rptecs  (Qiagen)


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    Structured Review

    Qiagen rptecs
    Rptecs, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rptecs/product/Qiagen
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rptecs - by Bioz Stars, 2020-04
    94/100 stars

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    Polymerase Chain Reaction:

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. To detect the expressions of Bax, Bcl-2, IκBα, and p65 in RPTECs, the Power SYBR-Green Master Mix Assay (Applied Biosystems) was used following the manufacturer's protocol.

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: Paragraph title: Quantitative reverse transcriptase PCR (qRT-PCR) ... The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA).

    Isolation:

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. To detect the expressions of Bax, Bcl-2, IκBα, and p65 in RPTECs, the Power SYBR-Green Master Mix Assay (Applied Biosystems) was used following the manufacturer's protocol.

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: .. The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. To detect the expressions of Bax, Bcl-2, IκBα, and p65 in RPTECs, the Power SYBR-Green Master Mix Assay (Applied Biosystems) was used following the manufacturer's protocol.

    SYBR Green Assay:

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: Quantitative reverse transcriptase PCR (qRT-PCR) The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. To detect the expressions of Bax, Bcl-2, IκBα, and p65 in RPTECs, the Power SYBR-Green Master Mix Assay (Applied Biosystems) was used following the manufacturer's protocol.

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. To detect the expressions of Bax, Bcl-2, IκBα, and p65 in RPTECs, the Power SYBR-Green Master Mix Assay (Applied Biosystems) was used following the manufacturer's protocol.

    Quantitative RT-PCR:

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. To detect the expressions of Bax, Bcl-2, IκBα, and p65 in RPTECs, the Power SYBR-Green Master Mix Assay (Applied Biosystems) was used following the manufacturer's protocol.

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: Paragraph title: Quantitative reverse transcriptase PCR (qRT-PCR) ... The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA).

    Software:

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: Quantitative reverse transcriptase PCR (qRT-PCR) The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. The data from the qRT-PCR were analyzed by CFX manager software (Bio-Rad Laboratories).

    Article Title: Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
    Article Snippet: The total RNA in RPTECs after relevant treatment was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was reversely transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). .. The data from the qRT-PCR were analyzed by CFX manager software (Bio-Rad Laboratories).

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    Qiagen rptec tert1 cells
    Effect of Cd +2 on <t>RPTEC/TERT1</t> cells. (A). Effect of Cd +2 on the viability RPTEC/TERT1 cells exposed to 9, 27 and 45 μM Cd +2 for 24, 36, 48 and 60 h. Viability was determined by counting the DAPI stained nuclei and is expressed as percent of control. (B). LDH release by Cd +2 treated cells. RPTEC/TERT1 cells were exposed to 45μM Cd +2 for 48 and 60 h. The release of LDH by the cells was quantified spectrophotometrically at a wavelength of 490 nm and is expressed as percent of total LDH release. (C). Caspase-3 activation in Cd +2 treated cells. RPTEC/TERT1 cells were exposed to 9, 18, 27, 36 or 45 μM Cd +2 for 8, 12, 24 and 36 h. Caspase-3 activity was determined by the release of the chromophore, p-nitroanilide, which was quantitated spectrophotometrically at a wavelength of 405 nm. (D). DAPI staining of RPTEC/TERT1 cells exposed to 45 μM Cd +2 for 48 h. **** indicates significantly different in Cd +2 exposed cells when compared to untreated controls at p-value of ≤ 0.01. *** indicates significantly different in Cd +2 exposed cells when compared to untreated controls at p-value of ≤ 0.05.
    Rptec Tert1 Cells, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rptec
    Downregulation of HSPB7 in <t>RCC.</t> qPCR analysis shows that HSPB7 mRNA expression was significantly downregulated (A) in 11 (85%) of 13 RCC tissues compared with the normal renal tissue, and (B) in all the five RCC cell lines compared with normal HEK 293 and <t>RPTEC</t> cells. T and N represent RCC tissue sample and normal renal tissue, respectively. B2M (β2 microglobulin) was used for normalization of expression levels. Values are expressed as the mean ± SD. (C) IHC analysis of a tissue array consisting of 11 pairs of human RCC sample reveals that the expression of HSPB7 was significantly higher in normal kidney tissues than in RCC tissues. According to the intensity of HSPB7 staining, these samples were evaluated as: negative (−), weakly positive (+), moderate positive (++), and strong positive (+++). HSPB7 negative or weakly positive (−/+) were considered low expression, and moderate or strong positive were considered high expression (++/+++). Summary of the IHC results is shown in Table I .
    Rptec, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    86
    Qiagen freeze thawed seven day old rptec culture supernatant
    Downregulation of HSPB7 in <t>RCC.</t> qPCR analysis shows that HSPB7 mRNA expression was significantly downregulated (A) in 11 (85%) of 13 RCC tissues compared with the normal renal tissue, and (B) in all the five RCC cell lines compared with normal HEK 293 and <t>RPTEC</t> cells. T and N represent RCC tissue sample and normal renal tissue, respectively. B2M (β2 microglobulin) was used for normalization of expression levels. Values are expressed as the mean ± SD. (C) IHC analysis of a tissue array consisting of 11 pairs of human RCC sample reveals that the expression of HSPB7 was significantly higher in normal kidney tissues than in RCC tissues. According to the intensity of HSPB7 staining, these samples were evaluated as: negative (−), weakly positive (+), moderate positive (++), and strong positive (+++). HSPB7 negative or weakly positive (−/+) were considered low expression, and moderate or strong positive were considered high expression (++/+++). Summary of the IHC results is shown in Table I .
    Freeze Thawed Seven Day Old Rptec Culture Supernatant, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/freeze thawed seven day old rptec culture supernatant/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    freeze thawed seven day old rptec culture supernatant - by Bioz Stars, 2020-04
    86/100 stars
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    Image Search Results


    Effect of Cd +2 on RPTEC/TERT1 cells. (A). Effect of Cd +2 on the viability RPTEC/TERT1 cells exposed to 9, 27 and 45 μM Cd +2 for 24, 36, 48 and 60 h. Viability was determined by counting the DAPI stained nuclei and is expressed as percent of control. (B). LDH release by Cd +2 treated cells. RPTEC/TERT1 cells were exposed to 45μM Cd +2 for 48 and 60 h. The release of LDH by the cells was quantified spectrophotometrically at a wavelength of 490 nm and is expressed as percent of total LDH release. (C). Caspase-3 activation in Cd +2 treated cells. RPTEC/TERT1 cells were exposed to 9, 18, 27, 36 or 45 μM Cd +2 for 8, 12, 24 and 36 h. Caspase-3 activity was determined by the release of the chromophore, p-nitroanilide, which was quantitated spectrophotometrically at a wavelength of 405 nm. (D). DAPI staining of RPTEC/TERT1 cells exposed to 45 μM Cd +2 for 48 h. **** indicates significantly different in Cd +2 exposed cells when compared to untreated controls at p-value of ≤ 0.01. *** indicates significantly different in Cd +2 exposed cells when compared to untreated controls at p-value of ≤ 0.05.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Effect of Cd +2 on RPTEC/TERT1 cells. (A). Effect of Cd +2 on the viability RPTEC/TERT1 cells exposed to 9, 27 and 45 μM Cd +2 for 24, 36, 48 and 60 h. Viability was determined by counting the DAPI stained nuclei and is expressed as percent of control. (B). LDH release by Cd +2 treated cells. RPTEC/TERT1 cells were exposed to 45μM Cd +2 for 48 and 60 h. The release of LDH by the cells was quantified spectrophotometrically at a wavelength of 490 nm and is expressed as percent of total LDH release. (C). Caspase-3 activation in Cd +2 treated cells. RPTEC/TERT1 cells were exposed to 9, 18, 27, 36 or 45 μM Cd +2 for 8, 12, 24 and 36 h. Caspase-3 activity was determined by the release of the chromophore, p-nitroanilide, which was quantitated spectrophotometrically at a wavelength of 405 nm. (D). DAPI staining of RPTEC/TERT1 cells exposed to 45 μM Cd +2 for 48 h. **** indicates significantly different in Cd +2 exposed cells when compared to untreated controls at p-value of ≤ 0.01. *** indicates significantly different in Cd +2 exposed cells when compared to untreated controls at p-value of ≤ 0.05.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques: Staining, Activation Assay, Activity Assay

    Expression of cadherins in cultures of human proximal tubule cells. (A). Expression of E-cadherin in HK-2, HPT and RPTEC/TERT1 cells. (B). Expression of N-cadherin in HK-2, HPT and RPTEC/TERT1 cells. (C). Expression of P-cadherin in HK-2, HPT and RPTEC/TERT1 cells. (D). Expression of Ksp-cadherin in HK-2, HPT and RPTEC/TERT1 cells. The top graph in figures A–D represent PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Expression of cadherins in cultures of human proximal tubule cells. (A). Expression of E-cadherin in HK-2, HPT and RPTEC/TERT1 cells. (B). Expression of N-cadherin in HK-2, HPT and RPTEC/TERT1 cells. (C). Expression of P-cadherin in HK-2, HPT and RPTEC/TERT1 cells. (D). Expression of Ksp-cadherin in HK-2, HPT and RPTEC/TERT1 cells. The top graph in figures A–D represent PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques: Expressing, Polymerase Chain Reaction, Western Blot

    Expression of tight junction proteins in cultures of human proximal tubule cells. (A). Expression of occludin (OCDN) in HK-2, HPT and RPTEC/TERT1 cells. (B). Expression of claudin 1 (CLDN1) in HK-2, HPT and RPTEC/TERT1 cells. (C). Expression of claudin 4 (CLDN4) in HK-2, HPT and RPTEC/TERT1 cells. The top graph in figures A–C represent PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01. *** indicates significantly different in HPT cells when compared to HK-2 cells at p-value of ≤ 0.05. # indicates significantly different in the RPTEC/TERT1 cells when compared to the HK-2 cells at p-value of ≤ 0.01.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Expression of tight junction proteins in cultures of human proximal tubule cells. (A). Expression of occludin (OCDN) in HK-2, HPT and RPTEC/TERT1 cells. (B). Expression of claudin 1 (CLDN1) in HK-2, HPT and RPTEC/TERT1 cells. (C). Expression of claudin 4 (CLDN4) in HK-2, HPT and RPTEC/TERT1 cells. The top graph in figures A–C represent PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01. *** indicates significantly different in HPT cells when compared to HK-2 cells at p-value of ≤ 0.05. # indicates significantly different in the RPTEC/TERT1 cells when compared to the HK-2 cells at p-value of ≤ 0.01.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques: Expressing, Polymerase Chain Reaction, Western Blot

    Flow cytometry analysis of CD24 and CD133 populations in proximal tubule cells. Analysis of CD24 and CD133 and CD24/CD133 populations in HK-2 (A), HPT (B) and RPTEC/TERT1 (C) cell cultures. (D). Graphical representation of the number of CD24/CD133 co-expressing cells in the three groups of cell cultures. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01. # indicates significantly different in the RPTEC/TERT1 cells when compared to the HPT cells at p-value of ≤ 0.01.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Flow cytometry analysis of CD24 and CD133 populations in proximal tubule cells. Analysis of CD24 and CD133 and CD24/CD133 populations in HK-2 (A), HPT (B) and RPTEC/TERT1 (C) cell cultures. (D). Graphical representation of the number of CD24/CD133 co-expressing cells in the three groups of cell cultures. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01. # indicates significantly different in the RPTEC/TERT1 cells when compared to the HPT cells at p-value of ≤ 0.01.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques: Flow Cytometry, Cytometry, Expressing

    Hierarchical clustering of various proximal tubule cell transcriptomes. All transcript clusters from the HTA 2.0 array (67,528) were subjected to hierarchical clustering using the UPGMA algorithm based on Pearson correlation metric without any statistical filtering of transcript clusters. Samples include triplicate RNA samples from three parallel cultures of HK-2 (HK-2-A, -B, and -C), RPTEC/TERT1 (RPTEC/TERT1-A, -B, and -C) and single RNA samples from six independent isolates of human proximal tubule cells (HPT-1, -2, -3, -4, -5 and -6).

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Hierarchical clustering of various proximal tubule cell transcriptomes. All transcript clusters from the HTA 2.0 array (67,528) were subjected to hierarchical clustering using the UPGMA algorithm based on Pearson correlation metric without any statistical filtering of transcript clusters. Samples include triplicate RNA samples from three parallel cultures of HK-2 (HK-2-A, -B, and -C), RPTEC/TERT1 (RPTEC/TERT1-A, -B, and -C) and single RNA samples from six independent isolates of human proximal tubule cells (HPT-1, -2, -3, -4, -5 and -6).

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques:

    Principal component analysis using all 67,528 non-control transcript clusters. Turquoise dots represents HK-2 cells; grey dots represents HPT isolates, and pink dot represents RPTEC/TERT1 cells. The two outliers of the HPT group are HPT-4 and HPT-5. The X-component accounts for 73% of the variance between groups, and the Y-component represents 26.7% variance between groups.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Principal component analysis using all 67,528 non-control transcript clusters. Turquoise dots represents HK-2 cells; grey dots represents HPT isolates, and pink dot represents RPTEC/TERT1 cells. The two outliers of the HPT group are HPT-4 and HPT-5. The X-component accounts for 73% of the variance between groups, and the Y-component represents 26.7% variance between groups.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques:

    Venn diagram showing differentially expressed genes in HK-2 cells and RPTEC/TERT1 cells in comparison to mortal human proximal tubule cell (HPT) isolates. Pairwise analysis was used to compare the number of differentially expressed genes from HK-2 in comparison to six isolates of HPTs or RPTEC/TERT1 cells in comparison to HPT cells. A differentially expressed gene is defined as one that is an absolute fold change ≥ 2 or ≤ 0.5, and an FDR-adjusted ANOVA and T-test p-value of ≤ 0.05 or ≤ 0.01. (A). Differentially expressed genes in HK-2 vs HPT cells at p ≤ 0.05. (B). Differentially expressed genes in RPTEC/TERT1 vs HPT cells at p ≤ 0.05. (C) Differentially expressed genes in HK-2 vs HPT cells at p ≤ 0.01. (D). Differentially expressed genes in RPTEC/TERT1 vs HPT cells at p ≤ 0.01.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Venn diagram showing differentially expressed genes in HK-2 cells and RPTEC/TERT1 cells in comparison to mortal human proximal tubule cell (HPT) isolates. Pairwise analysis was used to compare the number of differentially expressed genes from HK-2 in comparison to six isolates of HPTs or RPTEC/TERT1 cells in comparison to HPT cells. A differentially expressed gene is defined as one that is an absolute fold change ≥ 2 or ≤ 0.5, and an FDR-adjusted ANOVA and T-test p-value of ≤ 0.05 or ≤ 0.01. (A). Differentially expressed genes in HK-2 vs HPT cells at p ≤ 0.05. (B). Differentially expressed genes in RPTEC/TERT1 vs HPT cells at p ≤ 0.05. (C) Differentially expressed genes in HK-2 vs HPT cells at p ≤ 0.01. (D). Differentially expressed genes in RPTEC/TERT1 vs HPT cells at p ≤ 0.01.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques:

    Transposed principal component analysis after performing discriminant analysis of predefined groups. Discriminant Principal Component Analysis, selecting for the top 0.015% of discriminating genes that discriminates between groups with 100% accuracy following sample-based clustering by UPGMA. Turquoise dots represents HK-2 cells; grey dots represent HPT isolates, and pink dots represent RPTEC/TERT1 cells.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Transposed principal component analysis after performing discriminant analysis of predefined groups. Discriminant Principal Component Analysis, selecting for the top 0.015% of discriminating genes that discriminates between groups with 100% accuracy following sample-based clustering by UPGMA. Turquoise dots represents HK-2 cells; grey dots represent HPT isolates, and pink dots represent RPTEC/TERT1 cells.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques:

    Expression of stem cell markers in cultures of human proximal tubule cells. (A). Expression of CD133 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. (B). Expression of CD24 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. (C). Expression of CD44 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. (D). Expression of ALDH1A1 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. The top graph in figures A–D represent PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01. *** indicates significantly different in HPT cells when compared to HK-2 cells at p-value of ≤ 0.05. # indicates significantly different in the RPTEC/TERT cells when compared to the HPT cells at p-value of ≤ 0.01.

    Journal: Toxicology and applied pharmacology

    Article Title: Human renal tubular cells contain CD24/CD133 progenitor cell populations: implications for tubular regeneration after toxicant induced damage using cadmium as a model

    doi: 10.1016/j.taap.2017.05.038

    Figure Lengend Snippet: Expression of stem cell markers in cultures of human proximal tubule cells. (A). Expression of CD133 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. (B). Expression of CD24 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. (C). Expression of CD44 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. (D). Expression of ALDH1A1 in HK-2 cells, HPT cells and RPTEC/TERT1 cells. The top graph in figures A–D represent PCR data whereas the bottom graphs represent the IOD of the Western blot analysis. **** indicates significantly different in the RPTEC/TERT1 cells and HPT cells when compared to the HK-2 cells at p-value of ≤ 0.01. *** indicates significantly different in HPT cells when compared to HK-2 cells at p-value of ≤ 0.05. # indicates significantly different in the RPTEC/TERT cells when compared to the HPT cells at p-value of ≤ 0.01.

    Article Snippet: Total RNA was purified from triplicate cultures of HK-2 and RPTEC/TERT1 cells and six individual cultures of human proximal tubule (HPT) cells (single sample per culture) using the RNeasy Mini kit (Qiagen, Valencia, CA).

    Techniques: Expressing, Polymerase Chain Reaction, Western Blot

    Morphology of RPTEC/TERT1 (A) and primary cells (B) over the experimental time course. Phase-contrast images were captured at the indicated time points after seeding. Domes are visible as structures that are out of focus. Micrographs were obtained with

    Journal: Molecular and Cellular Biology

    Article Title: Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation

    doi: 10.1128/MCB.01435-12

    Figure Lengend Snippet: Morphology of RPTEC/TERT1 (A) and primary cells (B) over the experimental time course. Phase-contrast images were captured at the indicated time points after seeding. Domes are visible as structures that are out of focus. Micrographs were obtained with

    Article Snippet: At 1, 7, and 16 days after seeding, RNA was harvested from RPTEC/TERT1 cells on 10-cm dishes as described above. cDNA was synthesized from 500 ng of total RNA using a Dynamo cDNA synthesis kit (Biozyme). qPCRs were performed using 5× HOT FIREPol EvaGreen qPCR Mix Plus (Medibena) on a Rotor-Gene Q (Qiagen) according to the manufacturer's protocol.

    Techniques:

    (A) Activation levels of TFs TP53, FOXO1, c-MYC, and HIF1A in RPTEC/TERT1 nuclear extracts at days 1 and 16 after seeding. Values are means plus SD ( n = 3). Statistical significance was analyzed by applying a two-tailed unpaired t test. *, P

    Journal: Molecular and Cellular Biology

    Article Title: Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation

    doi: 10.1128/MCB.01435-12

    Figure Lengend Snippet: (A) Activation levels of TFs TP53, FOXO1, c-MYC, and HIF1A in RPTEC/TERT1 nuclear extracts at days 1 and 16 after seeding. Values are means plus SD ( n = 3). Statistical significance was analyzed by applying a two-tailed unpaired t test. *, P

    Article Snippet: At 1, 7, and 16 days after seeding, RNA was harvested from RPTEC/TERT1 cells on 10-cm dishes as described above. cDNA was synthesized from 500 ng of total RNA using a Dynamo cDNA synthesis kit (Biozyme). qPCRs were performed using 5× HOT FIREPol EvaGreen qPCR Mix Plus (Medibena) on a Rotor-Gene Q (Qiagen) according to the manufacturer's protocol.

    Techniques: Activation Assay, Two Tailed Test

    Functional grouping of genes exhibiting time-dependent expression during epithelial monolayer maturation. Genes were ranked within the allocated group according to the difference between day 1 and 16 values in the RPTEC/TERT1 data set. Cell colors reflect

    Journal: Molecular and Cellular Biology

    Article Title: Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation

    doi: 10.1128/MCB.01435-12

    Figure Lengend Snippet: Functional grouping of genes exhibiting time-dependent expression during epithelial monolayer maturation. Genes were ranked within the allocated group according to the difference between day 1 and 16 values in the RPTEC/TERT1 data set. Cell colors reflect

    Article Snippet: At 1, 7, and 16 days after seeding, RNA was harvested from RPTEC/TERT1 cells on 10-cm dishes as described above. cDNA was synthesized from 500 ng of total RNA using a Dynamo cDNA synthesis kit (Biozyme). qPCRs were performed using 5× HOT FIREPol EvaGreen qPCR Mix Plus (Medibena) on a Rotor-Gene Q (Qiagen) according to the manufacturer's protocol.

    Techniques: Functional Assay, Expressing

    Downregulation of HSPB7 in RCC. qPCR analysis shows that HSPB7 mRNA expression was significantly downregulated (A) in 11 (85%) of 13 RCC tissues compared with the normal renal tissue, and (B) in all the five RCC cell lines compared with normal HEK 293 and RPTEC cells. T and N represent RCC tissue sample and normal renal tissue, respectively. B2M (β2 microglobulin) was used for normalization of expression levels. Values are expressed as the mean ± SD. (C) IHC analysis of a tissue array consisting of 11 pairs of human RCC sample reveals that the expression of HSPB7 was significantly higher in normal kidney tissues than in RCC tissues. According to the intensity of HSPB7 staining, these samples were evaluated as: negative (−), weakly positive (+), moderate positive (++), and strong positive (+++). HSPB7 negative or weakly positive (−/+) were considered low expression, and moderate or strong positive were considered high expression (++/+++). Summary of the IHC results is shown in Table I .

    Journal: International Journal of Oncology

    Article Title: Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation

    doi: 10.3892/ijo.2014.2314

    Figure Lengend Snippet: Downregulation of HSPB7 in RCC. qPCR analysis shows that HSPB7 mRNA expression was significantly downregulated (A) in 11 (85%) of 13 RCC tissues compared with the normal renal tissue, and (B) in all the five RCC cell lines compared with normal HEK 293 and RPTEC cells. T and N represent RCC tissue sample and normal renal tissue, respectively. B2M (β2 microglobulin) was used for normalization of expression levels. Values are expressed as the mean ± SD. (C) IHC analysis of a tissue array consisting of 11 pairs of human RCC sample reveals that the expression of HSPB7 was significantly higher in normal kidney tissues than in RCC tissues. According to the intensity of HSPB7 staining, these samples were evaluated as: negative (−), weakly positive (+), moderate positive (++), and strong positive (+++). HSPB7 negative or weakly positive (−/+) were considered low expression, and moderate or strong positive were considered high expression (++/+++). Summary of the IHC results is shown in Table I .

    Article Snippet: Bisulfite sequencing Genomic DNA was extracted from RPTEC, HEK293 and 5 RCC cell lines (Caki-1, Caki-2, 786-O, A-498, ACHN) using the DNeasy blood and tissue kit (Qiagen).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Immunohistochemistry, Staining