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Illumina Inc rta 1 12 4 2
Rta 1 12 4 2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rta 1 12 4 2/product/Illumina Inc
Average 83 stars, based on 7 article reviews
Price from $9.99 to $1999.99
rta 1 12 4 2 - by Bioz Stars, 2020-01
83/100 stars

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Related Articles

Nucleic Acid Electrophoresis:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: A total of 150 μL of each amplified library (14 - 16 PCR cycles) was size selected (c. 300 - 550 bp) by gel electrophoresis and eluted into 20 μL EB buffer (MinElute Gel Purification Kit, Qiagen, UK.) .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: A total of 150 µL of each amplified library (16–18 PCR cycles, library dependent) was size selected (c. 350–650 bp) by gel electrophoresis . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Multiplex Assay:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: Following heat inactivation individual ligation reactions were then combined in appropriate multiplex pools / libraries (5 parental samples or 15 offspring samples each). .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Following heat inactivation at 65°C for 20 minutes, the ligation reactions were slowly cooled to room temperature (over 1 hour) then combined in appropriate multiplex pools ( ). .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: After heat-inactivation at 65 °C for 20 min, the ligation reactions were slowly cooled to room temperature (over 1 h), then combined in appropriate multiplex pools (see Additional file : Table S1). .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

Amplification:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: A total of 150 μL of each amplified library (14 - 16 PCR cycles) was size selected (c. 300 - 550 bp) by gel electrophoresis and eluted into 20 μL EB buffer (MinElute Gel Purification Kit, Qiagen, UK.) .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: A total of 150 µL of each amplified library (16–18 PCR cycles, library dependent) was size selected (c. 350–650 bp) by gel electrophoresis . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Agarose Gel Electrophoresis:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 – 500 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, as in the original RAD protocol [ ]. .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250–550 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, exactly as in the original RAD protocol , . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 to 550 bp) by agarose gel separation [ ] was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation and library amplification, exactly as in the original RAD protocol [ , ]. .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

Ligation:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 – 500 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, as in the original RAD protocol [ ]. .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250–550 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, exactly as in the original RAD protocol , . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 to 550 bp) by agarose gel separation [ ] was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation and library amplification, exactly as in the original RAD protocol [ , ]. .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

Sequencing:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data). .. (iii) RNA-Seq The sequence data used to generate the RNA-Seq SNP dataset were part of a larger ongoing study with the aim of investigating the transcriptome of Atlantic salmon fry with disparate genetic resistance to the Infectious Pancreatic Necrosis Virus (IPNV).

Article Title: Identification of a Sex-Linked SNP Marker in the Salmon Louse (Lepeophtheirus salmonis) Using RAD Sequencing
Article Snippet: .. Raw sequence data were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina). .. RAD library qualitative and quantitative checks, Illumina sequencing and processing of raw sequence reads were performed at The GenePool Genomics Facility (University of Edinburgh, UK).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Paragraph title: RAD Library Preparation and Sequencing ... Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Paragraph title: RAD library preparation and sequencing ... Raw reads were processed using RTA 1.12.4.2 (Illumina).

Real-time Polymerase Chain Reaction:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: Libraries were accurately quantified by qPCR (Kapa Library), combined as appropriate and run on an Illumina Hiseq 2000. .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Identification of a Sex-Linked SNP Marker in the Salmon Louse (Lepeophtheirus salmonis) Using RAD Sequencing
Article Snippet: The four RAD library pools were further quality checked and quantified by qPCR (KAPA Library) prior to sequencing on one lane of the Illumina HiSeq 2000 platform (v3 chemistry) using 100 bp paired-end reads (EBI Sequence Read Archive (SRA) study ERP002400). .. Raw sequence data were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Libraries were accurately quantified by qPCR (Kapa Library) and run in four lanes of an Illumina HiSeq 2000, using 100 base paired-end reads (v3 chemistry). .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

Polymerase Chain Reaction:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: A total of 150 μL of each amplified library (14 - 16 PCR cycles) was size selected (c. 300 - 550 bp) by gel electrophoresis and eluted into 20 μL EB buffer (MinElute Gel Purification Kit, Qiagen, UK.) .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: A total of 150 µL of each amplified library (16–18 PCR cycles, library dependent) was size selected (c. 350–650 bp) by gel electrophoresis . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Selection:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 – 500 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, as in the original RAD protocol [ ]. .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250–550 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, exactly as in the original RAD protocol , . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 to 550 bp) by agarose gel separation [ ] was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation and library amplification, exactly as in the original RAD protocol [ , ]. .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

High Throughput Screening Assay:

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Following a final gel elution step into 20 µL EB buffer (MinElute Gel Purification Kit, Qiagen), the libraries were sent to The GenePool Genomics Facility at the University of Edinburgh, UK, for quality control and high-throughput sequencing. .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Following a final gel elution step into 20 μL EB buffer (MinElute Gel Purification Kit, Qiagen), 16 libraries (7 animals in the parental library, 12 animals in each of 15 progeny libraries) were sent to the Edinburgh Genomics facility at the University of Edinburgh, UK, for quality control and high-throughput sequencing. .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

Sonication:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 – 500 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, as in the original RAD protocol [ ]. .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250–550 bp) by agarose gel separation was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation, library amplification, exactly as in the original RAD protocol , . .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Shearing (Covaris S2 sonication) and initial size selection (250 to 550 bp) by agarose gel separation [ ] was followed by gel purification, end repair, dA overhang addition, P2 paired-end adapter ligation and library amplification, exactly as in the original RAD protocol [ , ]. .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

Gel Purification:

Article Title: Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)
Article Snippet: A total of 150 μL of each amplified library (14 - 16 PCR cycles) was size selected (c. 300 - 550 bp) by gel electrophoresis and eluted into 20 μL EB buffer (MinElute Gel Purification Kit, Qiagen, UK.) .. Raw reads were processed using RTA 1.12.4.2 and Casava 1.6 (Illumina, USA) and all sequence data were deposited in the ENA under accession numbers PRJEB4783 (paired-end data) and PRJEB4785 (single-end data).

Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Article Snippet: Following a final gel elution step into 20 µL EB buffer (MinElute Gel Purification Kit, Qiagen), the libraries were sent to The GenePool Genomics Facility at the University of Edinburgh, UK, for quality control and high-throughput sequencing. .. Raw reads were process using RTA 1.12.4.2 and Casava 1.6 (Illumina).

Article Title: A new SNP-based vision of the genetics of sex determination in European sea bass (Dicentrarchus labrax)
Article Snippet: Following a final gel elution step into 20 μL EB buffer (MinElute Gel Purification Kit, Qiagen), 16 libraries (7 animals in the parental library, 12 animals in each of 15 progeny libraries) were sent to the Edinburgh Genomics facility at the University of Edinburgh, UK, for quality control and high-throughput sequencing. .. Raw reads were processed using RTA 1.12.4.2 (Illumina).

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    Illumina Inc illumina rta 1 12 4 2
    Illumina Rta 1 12 4 2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina rta 1 12 4 2/product/Illumina Inc
    Average 88 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    illumina rta 1 12 4 2 - by Bioz Stars, 2020-01
    88/100 stars
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