rpe  (Qiagen)


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    Structured Review

    Qiagen rpe
    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) <t>RW1</t> × 2 and <t>RPE</t> (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.
    Rpe, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpe/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpe - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues"

    Article Title: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

    Journal: BMC Research Notes

    doi: 10.1186/1756-0500-5-45

    Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) RW1 × 2 and RPE (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.
    Figure Legend Snippet: Development of RNA extraction protocol . A : RNA extracted from Arabidopsis leaves. Lane 1, RNA extraction using Logemann et al.'s protocol [ 4 ]. Lanes 2 and 3, RNA obtained by transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit). Lanes 4 and 5, RNA obtained from transfer through an RNA collection column (RNeasy). EtOH, 96% ethanol; M, DNA 1-kb ladder. B : RNA yield following extraction in 8 M guanidine hydrochloride buffer and 0.5 volume of 96% EtOH and transfer through a plasmid DNA extraction column (QIAprep Spin Miniprep Kit), followed by 2-3 washes in: (1) RW1 × 2 and RPE (RNeasy Plant Mini Kit), (2) 3 M Na-acetate and 70-75% EtOH; (3) two washes with 96% EtOH; (4) 3 M Na-acetate and PB (QIAprep); (5) two washes with PB. RNA yield was measured spectrophotometrically.

    Techniques Used: RNA Extraction, Plasmid Preparation, DNA Extraction

    Related Articles

    Plasmid Preparation:

    Article Title: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues
    Article Snippet: .. Once we established that plasmid DNA spin columns can bind RNA with the use of commercial solutions (RNeasy Plant Mini Kit), we compared the efficiencies of five solution combinations for the washing step: (1) RW1 and RPE (RNeasy Plant Mini Kit buffers, Qiagen), (2) 3 M Na-acetate and 75% EtOH, (3) 96% EtOH, (4) 3 M Na-acetate and PB buffer (QIAprep binding buffer, Qiagen), and (5) PB only (Figure ). ..

    Binding Assay:

    Article Title: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues
    Article Snippet: .. Once we established that plasmid DNA spin columns can bind RNA with the use of commercial solutions (RNeasy Plant Mini Kit), we compared the efficiencies of five solution combinations for the washing step: (1) RW1 and RPE (RNeasy Plant Mini Kit buffers, Qiagen), (2) 3 M Na-acetate and 75% EtOH, (3) 96% EtOH, (4) 3 M Na-acetate and PB buffer (QIAprep binding buffer, Qiagen), and (5) PB only (Figure ). ..

    Spectrophotometry:

    Article Title: A Fairy Chemical Suppresses Retinal Angiogenesis as a HIF Inhibitor
    Article Snippet: Quantitative PCR Total RNA from 661W cells was dissolved in TRI reagent (MRC Global, Cincinnati, OH, USA) and transferred to Econospin columns for RNA extraction and collection (GeneDesign, Osaka, Japan). .. The columns were washed with buffer RWT and RPE (Qiagen, Hilden, Germany), and the RNA samples were analyzed by ND-2000 spectrophotometer (Thermo Fisher Scientific, DE, USA) to determine the quantity and quality of the samples. .. RT-PCR was performed using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO, Osaka, Japan).

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    Qiagen rpe cells
    A: Representative images of immunofluorescence for cre (red) and L-ferritin (green) on <t>RPE</t> cells of 9-month-old mice of different genotypes. Only cre-positive cells of Bcre + , Cp −/− , <t>Heph</t> F/F mice have increased L-ferritin levels. B: Quantification
    Rpe Cells, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpe cells/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpe cells - by Bioz Stars, 2021-04
    86/100 stars
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    Image Search Results


    A: Representative images of immunofluorescence for cre (red) and L-ferritin (green) on RPE cells of 9-month-old mice of different genotypes. Only cre-positive cells of Bcre + , Cp −/− , Heph F/F mice have increased L-ferritin levels. B: Quantification

    Journal: The American Journal of Pathology

    Article Title: Ferroxidase Hephaestin's Cell-Autonomous Role in the Retinal Pigment Epithelium

    doi: 10.1016/j.ajpath.2011.12.041

    Figure Lengend Snippet: A: Representative images of immunofluorescence for cre (red) and L-ferritin (green) on RPE cells of 9-month-old mice of different genotypes. Only cre-positive cells of Bcre + , Cp −/− , Heph F/F mice have increased L-ferritin levels. B: Quantification

    Article Snippet: Bcre + , Cp −/− , Heph F/F RPE cells do not accumulate as much iron as Cp −/− , Heph sla/sla RPE cells; RPE cells in Cp −/− , Heph sla/sla mice still have residual Heph ferroxidase function, but cre-positive RPE cells in Bcre + , Cp −/− , Heph F/F mice should not.

    Techniques: Immunofluorescence, Mouse Assay

    A: Area of RPE cell atrophy ( arrow ). B: A large vacuole, extruded RPE cell, and area of RPE atrophy ( arrow ). C: A hypertrophic RPE cell. D: Extruded RPE cells in a 6-month-old Bcre + , Cp −/− , Heph F/F mouse. E: Extruded RPE cells ( arrows

    Journal: The American Journal of Pathology

    Article Title: Ferroxidase Hephaestin's Cell-Autonomous Role in the Retinal Pigment Epithelium

    doi: 10.1016/j.ajpath.2011.12.041

    Figure Lengend Snippet: A: Area of RPE cell atrophy ( arrow ). B: A large vacuole, extruded RPE cell, and area of RPE atrophy ( arrow ). C: A hypertrophic RPE cell. D: Extruded RPE cells in a 6-month-old Bcre + , Cp −/− , Heph F/F mouse. E: Extruded RPE cells ( arrows

    Article Snippet: Bcre + , Cp −/− , Heph F/F RPE cells do not accumulate as much iron as Cp −/− , Heph sla/sla RPE cells; RPE cells in Cp −/− , Heph sla/sla mice still have residual Heph ferroxidase function, but cre-positive RPE cells in Bcre + , Cp −/− , Heph F/F mice should not.

    Techniques: