runx2 s 19  (Santa Cruz Biotechnology)

Journal: The Journal of Biological Chemistry

Article Title: Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B *

doi: 10.1074/jbc.M115.657825

Figure Lengend Snippet: TABLE 1

Article Snippet: Primaryantibodies used were the following: αTFIIB C-18 (sc-225, Santa Cruz Biotechnology), RNA-PolII N-20 (sc-899, Santa Cruz Biotechnology), Runx2 S-19 (sc-12488, Santa Cruz Biotechnology), WDR5 (ab56919, Abcam), NO66 3354c5a (sc-81341, Santa Cruz Biotechnology), JARID1B (ab50958, Abcam), EZH2 (39901, Active Motif), UTX/KDM6A (ab91231, Abcam), PRMT5/JBP1 (611539, BD Biosciences), P300 N-15 (sc-584, Santa Cruz Biotechnology).

Techniques: Expressing, Knockdown

Journal: The Journal of Biological Chemistry

Article Title: Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B *

doi: 10.1074/jbc.M115.657825

Figure Lengend Snippet: TABLE 1

Article Snippet: Primaryantibodies used were the following: αTFIIB C-18 (sc-225, Santa Cruz Biotechnology), RNA-PolII N-20 (sc-899, Santa Cruz Biotechnology), Runx2 S-19 (sc-12488, Santa Cruz Biotechnology), WDR5 (ab56919, Abcam), NO66 3354c5a (sc-81341, Santa Cruz Biotechnology), JARID1B (ab50958, Abcam), EZH2 (39901, Active Motif), UTX/KDM6A (ab91231, Abcam), PRMT5/JBP1 (611539, BD Biosciences), P300 N-15 (sc-584, Santa Cruz Biotechnology).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway

doi: 10.1371/journal.pone.0068926

Figure Lengend Snippet: Oligodeoxynucleotide primers used for qRT-PCR.

Article Snippet: Primary α-tubulin antibody was purchased from Millipore (MAB5566, Billerica, MA, USA); anti-β-actin (N-21), anti-HGF (H-170), and anti-Runx2 (S-19) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques:

Journal: PLoS ONE

Article Title: Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway

doi: 10.1371/journal.pone.0068926

Figure Lengend Snippet: (A). hPDL cells were cultured in the presence and absence of daily LIPUS stimulation. BMP-2 mRNA expression was determined using qRT-PCR. (B). BMP-2 protein (ROW 1; 45 kDa) can be detected in the control and LIPUS groups (1: CON; 2: LIPUS). The LIPUS groups showed higher expression of BMP-2 from day 5. (C). Quantification of BMP-2 protein expression in the LIPUS stimulation group was greater than that in the control group on days 5, 7, and 14. *indicates P<0.05 . (D–F). Rat upper first molars were stimulated with or without LIPUS for different time intervals, and HGF, Runx2, BMP-2 were determined by qRT-PCR and Western blot, respectively. The data indicated that LIPUS increased HGF, Runx2, and BMP-2 mRNA (D: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01 ) and protein expression (E, F: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01 ) in vivo . The data are the mean ± SD of three separate experiments.

Article Snippet: Primary α-tubulin antibody was purchased from Millipore (MAB5566, Billerica, MA, USA); anti-β-actin (N-21), anti-HGF (H-170), and anti-Runx2 (S-19) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Control, Western Blot, In Vivo

Journal: PLoS ONE

Article Title: Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway

doi: 10.1371/journal.pone.0068926

Figure Lengend Snippet: (A). hPDL cells were incubated with HGF for 24 h, and BMP-2 mRNA was examined by qRT-PCR. (B and C) hPDL cells were incubated with HGF for 48 h, and BMP-2 protein amounts were detected by Western blotting. The data shows that HGF significantly increased BMP-2 expression. (D). Cells were transfected with Runx2 siRNA for 24 h followed by stimulation with LIPUS for 5 days, and BMP-2 mRNA expression was examined by qRT-PCR. (E and F) hPDL cells were transfected with Runx2 siRNA for 5 days, and BMP-2 protein expression was examined by Western blot. Transfection of cells with Runx2 siRNA reduced LIPUS-increased BMP-2 expression. * P<0.05 (** P<0.01 ) as compared with the control group.

Article Snippet: Primary α-tubulin antibody was purchased from Millipore (MAB5566, Billerica, MA, USA); anti-β-actin (N-21), anti-HGF (H-170), and anti-Runx2 (S-19) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Incubation, Quantitative RT-PCR, Western Blot, Expressing, Transfection, Control