round bottom 96 microwell  (Thermo Fisher)


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    Name:
    96 Well Plate Individual Round Well
    Description:
    Thermo Scientific Abgene polypropylene microplates and deepwell plates offer storage security for assays compound libraries or storing samples for either intermediate or long term use Abgene microtiter plates are manufactured to exacting specifications in our Class 100 000 clean room ISO 9001 conditions using high quality medical grade virgin polypropylene resins which ensure confidence in the quality and performance of the storage plates Designed to ANSI standards these microplates and deepwell plates are compatible with a variety of automated liquid handling for high throughput workflows The polypropylene storage plates are offered with spindle pyramidal U and V bottom wells along with a wide range of volumes to accommodate most applications
    Catalog Number:
    ab0564
    Price:
    None
    Applications:
    Laboratory Plastics and Supplies|Microplates, Dishes and Flasks
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    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher round bottom 96 microwell
    Thermo Scientific Abgene polypropylene microplates and deepwell plates offer storage security for assays compound libraries or storing samples for either intermediate or long term use Abgene microtiter plates are manufactured to exacting specifications in our Class 100 000 clean room ISO 9001 conditions using high quality medical grade virgin polypropylene resins which ensure confidence in the quality and performance of the storage plates Designed to ANSI standards these microplates and deepwell plates are compatible with a variety of automated liquid handling for high throughput workflows The polypropylene storage plates are offered with spindle pyramidal U and V bottom wells along with a wide range of volumes to accommodate most applications
    https://www.bioz.com/result/round bottom 96 microwell/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    round bottom 96 microwell - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting
    Article Snippet: Purified iNKT cells were seeded in round-bottom 96-wells at 1:1 ratio with irradiated (3500 rad) autologous mononuclear cells (iAPC) and activated with Dynabeads Human T-Activator CD3/CD28 (Gibco™) at 1:1 beads-to-cell ratio in T cell medium at a density of 1-5 x 104 cells per ml. .. Within 24-48 hr, activated iNKT cells were transduced with concentrated CAR lentivirus using an MOI of 2-5 in the presence of 4 μg/ml polybrene, with spinoculation for 90 min at 1000G.

    Irradiation:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: After 1 week, T-cells positive for HLA-A2/pp65495−503 tetramers were sorted and incubated with irradiated, T-cell depleted (using positive CD3+ sort from Miltenyi Biotec), PBMCs (1 × 105 cells/mL, irradiated with 70 Gy) from three healthy donors. .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression.

    Article Title: Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting
    Article Snippet: .. Purified iNKT cells were seeded in round-bottom 96-wells at 1:1 ratio with irradiated (3500 rad) autologous mononuclear cells (iAPC) and activated with Dynabeads Human T-Activator CD3/CD28 (Gibco™) at 1:1 beads-to-cell ratio in T cell medium at a density of 1-5 x 104 cells per ml. ..

    Article Title: Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor. Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor
    Article Snippet: Then, PBMC were seeded at 2 × 105 cells/well into round‐bottom 96‐microwell plates with 100 μL RPMI1640 containing 2 mmol/L l ‐glutamine (Invitrogen, Grand Island, NY, USA), 55 μmol/L 2‐mercaptoethanol (Invitrogen), 100 IU/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), and 5% (v/v) autologous plasma in the presence of each synthetic peptide (10 μg/mL). .. To establish CTL clones, tetramer‐positive T cells were seeded in 96‐well round‐bottom microplates at 1 cell per well with irradiated allogeneic PBMC (5 × 104 ) in 100 μL RPMI1640 containing 10% autologous plasma and cognate peptide (100 ng/mL).

    Flow Cytometry:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression. .. Flow Cytometry Cells were blocked in flow cytometry buffer (PBS with 0.5% BSA and 0.02% NaN3 ) supplemented with 0.5% NMS (Fitzgerald 88R-M002).

    Recombinant:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: The cells were cultured in the presence of 1 μg/mL leucoagglutinin PHA-L (Sigma-Aldrich) and 120 U/mL of recombinant human IL-2 (Immunotools) and re-stimulated and expanded during several culturing cycles before freezing in aliquots. .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression.

    Article Title: Mesenchymal Stem Cells Induce T-Cell Tolerance and Protect the Preterm Brain after Global Hypoxia-Ischemia
    Article Snippet: CFSE-labeled splenocytes were plated at a concentration of 1.0 million cells/mL in a round bottom 96-wells plate in DMEM (Gibco®, Life Technologies, Bleiswijk, The Netherlands) culture medium containing 10% FCS and 1% Penicillin Streptomycin solution. .. CFSE-labeled splenocytes were stimulated by adding Phytohemagglutinin (PHA) (Lectin; Sigma-Aldrich, Zwijndrecht, The Netherlands) at a final concentration of 1 µg/mL and human recombinant IL-2 (Proleukin®; Aldesleukin (Chiron), Novartis, Basel, Switzerland) at a final concentration of 500 IU/mL to the culture medium.

    Article Title: Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor. Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor
    Article Snippet: Then, PBMC were seeded at 2 × 105 cells/well into round‐bottom 96‐microwell plates with 100 μL RPMI1640 containing 2 mmol/L l ‐glutamine (Invitrogen, Grand Island, NY, USA), 55 μmol/L 2‐mercaptoethanol (Invitrogen), 100 IU/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), and 5% (v/v) autologous plasma in the presence of each synthetic peptide (10 μg/mL). .. After 72 hours, an equal volume of a medium containing 100 U/mL interleukin 2 (recombinant human IL‐2, S‐6820; Shionogi Pharmaceutical Co., Osaka, Japan) was added to each well, and the medium was replaced every 2 or 3 days thereafter with a fresh medium containing IL‐2 (50 U/mL).

    Clone Assay:

    Article Title: Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor. Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor
    Article Snippet: Then, PBMC were seeded at 2 × 105 cells/well into round‐bottom 96‐microwell plates with 100 μL RPMI1640 containing 2 mmol/L l ‐glutamine (Invitrogen, Grand Island, NY, USA), 55 μmol/L 2‐mercaptoethanol (Invitrogen), 100 IU/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), and 5% (v/v) autologous plasma in the presence of each synthetic peptide (10 μg/mL). .. To establish CTL clones, tetramer‐positive T cells were seeded in 96‐well round‐bottom microplates at 1 cell per well with irradiated allogeneic PBMC (5 × 104 ) in 100 μL RPMI1640 containing 10% autologous plasma and cognate peptide (100 ng/mL).

    Isolation:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression. .. Flow Cytometry Cells were blocked in flow cytometry buffer (PBS with 0.5% BSA and 0.02% NaN3 ) supplemented with 0.5% NMS (Fitzgerald 88R-M002).

    Marker:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression. .. Flow Cytometry Cells were blocked in flow cytometry buffer (PBS with 0.5% BSA and 0.02% NaN3 ) supplemented with 0.5% NMS (Fitzgerald 88R-M002).

    Cytometry:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression. .. Flow Cytometry Cells were blocked in flow cytometry buffer (PBS with 0.5% BSA and 0.02% NaN3 ) supplemented with 0.5% NMS (Fitzgerald 88R-M002).

    Cell Culture:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: The cells were cultured in the presence of 1 μg/mL leucoagglutinin PHA-L (Sigma-Aldrich) and 120 U/mL of recombinant human IL-2 (Immunotools) and re-stimulated and expanded during several culturing cycles before freezing in aliquots. .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression.

    CTL Assay:

    Article Title: Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor. Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor
    Article Snippet: Then, PBMC were seeded at 2 × 105 cells/well into round‐bottom 96‐microwell plates with 100 μL RPMI1640 containing 2 mmol/L l ‐glutamine (Invitrogen, Grand Island, NY, USA), 55 μmol/L 2‐mercaptoethanol (Invitrogen), 100 IU/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), and 5% (v/v) autologous plasma in the presence of each synthetic peptide (10 μg/mL). .. To establish CTL clones, tetramer‐positive T cells were seeded in 96‐well round‐bottom microplates at 1 cell per well with irradiated allogeneic PBMC (5 × 104 ) in 100 μL RPMI1640 containing 10% autologous plasma and cognate peptide (100 ng/mL).

    Concentration Assay:

    Article Title: Mesenchymal Stem Cells Induce T-Cell Tolerance and Protect the Preterm Brain after Global Hypoxia-Ischemia
    Article Snippet: .. CFSE-labeled splenocytes were plated at a concentration of 1.0 million cells/mL in a round bottom 96-wells plate in DMEM (Gibco®, Life Technologies, Bleiswijk, The Netherlands) culture medium containing 10% FCS and 1% Penicillin Streptomycin solution. .. CFSE-labeled splenocytes were stimulated by adding Phytohemagglutinin (PHA) (Lectin; Sigma-Aldrich, Zwijndrecht, The Netherlands) at a final concentration of 1 µg/mL and human recombinant IL-2 (Proleukin®; Aldesleukin (Chiron), Novartis, Basel, Switzerland) at a final concentration of 500 IU/mL to the culture medium.

    Incubation:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression. .. Flow Cytometry Cells were blocked in flow cytometry buffer (PBS with 0.5% BSA and 0.02% NaN3 ) supplemented with 0.5% NMS (Fitzgerald 88R-M002).

    Activation Assay:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: Paragraph title: Preparation of NLV-Expanded T-Cells and NLV Specific T-Cell Activation Assay ... EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression.

    Proliferation Assay:

    Article Title: Mesenchymal Stem Cells Induce T-Cell Tolerance and Protect the Preterm Brain after Global Hypoxia-Ischemia
    Article Snippet: Paragraph title: Proliferation assay ... CFSE-labeled splenocytes were plated at a concentration of 1.0 million cells/mL in a round bottom 96-wells plate in DMEM (Gibco®, Life Technologies, Bleiswijk, The Netherlands) culture medium containing 10% FCS and 1% Penicillin Streptomycin solution.

    Labeling:

    Article Title: Mesenchymal Stem Cells Induce T-Cell Tolerance and Protect the Preterm Brain after Global Hypoxia-Ischemia
    Article Snippet: Splenocytes were labeled with CFSE (Sigma-Aldrich, Zwijndrecht, The Netherlands) in a final concentration of 1 µM. .. CFSE-labeled splenocytes were plated at a concentration of 1.0 million cells/mL in a round bottom 96-wells plate in DMEM (Gibco®, Life Technologies, Bleiswijk, The Netherlands) culture medium containing 10% FCS and 1% Penicillin Streptomycin solution.

    Expressing:

    Article Title: Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
    Article Snippet: .. EV isolated from 200.000 moDC were added to 50.000 T-cells in a total volume of 50 μL in a round-bottom 96-wells plate (Thermo Scientific), and incubated for 4 to 5 h before assaying by flow cytometry intracellular cytokines and cell surface marker expression. .. Flow Cytometry Cells were blocked in flow cytometry buffer (PBS with 0.5% BSA and 0.02% NaN3 ) supplemented with 0.5% NMS (Fitzgerald 88R-M002).

    Modification:

    Article Title: Mesenchymal Stem Cells Induce T-Cell Tolerance and Protect the Preterm Brain after Global Hypoxia-Ischemia
    Article Snippet: Single-cell splenocyte suspensions were obtained by dissociating freshly sampled spleen tissues in gentleMACS™ C-tubes (MiltEnyi, Leiden, the Netherlands) filled with Gibco® Iscove’s Modified Dulbecco’s Medium (IMDM) (Life Technologies, Bleiswijk, the Netherlands) using the gentleMACS™ Dissociator (MiltEnyi, Leiden, the Netherlands). .. CFSE-labeled splenocytes were plated at a concentration of 1.0 million cells/mL in a round bottom 96-wells plate in DMEM (Gibco®, Life Technologies, Bleiswijk, The Netherlands) culture medium containing 10% FCS and 1% Penicillin Streptomycin solution.

    Staining:

    Article Title: Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor. Identification of novel human leukocyte antigen‐A*11:01‐restricted cytotoxic T‐lymphocyte epitopes derived from osteosarcoma antigen papillomavirus binding factor
    Article Snippet: Then, PBMC were seeded at 2 × 105 cells/well into round‐bottom 96‐microwell plates with 100 μL RPMI1640 containing 2 mmol/L l ‐glutamine (Invitrogen, Grand Island, NY, USA), 55 μmol/L 2‐mercaptoethanol (Invitrogen), 100 IU/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), and 5% (v/v) autologous plasma in the presence of each synthetic peptide (10 μg/mL). .. Tetramer staining was carried out on days 14‐21.

    Purification:

    Article Title: Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting
    Article Snippet: .. Purified iNKT cells were seeded in round-bottom 96-wells at 1:1 ratio with irradiated (3500 rad) autologous mononuclear cells (iAPC) and activated with Dynabeads Human T-Activator CD3/CD28 (Gibco™) at 1:1 beads-to-cell ratio in T cell medium at a density of 1-5 x 104 cells per ml. ..

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  • 99
    Thermo Fisher nunc 96 well polystyrene round bottom microwell plates
    Nunc 96 Well Polystyrene Round Bottom Microwell Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc 96 well polystyrene round bottom microwell plates/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nunc 96 well polystyrene round bottom microwell plates - by Bioz Stars, 2020-04
    99/100 stars
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