s220  (Covaris)


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    Structured Review

    Covaris s220
    S220, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s220/product/Covaris
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    s220 - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Sonication:

    Article Title: Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages
    Article Snippet: .. For CDK9, nuclei were sonicated with Covaris S220 Ultrasonicator according to manufacturer’s instructions (130 μl shearing buffer, 200 cycles/burst, 120 s, DF 10). .. Lysates were cleared by centrifugation at 14,000*g, 20 min, 4°C, and then incubated with normal rabbit IgG (Santa Cruz Biotech, sc-2027x), BRD4 (Abcam, ab84776 and Bethyl Laboratories, A3001-985A100), MED12 (Bethyl Laboratories, A300-774A), MED1 (Bethyl Laboratories, A300-793), p300 (Santa Cruz Biotech, sc-585X), Anti-AcH4 (Millipore, 06–866), Anti-AcH4K12 (Millipore, 07–595), Anti-AcH4K5 (Millipore, 07–327) and 40 μl of 50% protein A/G plus agarose (Santa Cruz Biotech, sc-2003) per reaction at 4°C ON.

    Article Title: Loss of Tet enzymes compromises proper differentiation of embryonic stem cells
    Article Snippet: .. 2 μg of genomic DNA isolated from day 10 EBs was sonicated to 300bp fragments using a Covaris S220 ultrasonicator (Covaris). .. One μg of purified adapter ligated DNA was used for pooled immunoprecipitations using a polyclonal 5mC-specific antibody (Active Motif, 39791).

    Purification:

    Article Title: Functional and evolutionary insights from the Ciona notochord transcriptome
    Article Snippet: .. Purified RNA (2.5 μl) was used in half-reactions following their standard protocol. cDNA samples were fragmented with a Covaris S220 Ultrasonicator using the recommended settings to obtain an average fragment length of 300 bp and the NEBNext DNA Library Prep Master Mix set for Illumina from New England BioLabs (NEB) was used for library construction. .. The resulting libraries were quality checked with an Agilent 2100 Bioanalyzer and quantified by RT-PCR.

    Article Title: Pancreatic islet chromatin accessibility and conformation reveals distal enhancer networks of type 2 diabetes risk
    Article Snippet: .. DNA was purified with phenol-chloroform extraction and ethanol precipitation, followed by fragmentation to 300–500 bp with the Covaris S220 ultrasonicator. .. Ligation products were enriched with Dynabeads My One T1 Streptavidin beads (Life Technologies).

    Isolation:

    Article Title: Loss of Tet enzymes compromises proper differentiation of embryonic stem cells
    Article Snippet: .. 2 μg of genomic DNA isolated from day 10 EBs was sonicated to 300bp fragments using a Covaris S220 ultrasonicator (Covaris). .. One μg of purified adapter ligated DNA was used for pooled immunoprecipitations using a polyclonal 5mC-specific antibody (Active Motif, 39791).

    Ethanol Precipitation:

    Article Title: Pancreatic islet chromatin accessibility and conformation reveals distal enhancer networks of type 2 diabetes risk
    Article Snippet: .. DNA was purified with phenol-chloroform extraction and ethanol precipitation, followed by fragmentation to 300–500 bp with the Covaris S220 ultrasonicator. .. Ligation products were enriched with Dynabeads My One T1 Streptavidin beads (Life Technologies).

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    Covaris covaris s220 sonicator
    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a <t>Covaris</t> <t>S220</t> sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.
    Covaris S220 Sonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris s220 sonicator/product/Covaris
    Average 99 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    covaris s220 sonicator - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    85
    Covaris acoustic solubilizer s220
    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a <t>Covaris</t> <t>S220</t> sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.
    Acoustic Solubilizer S220, supplied by Covaris, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acoustic solubilizer s220/product/Covaris
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acoustic solubilizer s220 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    86
    Covaris s220 adaptive focused acoustics ultra sonicator
    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a <t>Covaris</t> <t>S220</t> sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.
    S220 Adaptive Focused Acoustics Ultra Sonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s220 adaptive focused acoustics ultra sonicator/product/Covaris
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s220 adaptive focused acoustics ultra sonicator - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)

    doi: 10.1007/978-1-4939-7768-0_12

    Figure Lengend Snippet: Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Article Snippet: Note 5 If using the Covaris S220 sonicator, no more than 10 μg of DNA should be fragmented at a time; if you obtained more than 10 μg of DNA from the treated cells, you should dilute to 100 ng/ul and only use 8–10 μg per sonication tube.

    Techniques: Chromatin Immunoprecipitation, Labeling