Journal: Redox Biology

Article Title: Synthetic anticoagulant octaparin targets mitochondrial cardiolipin-GSDMD axis to rescue redox homeostasis in sepsis

doi: 10.1016/j.redox.2025.103877

Figure Lengend Snippet: Octaparin attenuates systemic inflammation and organ failure to improve survival in sepsis. (A) ELISA analysis of TNF-α and IL-6 in culture supernatants from BMDMs stimulated with LPS (1 μg/mL) in the presence or absence of octaparin (5 μg/mL), heparin (5 μg/mL), enoxaparin (5 μg/mL) and fondaparinux (5 μg/mL) for 24 h. (B) ELISA analysis of TNF-α and IL-6 in culture supernatants from BMDMs stimulated with S. typhimurium (MOI = 10) in the presence or absence of octaparin (5 μg/mL), heparin (5 μg/mL), enoxaparin (5 μg/mL) and fondaparinux (5 μg/mL) for 24 h. (C–E) Kaplan-Meier survival curves (C), histopathological images of lung tissues with quantitative assessment of lung injury via scoring of histopathological features (D), and serum concentrations of TNF-α and IL-6 (E) from mice at 24 h after intraperitoneal injection of LPS (25 mg/kg) or saline. Octaparin (10 or 20 mg/kg) was administered intraperitoneally 30 min after LPS injection. Scale bar represents 50 μm. (F–H) Kaplan-Meier survival curves (F), histopathological images of lung tissues with quantitative assessment of lung injury via scoring of histopathological features (G), and serum concentrations of TNF-α and IL-6 (H) from mice at 24 h after intraperitoneal injection of S. typhimurium (2 × 10 6 CFU/20 g) or saline. Octaparin (10 or 20 mg/kg) was administered intraperitoneally 2 h after S. typhimurium injection. Scale bar represents 50 μm. (I–K) Mice received intraperitoneal injections of S. typhimurium (2x106 CFU/20 g) or sterile saline. Octaparin (20 mg/kg) was administered intraperitoneally 2 h following S. typhimurium challenge. Mice were sacrificed 48 h later for various analyses. Representative H&E-stained sections of liver, lung, and kidney tissues. Scale bars, 50 μm (I). Immunofluorescence staining for F4/80 and Cl-casp3 in liver, lung, and kidney tissues. Percentage of F4/80 + and Cl-casp3 + cells in liver, lung, and kidney tissues. Scale bar, 50 μm (J). Serum levels of LDH, ALT and creatinine (K). The graphs are shown as individual data points along with mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Statistical analyses by one-way ANOVA test and log-rank test for survival.

Article Snippet: S. typhimurium (ATCC, 14028) was procured from ATCC, while S. typhimurium-GFP was obtained from BeNa Culture Collection.

Techniques: Enzyme-linked Immunosorbent Assay, Injection, Saline, Sterility, Staining, Immunofluorescence

Journal: Redox Biology

Article Title: Synthetic anticoagulant octaparin targets mitochondrial cardiolipin-GSDMD axis to rescue redox homeostasis in sepsis

doi: 10.1016/j.redox.2025.103877

Figure Lengend Snippet: Octaparin potently enhances the phagocytic capacity of BMDMs. (A) GO enrichment analysis of upregulated genes in BMDMs following treatment with LPS plus octaparin versus LPS alone. (B) The relative phagocytic activity of BMDMs stimulated with indicated doses of octaparin for 24 h was assessed via neutral red uptake. (C) The relative phagocytic activity of BMDMs stimulated with indicated doses of octaparin for 24 h was assessed via S. typhimurium-GFP uptake. (D) BMDMs were stimulated with indicated doses of octaparin for 24 h, followed by incubation with S. typhimurium-GFP at a MOI of 10 for 6 h. Representative images are shown. Bacterial clearance capacity was evaluated by calculating the percentage decrease in extracellular fluorescence. Scale bars, 20 μm. (E) BMDMs were stimulated with indicated doses of octaparin for 24 h, followed by incubation with S. typhimurium-GFP at a MOI of 5 for 24 h. Representative confocal microscopy images show PI + GFP + cells. Scale bar, 20 μm. (F) Mice received intraperitoneal injections of S. typhimurium (2 × 10 6 CFU/20 g) or sterile saline. Octaparin (20 mg/kg) was given intraperitoneally 2 h post S. typhimurium challenge, and mice were sacrificed 48 h later for analyses. Bacterial loads in the liver, lung, and kidney were determined. The graphs are shown as individual data points along with mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Statistical analyses by one-way ANOVA test.

Article Snippet: S. typhimurium (ATCC, 14028) was procured from ATCC, while S. typhimurium-GFP was obtained from BeNa Culture Collection.

Techniques: Activity Assay, Incubation, Fluorescence, Confocal Microscopy, Sterility, Saline

Journal: MicrobiologyOpen

Article Title: Serovar‐Specific Antimicrobial Resistance and Virulence Profiles of Salmonella enterica From Poultry in Bangladesh

doi: 10.1002/mbo3.70091

Figure Lengend Snippet: Virulence profiles of Salmonella enterica serovars isolated from chickens in Bangladesh.

Article Snippet: S. enterica Typhimurium ATCC 14028 and S. enterica Enteritidis ATCC 13076 were used as positive controls, while phosphate‐buffered saline was used as a PCR‐negative control instead of genomic DNA.

Techniques: Isolation

Journal: MicrobiologyOpen

Article Title: Serovar‐Specific Antimicrobial Resistance and Virulence Profiles of Salmonella enterica From Poultry in Bangladesh

doi: 10.1002/mbo3.70091

Figure Lengend Snippet: Phenotypic antimicrobial resistance profiles of Salmonella enterica serovars isolated from chickens in Bangladesh. AK, amikacin; AMC, amoxicillin‐clavulanate; AMP, ampicillin; AT, aztreonam; AZM, azithromycin; CAZ, ceftazidime; CIP, ciprofloxacin; COT, trimethoprim‐sulfamethoxazole; CPM, cefepime; CTR, ceftriaxone; CTX, cefotaxime; DO, doxycycline; F, nitrofurantoin; FOS, fosfomycin; GEN, gentamicin; IMP, imipenem; LE, levofloxacin; MDR, multidrug resistant; MEM, meropenem; NA, nalidixic acid; S, streptomycin; S3, sulfonamide; SMX, sulfamethoxazole; TE, tetracycline; TM, sulfamethoxazole.

Article Snippet: S. enterica Typhimurium ATCC 14028 and S. enterica Enteritidis ATCC 13076 were used as positive controls, while phosphate‐buffered saline was used as a PCR‐negative control instead of genomic DNA.

Techniques: Isolation

Journal: MicrobiologyOpen

Article Title: Serovar‐Specific Antimicrobial Resistance and Virulence Profiles of Salmonella enterica From Poultry in Bangladesh

doi: 10.1002/mbo3.70091

Figure Lengend Snippet: Genotypic antimicrobial resistance profiles of Salmonella enterica serovars isolated from chickens in Bangladesh.

Article Snippet: S. enterica Typhimurium ATCC 14028 and S. enterica Enteritidis ATCC 13076 were used as positive controls, while phosphate‐buffered saline was used as a PCR‐negative control instead of genomic DNA.

Techniques: Isolation