rosetta plyss bl21  (Thermo Fisher)


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  • 80

    Structured Review

    Thermo Fisher rosetta plyss bl21
    Rosetta Plyss Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rosetta plyss bl21/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rosetta plyss bl21 - by Bioz Stars, 2020-02
    80/100 stars

    Related Products / Commonly Used Together

    gst-tagged mkk6

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    Related Articles

    Affinity Chromatography:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. GST-MKK6 was purified by GST-Sepharose 4b (GE) affinity chromatography.

    Mutagenesis:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: .. Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. Cells were lysed by sonication in 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM DTT, 0.1% Triton X-100, 1 mM EDTA, and 10% glycerol.

    Ligand Binding Assay:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: .. Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. Cells were lysed by sonication in 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM DTT, 0.1% Triton X-100, 1 mM EDTA, and 10% glycerol.

    Purification:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. GST-MKK6 was purified by GST-Sepharose 4b (GE) affinity chromatography.

    Activation Assay:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: .. Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. Cells were lysed by sonication in 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM DTT, 0.1% Triton X-100, 1 mM EDTA, and 10% glycerol.

    Incubation:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. Protein was dialyzed into 20 mM Hepes pH 7.6, 100 mM NaCl, and 2 mM DTT. p38γ was activated (dual phosphorylation on Thr183/Tyr185) by incubation with the mutant MKK6 at room temperature in 75 mM Hepes pH 7.5, 0.1 mM PMSF, 1 mM NaF, 0.1 mM NaVO4 , 0.1 mM β-glycerol phosphate, 10 mM MgCl2 , and 1 mM DTT.

    Sonication:

    Article Title: NMR Characterization of Information Flow and Allosteric Communities in the MAP Kinase p38γ
    Article Snippet: Activation and ligand binding A constitutively active mutant of GST-tagged MKK6 (S207D, T211D) was overexpressed in Rosetta plysS BL21(DE3) (Invitrogen) E. coli cells at 293 K in LB media. .. Cells were lysed by sonication in 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM DTT, 0.1% Triton X-100, 1 mM EDTA, and 10% glycerol.