s2 adaptive  (Covaris)


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    Structured Review

    Covaris s2 adaptive
    S2 Adaptive, supplied by Covaris, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s2 adaptive/product/Covaris
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    s2 adaptive - by Bioz Stars, 2019-10
    82/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes
    Article Snippet: Paragraph title: Selective cDNA synthesis: the Ovation Prokaryotic RNA-Seq System ... The cDNA was sheared using the Covaris S2 adaptive focused acoustics instrument (Covaris, Woburn, MA, USA) with the following conditions: duty cycle 5%, intensity 10, cycles/burst 200, time 6 minutes.

    Article Title: Differential RNA Sequencing Implicates Sulfide as the Master Regulator of S0 Metabolism in Chlorobaculum tepidum and Other Green Sulfur Bacteria
    Article Snippet: RNA was extracted using a NucleoSpin RNA kit (Macherey-Nagel), and rRNA was depleted using a MicrobExpress kit (Ambion) and treated with a TURBO DNA-free kit (Ambion) to remove residual genomic DNA (gDNA). .. RNA-seq libraries were constructed using a NuGEN Ovation kit (NuGEN) to convert 25 ng RNA to double-stranded cDNA, with subsequent fragmentation to approximately 100-bp fragments performed using an S2 Adaptive Focused Acoustic Disruptor (Covaris). .. Fragment size was confirmed by agarose gel electrophoresis on a 2% gel.

    Construct:

    Article Title: Differential RNA Sequencing Implicates Sulfide as the Master Regulator of S0 Metabolism in Chlorobaculum tepidum and Other Green Sulfur Bacteria
    Article Snippet: RNA was extracted using a NucleoSpin RNA kit (Macherey-Nagel), and rRNA was depleted using a MicrobExpress kit (Ambion) and treated with a TURBO DNA-free kit (Ambion) to remove residual genomic DNA (gDNA). .. RNA-seq libraries were constructed using a NuGEN Ovation kit (NuGEN) to convert 25 ng RNA to double-stranded cDNA, with subsequent fragmentation to approximately 100-bp fragments performed using an S2 Adaptive Focused Acoustic Disruptor (Covaris). .. Fragment size was confirmed by agarose gel electrophoresis on a 2% gel.

    Purification:

    Article Title: Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes
    Article Snippet: The cDNA was purified using a MinElute column (QIAGEN) and eluted in 1× low TE (10 mM Tris, 0.1 mM EDTA, pH 8.0). .. The cDNA was sheared using the Covaris S2 adaptive focused acoustics instrument (Covaris, Woburn, MA, USA) with the following conditions: duty cycle 5%, intensity 10, cycles/burst 200, time 6 minutes.

    Article Title:
    Article Snippet: After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter. .. After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter.

    Real-time Polymerase Chain Reaction:

    Article Title: Prox1 and fibroblast growth factor receptors form a novel regulatory loop controlling lens fiber differentiation and gene expression
    Article Snippet: Nuclei were extracted and processed using the truChIP Chromatin Shearing Kit (Covaris) and chromatin sheared using a Covaris S2 adaptive focused acoustic disrupter. .. ChIP was carried out using 100 µg chromatin per sample, pulling down with 1 µl anti-Prox1 ( ).

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter. .. After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter.

    Incubation:

    Article Title: Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes
    Article Snippet: The cDNA was sheared using the Covaris S2 adaptive focused acoustics instrument (Covaris, Woburn, MA, USA) with the following conditions: duty cycle 5%, intensity 10, cycles/burst 200, time 6 minutes. .. The cDNA was sheared using the Covaris S2 adaptive focused acoustics instrument (Covaris, Woburn, MA, USA) with the following conditions: duty cycle 5%, intensity 10, cycles/burst 200, time 6 minutes.

    Article Title:
    Article Snippet: ChIP assays were performed as previously described with minor variations (see ) ( ). .. After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter. .. Samples were immunoprecipitated with antibodies against GATA1 (sc-265, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), NF-E2 (sc-22827, Santa Cruz Biotechnology, Inc.), KLF1 (ab2483, Abcam), SCL/Tal1 (sc-12984, Santa Cruz Biotechnology, Inc.), p300 (sc-585, Santa Cruz Biotechnology, Inc.), H3K4me2 (32356, Abcam), H3K4me3 (1012, Abcam), and nonspecific rabbit IgG (sc-2091, Santa Cruz Biotechnology, Inc.).

    Sequencing:

    Article Title: Differential RNA Sequencing Implicates Sulfide as the Master Regulator of S0 Metabolism in Chlorobaculum tepidum and Other Green Sulfur Bacteria
    Article Snippet: RNA-seq libraries were constructed using a NuGEN Ovation kit (NuGEN) to convert 25 ng RNA to double-stranded cDNA, with subsequent fragmentation to approximately 100-bp fragments performed using an S2 Adaptive Focused Acoustic Disruptor (Covaris). .. RNA-seq libraries were constructed using a NuGEN Ovation kit (NuGEN) to convert 25 ng RNA to double-stranded cDNA, with subsequent fragmentation to approximately 100-bp fragments performed using an S2 Adaptive Focused Acoustic Disruptor (Covaris).

    Sonication:

    Article Title:
    Article Snippet: ChIP assays were performed as previously described with minor variations (see ) ( ). .. After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter. .. Samples were immunoprecipitated with antibodies against GATA1 (sc-265, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), NF-E2 (sc-22827, Santa Cruz Biotechnology, Inc.), KLF1 (ab2483, Abcam), SCL/Tal1 (sc-12984, Santa Cruz Biotechnology, Inc.), p300 (sc-585, Santa Cruz Biotechnology, Inc.), H3K4me2 (32356, Abcam), H3K4me3 (1012, Abcam), and nonspecific rabbit IgG (sc-2091, Santa Cruz Biotechnology, Inc.).

    Chromatin Immunoprecipitation:

    Article Title:
    Article Snippet: Paragraph title: Chromatin Immunoprecipitation ... After incubation, nuclei were sonicated with the Covaris S2 adaptive focused acoustics disrupter.

    Article Title: Prox1 and fibroblast growth factor receptors form a novel regulatory loop controlling lens fiber differentiation and gene expression
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Nuclei were extracted and processed using the truChIP Chromatin Shearing Kit (Covaris) and chromatin sheared using a Covaris S2 adaptive focused acoustic disrupter.

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