rtcb rna ligase  (New England Biolabs)


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  • 94
    Name:
    RtcB Ligase
    Description:
    RtcB Ligase 25 rxns
    Catalog Number:
    M0458S
    Price:
    72
    Category:
    RNA Ligases
    Size:
    25 rxns
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    Structured Review

    New England Biolabs rtcb rna ligase
    RtcB Ligase
    RtcB Ligase 25 rxns
    https://www.bioz.com/result/rtcb rna ligase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rtcb rna ligase - by Bioz Stars, 2021-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts
    Article Snippet: .. 10 pmol of this purified T4-PNK-treated RNA or of the gel purified RNA was ligated using RtcB Ligase (New England Biolabs M0458) for 1 h at 37 °C. ..

    Article Title: m6A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development
    Article Snippet: .. Total RNA was purified by RNeasy mini kit (QIAGEN) and RNAs with 2’-3’ cyclic phosphate were ligated to the adapter 5’ rGrArUrCrGrUCGGACTGTAGAACTCTGAAC 3’ using RtcB RNA ligase (New England Biolabs). ..

    Article Title: Resurrecting self-cleaving mini-ribozymes from 40-million-year-old LINE-1 elements in human genome
    Article Snippet: .. The transcribed and purified RNAs (10 pmol) of the mutant library were mixed with 100 pmol rM13R_5desBio_3P (Supplementary Table S4), 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP, 2 μl 10mM MnCl2, 0.2 μl of Murine RNase Inhibitor (40 U/μl, New England Biolabs) and 1 μl RtcB RNA ligase (New England Biolabs) to a total volume of 20 μl. ..

    Article Title: Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts
    Article Snippet: .. 10 pmol of this purified T4-PNK-treated RNA or of the gel purified RNA was ligated using RtcB Ligase (New England Biolabs M0458) for 1 h at 37 °C. ..

    Ligation:

    Article Title: Small circRNAs with self-cleaving ribozymes are highly expressed in diverse metazoan transcriptomes
    Article Snippet: .. For the assays of RtcB ligation and self-ligation, 1–10 ng of gel-purified radiolabelled retrozyme RNAs, or 1–4 μg for non-radiolabelled RNAs, were firstly denatured at 95°C for 1 min, cooled down to 25°C (either 0.5°C/s or 1°C/min, with similar results), and then incubated either in the presence of RtcB ligase in its corresponding buffer for 1 h at 37°C (New England Biolabs) or in 50 mM Tris–HCl, pH 8 and 10 to 50 mM MgCl2 for 1 h at 25°C, respectively. ..

    Article Title: Small circRNAs with self-cleaving ribozymes are frequently expressed in metazoan transcriptomes
    Article Snippet: .. For the assays of RtcB ligation, 1 to 10 ng of gel-purified retrozyme RNA were ligated using RtcB ligase in its corresponding buffer for 1 h at 37 °C (New England Biolabs). ..

    Incubation:

    Article Title: Small circRNAs with self-cleaving ribozymes are highly expressed in diverse metazoan transcriptomes
    Article Snippet: .. For the assays of RtcB ligation and self-ligation, 1–10 ng of gel-purified radiolabelled retrozyme RNAs, or 1–4 μg for non-radiolabelled RNAs, were firstly denatured at 95°C for 1 min, cooled down to 25°C (either 0.5°C/s or 1°C/min, with similar results), and then incubated either in the presence of RtcB ligase in its corresponding buffer for 1 h at 37°C (New England Biolabs) or in 50 mM Tris–HCl, pH 8 and 10 to 50 mM MgCl2 for 1 h at 25°C, respectively. ..

    Mutagenesis:

    Article Title: Resurrecting self-cleaving mini-ribozymes from 40-million-year-old LINE-1 elements in human genome
    Article Snippet: .. The transcribed and purified RNAs (10 pmol) of the mutant library were mixed with 100 pmol rM13R_5desBio_3P (Supplementary Table S4), 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP, 2 μl 10mM MnCl2, 0.2 μl of Murine RNase Inhibitor (40 U/μl, New England Biolabs) and 1 μl RtcB RNA ligase (New England Biolabs) to a total volume of 20 μl. ..

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  • 94
    New England Biolabs rtcb rna ligase
    Rtcb Rna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rtcb rna ligase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rtcb rna ligase - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

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