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Millipore sabinene

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sabinene - by Bioz Stars, 2021-04

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1) Product Images from "The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases"

Article Title: The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases

Journal: Horticulture Research

doi: 10.1038/s41438-020-00412-y

Marker dependent abundance of terpenes and LOD scores for chromosome 4. a Abundance of terpene in the VOM14 F 2 biparental mapping population sorted after marker genotyping. Three hundred and twenty plants were analysed by headspace GC-MS and the terpene content was sorted by the results of the marker analysis (KASP 4-31,228,189 CAPS 4-31,218,832). Association consistencies between the abundance of β-myrcene, limonene, sabinene, α-terpinene, γ-terpinene, terpinen-4-ol, α-thujene and 4-carene, and genotype in leaf and root tissue of carrot are presented in box and whiskers (min to max, show all points) with a fold change of the abundance in log10 scale. The data were statistically analysed with Tukey’s multiple comparison test (* P

Figure Legend Snippet: Marker dependent abundance of terpenes and LOD scores for chromosome 4. a Abundance of terpene in the VOM14 F 2 biparental mapping population sorted after marker genotyping. Three hundred and twenty plants were analysed by headspace GC-MS and the terpene content was sorted by the results of the marker analysis (KASP 4-31,228,189 CAPS 4-31,218,832). Association consistencies between the abundance of β-myrcene, limonene, sabinene, α-terpinene, γ-terpinene, terpinen-4-ol, α-thujene and 4-carene, and genotype in leaf and root tissue of carrot are presented in box and whiskers (min to max, show all points) with a fold change of the abundance in log10 scale. The data were statistically analysed with Tukey’s multiple comparison test (* P

Techniques Used: Marker, Gas Chromatography-Mass Spectrometry

Characterization of a genomic region associated with sabinene production on chromosome 4. a Schematic representation of the TPS-gene cluster. Structural analysis of this region was performed based on reannotation of carrot genome assembly by GeMoMa (version 1.6.3.beta) using 12 reference genomes 22 , 28 . Receptor-like kinase (RLK) and galacturonosyltransferases (GAUT). b Amino acid sequence alignment of candidate terpene synthases from the yellow and cola mutant. Background in grey shows different residues in all five candidate terpene synthases. Differences between DcTPS04 and DcTPS54 sequences are marked in red. N-terminus of the catalytic domain (RR) and the DDIYD-conserved motif are marked in bold. The N-terminal plastid transit peptide predicted by Target P2.0 is marked with a horizontal line. The phylogram in the lower-left corner shows similarity of the catalytic domain of the 5 terpene synthases based on the translation of the nucleotide sequences from the yellow and the cola mutant. TPS-b marks the as TPS-b clade. A phylogenetic tree of all carrots TPS was previously published in ref. 23

Figure Legend Snippet: Characterization of a genomic region associated with sabinene production on chromosome 4. a Schematic representation of the TPS-gene cluster. Structural analysis of this region was performed based on reannotation of carrot genome assembly by GeMoMa (version 1.6.3.beta) using 12 reference genomes 22 , 28 . Receptor-like kinase (RLK) and galacturonosyltransferases (GAUT). b Amino acid sequence alignment of candidate terpene synthases from the yellow and cola mutant. Background in grey shows different residues in all five candidate terpene synthases. Differences between DcTPS04 and DcTPS54 sequences are marked in red. N-terminus of the catalytic domain (RR) and the DDIYD-conserved motif are marked in bold. The N-terminal plastid transit peptide predicted by Target P2.0 is marked with a horizontal line. The phylogram in the lower-left corner shows similarity of the catalytic domain of the 5 terpene synthases based on the translation of the nucleotide sequences from the yellow and the cola mutant. TPS-b marks the as TPS-b clade. A phylogenetic tree of all carrots TPS was previously published in ref. 23

Techniques Used: Sequencing, Mutagenesis

2) Product Images from "Microbial production of sabinene--a new terpene-based precursor of advanced biofuel"

Article Title: Microbial production of sabinene--a new terpene-based precursor of advanced biofuel

Journal: Microbial Cell Factories

doi: 10.1186/1475-2859-13-20

The growth of E. coli in LB medium with different concentration of commercial sabinene. The growth of the bacterial culture was determined by measuring the OD 600 (the optical density at 600 nm) with a spectrophotometer (Cary 50 UV-Vis, Varian) at 4 h, 7 h, 11 h, 14 h, 17 h, 20 h, 24 h and 36 h. The concentrations of sabinene were added to the LB medium as follows: 0 g/L (■), 0.5 g/L (●), 1 g/L (▲), 2.5 g/L (▼), 5 g/L (◄).

Figure Legend Snippet: The growth of E. coli in LB medium with different concentration of commercial sabinene. The growth of the bacterial culture was determined by measuring the OD 600 (the optical density at 600 nm) with a spectrophotometer (Cary 50 UV-Vis, Varian) at 4 h, 7 h, 11 h, 14 h, 17 h, 20 h, 24 h and 36 h. The concentrations of sabinene were added to the LB medium as follows: 0 g/L (■), 0.5 g/L (●), 1 g/L (▲), 2.5 g/L (▼), 5 g/L (◄).

Techniques Used: Concentration Assay, Spectrophotometry

GC-MS analysis of sabinene from the headspace of the sealed cultures of strain HB2. Cultures were induced at 37°C, OD 600 = 0.6-0.9, and final concentration of 0.25 mM IPTG. By comparing with the authoritative sabinene (A, B) , the capacities of sabinene biosynthesis were verified. A , C , total ion chromatogram (TIC); C , D , mass spectrum. Based on the relative retention time and total ion mass spectral comparison with an external standard, sabinene production was identified.

Figure Legend Snippet: GC-MS analysis of sabinene from the headspace of the sealed cultures of strain HB2. Cultures were induced at 37°C, OD 600 = 0.6-0.9, and final concentration of 0.25 mM IPTG. By comparing with the authoritative sabinene (A, B) , the capacities of sabinene biosynthesis were verified. A , C , total ion chromatogram (TIC); C , D , mass spectrum. Based on the relative retention time and total ion mass spectral comparison with an external standard, sabinene production was identified.

Techniques Used: Gas Chromatography-Mass Spectrometry, Concentration Assay

Effect of GPP synthase and metabolic pathway on sabinene production. A : Effect of GPP synthase. GPPS1, GPP synthase form A. grandis ; IspA, GPP synthase from E. coli . B : Effect of metabolic pathway on sabinene production, the pathway details were described in section Figure 1 . The strain harboring GPPS2 can produce 2.2-fold higher concentration of sabinene than IspA, while the strain harboring MVA pathway can produce 20-fold higher concentration of sabinene than MEP pathway.

Figure Legend Snippet: Effect of GPP synthase and metabolic pathway on sabinene production. A : Effect of GPP synthase. GPPS1, GPP synthase form A. grandis ; IspA, GPP synthase from E. coli . B : Effect of metabolic pathway on sabinene production, the pathway details were described in section Figure 1 . The strain harboring GPPS2 can produce 2.2-fold higher concentration of sabinene than IspA, while the strain harboring MVA pathway can produce 20-fold higher concentration of sabinene than MEP pathway.

Techniques Used: Concentration Assay

Effects of fermentation source and culture conditions on sabinene production by HB4. A : Effect of nitrogen sources on sabinene production; B : Effect of carbon sources on sabinene production; C : Effect of temperatures on sabinene production; D : Effect of the concentration of inducer on sabinene production. When OD 600 reached 0.6-0.9, cultures were induced for 24 h using IPTG in shake-flasks. All the experiments were performed in triplicates. Optimized conditions: Nitrogen sources, beef power; Carbon source, glycerol; Temperature, 31°C; IPTC concentration, 0.1 mM.

Figure Legend Snippet: Effects of fermentation source and culture conditions on sabinene production by HB4. A : Effect of nitrogen sources on sabinene production; B : Effect of carbon sources on sabinene production; C : Effect of temperatures on sabinene production; D : Effect of the concentration of inducer on sabinene production. When OD 600 reached 0.6-0.9, cultures were induced for 24 h using IPTG in shake-flasks. All the experiments were performed in triplicates. Optimized conditions: Nitrogen sources, beef power; Carbon source, glycerol; Temperature, 31°C; IPTC concentration, 0.1 mM.

Techniques Used: Concentration Assay

The time course of sabinene production by HB4 harboring pHB7 and pTrcLower in fed-batch fermentation. sabinene accumulation (■) and cell growth (▲). Induction was carried out at 12 h at the OD 600 of 11. The maximum concentration of sabinene was 2.65 g/L with an average productivity of 0.018 g h -1 g -1 dry cells, and the conversion efficiency of glycerol to sabinene (gram to gram) was 3.49%.

Figure Legend Snippet: The time course of sabinene production by HB4 harboring pHB7 and pTrcLower in fed-batch fermentation. sabinene accumulation (■) and cell growth (▲). Induction was carried out at 12 h at the OD 600 of 11. The maximum concentration of sabinene was 2.65 g/L with an average productivity of 0.018 g h -1 g -1 dry cells, and the conversion efficiency of glycerol to sabinene (gram to gram) was 3.49%.

Techniques Used: Concentration Assay

Sabinene biosynthesis pathway. Gene symbols and the enzymes they encode (all genes marked with black arrows were from E. faecalis , all genes marked with white arrows were isolated from S. cerevisiae , the gene marked with gray arrows and black characters were derived from A. grandis or S. pomifera , and the gene marked with gray arrows and white characters were native genes in E. coli ). Enzymes in MVA pathway: MvaE, acetyl-CoA acetyltransferase/HMG-CoA reductase; MvaS, HMG-CoA synthase; ERG12, mevalonate kinase; ERG8, phosphomevalonate kinase; ERG19, mevalonate pyrophosphate decarboxylase; IDI, IPP isomerase; A. grandis geranyl diphosphate synthase (GPPS2) and S. pomifera sabinene synthase (SabS1) were optimized to the preferred codon usage of E. coli . Enzymes in MEP pathway: DXS, DXP synthase; DXR, DXP reductoisomerase; MCT, CDP-ME synthase; CMK, CDP-ME kinase; MDS, ME-cPP synthase; HDS, HMBPP synthase: HDR, HMBPP reductase; IDI, IPP isomerase. Intermediates in MVA pathway: A-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; Mev-P, mevalonate 5-phosphate; Mev-PP, mevalonate pyrophosphate. IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate; GPP, geranyl diphosphate. Intermediates in MEP pathway: G3P, glyceraldehyde 3-phosphate; DXP, 1-deoxy-D-xylulose 5-phosphate; MEP, 2-C-methyl-D-erythritol 4-phosphate; CDP-ME, 4-(cytidine-5′-diphospho)-2-C-methyl-D-erythritol; CDP-MEP, 2-phospho-4-(cytidine-5′-di-phospho)-2-C-methyl-D-erythritol; ME-cPP, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; HMBPP, 4-hydroxy-3-methylbut-2-enyl diphosphate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate.

Figure Legend Snippet: Sabinene biosynthesis pathway. Gene symbols and the enzymes they encode (all genes marked with black arrows were from E. faecalis , all genes marked with white arrows were isolated from S. cerevisiae , the gene marked with gray arrows and black characters were derived from A. grandis or S. pomifera , and the gene marked with gray arrows and white characters were native genes in E. coli ). Enzymes in MVA pathway: MvaE, acetyl-CoA acetyltransferase/HMG-CoA reductase; MvaS, HMG-CoA synthase; ERG12, mevalonate kinase; ERG8, phosphomevalonate kinase; ERG19, mevalonate pyrophosphate decarboxylase; IDI, IPP isomerase; A. grandis geranyl diphosphate synthase (GPPS2) and S. pomifera sabinene synthase (SabS1) were optimized to the preferred codon usage of E. coli . Enzymes in MEP pathway: DXS, DXP synthase; DXR, DXP reductoisomerase; MCT, CDP-ME synthase; CMK, CDP-ME kinase; MDS, ME-cPP synthase; HDS, HMBPP synthase: HDR, HMBPP reductase; IDI, IPP isomerase. Intermediates in MVA pathway: A-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; Mev-P, mevalonate 5-phosphate; Mev-PP, mevalonate pyrophosphate. IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate; GPP, geranyl diphosphate. Intermediates in MEP pathway: G3P, glyceraldehyde 3-phosphate; DXP, 1-deoxy-D-xylulose 5-phosphate; MEP, 2-C-methyl-D-erythritol 4-phosphate; CDP-ME, 4-(cytidine-5′-diphospho)-2-C-methyl-D-erythritol; CDP-MEP, 2-phospho-4-(cytidine-5′-di-phospho)-2-C-methyl-D-erythritol; ME-cPP, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; HMBPP, 4-hydroxy-3-methylbut-2-enyl diphosphate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate.

Techniques Used: Isolation, Derivative Assay, Conditioned Place Preference

3) Product Images from ""

Article Title:

Journal: Molecular Pharmacology

doi: 10.1124/mol.114.097014

Superimposed structures of CYP2B6-sabinene and CYP2B6-(+)-3-carene and representation of active-site cavity volume. (A) Overlay of CYP2B6-sabinene (green) and CYP2B6-(+)-3-carene (red) structures in a cartoon representation showing active-site residues

Figure Legend Snippet: Superimposed structures of CYP2B6-sabinene and CYP2B6-(+)-3-carene and representation of active-site cavity volume. (A) Overlay of CYP2B6-sabinene (green) and CYP2B6-(+)-3-carene (red) structures in a cartoon representation showing active-site residues

Techniques Used:

Figure Legend Snippet: Binding Interactions of Sabinene with CYP2B6 and CYP2A6.

Techniques Used: Binding Assay

Overlay of CYP2B6-sabinene (red) and CYP2A6-sabinene (cyan) structures. Sabinene in CYP2B6 is shown in red stick representation; sabinene from CYP2A6 is shown in cyan sticks. Sabinene in CYP2B6 is shown in purple sticks and that in CYP2A6 is shown in

Figure Legend Snippet: Overlay of CYP2B6-sabinene (red) and CYP2A6-sabinene (cyan) structures. Sabinene in CYP2B6 is shown in red stick representation; sabinene from CYP2A6 is shown in cyan sticks. Sabinene in CYP2B6 is shown in purple sticks and that in CYP2A6 is shown in

Techniques Used:

Calorimetric titrations (upper panels) and binding isotherms (lower panels) of sabinene binding to (A) CYP2B6 or to (B) CYP2A6 at 25°C.

Figure Legend Snippet: Calorimetric titrations (upper panels) and binding isotherms (lower panels) of sabinene binding to (A) CYP2B6 or to (B) CYP2A6 at 25°C.

Techniques Used: Binding Assay

4) Product Images from "Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum integrifolium"

Article Title: Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum integrifolium

Journal: Toxins

doi: 10.3390/toxins11100598

Figure Legend Snippet: Chemical structures of sabinene and eucalyptol.

Techniques Used:

Phytotoxic effects of the essential oils of D. integrifolium and their major constituents, sabinene, eucalyptol, and their mixture on root growth of A. retroflexus examined by ANOVA ( p

Figure Legend Snippet: Phytotoxic effects of the essential oils of D. integrifolium and their major constituents, sabinene, eucalyptol, and their mixture on root growth of A. retroflexus examined by ANOVA ( p

Techniques Used:

Phytotoxic effects of the essential oils of D. integrifolium and their major constituents, sabinene, eucalyptol, and their mixture on shoot growth of A. retroflexus examined by ANOVA ( p

Figure Legend Snippet: Phytotoxic effects of the essential oils of D. integrifolium and their major constituents, sabinene, eucalyptol, and their mixture on shoot growth of A. retroflexus examined by ANOVA ( p

Techniques Used:

Percent mortality of A. pomi adults after treatment with of eucalyptol and sabinene mixture (65.1: 10.5) according to doses exposure and treatment times.

Figure Legend Snippet: Percent mortality of A. pomi adults after treatment with of eucalyptol and sabinene mixture (65.1: 10.5) according to doses exposure and treatment times.

Techniques Used:

Figure Legend Snippet: Percent mortality of A. pomi adults after treatment with sabinene according to doses exposure and treatment times.

Techniques Used:

IC 50 values of D. integrifolium essential oils and their major constituents, sabinene, eucalyptol, and their mixture on root and shoot length of A. retroflexus and P. annua.

Figure Legend Snippet: IC 50 values of D. integrifolium essential oils and their major constituents, sabinene, eucalyptol, and their mixture on root and shoot length of A. retroflexus and P. annua.

Techniques Used:

5) Product Images from ""

Article Title:

Journal: Molecular Pharmacology

doi: 10.1124/mol.114.097014

Techniques Used:

Figure Legend Snippet: Binding Interactions of Sabinene with CYP2B6 and CYP2A6.

Techniques Used: Binding Assay

Techniques Used:

Figure Legend Snippet: Calorimetric titrations (upper panels) and binding isotherms (lower panels) of sabinene binding to (A) CYP2B6 or to (B) CYP2A6 at 25°C.

Techniques Used: Binding Assay

6) Product Images from "Modulation of Human Neutrophil Responses by the Essential Oils from Ferula akitschkensis and their Constituents"

Article Title: Modulation of Human Neutrophil Responses by the Essential Oils from Ferula akitschkensis and their Constituents

Journal: Journal of agricultural and food chemistry

doi: 10.1021/acs.jafc.6b03205

Chemical structures of the compounds evaluated for biological activity. For sabinene and geranylacetone, mixtures of stereoisomers were studied; general structures are shown for these compounds.

Figure Legend Snippet: Chemical structures of the compounds evaluated for biological activity. For sabinene and geranylacetone, mixtures of stereoisomers were studied; general structures are shown for these compounds.

Techniques Used: Activity Assay

Pharmacophore modeling based on active constituents of essential oils and known agonists of TRP channels. Panel A . Three-molecule template A created by alignment of (−)-β-pinene (magenta), (−)-sabinene (light-green), and γ-terpinene

Figure Legend Snippet: Pharmacophore modeling based on active constituents of essential oils and known agonists of TRP channels. Panel A . Three-molecule template A created by alignment of (−)-β-pinene (magenta), (−)-sabinene (light-green), and γ-terpinene

Techniques Used:

Effect of selected compounds on f MLF- and IL-8-induced [Ca 2+ ] i mobilization. Human neutrophils were loaded with calcium dye and pretreated with 50 µg/ml FAEO u/s ( Panel A ), 50 µM sabinene ( Panel B ), 50 µM geranylacetone ( Panel C

Figure Legend Snippet: Effect of selected compounds on f MLF- and IL-8-induced [Ca 2+ ] i mobilization. Human neutrophils were loaded with calcium dye and pretreated with 50 µg/ml FAEO u/s ( Panel A ), 50 µM sabinene ( Panel B ), 50 µM geranylacetone ( Panel C

Techniques Used:

7) Product Images from "Hydrodistillation Extraction Kinetics Regression Models for Essential Oil Yield and Composition in Juniperus virginiana, J. excelsa, and J. sabina"

Article Title: Hydrodistillation Extraction Kinetics Regression Models for Essential Oil Yield and Composition in Juniperus virginiana, J. excelsa, and J. sabina

Journal: Molecules

doi: 10.3390/molecules24050986

Plot of Distillation time vs. the essential oil (EO) content and the concentrations of 7 constituents along with the fitted Power (convex) and Asymptotic (convex) nonlinear regression models for Juniperus sabina . The fitted models are: Y ^ = 0.89 D T − 0.59 (EO content), Y ^ = 1.64 D T − 0.22 (α-thujene), Y ^ = 4.0 D T − 0.31 (α-pinene), Y ^ = 94.8 D T − 0.3 (Sabinene), Y ^ = 4.67 D T − 0.28 (β-pinene), Y ^ = 2.38 D T − 0.11 (limonene), Y ^ = 5.16 − 36.3 ( E x p ( − 0.696 D T ) ) (Terpinen-4-ol), and Y ^ = 9.94 − 10.3 ( E x p ( − 0.087 D T ) ) (Methyl Eugenol).

Figure Legend Snippet: Plot of Distillation time vs. the essential oil (EO) content and the concentrations of 7 constituents along with the fitted Power (convex) and Asymptotic (convex) nonlinear regression models for Juniperus sabina . The fitted models are: Y ^ = 0.89 D T − 0.59 (EO content), Y ^ = 1.64 D T − 0.22 (α-thujene), Y ^ = 4.0 D T − 0.31 (α-pinene), Y ^ = 94.8 D T − 0.3 (Sabinene), Y ^ = 4.67 D T − 0.28 (β-pinene), Y ^ = 2.38 D T − 0.11 (limonene), Y ^ = 5.16 − 36.3 ( E x p ( − 0.696 D T ) ) (Terpinen-4-ol), and Y ^ = 9.94 − 10.3 ( E x p ( − 0.087 D T ) ) (Methyl Eugenol).

Techniques Used: Distillation

8) Product Images from "Defense traits in the long‐lived Great Basin bristlecone pine and resistance to the native herbivore mountain pine beetle"

Article Title: Defense traits in the long‐lived Great Basin bristlecone pine and resistance to the native herbivore mountain pine beetle

Journal: The New Phytologist

doi: 10.1111/nph.14191

Non‐metric multidimensional scaling ( NMDS ) ordination of relative concentration (dimensions = 2, stress = 0.129) in Great Basin ( GB ) bristlecone, foxtail and limber pines. Squares, species means; ellipses, 95th percentile confidence interval ( CI ) of species mountain range location in ordination space, where: C, Cedar; K, Klamath; R, Ruby; S, Spring; SN , Sierra Nevada; W, White (see Fig. 1 ; Supporting Information Table S1). (−)‐α‐Pinene, NAP ; (+)‐α‐pinene, PAP ; (−)‐β‐pinene, NBP ; R‐(+)‐limonene, LIM ; β‐phellandrene, BPH ; terpinolene, TER ; (−)‐camphene, NCA ; (+)‐β‐pinene, PBP ; (+)‐camphene, PCA ; myrcene, MYR ; sabinene, SAB ; γ‐terpinene, YTE ; (+)‐Δ‐3‐carene, CAR ; tricyclene, TRI ; monoterpene 1, MT 1; monoterpene 2, MT 2; monoterpene 3, MT 3; monoterpene 4, MT 4; monoterpene 5, MT 5; monoterpene 6, MT 6; monoterpene 7, MT 7; monoterpene 8, MT 8; 4‐allylanisole, ALY ; unknown 1, UK 1; hydrocarbon 1, HC 1; hydrocarbon 2, HC 2.

Figure Legend Snippet: Non‐metric multidimensional scaling ( NMDS ) ordination of relative concentration (dimensions = 2, stress = 0.129) in Great Basin ( GB ) bristlecone, foxtail and limber pines. Squares, species means; ellipses, 95th percentile confidence interval ( CI ) of species mountain range location in ordination space, where: C, Cedar; K, Klamath; R, Ruby; S, Spring; SN , Sierra Nevada; W, White (see Fig. 1 ; Supporting Information Table S1). (−)‐α‐Pinene, NAP ; (+)‐α‐pinene, PAP ; (−)‐β‐pinene, NBP ; R‐(+)‐limonene, LIM ; β‐phellandrene, BPH ; terpinolene, TER ; (−)‐camphene, NCA ; (+)‐β‐pinene, PBP ; (+)‐camphene, PCA ; myrcene, MYR ; sabinene, SAB ; γ‐terpinene, YTE ; (+)‐Δ‐3‐carene, CAR ; tricyclene, TRI ; monoterpene 1, MT 1; monoterpene 2, MT 2; monoterpene 3, MT 3; monoterpene 4, MT 4; monoterpene 5, MT 5; monoterpene 6, MT 6; monoterpene 7, MT 7; monoterpene 8, MT 8; 4‐allylanisole, ALY ; unknown 1, UK 1; hydrocarbon 1, HC 1; hydrocarbon 2, HC 2.

Techniques Used: Concentration Assay

Activity Assay:

Article Title: Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum integrifolium
Article Snippet: .. Phytotoxic Activity of the Essential Oils and Their Major Constituents The major constituents of the essential oil, i.e., sabinene and eucalyptol, were purchased from Sigma-Aldrich Co. (St. Louis, USA). ..

Concentration Assay:

Article Title: Microbial production of sabinene--a new terpene-based precursor of advanced biofuel
Article Snippet: .. The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of sabinene which was bought from Sigma-Aldrich. .. Optimization of fermentation medium and process Optimization of fermentation medium was performed in shake-flask experiments in triplicate series of 600 ml sealed shake flasks containing 50 ml of fermentation medium incubated with the strain HB4.

Article Title:
Article Snippet: .. Sabinene or (+)-3-carene for CYP2B6 and sabinene for CYP2A6 was added to a final concentration of 180 μ M, and the samples were concentrated to 280 and 150 μ M, respectively, by centrifugation using 50-kDa cutoff Amicon ultrafiltration devices (Millipore). .. The protein aliquots were again diluted to 18 μ M using the above buffer containing ligand at a final concentration of 180 μ M. The procedure was repeated twice before concentrating the protein to the final concentration of 280 μ M for CYP2B6 and 150 μ M for CYP2A6.

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies
Article Snippet: After 24 h of fermentation, 5 mL cyclohexane was injected into each bottle and the culture was continued incubated in the shaker at 30 °C 200 rpm for additional 16 h. Culture was centrifuged and 1 μL upper organic phase was analyzed by gas chromatography (GC) using an Agilent 7890B GC system equipped with a DB-5MS column. .. The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich. .. Genome resequencing analysis Two clones from each of the evolved strain XYF(DE3) and the parental strain BL21(DE3) were genome sequenced through Illumina HiSeq.

Centrifugation:

other:

Article Title: Modulation of Human Neutrophil Responses by the Essential Oils from Ferula akitschkensis and their Constituents
Article Snippet: Sabinene, p -cymen-8-ol, camphene, α-pinene oxide, α-terpineol, γ-terpinene, 4-carvomenthenol (terpinen-4-ol), myrtenol, and 3,7-dimethyl-1,3,6-octatriene (β-ocimene) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).

sabinene (Millipore)

Structured Review

Images

1) Product Images from "The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases"

2) Product Images from "Microbial production of sabinene--a new terpene-based precursor of advanced biofuel"

3) Product Images from ""

4) Product Images from "Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum integrifolium"

5) Product Images from ""

6) Product Images from "Modulation of Human Neutrophil Responses by the Essential Oils from Ferula akitschkensis and their Constituents"

7) Product Images from "Hydrodistillation Extraction Kinetics Regression Models for Essential Oil Yield and Composition in Juniperus virginiana, J. excelsa, and J. sabina"

8) Product Images from "Defense traits in the long‐lived Great Basin bristlecone pine and resistance to the native herbivore mountain pine beetle"

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