Structured Review

Promega rsa i
Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or <t>Rsa</t> I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder
Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rsa i/product/Promega
Average 92 stars, based on 48 article reviews
Price from $9.99 to $1999.99
rsa i - by Bioz Stars, 2020-10
92/100 stars

Images

1) Product Images from "Characterization of the Bacterial Diversity in Indo-West Pacific Loliginid and Sepiolid Squid Light Organs"

Article Title: Characterization of the Bacterial Diversity in Indo-West Pacific Loliginid and Sepiolid Squid Light Organs

Journal: Microbial ecology

doi: 10.1007/s00248-012-0099-6

Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder
Figure Legend Snippet: Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

Techniques Used:

2) Product Images from "Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA"

Article Title: Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA

Journal: Saudi Journal of Biological Sciences

doi: 10.1016/j.sjbs.2016.02.014

Plasmid map of pCS36-4CPA with restriction sites of the endonucleases Alu I, Kzo 9I and Rsa I. The regions transcribed as RNAI and RNAII are shown as green arrows. The single-strand initiation site ( ssi ) and multimer resolution site ( mrs ) are coloured in pink and yellow, respectively. The mobilisation machinery genes are depicted as red arrows. The open reading frames ORF5, ORF6 and ORF7 are shown as unpainted arrows. Abbreviations: oriV , origin of replication; oriT , origin of transfer; nic , sequence-specific cleavage site.
Figure Legend Snippet: Plasmid map of pCS36-4CPA with restriction sites of the endonucleases Alu I, Kzo 9I and Rsa I. The regions transcribed as RNAI and RNAII are shown as green arrows. The single-strand initiation site ( ssi ) and multimer resolution site ( mrs ) are coloured in pink and yellow, respectively. The mobilisation machinery genes are depicted as red arrows. The open reading frames ORF5, ORF6 and ORF7 are shown as unpainted arrows. Abbreviations: oriV , origin of replication; oriT , origin of transfer; nic , sequence-specific cleavage site.

Techniques Used: Plasmid Preparation, Sequencing

3) Product Images from "Heterogeneity of Leishmania infantum chagasi Kinetoplast DNA in Teresina (Brazil)"

Article Title: Heterogeneity of Leishmania infantum chagasi Kinetoplast DNA in Teresina (Brazil)

Journal: The American Journal of Tropical Medicine and Hygiene

doi: 10.4269/ajtmh.2010.09-0600

kDNA restriction fragment length polymorphisms (RFLPs) of Leishmania infantum chagasi strains digested with either Hpa II or Rsa I, and separated by gel electrophoresis on 3% Metaphor agarose. Samples are in the same lane for both gels. From left to right:
Figure Legend Snippet: kDNA restriction fragment length polymorphisms (RFLPs) of Leishmania infantum chagasi strains digested with either Hpa II or Rsa I, and separated by gel electrophoresis on 3% Metaphor agarose. Samples are in the same lane for both gels. From left to right:

Techniques Used: Nucleic Acid Electrophoresis

4) Product Images from "Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method"

Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method

Journal: Journal of Clinical Microbiology

doi:

Representative diversity revealed by M46 PCR-RFLP in M. catarrhalis . (A) Amplimer B; (B) amplimer C. Samples were treated with Hae III (lanes 1 to 7) or with Rsa I (lanes 8 to 14) or are undigested amplimers (lanes 15 to 21). Each lane contained 10 μl of PCR product. Lanes 1, 8, and 15, sample ATCC 25238; lanes 2, 9, and 16, sample TN16a; lanes 3, 10, and 17, sample BL2; lanes 4, 11, and 18, sample NY1a; lanes 5, 12, and 19, sample TN17a; lanes 6, 13, and 20, sample TN7a; lanes 7, 14, and 21, sample TN13a. Lanes M are 100-to-1,500-bp ladders, with double-bright bands at 600 bp and additional fragments of 2,000 bp.
Figure Legend Snippet: Representative diversity revealed by M46 PCR-RFLP in M. catarrhalis . (A) Amplimer B; (B) amplimer C. Samples were treated with Hae III (lanes 1 to 7) or with Rsa I (lanes 8 to 14) or are undigested amplimers (lanes 15 to 21). Each lane contained 10 μl of PCR product. Lanes 1, 8, and 15, sample ATCC 25238; lanes 2, 9, and 16, sample TN16a; lanes 3, 10, and 17, sample BL2; lanes 4, 11, and 18, sample NY1a; lanes 5, 12, and 19, sample TN17a; lanes 6, 13, and 20, sample TN7a; lanes 7, 14, and 21, sample TN13a. Lanes M are 100-to-1,500-bp ladders, with double-bright bands at 600 bp and additional fragments of 2,000 bp.

Techniques Used: Polymerase Chain Reaction

5) Product Images from "Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere"

Article Title: Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.68.3.1146-1156.2002

Restriction fragment length polymorphism pattern for Rsa I-digested 16S rDNA. The 16S rDNA was amplified, cut as described in Materials and Methods, and separated on gels. The samples were analyzed at the beginning of the storage period (BS), after storage at 4°C, and after storage at 10°C. The different restriction patterns are designated with letters. mw, molecular weight standards.
Figure Legend Snippet: Restriction fragment length polymorphism pattern for Rsa I-digested 16S rDNA. The 16S rDNA was amplified, cut as described in Materials and Methods, and separated on gels. The samples were analyzed at the beginning of the storage period (BS), after storage at 4°C, and after storage at 10°C. The different restriction patterns are designated with letters. mw, molecular weight standards.

Techniques Used: Amplification, Molecular Weight

6) Product Images from "Ochratoxigenic Black Species of Aspergilli in Grape Fruits of Northern Italy Identified by an Improved PCR-RFLP Procedure "

Article Title: Ochratoxigenic Black Species of Aspergilli in Grape Fruits of Northern Italy Identified by an Improved PCR-RFLP Procedure

Journal: Toxins

doi: 10.3390/toxins4020042

Ribosomal DNA restriction patterns exhibited by Aspergillus isolates from grapes after digestion with the restriction endonucleases Hha I, Rsa I and Hinf I. Lane M corresponds to the 50 bp molecular weight marker (Gelpilot, Qiagen).
Figure Legend Snippet: Ribosomal DNA restriction patterns exhibited by Aspergillus isolates from grapes after digestion with the restriction endonucleases Hha I, Rsa I and Hinf I. Lane M corresponds to the 50 bp molecular weight marker (Gelpilot, Qiagen).

Techniques Used: Molecular Weight, Marker

7) Product Images from "Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a"

Article Title: Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

Bisulphite genomic sequencing confirms the presence of non-CpG methylation in ES cell DNA. Sequence of J1–22, a PCR product derived from a bisulphite-modified Rsa I fragment from wild-type ES cells ( Dnmt1 +/+ ) by using primers directed toward the linker DNA. The small arrows indicate the positions of nonsymmetrically methylated cytosine residues. The large solid arrows indicate the modified Eco RI linker DNA sequences. The dashed arrows indicate the sequences to which primers were designed for specific amplification of this DNA segment from bisulphite-modified but unlinked DNA.
Figure Legend Snippet: Bisulphite genomic sequencing confirms the presence of non-CpG methylation in ES cell DNA. Sequence of J1–22, a PCR product derived from a bisulphite-modified Rsa I fragment from wild-type ES cells ( Dnmt1 +/+ ) by using primers directed toward the linker DNA. The small arrows indicate the positions of nonsymmetrically methylated cytosine residues. The large solid arrows indicate the modified Eco RI linker DNA sequences. The dashed arrows indicate the sequences to which primers were designed for specific amplification of this DNA segment from bisulphite-modified but unlinked DNA.

Techniques Used: Genomic Sequencing, CpG Methylation Assay, Sequencing, Polymerase Chain Reaction, Derivative Assay, Modification, Methylation, Amplification

8) Product Images from "Comprehensive Identification of Salmonella enterica Serovar Typhimurium Genes Required for Infection of BALB/c Mice"

Article Title: Comprehensive Identification of Salmonella enterica Serovar Typhimurium Genes Required for Infection of BALB/c Mice

Journal: PLoS Pathogens

doi: 10.1371/journal.ppat.1000529

Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic DNA is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between Rsa I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.
Figure Legend Snippet: Diagrammatic representation of the TMDH process. (A) A library of transposon mutants is obtained using a custom transposon with outward-facing T7 and SP6 promoters. Genomic DNA is extracted from the library and digested using a restriction endonuclease ( Rsa I). Labelled RNA run-offs are obtained from the T7 and SP6 promoters by in vitro transcription. (B) The labelled RNA run-offs are hybridised to genome-wide tiling microarrays. By examining the distribution of microarray signals between Rsa I restriction sites (vertical black lines) it is possible to infer the location of the transposon (green triangle). Comparison of the library grown in vitro (input) with the library obtained after passage through a mouse (output) allows attenuating mutants to be identified, since they give lower signals in the output.

Techniques Used: In Vitro, Genome Wide, Microarray

9) Product Images from "Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere"

Article Title: Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.68.3.1146-1156.2002

Restriction fragment length polymorphism pattern for Rsa I-digested 16S rDNA. The 16S rDNA was amplified, cut as described in Materials and Methods, and separated on gels. The samples were analyzed at the beginning of the storage period (BS), after storage at 4°C, and after storage at 10°C. The different restriction patterns are designated with letters. mw, molecular weight standards.
Figure Legend Snippet: Restriction fragment length polymorphism pattern for Rsa I-digested 16S rDNA. The 16S rDNA was amplified, cut as described in Materials and Methods, and separated on gels. The samples were analyzed at the beginning of the storage period (BS), after storage at 4°C, and after storage at 10°C. The different restriction patterns are designated with letters. mw, molecular weight standards.

Techniques Used: Amplification, Molecular Weight

10) Product Images from "Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA"

Article Title: Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA

Journal: Saudi Journal of Biological Sciences

doi: 10.1016/j.sjbs.2016.02.014

Plasmid map of pCS36-4CPA with restriction sites of the endonucleases Alu I, Kzo 9I and Rsa I. The regions transcribed as RNAI and RNAII are shown as green arrows. The single-strand initiation site ( ssi ) and multimer resolution site ( mrs ) are coloured in pink and yellow, respectively. The mobilisation machinery genes are depicted as red arrows. The open reading frames ORF5, ORF6 and ORF7 are shown as unpainted arrows. Abbreviations: oriV , origin of replication; oriT , origin of transfer; nic , sequence-specific cleavage site.
Figure Legend Snippet: Plasmid map of pCS36-4CPA with restriction sites of the endonucleases Alu I, Kzo 9I and Rsa I. The regions transcribed as RNAI and RNAII are shown as green arrows. The single-strand initiation site ( ssi ) and multimer resolution site ( mrs ) are coloured in pink and yellow, respectively. The mobilisation machinery genes are depicted as red arrows. The open reading frames ORF5, ORF6 and ORF7 are shown as unpainted arrows. Abbreviations: oriV , origin of replication; oriT , origin of transfer; nic , sequence-specific cleavage site.

Techniques Used: Plasmid Preparation, Sequencing

Related Articles

Polymerase Chain Reaction:

Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
Article Snippet: .. To detect RFLP variation within PCR-amplified regions encompassed by M46 DNA, 10-μl aliquots of PCR products were subjected to separate restriction enzyme digestion with Hae III and Rsa I (Promega Corp.) according to the manufacturer’s instructions. ..

Article Title: Ochratoxigenic Black Species of Aspergilli in Grape Fruits of Northern Italy Identified by an Improved PCR-RFLP Procedure
Article Snippet: .. The reaction mixtures were performed in a Thermocycler (Biometra, Germany) with 35 cycles consisting of 1 min at 95 °C, 1 min at 52 °C and 2 min at 72 °C and the final extension time with 10 min. Then, the PCR products were purified by using Qiagen (Valencia, CA, USA) PCR purification kit and digested with the restriction enzymes Hha I, Hinf I and Rsa I (Promega, Madison, WI, USA). .. Each 20 μL reaction mixture contained 2 μL of 10X reaction buffer, 0.2 μL of BSA (10 μg μL−1 ), 0.5 μL of restriction enzyme (10 U μL−1 ), 5 μL of purified DNA and 12.3 μL of dH2 O and incubated at 37 °C for 2 h. The restriction fragments were separated on 2% agarose gel at 50 V, stained SYBR-safe (Invitrogen) and photographed using a Gel Doc 1000 system.

Clone Assay:

Article Title: Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA
Article Snippet: .. Later, Kzo 9I, Rsa I and Alu I fragments were cloned into pGEM-3Zf(+) (Promega). .. The recombinant plasmid DNA was then transformed into Escherichia coli DH5α and isolated with a Wizard MaxiPrep kit (Promega).

Amplification:

Article Title: Development and Evaluation of a 16S Ribosomal DNA Array-Based Approach for Describing Complex Microbial Communities in Ready-To-Eat Vegetable Salads Packed in a Modified Atmosphere
Article Snippet: .. Subsequently, the amplification products were cut with Rsa I (GT ↓ AC) in a 21-μl reaction volume containing 1× Buffer C (Promega), 1 μl of Rsa I (Promega), and 5 μl of template from the nested amplification. ..

In Vitro:

Article Title: Comprehensive Identification of Salmonella enterica Serovar Typhimurium Genes Required for Infection of BALB/c Mice
Article Snippet: .. Restriction digests and in vitro transcription 10 µg of genomic DNA from each TMDH input and output pool, and from the untransposed control DNA, was digested using Rsa I (Promega) overnight at 37°C. .. Digested DNA was cleaned using a Qiagen QIAquick PCR Purification Kit and eluted in 30 µl RNase-free water (Qiagen).

Purification:

Article Title: Ochratoxigenic Black Species of Aspergilli in Grape Fruits of Northern Italy Identified by an Improved PCR-RFLP Procedure
Article Snippet: .. The reaction mixtures were performed in a Thermocycler (Biometra, Germany) with 35 cycles consisting of 1 min at 95 °C, 1 min at 52 °C and 2 min at 72 °C and the final extension time with 10 min. Then, the PCR products were purified by using Qiagen (Valencia, CA, USA) PCR purification kit and digested with the restriction enzymes Hha I, Hinf I and Rsa I (Promega, Madison, WI, USA). .. Each 20 μL reaction mixture contained 2 μL of 10X reaction buffer, 0.2 μL of BSA (10 μg μL−1 ), 0.5 μL of restriction enzyme (10 U μL−1 ), 5 μL of purified DNA and 12.3 μL of dH2 O and incubated at 37 °C for 2 h. The restriction fragments were separated on 2% agarose gel at 50 V, stained SYBR-safe (Invitrogen) and photographed using a Gel Doc 1000 system.

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    Promega rsa i
    Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or <t>Rsa</t> I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder
    Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa i/product/Promega
    Average 92 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    rsa i - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    85
    Promega endonuclease rsa i
    The kDNA <t>PCR</t> and RFLP products of the 12 Palestinian L. tropica strains. a , The kDNA PCR products of three of the 12 Palestinian strains: LRC-L890, -L891, -L887, and reference strains of L. tropica , -L758, and L. major , -L137; MW: molecular weight marker ΦX174 DNA/Hinf; b , The RFLP patterns of the six different genotypes resulting from digestion of kDNA PCR products with Rsa I. MW: molecular weight marker ΦX174 DNA/HaeIII Markers; c , Dendrogram based on the kDNA restriction fragment length polymorphism (RFLP) of the strains of L. tropica after digestion of their PCR products, separately, with the endonucleases <t>Rsa</t> I and Mbo I, which yielded ten sub-genotypes that segregated into the Clade A, with kDNA sub-type Ltro -kD1 and the Clade B, with kDNA sub-type Ltro -kD2. It was constructed using RAPDistance Package (version 1.04) and was based on the presence or absence of the bands in each sample. The numbers on the branches represent the % of difference. The lengths of the branches indicate the degree of similarity in fingerprints among the strains of L. tropica relative to the strain of L. major , MHOM/IL/67/JerichoII (=LRC-L137), which served as an out-group in this analysis: Ts = Tubas; Ma = Meselya; Yn = El-Yamoon; Jn = Jenin; S-h = Silat El hartheyia; Tb = Tiberias; KA = Kfar Adumim. The zymodemal designations and EF serotypes are given for complete comparison.
    Endonuclease Rsa I, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonuclease rsa i/product/Promega
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    endonuclease rsa i - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

    Journal: Microbial ecology

    Article Title: Characterization of the Bacterial Diversity in Indo-West Pacific Loliginid and Sepiolid Squid Light Organs

    doi: 10.1007/s00248-012-0099-6

    Figure Lengend Snippet: Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

    Article Snippet: RFLP analysis was completed as described by Urakawa et al. [ , ] using three restriction endonucleases: Rsa I (5′GTAC3′), Hha I (5′GCGC3′) and Dde I (5′CTNAG3′; Promega Corporation, Madison, WI).

    Techniques:

    Plasmid map of pCS36-4CPA with restriction sites of the endonucleases Alu I, Kzo 9I and Rsa I. The regions transcribed as RNAI and RNAII are shown as green arrows. The single-strand initiation site ( ssi ) and multimer resolution site ( mrs ) are coloured in pink and yellow, respectively. The mobilisation machinery genes are depicted as red arrows. The open reading frames ORF5, ORF6 and ORF7 are shown as unpainted arrows. Abbreviations: oriV , origin of replication; oriT , origin of transfer; nic , sequence-specific cleavage site.

    Journal: Saudi Journal of Biological Sciences

    Article Title: Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA

    doi: 10.1016/j.sjbs.2016.02.014

    Figure Lengend Snippet: Plasmid map of pCS36-4CPA with restriction sites of the endonucleases Alu I, Kzo 9I and Rsa I. The regions transcribed as RNAI and RNAII are shown as green arrows. The single-strand initiation site ( ssi ) and multimer resolution site ( mrs ) are coloured in pink and yellow, respectively. The mobilisation machinery genes are depicted as red arrows. The open reading frames ORF5, ORF6 and ORF7 are shown as unpainted arrows. Abbreviations: oriV , origin of replication; oriT , origin of transfer; nic , sequence-specific cleavage site.

    Article Snippet: Later, Kzo 9I, Rsa I and Alu I fragments were cloned into pGEM-3Zf(+) (Promega).

    Techniques: Plasmid Preparation, Sequencing

    kDNA restriction fragment length polymorphisms (RFLPs) of Leishmania infantum chagasi strains digested with either Hpa II or Rsa I, and separated by gel electrophoresis on 3% Metaphor agarose. Samples are in the same lane for both gels. From left to right:

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Heterogeneity of Leishmania infantum chagasi Kinetoplast DNA in Teresina (Brazil)

    doi: 10.4269/ajtmh.2010.09-0600

    Figure Lengend Snippet: kDNA restriction fragment length polymorphisms (RFLPs) of Leishmania infantum chagasi strains digested with either Hpa II or Rsa I, and separated by gel electrophoresis on 3% Metaphor agarose. Samples are in the same lane for both gels. From left to right:

    Article Snippet: After the procedure, pellets were rinsed in 10 μL of water and 5 μL were used for endonuclease digestion for 2 h using either Rsa I or Hpa II in enzyme buffer (Promega, Madison, WI), according to the manufacturer's recommendations.

    Techniques: Nucleic Acid Electrophoresis

    The kDNA PCR and RFLP products of the 12 Palestinian L. tropica strains. a , The kDNA PCR products of three of the 12 Palestinian strains: LRC-L890, -L891, -L887, and reference strains of L. tropica , -L758, and L. major , -L137; MW: molecular weight marker ΦX174 DNA/Hinf; b , The RFLP patterns of the six different genotypes resulting from digestion of kDNA PCR products with Rsa I. MW: molecular weight marker ΦX174 DNA/HaeIII Markers; c , Dendrogram based on the kDNA restriction fragment length polymorphism (RFLP) of the strains of L. tropica after digestion of their PCR products, separately, with the endonucleases Rsa I and Mbo I, which yielded ten sub-genotypes that segregated into the Clade A, with kDNA sub-type Ltro -kD1 and the Clade B, with kDNA sub-type Ltro -kD2. It was constructed using RAPDistance Package (version 1.04) and was based on the presence or absence of the bands in each sample. The numbers on the branches represent the % of difference. The lengths of the branches indicate the degree of similarity in fingerprints among the strains of L. tropica relative to the strain of L. major , MHOM/IL/67/JerichoII (=LRC-L137), which served as an out-group in this analysis: Ts = Tubas; Ma = Meselya; Yn = El-Yamoon; Jn = Jenin; S-h = Silat El hartheyia; Tb = Tiberias; KA = Kfar Adumim. The zymodemal designations and EF serotypes are given for complete comparison.

    Journal: Parasites & Vectors

    Article Title: Genetic, serological and biochemical characterization of Leishmania tropica from foci in northern Palestine and discovery of zymodeme MON-307

    doi: 10.1186/1756-3305-5-121

    Figure Lengend Snippet: The kDNA PCR and RFLP products of the 12 Palestinian L. tropica strains. a , The kDNA PCR products of three of the 12 Palestinian strains: LRC-L890, -L891, -L887, and reference strains of L. tropica , -L758, and L. major , -L137; MW: molecular weight marker ΦX174 DNA/Hinf; b , The RFLP patterns of the six different genotypes resulting from digestion of kDNA PCR products with Rsa I. MW: molecular weight marker ΦX174 DNA/HaeIII Markers; c , Dendrogram based on the kDNA restriction fragment length polymorphism (RFLP) of the strains of L. tropica after digestion of their PCR products, separately, with the endonucleases Rsa I and Mbo I, which yielded ten sub-genotypes that segregated into the Clade A, with kDNA sub-type Ltro -kD1 and the Clade B, with kDNA sub-type Ltro -kD2. It was constructed using RAPDistance Package (version 1.04) and was based on the presence or absence of the bands in each sample. The numbers on the branches represent the % of difference. The lengths of the branches indicate the degree of similarity in fingerprints among the strains of L. tropica relative to the strain of L. major , MHOM/IL/67/JerichoII (=LRC-L137), which served as an out-group in this analysis: Ts = Tubas; Ma = Meselya; Yn = El-Yamoon; Jn = Jenin; S-h = Silat El hartheyia; Tb = Tiberias; KA = Kfar Adumim. The zymodemal designations and EF serotypes are given for complete comparison.

    Article Snippet: The PCR product was digested separately with either the endonuclease Rsa I or Mbo I (Promega, Madison, WI), according to the manufacturer’s instructions. kDNA of L. tropica ISER/IL/98/LRC-L758 was included as a positive reference and that of L. major MHOM/IL/67/JerichoII (=LRC-L137) as the out-group. kDNA-RFLP results were analyzed using the RAPDistance Package version 1.04 ( http://www.anu.edu.au/Bozo/software/ ).

    Techniques: Polymerase Chain Reaction, Molecular Weight, Marker, Construct