rq 1 rnase free dnase i  (Promega)

 
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  • 99
    Name:
    RQ1 RNase Free DNase
    Description:
    RQ1 RNA Qualified RNase Free DNase is a DNase I that degrades both dsDNA and ssDNA
    Catalog Number:
    m6101
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Cloning DNA Markers Molecular Biology Enzymes and Reagents
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    Structured Review

    Promega rq 1 rnase free dnase i
    RQ1 RNA Qualified RNase Free DNase is a DNase I that degrades both dsDNA and ssDNA
    https://www.bioz.com/result/rq 1 rnase free dnase i/product/Promega
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rq 1 rnase free dnase i - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: In vivo and in vitro interaction between human transcription factor MOK2 and nuclear lamin A/C
    Article Snippet: .. In brief, transfected HeLa cells were sequentially extracted with: 1% Triton X-100 in TMS buffer (50 mM Tris–HCl pH 7.4, 5 mM MgSO4 , 250 mM sucrose) for 5 min at RT (fraction 1); 50 U/ml RNase-free DNase RQ1 (Promega) in TMS at 37°C for 30 min and adding ammonium sulfate to a final concentration of 0.25 M (fraction 2); 2 M NaCl in TM buffer (10 mM Tris–HCl pH 7.4, 0.02 mM MgSO4 ) twice for 15 min at RT (fraction 3); 50 µg/ml RNase A in TM for 15 min at RT (fraction 4). ..

    Transfection:

    Article Title: Nonencapsidated 5′ Copy-Back Defective Interfering Genomes Produced by Recombinant Measles Viruses Are Recognized by RIG-I and LGP2 but Not MDA5
    Article Snippet: .. Total RNAs extracted from HEK293-T7 and HEK293-T7-NP cells transfected with p2RZ vector in the presence or absence of pEMC-La (a kind gift of M. A. Billeter) were treated with RQ1 RNase-free DNase (Promega). .. Expression levels of IFN-β or ISG (Mx1 and DHX58) were quantified by one-step real-time PCR, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression as an internal control.

    Article Title: In vivo and in vitro interaction between human transcription factor MOK2 and nuclear lamin A/C
    Article Snippet: .. In brief, transfected HeLa cells were sequentially extracted with: 1% Triton X-100 in TMS buffer (50 mM Tris–HCl pH 7.4, 5 mM MgSO4 , 250 mM sucrose) for 5 min at RT (fraction 1); 50 U/ml RNase-free DNase RQ1 (Promega) in TMS at 37°C for 30 min and adding ammonium sulfate to a final concentration of 0.25 M (fraction 2); 2 M NaCl in TM buffer (10 mM Tris–HCl pH 7.4, 0.02 mM MgSO4 ) twice for 15 min at RT (fraction 3); 50 µg/ml RNase A in TM for 15 min at RT (fraction 4). ..

    Plasmid Preparation:

    Article Title: Nonencapsidated 5′ Copy-Back Defective Interfering Genomes Produced by Recombinant Measles Viruses Are Recognized by RIG-I and LGP2 but Not MDA5
    Article Snippet: .. Total RNAs extracted from HEK293-T7 and HEK293-T7-NP cells transfected with p2RZ vector in the presence or absence of pEMC-La (a kind gift of M. A. Billeter) were treated with RQ1 RNase-free DNase (Promega). .. Expression levels of IFN-β or ISG (Mx1 and DHX58) were quantified by one-step real-time PCR, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression as an internal control.

    Hybridization:

    Article Title: snRNA-specific role of SMN in trypanosome snRNP biogenesis in vivo
    Article Snippet: .. Optional RQ1-DNase treatment was done, using 6 U of RQ1 RNase-free DNase (Promega) for 1 h at 37°C to exclude the possibility of hybridization with DNA. .. The fixed and permeabilized cells were washed with PBS and the slides were prehybridized with hybridization buffer (60% deionized formamide, 50 mM sodium phosphate pH 7.2, 0.5 mg/ml salmon sperm DNA, 1 µg/µl yeast tRNA and 5x Denhardt's solution in 2x SSC) for 3–4 h at 72°C in a humid chamber.

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  • 99
    Promega rq 1 rnase free dnase i
    Rq 1 Rnase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq 1 rnase free dnase i/product/Promega
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rq 1 rnase free dnase i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Promega dnase i
    Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Promega
    Average 92 stars, based on 560 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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