Journal:
Article Title: The ars Detoxification System Is Advantageous but Not Required for As(V) Respiration by the Genetically Tractable Shewanella Species Strain ANA-3
doi: 10.1128/AEM.69.5.2800-2809.2003
Figure Lengend Snippet: Strains and plasmids used in this study
Article Snippet: ANA-3 Isolated from an As-treated wooden pier piling in a brackish estuary (Eel Pond, Woods Hole, Mass.), contains arsDABC , respires on As(V) resistant to >5 mM arsenite This study ARSB1 arsB mutant derived from ANA-3; arsB ::Kan(EZ::TN) This study S. oneidensis MR-1 Manganese-reducing strain from Oneida Lake, N.Y., sediments, type strain 32 S. algae OK-1 ATCC 51192, type strain, isolated from red algae 51 S. amazonensis SB2B Isolated from Amazon water, type strain 55 S. baltica 63 NCTC 10735, Japan, isolated from oil brine, type strain 60 Shewanella sp. strain MR-4 Isolated from the Black Sea water column, type strain 33 S. pealeana ANG-SQ1 Isolated from accessory nidamental gland of a squid, psychrotolerant, type strain 25 S. frigidimarina ACAM 591 Isolated from Antarctic Sea ice, type strain 7 S. putrefaciens 95 ATCC 8071, isolated from spoiled butter with surface taint, type strain 46 S. woodyi MS32 Isolated from seawater detritus, Alboran Sea, type strain 29 184 ATCC 8073, isolated from spoiled butter with surface taint 46 CL 256/73 NCTC 12093, isolate from human cerebrospinal fluid, type strain 18 CN32 Isolated from anaerobic subsurface core sample, New Mexico 14, 59 Plasmids and vectors pLAFR5 21.5-kb broad-host-range cosmid cloning vector; Tc r , lacZ 22 EZ::TN 1.2-kb transposon used for in vitro mutagenesis Epicentre pSALT1 pLAFR5-based 45-kb As(III) r cosmid from ANA-3 genomic DNA library; contains arsDABC , Tc r , confers resistance to As(III) and As(V) This study pSALT1-B10 As(III)-sensitive pSALT1; arsB :: kan (EZ::TN), Km r Tc r This study pSMV8 9.1-kb mobilizable suicide vector; oriR6K mobRP4 , Gm r D. Lies, Caltech parsB :: kan pSMV8 with Km r Tn-interrupted arsB gene from pSALT1-B10 This study Open in a separate window a Str r , streptomycin resistance; Nal r , nalidixic acid resistance; Tc r , tetracycline resistance; Gm r , gentamicin resistance.
Techniques: Plasmid Preparation, Clone Assay, Conjugation Assay, Isolation, Mutagenesis, Derivative Assay, In Vitro
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