rpmi1640  (ATCC)


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    Name:
    RPMI 1640 Medium
    Description:
    RPMI 1640 medium modified to contain 2 mM L glutamine 10 mM HEPES 1 mM sodium pyruvate 4500 mg L glucose and 1500 mg L sodium bicarbonate for use in incubators using 5 CO2 in air Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2
    Catalog Number:
    30-2001
    Price:
    None
    Applications:
    RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
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    Structured Review

    ATCC rpmi1640
    RPMI 1640 medium modified to contain 2 mM L glutamine 10 mM HEPES 1 mM sodium pyruvate 4500 mg L glucose and 1500 mg L sodium bicarbonate for use in incubators using 5 CO2 in air Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2
    https://www.bioz.com/result/rpmi1640/product/ATCC
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Identification of uPAR-positive Chemoresistant Cells in Small Cell Lung Cancer
    Article Snippet: .. Immunostaining and Flow Cytometry Analysis Primary (lung) small cell lung carcinoma (SCLC) cell lines (H1688, H1417, H69AR), bone marrow (BM) metastatic SCLC (H211, H1882) and brain metastatic SCLC (H250) cell lines were obtained from human primary lung and metastatic tissues ( ATCC), grown in RPMI 1640 modified medium (ATCC, N: 30–2001) supplemented with 10% Fetal Bovine Serum (FBS). .. The BM metastatic cell line (H1882) was cultured in complete HITES medium (D-MEM/F-12, N: 30–2006 supplemented with insulin 5 µg/mL, transferrin 10 µg/mL, sodium selenite 30 nM, hydrocortisone 10 nM, β-estradiol 10 nM, L-glutamine 2 mM, HEPES 10 mM and 5% FBS).

    Cytometry:

    Article Title: Identification of uPAR-positive Chemoresistant Cells in Small Cell Lung Cancer
    Article Snippet: .. Immunostaining and Flow Cytometry Analysis Primary (lung) small cell lung carcinoma (SCLC) cell lines (H1688, H1417, H69AR), bone marrow (BM) metastatic SCLC (H211, H1882) and brain metastatic SCLC (H250) cell lines were obtained from human primary lung and metastatic tissues ( ATCC), grown in RPMI 1640 modified medium (ATCC, N: 30–2001) supplemented with 10% Fetal Bovine Serum (FBS). .. The BM metastatic cell line (H1882) was cultured in complete HITES medium (D-MEM/F-12, N: 30–2006 supplemented with insulin 5 µg/mL, transferrin 10 µg/mL, sodium selenite 30 nM, hydrocortisone 10 nM, β-estradiol 10 nM, L-glutamine 2 mM, HEPES 10 mM and 5% FBS).

    Cell Culture:

    Article Title: Non-Thermal Plasma Couples Oxidative Stress to TRAIL Sensitization through DR5 Upregulation
    Article Snippet: .. HCT116 and HT-29 cells were cultured in RPMI-1640 containing 10% FBS and antibiotics. .. Plasma-activated medium (PAM) was produced using a microplasma jet device at atmospheric pressure [ , , ] and discharging the plasma jet onto a liquid such as the mammalian cell culture medium DMEM ( a) [ ].

    Article Title: Rapid dissolution of ZnO nanoparticles induced by biological buffers significantly impacts cytotoxicity
    Article Snippet: .. Cells were cultured in RPMI 1640 containing 10 mM HEPES and supplemented with 10% FBS (fetal bovine serum), 1% penicillin/streptomycin, and 2 mM L-glutamine per ATCC (American Type Culture Collection, Rockville, MD) recommendation. .. Cells were maintained in log phase and seeded at a concentration of 5×105 cells/mL in a 96-well plate for viability assays.

    Article Title: Nanoencapsulation Enhances Epigallocatechin-3-Gallate Stability and Its Anti-atherogenic Bioactivities in Macrophages
    Article Snippet: .. Human monocytic THP-1 cell line was purchased from the American Type Tissue Culture Collection (ATCC, Manassas, VA) and cultured in the RPMI1640 medium following to ATCC instructions. ..

    other:

    Article Title: Cancer-Derived Transforming Growth Factor-β Modulates Tumor-Associated Macrophages in Ampullary Cancer
    Article Snippet: The THP-1 monocytes were maintained in a RPMI-1640 medium with 10% FBS.

    Immunostaining:

    Article Title: Identification of uPAR-positive Chemoresistant Cells in Small Cell Lung Cancer
    Article Snippet: .. Immunostaining and Flow Cytometry Analysis Primary (lung) small cell lung carcinoma (SCLC) cell lines (H1688, H1417, H69AR), bone marrow (BM) metastatic SCLC (H211, H1882) and brain metastatic SCLC (H250) cell lines were obtained from human primary lung and metastatic tissues ( ATCC), grown in RPMI 1640 modified medium (ATCC, N: 30–2001) supplemented with 10% Fetal Bovine Serum (FBS). .. The BM metastatic cell line (H1882) was cultured in complete HITES medium (D-MEM/F-12, N: 30–2006 supplemented with insulin 5 µg/mL, transferrin 10 µg/mL, sodium selenite 30 nM, hydrocortisone 10 nM, β-estradiol 10 nM, L-glutamine 2 mM, HEPES 10 mM and 5% FBS).

    Modification:

    Article Title: Identification of uPAR-positive Chemoresistant Cells in Small Cell Lung Cancer
    Article Snippet: .. Immunostaining and Flow Cytometry Analysis Primary (lung) small cell lung carcinoma (SCLC) cell lines (H1688, H1417, H69AR), bone marrow (BM) metastatic SCLC (H211, H1882) and brain metastatic SCLC (H250) cell lines were obtained from human primary lung and metastatic tissues ( ATCC), grown in RPMI 1640 modified medium (ATCC, N: 30–2001) supplemented with 10% Fetal Bovine Serum (FBS). .. The BM metastatic cell line (H1882) was cultured in complete HITES medium (D-MEM/F-12, N: 30–2006 supplemented with insulin 5 µg/mL, transferrin 10 µg/mL, sodium selenite 30 nM, hydrocortisone 10 nM, β-estradiol 10 nM, L-glutamine 2 mM, HEPES 10 mM and 5% FBS).

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    ATCC wnt3a expressing cell
    <t>WNT3A,</t> R-Spondin 3, Noggin, EGF, IGF-1, SB202190, and DEX Treatments Promoted Intestinal Epithelial Cell Differentiation (A) The procedure for small intestinal epithelial-like cell differentiation from human iPSCs (Tic) is shown. Intestinal progenitor cells were treated with solvent (DMSO), 10 nM LY2090314, 10 μM SB202190, 10 mM nicotinamide, 1 μM DEX, 1 μM triiodothyronine (T3), 10 μM SB431542, 20 ng/mL HGF, 50 ng/mL EGF, 20 ng/mL IGF-1, 25% WNT3A CM (conditioned medium of WNT3A-expressing cells), 25% WRN CM (conditioned medium of WNT3A, R-spondin 3, Noggin-expressing cells), 10 nM [Leu15]-gastrin 1, and 100 nM trichostatin A for 12 days, or with 5 μM BIO +10 μM DAPT for 16 days. (B and C) The gene expression levels of villin 1 and ISX were examined by real-time RT-PCR analysis. On the y axis, the gene expression levels in the DMSO-treated cells were taken as 1.0. All data are presented as means ± SEM of triplicate experiments. Significant differences between the vehicle and drug-treated groups were evaluated by one-way ANOVA followed by Dunnett's post hoc test ( ∗ p
    Wnt3a Expressing Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt3a expressing cell/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    wnt3a expressing cell - by Bioz Stars, 2020-09
    99/100 stars
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    99
    ATCC rpmi1640 medium
    Stability of 100 μM of nonencapsulated EGCG, NLCE and CSNLCE in <t>RPMI1640</t> medium at 37°C in the absence of SOD (A) ; or in the presence of 5U/mL of SOD (B) . Means at a time point without a common superscript differ, P
    Rpmi1640 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640 medium/product/ATCC
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 medium - by Bioz Stars, 2020-09
    99/100 stars
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    88
    ATCC rpmi1640 biochrome
    Stability of 100 μM of nonencapsulated EGCG, NLCE and CSNLCE in <t>RPMI1640</t> medium at 37°C in the absence of SOD (A) ; or in the presence of 5U/mL of SOD (B) . Means at a time point without a common superscript differ, P
    Rpmi1640 Biochrome, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640 biochrome/product/ATCC
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 biochrome - by Bioz Stars, 2020-09
    88/100 stars
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    Image Search Results


    WNT3A, R-Spondin 3, Noggin, EGF, IGF-1, SB202190, and DEX Treatments Promoted Intestinal Epithelial Cell Differentiation (A) The procedure for small intestinal epithelial-like cell differentiation from human iPSCs (Tic) is shown. Intestinal progenitor cells were treated with solvent (DMSO), 10 nM LY2090314, 10 μM SB202190, 10 mM nicotinamide, 1 μM DEX, 1 μM triiodothyronine (T3), 10 μM SB431542, 20 ng/mL HGF, 50 ng/mL EGF, 20 ng/mL IGF-1, 25% WNT3A CM (conditioned medium of WNT3A-expressing cells), 25% WRN CM (conditioned medium of WNT3A, R-spondin 3, Noggin-expressing cells), 10 nM [Leu15]-gastrin 1, and 100 nM trichostatin A for 12 days, or with 5 μM BIO +10 μM DAPT for 16 days. (B and C) The gene expression levels of villin 1 and ISX were examined by real-time RT-PCR analysis. On the y axis, the gene expression levels in the DMSO-treated cells were taken as 1.0. All data are presented as means ± SEM of triplicate experiments. Significant differences between the vehicle and drug-treated groups were evaluated by one-way ANOVA followed by Dunnett's post hoc test ( ∗ p

    Journal: Stem Cell Reports

    Article Title: Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies

    doi: 10.1016/j.stemcr.2018.10.019

    Figure Lengend Snippet: WNT3A, R-Spondin 3, Noggin, EGF, IGF-1, SB202190, and DEX Treatments Promoted Intestinal Epithelial Cell Differentiation (A) The procedure for small intestinal epithelial-like cell differentiation from human iPSCs (Tic) is shown. Intestinal progenitor cells were treated with solvent (DMSO), 10 nM LY2090314, 10 μM SB202190, 10 mM nicotinamide, 1 μM DEX, 1 μM triiodothyronine (T3), 10 μM SB431542, 20 ng/mL HGF, 50 ng/mL EGF, 20 ng/mL IGF-1, 25% WNT3A CM (conditioned medium of WNT3A-expressing cells), 25% WRN CM (conditioned medium of WNT3A, R-spondin 3, Noggin-expressing cells), 10 nM [Leu15]-gastrin 1, and 100 nM trichostatin A for 12 days, or with 5 μM BIO +10 μM DAPT for 16 days. (B and C) The gene expression levels of villin 1 and ISX were examined by real-time RT-PCR analysis. On the y axis, the gene expression levels in the DMSO-treated cells were taken as 1.0. All data are presented as means ± SEM of triplicate experiments. Significant differences between the vehicle and drug-treated groups were evaluated by one-way ANOVA followed by Dunnett's post hoc test ( ∗ p

    Article Snippet: In brief, for the definitive endoderm cell differentiation, human iPSCs were cultured for 4 days in WNT3A-expressing cell (ATCC; CRL2647)-conditioned RPMI1640 medium (Sigma-Aldrich) containing 100 ng/mL activin A (R & D Systems), 1× GlutaMAX (Thermo Fisher Scientific), 0.2% fetal bovine serum (FBS), and 1× B27 Supplement Minus Vitamin A (Thermo Fisher Scientific).

    Techniques: Cell Differentiation, Expressing, Quantitative RT-PCR

    WNT3A, R-Spondin 3, Noggin, EGF, IGF-1, SB202190, and DEX Treatments Promoted Intestinal Epithelial Cell Differentiation (A) The procedure for small intestinal epithelial-like cell differentiation from human iPSCs (Tic) is shown. Intestinal progenitor cells were treated with solvent (DMSO), 10 nM LY2090314, 10 μM SB202190, 10 mM nicotinamide, 1 μM DEX, 1 μM triiodothyronine (T3), 10 μM SB431542, 20 ng/mL HGF, 50 ng/mL EGF, 20 ng/mL IGF-1, 25% WNT3A CM (conditioned medium of WNT3A-expressing cells), 25% WRN CM (conditioned medium of WNT3A, R-spondin 3, Noggin-expressing cells), 10 nM [Leu15]-gastrin 1, and 100 nM trichostatin A for 12 days, or with 5 μM BIO +10 μM DAPT for 16 days. (B and C) The gene expression levels of villin 1 and ISX were examined by real-time RT-PCR analysis. On the y axis, the gene expression levels in the DMSO-treated cells were taken as 1.0. All data are presented as means ± SEM of triplicate experiments. Significant differences between the vehicle and drug-treated groups were evaluated by one-way ANOVA followed by Dunnett's post hoc test ( ∗ p

    Journal: Stem Cell Reports

    Article Title: Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies

    doi: 10.1016/j.stemcr.2018.10.019

    Figure Lengend Snippet: WNT3A, R-Spondin 3, Noggin, EGF, IGF-1, SB202190, and DEX Treatments Promoted Intestinal Epithelial Cell Differentiation (A) The procedure for small intestinal epithelial-like cell differentiation from human iPSCs (Tic) is shown. Intestinal progenitor cells were treated with solvent (DMSO), 10 nM LY2090314, 10 μM SB202190, 10 mM nicotinamide, 1 μM DEX, 1 μM triiodothyronine (T3), 10 μM SB431542, 20 ng/mL HGF, 50 ng/mL EGF, 20 ng/mL IGF-1, 25% WNT3A CM (conditioned medium of WNT3A-expressing cells), 25% WRN CM (conditioned medium of WNT3A, R-spondin 3, Noggin-expressing cells), 10 nM [Leu15]-gastrin 1, and 100 nM trichostatin A for 12 days, or with 5 μM BIO +10 μM DAPT for 16 days. (B and C) The gene expression levels of villin 1 and ISX were examined by real-time RT-PCR analysis. On the y axis, the gene expression levels in the DMSO-treated cells were taken as 1.0. All data are presented as means ± SEM of triplicate experiments. Significant differences between the vehicle and drug-treated groups were evaluated by one-way ANOVA followed by Dunnett's post hoc test ( ∗ p

    Article Snippet: In brief, for the definitive endoderm cell differentiation, human iPSCs were cultured for 4 days in WNT3A-expressing cell (ATCC; CRL2647)-conditioned RPMI1640 medium (Sigma-Aldrich) containing 100 ng/mL activin A (R & D Systems), 1× GlutaMAX (Thermo Fisher Scientific), 0.2% fetal bovine serum (FBS), and 1× B27 Supplement Minus Vitamin A (Thermo Fisher Scientific).

    Techniques: Cell Differentiation, Expressing, Quantitative RT-PCR

    Stability of 100 μM of nonencapsulated EGCG, NLCE and CSNLCE in RPMI1640 medium at 37°C in the absence of SOD (A) ; or in the presence of 5U/mL of SOD (B) . Means at a time point without a common superscript differ, P

    Journal: Journal of agricultural and food chemistry

    Article Title: Nanoencapsulation Enhances Epigallocatechin-3-Gallate Stability and Its Anti-atherogenic Bioactivities in Macrophages

    doi: 10.1021/jf4023004

    Figure Lengend Snippet: Stability of 100 μM of nonencapsulated EGCG, NLCE and CSNLCE in RPMI1640 medium at 37°C in the absence of SOD (A) ; or in the presence of 5U/mL of SOD (B) . Means at a time point without a common superscript differ, P

    Article Snippet: Human monocytic THP-1 cell line was purchased from the American Type Tissue Culture Collection (ATCC, Manassas, VA) and cultured in the RPMI1640 medium following to ATCC instructions.

    Techniques:

    Stability of 100 μM of nonencapsulated EGCG, NLCE and CSNLCE in RPMI1640 medium in the presence of THP-1 derived macrophages at 4°C in the absence of SOD (A) ; at 4°C in the presence of 5U/mL of SOD (B); at 37°C in the absence of SOD (C) ; or at 37°C in the presence of 5U/mL of SOD (D) . Means at a time point without a common superscript differ, P

    Journal: Journal of agricultural and food chemistry

    Article Title: Nanoencapsulation Enhances Epigallocatechin-3-Gallate Stability and Its Anti-atherogenic Bioactivities in Macrophages

    doi: 10.1021/jf4023004

    Figure Lengend Snippet: Stability of 100 μM of nonencapsulated EGCG, NLCE and CSNLCE in RPMI1640 medium in the presence of THP-1 derived macrophages at 4°C in the absence of SOD (A) ; at 4°C in the presence of 5U/mL of SOD (B); at 37°C in the absence of SOD (C) ; or at 37°C in the presence of 5U/mL of SOD (D) . Means at a time point without a common superscript differ, P

    Article Snippet: Human monocytic THP-1 cell line was purchased from the American Type Tissue Culture Collection (ATCC, Manassas, VA) and cultured in the RPMI1640 medium following to ATCC instructions.

    Techniques: Derivative Assay

    Macrophage EGCG content. THP-1 derived macrophages were treated with 100 μM of nonencapsulated EGCG, NLCE, and CSNLCE in RPMI1640 medium containing SOD (5U/mL) for 2 and 4 hours at 4°C or 37°C. Three independent experiments were conducted. Compared to nonencapsulated EGCG, CSNLCE increased EGCG content *, P

    Journal: Journal of agricultural and food chemistry

    Article Title: Nanoencapsulation Enhances Epigallocatechin-3-Gallate Stability and Its Anti-atherogenic Bioactivities in Macrophages

    doi: 10.1021/jf4023004

    Figure Lengend Snippet: Macrophage EGCG content. THP-1 derived macrophages were treated with 100 μM of nonencapsulated EGCG, NLCE, and CSNLCE in RPMI1640 medium containing SOD (5U/mL) for 2 and 4 hours at 4°C or 37°C. Three independent experiments were conducted. Compared to nonencapsulated EGCG, CSNLCE increased EGCG content *, P

    Article Snippet: Human monocytic THP-1 cell line was purchased from the American Type Tissue Culture Collection (ATCC, Manassas, VA) and cultured in the RPMI1640 medium following to ATCC instructions.

    Techniques: Derivative Assay