rpa1 a300 241a antibodies  (Bethyl)

 
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  • 99
    Name:
    Rabbit anti RPA70 Antibody Affinity Purified
    Description:

    Catalog Number:
    A300-241A
    Price:
    [339.0]
    Applications:
    Western Blot,Immunoprecipitation,Immunofluorescence,IHC (Paraffin),Proximity Ligation Assay
    Host:
    Rabbit
    Conjugate:
    Unconjugated
    Size:
    100 ug
    Category:
    Antibody
    Antibody Type:
    Primary antibody
    Isotype:
    IgG
    Reactivity:
    Human Mouse
    Buy from Supplier


    Structured Review

    Bethyl rpa1 a300 241a antibodies

    https://www.bioz.com/result/rpa1 a300 241a antibodies/product/Bethyl
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpa1 a300 241a antibodies - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair **
    Article Snippet: .. The following antibodies were used at the indicated dilutions: CHK1 (sc8408, Santa Cruz Biotechnology, 1/1000), CHK1-pS345 (2348, Cell Signaling Technology, 1/10,000), CHK2-pT68 (2661, Cell Signaling Technology, 1/500), DNA-PKcs (MS-369-P1, Thermo Scientific, 1/200), GFP (11814460001, Roche, 1/5000), γH2AX (05-636, Millipore, 1/1000), H3 (ab1791, Abcam, 1/50,000), H3-pS10 (ab14955, Abcam, 1/5000), Ku70 (ab3114, Abcam, 1/1000), Ku80 (MS-285-P1, Thermo Scientific, 1/2000), p21 (sc397, Santa Cruz Biotechnology, 1/1000), p53 (554293, BD Biosciences, 1/6000), PAXX (ab126353, Abcam, 1/500), RPA1, (A300-241A, Bethyl Laboratories, 1/1000), TopBP1 (A300-111A, Bethyl Laboratories, 1/5000), XLF (ab33499, Abcam, 1/650) and XRCC4 (ab145, Abcam, 1/3000). .. For analyses of γH2AX foci, cells grown on glass coverslips were treated with 2 Gy of IR delivered by a CellRad system (Faxitron).

    Construct:

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Plasmid constructs—RHINO cDNA was obtained from the Human ORFeome clone set and PCR sub-cloned into pFastBac1 and pcDNA3 for expression in insect and mammalian cells. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    Incubation:

    Article Title: Protection of telomeres 1 proteins POT1a and POT1b can repress ATR signaling by RPA exclusion, but binding to CST limits ATR repression by POT1b
    Article Snippet: .. After immunoblotting, the membranes were blocked in TBS with Tween 20 with 2.5% nonfat dry milk and incubated with the following primary antibodies α-POT1a (no. 1221), α-Myc (9B11, Cell Signaling Technology), α-TIN2 (no. 1447), α-Rap1 (no. 1253), α-RPA32 (A300–244A, Bethyl), α-RPA70 (A300–241A, Bethyl), α-POT1b (no. 1223), α-STN1 (sc-376450, Santa Cruz Biotechnology), α-tubulin (GTU88, Abcam) in 1% nonfat dry milk. .. Immunoblots for POT1a were performed using the renaturation protocol described previously ( ).

    Expressing:

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Custom anti-RHINO antisera was generated by injecting rabbits with 6xHis-tagged RHINO produced in E. coli (Cocalico Biologicals Inc.) and was used to detect RHINO expression by immunoblotting. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    Western Blot:

    Article Title: The helicase domain of Polθ counteracts RPA to promote alt-NHEJ
    Article Snippet: Paragraph title: Western blot analysis ... The following primary antibodies were utilized: Myc (9E10; Calbiochem); γ-tubulin (clone GTU-88, Sigma); Flag (anti-Flag M2, Sigma); Rad51 (H2 sc8349, Santa Cruz); RPA1 (A300-241A, Bethyl).

    Article Title: Protection of telomeres 1 proteins POT1a and POT1b can repress ATR signaling by RPA exclusion, but binding to CST limits ATR repression by POT1b
    Article Snippet: After immunoblotting, the membranes were blocked in TBS with Tween 20 with 2.5% nonfat dry milk and incubated with the following primary antibodies α-POT1a (no. 1221), α-Myc (9B11, Cell Signaling Technology), α-TIN2 (no. 1447), α-Rap1 (no. 1253), α-RPA32 (A300–244A, Bethyl), α-RPA70 (A300–241A, Bethyl), α-POT1b (no. 1223), α-STN1 (sc-376450, Santa Cruz Biotechnology), α-tubulin (GTU88, Abcam) in 1% nonfat dry milk. .. Immunoblots for POT1a were performed using the renaturation protocol described previously ( ).

    Article Title: RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells
    Article Snippet: Paragraph title: Western blot analysis ... Blots were then detected for the following primary antibodies: PCNA (Santa Cruz Biotechnology, sc-56), phospho-PLK1 (Cell Signaling Technology, 5472S), PLK1 (Abcam, ab17056), ATR (Santa Cruz Biotechnology, sc-1887), phospho-S345 Chk1 (Cell Signaling Technology, 2348s), MCM3 (Bethyl Laboratories, A300-124A), phosphor-H2AX (Millipore, 05-636), RFC1 (Abcam, ab3566), RPA70 (Bethyl Laboratories, A300-241A), Sumo2/3 (Millipore, 07-2167), and ubiquitin (Santa Cruz Biotechnology, sc-8017).

    Article Title: Mechanism of Release and Fate of Excised Oligonucleotides during Nucleotide Excision Repair *
    Article Snippet: RPA and XPA were detected with antibodies from Bethyl (A300–241A) and Santa Cruz (sc-853), respectively. .. Chemiluminescent detection of proteins was done with either Western blotting luminol reagent (sc-2048; Santa Cruz) or ECL Prime reagent (GE Healthcare).

    Article Title: PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair **
    Article Snippet: Paragraph title: Antibodies, SDS-PAGE and western blotting ... The following antibodies were used at the indicated dilutions: CHK1 (sc8408, Santa Cruz Biotechnology, 1/1000), CHK1-pS345 (2348, Cell Signaling Technology, 1/10,000), CHK2-pT68 (2661, Cell Signaling Technology, 1/500), DNA-PKcs (MS-369-P1, Thermo Scientific, 1/200), GFP (11814460001, Roche, 1/5000), γH2AX (05-636, Millipore, 1/1000), H3 (ab1791, Abcam, 1/50,000), H3-pS10 (ab14955, Abcam, 1/5000), Ku70 (ab3114, Abcam, 1/1000), Ku80 (MS-285-P1, Thermo Scientific, 1/2000), p21 (sc397, Santa Cruz Biotechnology, 1/1000), p53 (554293, BD Biosciences, 1/6000), PAXX (ab126353, Abcam, 1/500), RPA1, (A300-241A, Bethyl Laboratories, 1/1000), TopBP1 (A300-111A, Bethyl Laboratories, 1/5000), XLF (ab33499, Abcam, 1/650) and XRCC4 (ab145, Abcam, 1/3000).

    Recombinase Polymerase Amplification:

    Article Title: Nucleotide Excision Repair in Human Cells: FATE OF THE EXCISED OLIGONUCLEOTIDE CARRYING DNA DAMAGE IN VIVO*
    Article Snippet: .. RPA and XPA were detected with antibodies from Bethyl (catalog no. A300-241A) and Santa Cruz Biotechnology (catalog no. sc-853), respectively. .. XPG was detected with antibodies from Santa Cruz Biotechnology (catalog no. sc-13563) for in vitro reactions and Bethyl (catalog no. A301-484A) for in vivo IP reactions.

    Article Title: Mechanism of Release and Fate of Excised Oligonucleotides during Nucleotide Excision Repair *
    Article Snippet: .. RPA and XPA were detected with antibodies from Bethyl (A300–241A) and Santa Cruz (sc-853), respectively. .. Chemiluminescent detection of proteins was done with either Western blotting luminol reagent (sc-2048; Santa Cruz) or ECL Prime reagent (GE Healthcare).

    Cell Culture:

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Chemicals and antibodies—Hydroxyurea (HU; 10 mM) and cytosine β-D-arabinofuranoside (AraC; 100 μM) were purchased from Sigma and were added to cell culture medium, where indicated, 30 min prior to UV irradiation. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    Generated:

    Article Title: ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex
    Article Snippet: Anti-phospho KAP1 (S824) antibody was generated by Cocalico biologicals, anti-phospho ATR (T1989) and anti-phospho RPA32 (S4/8) antibodies were generated by GenScript. .. The following antibodies were used: anti-phospho RPA32 (S4/8) (Bethyl Laboratories: A300-245A, 1:2000), anti-phospho RPA32 (T21) (Abcam: ab109394, 1:2000), anti-phospho RPA32 (S33) (Bethyl Laboratories: A300-246A-M, 1:2000), anti-RPA70 (Bethyl Laboratories: A300-241A, 1:2000, 1: 5000), anti-RPA32 (Bethyl Laboratories: A300-244A, 1:5000, Santa Cruz Biotechnology: sc-56770, 1:2000), anti-γH2AX (EMD Millipore Crop: 2884537, 1:1000, Bethyl Laboratories: A300-081A, 1:1000), anti-PCNA (CST: 2586, 1: 2000), anti-H3 (Proteintech, 17168-1-AP, 1:5000), anti-phospho Chk1 (S345) (CST: 2348, 1:1000), anti-Chk1 (Santa Cruz Biotechnology: sc-8408, 1:2000), anti-phospho Chk2 (T68) (CST: 2197S, 1:1000), anti-Chk2 (CST: 6334, 1:2000), anti-phospho ATR (T1989) (Genetex: GTX128145, 1:1000), anti-ATR (CST: 13934, 1: 1000, Abcam: ab2905, 1:1000, Novus: nb100-308, 1:1000), anti-TopBP1 (Bethyl Laboratories: A300-111A, 1:1000), anti-Rad17 (Santa Cruz: sc-17761, 1:1000), anti-ATRIP (CST: 2737, 1:1000), anti-GAPDH (Proteintech: 60004-1-lg, 1:10,000), anti-β-actin (Sigma: A2228, 1:2000), anti-HA (Santa Cruz: sc805, 1:2000, Sigma: H6908, 1:2000), anti-Flag (Sigma: F1804, 1:2000, F7425, 1: 2000), anti-Myc (Santa Cruz: sc-40, 1: 1000, sc764, 1:1000), anti-GFP (Santa Cruz: sc-9996, 1:1000), anti-CD71 (eBioscience: 11-0711-82, 1:100), anti SMC1 (Epitomics: 2437-1, 1:1000), anti-phospho SMC1 (S957) (CST: 4805, 1:1000), anti-phospho SMC1 (S966) (Bethyl Laboratories: A300-050A, 1:1000), and anti-KAP1 (Bethyl Laboratories: A300-274A, 1:1000).

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Custom anti-RHINO antisera was generated by injecting rabbits with 6xHis-tagged RHINO produced in E. coli (Cocalico Biologicals Inc.) and was used to detect RHINO expression by immunoblotting. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    other:

    Article Title: In Vitro Analysis of the Role of Replication Protein A (RPA) and RPA Phosphorylation in ATR-mediated Checkpoint Signaling *
    Article Snippet: p53 phospho-Ser15 antibodies (9284) and Rad17 phospho-Ser645 antibodies (3421) were purchased from Cell Signaling Technology, RPA2 phospho-Ser33 (A300–246A) and RPA1 (A300–241A) antibodies were from Bethyl Laboratories, GST (sc-138) antibodies were purchased from Santa Cruz Biotechnology, anti-FLAG M2 antibodies (F3165) were from Sigma, anti-RPA2 (NA-18) was purchased from Calbiochem, and mouse monoclonal antibodies against Rad17 were a kind gift from Lan Bo Chen ( ).

    Article Title: HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation
    Article Snippet: The commercially available antibodies used in the present study were rabbit polyclonal antibodies against HERC2 (epitope 4784–4834, Bethyl Laboratories, A301-905A), RPA1 (Bethyl Laboratories, A300-241A), BLM (Bethyl Laboratories, A300-110A), WRN (Bethyl Laboratories, A300-239A), ATR (Bethyl Laboratories, A300-138A), RFWD3 (abcam, ab99306), Phospho-S345 Chk1 (Cell Signaling, 2341), Phospho-S317 Chk1 (R & D Systems, AF2054), RPA2 (Bethyl Laboratorries, A300-244A), Phospho-S33 RPA2 (Bethyl Laboratories, A300-246A), Phospho-S4/8 RPA2 (Bethyl Laboratories, A300-245A), Phospho-T21 RPA2 (abcam, ab61065), and mouse monoclonal antibodies against HERC2 (epitope 1781–1974, BD Biosciences, 17), StrepII (Sigma-Aldrich, SAB2702215), Chk1 (Santa Cruz Biotechnology, G4) α-tubulin (Neomarkers, DM1A), Myc (BabCo, 9E10), FLAG (Sigma-Aldrich, M2), HA (Boehringer, 12CA5), RPA2 (Calbiochem, RPA34-19), conjugated ubiquitin (NIPPON BIO-TEST Laboratories, FK2), and G4 (Absolute antibody, BG4).

    Polymerase Chain Reaction:

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Plasmid constructs—RHINO cDNA was obtained from the Human ORFeome clone set and PCR sub-cloned into pFastBac1 and pcDNA3 for expression in insect and mammalian cells. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    Recombinant:

    Article Title: Regulation of DNA Repair through De-SUMOylation and SUMOylation of Replication Protein A Complex
    Article Snippet: Paragraph title: Plasmids, siRNAs, recombinant proteins, and antibodies ... Rabbit anti-RPA70 anti-Rad51AP1, and anti γ-H2AX polyclonal antibodies were from Bethyl Lab (Montgomery, TX).

    Immunofluorescence:

    Article Title: Regulation of DNA Repair through De-SUMOylation and SUMOylation of Replication Protein A Complex
    Article Snippet: The coding region of SENP6, EKPKYEPNPHYHEN, was used as animmunogen to generate rabbit anti-SENP6 polyclonal antibodies, which was further purified for immunoblot and immunofluorescence staining. .. Rabbit anti-RPA70 anti-Rad51AP1, and anti γ-H2AX polyclonal antibodies were from Bethyl Lab (Montgomery, TX).

    In Vivo:

    Article Title: Nucleotide Excision Repair in Human Cells: FATE OF THE EXCISED OLIGONUCLEOTIDE CARRYING DNA DAMAGE IN VIVO*
    Article Snippet: RPA and XPA were detected with antibodies from Bethyl (catalog no. A300-241A) and Santa Cruz Biotechnology (catalog no. sc-853), respectively. .. XPG was detected with antibodies from Santa Cruz Biotechnology (catalog no. sc-13563) for in vitro reactions and Bethyl (catalog no. A301-484A) for in vivo IP reactions.

    Mutagenesis:

    Article Title: ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex
    Article Snippet: All ZFP161 truncated mutants were generated by site-directed mutagenesis. .. The following antibodies were used: anti-phospho RPA32 (S4/8) (Bethyl Laboratories: A300-245A, 1:2000), anti-phospho RPA32 (T21) (Abcam: ab109394, 1:2000), anti-phospho RPA32 (S33) (Bethyl Laboratories: A300-246A-M, 1:2000), anti-RPA70 (Bethyl Laboratories: A300-241A, 1:2000, 1: 5000), anti-RPA32 (Bethyl Laboratories: A300-244A, 1:5000, Santa Cruz Biotechnology: sc-56770, 1:2000), anti-γH2AX (EMD Millipore Crop: 2884537, 1:1000, Bethyl Laboratories: A300-081A, 1:1000), anti-PCNA (CST: 2586, 1: 2000), anti-H3 (Proteintech, 17168-1-AP, 1:5000), anti-phospho Chk1 (S345) (CST: 2348, 1:1000), anti-Chk1 (Santa Cruz Biotechnology: sc-8408, 1:2000), anti-phospho Chk2 (T68) (CST: 2197S, 1:1000), anti-Chk2 (CST: 6334, 1:2000), anti-phospho ATR (T1989) (Genetex: GTX128145, 1:1000), anti-ATR (CST: 13934, 1: 1000, Abcam: ab2905, 1:1000, Novus: nb100-308, 1:1000), anti-TopBP1 (Bethyl Laboratories: A300-111A, 1:1000), anti-Rad17 (Santa Cruz: sc-17761, 1:1000), anti-ATRIP (CST: 2737, 1:1000), anti-GAPDH (Proteintech: 60004-1-lg, 1:10,000), anti-β-actin (Sigma: A2228, 1:2000), anti-HA (Santa Cruz: sc805, 1:2000, Sigma: H6908, 1:2000), anti-Flag (Sigma: F1804, 1:2000, F7425, 1: 2000), anti-Myc (Santa Cruz: sc-40, 1: 1000, sc764, 1:1000), anti-GFP (Santa Cruz: sc-9996, 1:1000), anti-CD71 (eBioscience: 11-0711-82, 1:100), anti SMC1 (Epitomics: 2437-1, 1:1000), anti-phospho SMC1 (S957) (CST: 4805, 1:1000), anti-phospho SMC1 (S966) (Bethyl Laboratories: A300-050A, 1:1000), and anti-KAP1 (Bethyl Laboratories: A300-274A, 1:1000).

    Bicinchoninic Acid Protein Assay:

    Article Title: RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells
    Article Snippet: Blots were then detected for the following primary antibodies: PCNA (Santa Cruz Biotechnology, sc-56), phospho-PLK1 (Cell Signaling Technology, 5472S), PLK1 (Abcam, ab17056), ATR (Santa Cruz Biotechnology, sc-1887), phospho-S345 Chk1 (Cell Signaling Technology, 2348s), MCM3 (Bethyl Laboratories, A300-124A), phosphor-H2AX (Millipore, 05-636), RFC1 (Abcam, ab3566), RPA70 (Bethyl Laboratories, A300-241A), Sumo2/3 (Millipore, 07-2167), and ubiquitin (Santa Cruz Biotechnology, sc-8017). .. Blots were then detected for the following primary antibodies: PCNA (Santa Cruz Biotechnology, sc-56), phospho-PLK1 (Cell Signaling Technology, 5472S), PLK1 (Abcam, ab17056), ATR (Santa Cruz Biotechnology, sc-1887), phospho-S345 Chk1 (Cell Signaling Technology, 2348s), MCM3 (Bethyl Laboratories, A300-124A), phosphor-H2AX (Millipore, 05-636), RFC1 (Abcam, ab3566), RPA70 (Bethyl Laboratories, A300-241A), Sumo2/3 (Millipore, 07-2167), and ubiquitin (Santa Cruz Biotechnology, sc-8017).

    Purification:

    Article Title: Regulation of DNA Repair through De-SUMOylation and SUMOylation of Replication Protein A Complex
    Article Snippet: The coding region of SENP6, EKPKYEPNPHYHEN, was used as animmunogen to generate rabbit anti-SENP6 polyclonal antibodies, which was further purified for immunoblot and immunofluorescence staining. .. Rabbit anti-RPA70 anti-Rad51AP1, and anti γ-H2AX polyclonal antibodies were from Bethyl Lab (Montgomery, TX).

    Staining:

    Article Title: Regulation of DNA Repair through De-SUMOylation and SUMOylation of Replication Protein A Complex
    Article Snippet: The coding region of SENP6, EKPKYEPNPHYHEN, was used as animmunogen to generate rabbit anti-SENP6 polyclonal antibodies, which was further purified for immunoblot and immunofluorescence staining. .. Rabbit anti-RPA70 anti-Rad51AP1, and anti γ-H2AX polyclonal antibodies were from Bethyl Lab (Montgomery, TX).

    SDS Page:

    Article Title: The helicase domain of Polθ counteracts RPA to promote alt-NHEJ
    Article Snippet: Lysates from 105 cells were loaded on an SDS/PAGE and transferred to a nitrocellulose membrane. .. The following primary antibodies were utilized: Myc (9E10; Calbiochem); γ-tubulin (clone GTU-88, Sigma); Flag (anti-Flag M2, Sigma); Rad51 (H2 sc8349, Santa Cruz); RPA1 (A300-241A, Bethyl).

    Article Title: Protection of telomeres 1 proteins POT1a and POT1b can repress ATR signaling by RPA exclusion, but binding to CST limits ATR repression by POT1b
    Article Snippet: Harvested MEFs (1 × 106 cells) were resuspended in 100 μl 2× Laemmli buffer, incubated with 250 units Benzonase (Sigma-Aldrich) on ice for 10 min, denatured at 95 °C for 5 min and separated on 8–16% SDS-PAGE (2 × 105 cell equivalent per lane). .. After immunoblotting, the membranes were blocked in TBS with Tween 20 with 2.5% nonfat dry milk and incubated with the following primary antibodies α-POT1a (no. 1221), α-Myc (9B11, Cell Signaling Technology), α-TIN2 (no. 1447), α-Rap1 (no. 1253), α-RPA32 (A300–244A, Bethyl), α-RPA70 (A300–241A, Bethyl), α-POT1b (no. 1223), α-STN1 (sc-376450, Santa Cruz Biotechnology), α-tubulin (GTU88, Abcam) in 1% nonfat dry milk.

    Article Title: RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells
    Article Snippet: The total protein quantities were normalized, separated by SDS-PAGE gel, and transferred to 0.45 μM polyvinylidene fluoride (PVDF) membranes. .. Blots were then detected for the following primary antibodies: PCNA (Santa Cruz Biotechnology, sc-56), phospho-PLK1 (Cell Signaling Technology, 5472S), PLK1 (Abcam, ab17056), ATR (Santa Cruz Biotechnology, sc-1887), phospho-S345 Chk1 (Cell Signaling Technology, 2348s), MCM3 (Bethyl Laboratories, A300-124A), phosphor-H2AX (Millipore, 05-636), RFC1 (Abcam, ab3566), RPA70 (Bethyl Laboratories, A300-241A), Sumo2/3 (Millipore, 07-2167), and ubiquitin (Santa Cruz Biotechnology, sc-8017).

    Article Title: Mechanism of Release and Fate of Excised Oligonucleotides during Nucleotide Excision Repair *
    Article Snippet: Proteins in CFE, excision reactions, and immunoprecipitates were separated by SDS-PAGE and transferred to Hybond ECL membranes (GE Healthcare). .. RPA and XPA were detected with antibodies from Bethyl (A300–241A) and Santa Cruz (sc-853), respectively.

    Article Title: PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair **
    Article Snippet: Paragraph title: Antibodies, SDS-PAGE and western blotting ... The following antibodies were used at the indicated dilutions: CHK1 (sc8408, Santa Cruz Biotechnology, 1/1000), CHK1-pS345 (2348, Cell Signaling Technology, 1/10,000), CHK2-pT68 (2661, Cell Signaling Technology, 1/500), DNA-PKcs (MS-369-P1, Thermo Scientific, 1/200), GFP (11814460001, Roche, 1/5000), γH2AX (05-636, Millipore, 1/1000), H3 (ab1791, Abcam, 1/50,000), H3-pS10 (ab14955, Abcam, 1/5000), Ku70 (ab3114, Abcam, 1/1000), Ku80 (MS-285-P1, Thermo Scientific, 1/2000), p21 (sc397, Santa Cruz Biotechnology, 1/1000), p53 (554293, BD Biosciences, 1/6000), PAXX (ab126353, Abcam, 1/500), RPA1, (A300-241A, Bethyl Laboratories, 1/1000), TopBP1 (A300-111A, Bethyl Laboratories, 1/5000), XLF (ab33499, Abcam, 1/650) and XRCC4 (ab145, Abcam, 1/3000).

    Plasmid Preparation:

    Article Title: ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex
    Article Snippet: Plasmids and antibodies pCMV6–Myc-ZFP161 vector was purchased from ORIGENE. .. The following antibodies were used: anti-phospho RPA32 (S4/8) (Bethyl Laboratories: A300-245A, 1:2000), anti-phospho RPA32 (T21) (Abcam: ab109394, 1:2000), anti-phospho RPA32 (S33) (Bethyl Laboratories: A300-246A-M, 1:2000), anti-RPA70 (Bethyl Laboratories: A300-241A, 1:2000, 1: 5000), anti-RPA32 (Bethyl Laboratories: A300-244A, 1:5000, Santa Cruz Biotechnology: sc-56770, 1:2000), anti-γH2AX (EMD Millipore Crop: 2884537, 1:1000, Bethyl Laboratories: A300-081A, 1:1000), anti-PCNA (CST: 2586, 1: 2000), anti-H3 (Proteintech, 17168-1-AP, 1:5000), anti-phospho Chk1 (S345) (CST: 2348, 1:1000), anti-Chk1 (Santa Cruz Biotechnology: sc-8408, 1:2000), anti-phospho Chk2 (T68) (CST: 2197S, 1:1000), anti-Chk2 (CST: 6334, 1:2000), anti-phospho ATR (T1989) (Genetex: GTX128145, 1:1000), anti-ATR (CST: 13934, 1: 1000, Abcam: ab2905, 1:1000, Novus: nb100-308, 1:1000), anti-TopBP1 (Bethyl Laboratories: A300-111A, 1:1000), anti-Rad17 (Santa Cruz: sc-17761, 1:1000), anti-ATRIP (CST: 2737, 1:1000), anti-GAPDH (Proteintech: 60004-1-lg, 1:10,000), anti-β-actin (Sigma: A2228, 1:2000), anti-HA (Santa Cruz: sc805, 1:2000, Sigma: H6908, 1:2000), anti-Flag (Sigma: F1804, 1:2000, F7425, 1: 2000), anti-Myc (Santa Cruz: sc-40, 1: 1000, sc764, 1:1000), anti-GFP (Santa Cruz: sc-9996, 1:1000), anti-CD71 (eBioscience: 11-0711-82, 1:100), anti SMC1 (Epitomics: 2437-1, 1:1000), anti-phospho SMC1 (S957) (CST: 4805, 1:1000), anti-phospho SMC1 (S966) (Bethyl Laboratories: A300-050A, 1:1000), and anti-KAP1 (Bethyl Laboratories: A300-274A, 1:1000).

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Plasmid constructs—RHINO cDNA was obtained from the Human ORFeome clone set and PCR sub-cloned into pFastBac1 and pcDNA3 for expression in insect and mammalian cells. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    Irradiation:

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Chemicals and antibodies—Hydroxyurea (HU; 10 mM) and cytosine β-D-arabinofuranoside (AraC; 100 μM) were purchased from Sigma and were added to cell culture medium, where indicated, 30 min prior to UV irradiation. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    In Vitro:

    Article Title: Nucleotide Excision Repair in Human Cells: FATE OF THE EXCISED OLIGONUCLEOTIDE CARRYING DNA DAMAGE IN VIVO*
    Article Snippet: RPA and XPA were detected with antibodies from Bethyl (catalog no. A300-241A) and Santa Cruz Biotechnology (catalog no. sc-853), respectively. .. XPG was detected with antibodies from Santa Cruz Biotechnology (catalog no. sc-13563) for in vitro reactions and Bethyl (catalog no. A301-484A) for in vivo IP reactions.

    Column Chromatography:

    Article Title: Regulation of DNA Repair through De-SUMOylation and SUMOylation of Replication Protein A Complex
    Article Snippet: Recombinant Rad51were purified by a combination of metal-chelate affinity and Mono Q ion exchange column chromatography. .. Rabbit anti-RPA70 anti-Rad51AP1, and anti γ-H2AX polyclonal antibodies were from Bethyl Lab (Montgomery, TX).

    Produced:

    Article Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling
    Article Snippet: Custom anti-RHINO antisera was generated by injecting rabbits with 6xHis-tagged RHINO produced in E. coli (Cocalico Biologicals Inc.) and was used to detect RHINO expression by immunoblotting. .. Additional antibodies used for immunoblotting included anti-MEK2 (catalog no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalog no. A300-241A) from Bethyl Laboratories; anti-TopBP1 (catalog no. AB3245) from Millipore; anti-FLAG (catalog no. F-3165) from Sigma; anti-His antibody (catalog no. AM1010a) from Abgent; anti-Rad9 (catalog no. sc-8324), anti-Rad1 (catalog no. sc-22783), and anti-Chk1 (catalog no. sc-8408) from Santa Cruz Biotechnology; and anti-phospho-Chk1 (Ser345) (catalog no. 2348) from Cell Signaling Technology.

    Immunoprecipitation:

    Article Title: Nucleotide Excision Repair in Human Cells: FATE OF THE EXCISED OLIGONUCLEOTIDE CARRYING DNA DAMAGE IN VIVO*
    Article Snippet: Antibodies used for immunoprecipitation (IP) included anti-mouse IgG (catalog no. sc-2025), anti-rabbit IgG (catalog no. sc-2027), anti-XPB (catalog no. sc-293), anti-XPA (catalog no. sc-28353), anti-p62 (catalog no. sc-292), and anti-XPC (catalog no.sc-74410) from Santa Cruz Biotechnology; anti-RPA34 (catalog no. NA18) from Calbiochem; anti-XPG (catalog no. A301-485A) from Bethyl; anti-XPF (catalog no. ab17798) and rabbit anti-mouse IgG (catalog no. ab46540) from Abcam; anti-CPD from Kamiya Biomedical; and anti-(6-4)PP from Cosmo Bio. .. RPA and XPA were detected with antibodies from Bethyl (catalog no. A300-241A) and Santa Cruz Biotechnology (catalog no. sc-853), respectively.

    Article Title: DNA Repair Synthesis and Ligation Affect the Processing of Excised Oligonucleotides Generated by Human Nucleotide Excision Repair *
    Article Snippet: Antibodies used for immunoprecipitation included anti-p62 (Santa Cruz Biotechnology catalogue no. sc-292) and anti-RPA34 (Calbiochem catalogue no. NA18). .. Antibodies used for immunoblotting included anti-MEK2 (catalogue no. 610235) from BD Transduction Laboratories; anti-RPA70 (catalogue no. A300–241A) and anti-phospho-RPA32 (Ser-33) (catalogue no. A300–246A) from Bethyl Laboratories; anti-XPB (catalogue no. sc-293) from Santa Cruz Biotechnology; and anti-phospho-H2AX (Ser-139) (catalogue no. 2577), anti-phospho-p53 (Ser-15) (catalogue no. 9284), anti-cleaved PARP (catalogue no. 5625), anti-calnexin (catalogue no. 2679), and anti-cleaved caspase-3 (catalogue no. 9664) from Cell Signaling Technology.

    Cell Counting:

    Article Title: RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells
    Article Snippet: Blots were then detected for the following primary antibodies: PCNA (Santa Cruz Biotechnology, sc-56), phospho-PLK1 (Cell Signaling Technology, 5472S), PLK1 (Abcam, ab17056), ATR (Santa Cruz Biotechnology, sc-1887), phospho-S345 Chk1 (Cell Signaling Technology, 2348s), MCM3 (Bethyl Laboratories, A300-124A), phosphor-H2AX (Millipore, 05-636), RFC1 (Abcam, ab3566), RPA70 (Bethyl Laboratories, A300-241A), Sumo2/3 (Millipore, 07-2167), and ubiquitin (Santa Cruz Biotechnology, sc-8017). .. Blots were then detected for the following primary antibodies: PCNA (Santa Cruz Biotechnology, sc-56), phospho-PLK1 (Cell Signaling Technology, 5472S), PLK1 (Abcam, ab17056), ATR (Santa Cruz Biotechnology, sc-1887), phospho-S345 Chk1 (Cell Signaling Technology, 2348s), MCM3 (Bethyl Laboratories, A300-124A), phosphor-H2AX (Millipore, 05-636), RFC1 (Abcam, ab3566), RPA70 (Bethyl Laboratories, A300-241A), Sumo2/3 (Millipore, 07-2167), and ubiquitin (Santa Cruz Biotechnology, sc-8017).

    Lysis:

    Article Title: PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair **
    Article Snippet: Whole cell extracts were obtained by washing cells in phosphate-buffered saline (PBS) before lysis in SDS loading buffer (2% SDS, 10% (v/v) glycerol, 25 mM TCEP and 62.5 mM Tris-HCl, pH 6.8). .. The following antibodies were used at the indicated dilutions: CHK1 (sc8408, Santa Cruz Biotechnology, 1/1000), CHK1-pS345 (2348, Cell Signaling Technology, 1/10,000), CHK2-pT68 (2661, Cell Signaling Technology, 1/500), DNA-PKcs (MS-369-P1, Thermo Scientific, 1/200), GFP (11814460001, Roche, 1/5000), γH2AX (05-636, Millipore, 1/1000), H3 (ab1791, Abcam, 1/50,000), H3-pS10 (ab14955, Abcam, 1/5000), Ku70 (ab3114, Abcam, 1/1000), Ku80 (MS-285-P1, Thermo Scientific, 1/2000), p21 (sc397, Santa Cruz Biotechnology, 1/1000), p53 (554293, BD Biosciences, 1/6000), PAXX (ab126353, Abcam, 1/500), RPA1, (A300-241A, Bethyl Laboratories, 1/1000), TopBP1 (A300-111A, Bethyl Laboratories, 1/5000), XLF (ab33499, Abcam, 1/650) and XRCC4 (ab145, Abcam, 1/3000).

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    Bethyl rabbit anti rpa70 antibody affinity purified
    Rabbit Anti Rpa70 Antibody Affinity Purified, supplied by Bethyl, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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