rpmi1640 basic  (Thermo Fisher)


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    Name:
    RPMI 1640 Medium
    Description:
    RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer Roswell Park Memorial Institute RPMI 1640 Medium has since been found suitable for a variety of mammalian cells including HeLa Jurkat MCF 7 PC12 PBMC astrocytes and carcinomas We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications Find the right formulation using the media selector tool This RPMI is modified as follows WithWithout• L glutamine• HEPES• Phenol RedThe complete formulation is available Using RPMIRPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins RPMI 1640 Medium contains biotin vitamin B12 and PABA which are not found in Eagle s Minimal Essential Medium or Dulbecco s Modified Eagle Medium In addition the vitamins inositol and choline are present in very high concentrations RPMI 1640 Medium contains no proteins lipids or growth factors Therefore RPMI 1640 Medium requires supplementation commonly with 10 Fetal Bovine Serum FBS RPMI 1640 Medium uses a sodium bicarbonate buffer system 2 0 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH Product useFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law Customers using Gibco RPMI 1640 in a manufacturing process who have a submission with the FDA may request a letter of authorization from us to reference our Type II Drug Master File DMF cGMP manufacturing and quality systemRPMI 1640 Medium is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical RPMI 1640 product made in our Scotland facility 21875 059 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
    Catalog Number:
    11875085
    Price:
    None
    Applications:
    Cell Culture|Mammalian Cell Culture
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher rpmi1640 basic
    RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer Roswell Park Memorial Institute RPMI 1640 Medium has since been found suitable for a variety of mammalian cells including HeLa Jurkat MCF 7 PC12 PBMC astrocytes and carcinomas We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications Find the right formulation using the media selector tool This RPMI is modified as follows WithWithout• L glutamine• HEPES• Phenol RedThe complete formulation is available Using RPMIRPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins RPMI 1640 Medium contains biotin vitamin B12 and PABA which are not found in Eagle s Minimal Essential Medium or Dulbecco s Modified Eagle Medium In addition the vitamins inositol and choline are present in very high concentrations RPMI 1640 Medium contains no proteins lipids or growth factors Therefore RPMI 1640 Medium requires supplementation commonly with 10 Fetal Bovine Serum FBS RPMI 1640 Medium uses a sodium bicarbonate buffer system 2 0 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH Product useFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law Customers using Gibco RPMI 1640 in a manufacturing process who have a submission with the FDA may request a letter of authorization from us to reference our Type II Drug Master File DMF cGMP manufacturing and quality systemRPMI 1640 Medium is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity we offer an identical RPMI 1640 product made in our Scotland facility 21875 059 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
    https://www.bioz.com/result/rpmi1640 basic/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 basic - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages
    Article Snippet: .. The isolated macrophages were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) in an incubator with 5% CO2 . .. HeLa cells were transiently transfected with pFPN-EGFP using FuGENE HD transfection reagent (Promega, #E2311).

    Cell Culture:

    Article Title: The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages
    Article Snippet: .. The isolated macrophages were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) in an incubator with 5% CO2 . .. HeLa cells were transiently transfected with pFPN-EGFP using FuGENE HD transfection reagent (Promega, #E2311).

    Article Title: Hydrogen Peroxide Produced by Oral Streptococci Induces Macrophage Cell Death
    Article Snippet: .. Cell culture The human monocyte cell line THP-1 cells were purchased from RIKEN Bioresource Center and cultured in RPMI1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum (FBS) (Invitrogen) (5% FBS RPMI1640), penicillin (100 U/ml), and streptomycin (100 µg/ml) at 37°C in a 5% CO2 atmosphere. .. Differentiated THP-1 macrophages were prepared by treating THP-1 cells with 100 nM phorbol myristate acetate (PMA) (Sigma Aldrich, St. Louis, MO, USA) for 2 days.

    Infection:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: .. At 1 d p.i., cells were treated with complete RPMI 1640 medium with 2 mM 3-MA, or complete RPMI 1640 medium with 2 mM 3-MA plus 5× MEM essential amino acids solution (Invitrogen, 11130-051), and E. chaffeensis infection was assessed at 3 d p.i. .. THP-1 cells at 1.5 × 105 were preincubated with 0.1 μM rapamycin (Sigma, R0395) or 0.1% DMSO (control) for 2.5 h, and E. chaffeensis was added at an MOI of 50:1 in the continued presence of rapamycin or DMSO at 2 d p.i.

    Competitive Binding Assay:

    Article Title: Effect of sulphation on the oestrogen agonist activity of the phytoestrogens genistein and daidzein in MCF-7 human breast cancer cells
    Article Snippet: .. Competitive binding assay to ER of MCF-7 cytosol MCF-7 human breast cancer cells were grown as monolayer cultures in phenol red-free RPMI 1640 medium (Invitrogen) with 5% dextran-charcoal-stripped FCS (DCFCS; ) for a minimum of 3 days to deplete steroid hormone levels in the cells. ..

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  • 99
    Thermo Fisher rpmi1640 basic medium
    Rpmi1640 Basic Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640 basic medium/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 basic medium - by Bioz Stars, 2020-09
    99/100 stars
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