Journal: Microbiology Spectrum
Article Title: Milteforan, a promising veterinary commercial product against feline sporotrichosis
doi: 10.1128/spectrum.00474-24
Figure Lengend Snippet: In vitro fungicidal activity of miltefosine and milteforan against the yeast morphology of S. schenckii and S. brasiliensis . (a) S. schenckii (strains 4820, 4821, and 4822) and (b) S. brasiliensis (strains 4823, 4824, and 4858) yeast were grown in liquid YDP pH 7.8 at 37°C in the presence of several concentrations of MFS or ML (16, 8, 4, 2, 1, 0.5, and 0.25 µg/mL). After 4 days of incubation, the cells were plated in solid YPD pH 7.8 and incubated for 4 days at 37°C. As a control, yeast cells of each strain were grown without the drugs. The results represent the average of three independent experiments performed by duplicate.
Article Snippet: In this study, three Sporothrix schenckii (ATCC-MYA 4820, ATCC-MYA 4821, and ATCC-MYA 4822) and three S. brasiliensis strains (ATCC-MYA 4823, ATCC-MYA 4824, and ATCC-MYA 4858) were used for the in vitro antifungal susceptibility assays; S. schenckii ATCC-MYA 4821 and S. brasiliensis ATCC- MYA 4823 were used for the checkerboard assays; and S. brasiliensis ATCC-MYA 4823, a highly virulent clinical isolate obtained from feline sporotrichosis , was used for the infection assays.
Techniques: In Vitro, Activity Assay, Incubation, Control