rabbit anti rpa2 phospho s4 s8 (Bethyl)
Structured Review

Rabbit Anti Rpa2 Phospho S4 S8, supplied by Bethyl, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti rpa2 phospho s4 s8/product/Bethyl
Average 86 stars, based on 1 article reviews
Images
1) Product Images from "The interplay of the translocase activity and protein recruitment function of PICH in ultrafine anaphase bridge resolution and genomic stability"
Article Title: The interplay of the translocase activity and protein recruitment function of PICH in ultrafine anaphase bridge resolution and genomic stability
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkae1249

Figure Legend Snippet: PICH plays both catalytic and protein recruitment function in UFB resolution. ( A ) RPE1 PICH-AG cells expressing GFP-PICH constructs were treated with Dox and IAA for 72 h. Cell extracts were analyzed by western blotting with the indicated antibodies. ( B ) Clonogenic cell survival assays were carried out on RPE1 PICH-AG cells expressing different GFP-PICH constructs treated with Dox and IAA. ( C ) RPE1 PICH-AG expressing GFP-PICH WT or GFP-PICH K128A were treated with Dox and IAA for 24 h. GFP, histone H3 pS10 and DNA were visualized using anti-GFP (green), anti-histone H3 pS10 (red) antibodies and DAPI (blue), respectively. ( D ) Quantification of the number of PICH-coated UFBs and chromatin bridges per cells (90 cells per condition) as visualized in panel (C). ( E ) RPE1 PICH-AG expressing GFP-PICH WT , GFP-PICH K128A or GFP- PICH Δ791–1000 were treated with Dox and IAA for 24 h. Cells were then treated with ICRF-193 (25 nM, 2 h) before fixed. GFP, RPA2 and DNA were visualized using anti-GFP (green), anti-RPA2 (red) antibodies and DAPI (blue), respectively. Scale bars, 10 μm. ( F ) Quantification of the number of PICH-coated and RPA2-coated UFBs per cells (90 cells per condition) as visualized in panel (E). ( G ) HeLa TOP3A-GFP cells were transfected with the indicated siRNAs for 48 h. Cell extracts were analyzed by western blotting with the indicated antibodies. ( H ) Cells in panel (G) were treated with ICRF-193 (50 nM) for 2 h. GFP, PICH and DNA were visualized using anti-GFP (green), anti-PICH (red) antibodies and DAPI (blue), respectively. ( I ) Quantification of the number of PICH-coated and TOP3A-coated UFBs per cells (>50 cells per condition) as visualized in panel (H). In all quantified data, bars represent the mean ± SD of n = 3 independent experiments. ** P < 0.01; **** P < 0.0001; ns (not significant) P > 0.05; statistical significance values were determined with unpaired two-tailed t -tests.
Techniques Used: Expressing, Construct, Western Blot, Transfection, Two Tailed Test