheI site underlined) and 3′-CUT (5′-TA GGTACC TCAAGCAGAACCACG-3′; KpnI site underlined). .. The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI.

    Sequencing:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: .. The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI. .. A plasmid having the SS::cutinase fusion cloned in the correct orientation behind the cauliflower mosaic virus (CaMV) 35S promoter was digested with NotI, and the 3.1-kb fragment was cloned into pART27 ( ).

    Polymerase Chain Reaction:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: The coding region of the mature cutinase of Fusarium solani f sp pisi ( ) was amplified by polymerase chain reaction (PCR) from the construct in pBluescript KS− (Stratagene, La Jolla, CA) designated pBSKS(−):LTPP:CUT (P. Kolattukudy, personal communication) by the VentR DNA Polymerase (New England Biolabs, Beverly, MA) using the oligonucleotides 5′-CUT (5′-T GCTAGC GCTGGTAGAACAACTCG-3′; NheI site underlined) and 3′-CUT (5′-TA GGTACC TCAAGCAGAACCACG-3′; KpnI site underlined). .. The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI.

    Transformation Assay:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI. .. The construct called p35S::SS::Cut was transformed in the Agrobacterium strain GV3101/pMP90 ( ).

    Plasmid Preparation:

    Article Title: Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions
    Article Snippet: The 0.6-kb band was digested with NheI and KpnI and cloned behind the signal sequence from the chitinase A from tobacco ( ) by digesting the plasmid pMRE7066::SS::GFP (MRE for metallothionine-responsive element [ ]; SS for signal sequence; and GFP for green fluorescent protein) (P. Sieber and C. Nawrath, data not shown), resulting in pPMB7066:SS:CUT. .. The cohesive ends of the 0.7-kb signal sequence and the cutinase fusion obtained by digesting pPMB7066::SS::CUT with SacI-XbaI were filled in with the T4 DNA polymerase (New England Biolabs) and cloned into pART7 ( ) that had been digested with SmaI.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs saci xbai
    Saci Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/saci xbai/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    saci xbai - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    80
    New England Biolabs saci xbai digested fragment
    Saci Xbai Digested Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/saci xbai digested fragment/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    saci xbai digested fragment - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

    Image Search Results