s2 sonicator  (Covaris)


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  • 97

    Structured Review

    Covaris s2 sonicator
    S2 Sonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 97/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s2 sonicator/product/Covaris
    Average 97 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    s2 sonicator - by Bioz Stars, 2020-01
    97/100 stars

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    Related Articles

    Sequencing:

    Article Title:
    Article Snippet: .. Library preparation and exome sequencing Genomic DNA was fragmented using an S2 sonicator (Covaris, USA). .. Whole genome libraries were constructed using a NEBNext Ultra DNA Library kit (New England Biosciences, Ipswich, MA) with local modifications (specifically Ion Torrent sequencing adaptors were added) and the exome was enriched using a SureSelectXT Mouse All Exon kit (Agilent, Mulgrave, Vic, Australia) that targets 49.6 Mb of the murine genome (221,784 exons across 24,306 coding genes).

    Next-Generation Sequencing:

    Article Title:
    Article Snippet: In brief, 5 μg DNA in 120 μl EB buffer was sheared to≈150 bp using a S2 sonicator (Covaris, Woburn, MA, USA) for 6 cycles (duty cycle 20%, intensity 5, burst per cycle 200, time: 60 s). .. DNA eluted with the highest salt concentration was submitted to the DKFZ Genomics and Proteomics Core Facility for library preparation and next generation sequencing on an Ilumina HiSeq 2000 sequencer (single read 50 bp).

    Construct:

    Article Title:
    Article Snippet: Library preparation and exome sequencing Genomic DNA was fragmented using an S2 sonicator (Covaris, USA). .. Whole genome libraries were constructed using a NEBNext Ultra DNA Library kit (New England Biosciences, Ipswich, MA) with local modifications (specifically Ion Torrent sequencing adaptors were added) and the exome was enriched using a SureSelectXT Mouse All Exon kit (Agilent, Mulgrave, Vic, Australia) that targets 49.6 Mb of the murine genome (221,784 exons across 24,306 coding genes).

    DNA Methylation Assay:

    Article Title:
    Article Snippet: MCIp-seq MCIp-seq , was used for genome-wide DNA methylation analysis as in Sonnet et al. with minor modifications. .. In brief, 5 μg DNA in 120 μl EB buffer was sheared to≈150 bp using a S2 sonicator (Covaris, Woburn, MA, USA) for 6 cycles (duty cycle 20%, intensity 5, burst per cycle 200, time: 60 s).

    Concentration Assay:

    Article Title:
    Article Snippet: In brief, 5 μg DNA in 120 μl EB buffer was sheared to≈150 bp using a S2 sonicator (Covaris, Woburn, MA, USA) for 6 cycles (duty cycle 20%, intensity 5, burst per cycle 200, time: 60 s). .. DNA eluted with the highest salt concentration was submitted to the DKFZ Genomics and Proteomics Core Facility for library preparation and next generation sequencing on an Ilumina HiSeq 2000 sequencer (single read 50 bp).

    Article Title:
    Article Snippet: The fixation was qu enched by addition of glycine (Sigma) at a final concentration of 125mM. .. The sonication was performed on S2 sonicator (Covaris) at duty cycle 20%, intensity 5, cycle/burst 200 for 30 minutes.

    Incubation:

    Article Title:
    Article Snippet: The sonication was performed on S2 sonicator (Covaris) at duty cycle 20%, intensity 5, cycle/burst 200 for 30 minutes. .. Ten μg of anti-RPA32 (EMD, NA19L), anti-RAD51 (Santa Cruz, H-92) or anti-γH2AX (Epitomics, 2212-1) was incubated with 40 μl of Dynabeads Protein A for 40 minutes at room temperature.

    Cell Culture:

    Article Title:
    Article Snippet: Ex-vivo cultured B-cells or thymocytes were fixed with 1% formaldehyde (Sigma) for 10 minutes at 37°C. .. The sonication was performed on S2 sonicator (Covaris) at duty cycle 20%, intensity 5, cycle/burst 200 for 30 minutes.

    Genome Wide:

    Article Title:
    Article Snippet: MCIp-seq MCIp-seq , was used for genome-wide DNA methylation analysis as in Sonnet et al. with minor modifications. .. In brief, 5 μg DNA in 120 μl EB buffer was sheared to≈150 bp using a S2 sonicator (Covaris, Woburn, MA, USA) for 6 cycles (duty cycle 20%, intensity 5, burst per cycle 200, time: 60 s).

    Ex Vivo:

    Article Title:
    Article Snippet: Ex-vivo cultured B-cells or thymocytes were fixed with 1% formaldehyde (Sigma) for 10 minutes at 37°C. .. The sonication was performed on S2 sonicator (Covaris) at duty cycle 20%, intensity 5, cycle/burst 200 for 30 minutes.

    Sonication:

    Article Title:
    Article Snippet: .. The sonication was performed on S2 sonicator (Covaris) at duty cycle 20%, intensity 5, cycle/burst 200 for 30 minutes. .. Ten μg of anti-RPA32 (EMD, NA19L), anti-RAD51 (Santa Cruz, H-92) or anti-γH2AX (Epitomics, 2212-1) was incubated with 40 μl of Dynabeads Protein A for 40 minutes at room temperature.

    Chromatin Immunoprecipitation:

    Article Title:
    Article Snippet: Library preparation and exome sequencing Genomic DNA was fragmented using an S2 sonicator (Covaris, USA). .. Two samples were pooled and sequenced on a P1 chip for 520 flows to an average depth of 42×, which resulted in 80% of the targeted regions being covered with at least an average depth of 13.2× (See Additional file ).

    Article Title:
    Article Snippet: In brief, 5 μg DNA in 120 μl EB buffer was sheared to≈150 bp using a S2 sonicator (Covaris, Woburn, MA, USA) for 6 cycles (duty cycle 20%, intensity 5, burst per cycle 200, time: 60 s). .. Size distribution was confirmed on a DNA High sensitivity Chip (Agilent Bioanalyzer), before proceeding with MCIp reaction using a SX8G-V52 robot (Diagenode, Liège, Belgium) for automated processing.

    ChIP-sequencing:

    Article Title:
    Article Snippet: Paragraph title: ChIP-seq ... The sonication was performed on S2 sonicator (Covaris) at duty cycle 20%, intensity 5, cycle/burst 200 for 30 minutes.

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  • 99
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    Covaris S2 Sonication, supplied by Covaris, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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