rosetta bl21  (Millipore)


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    Structured Review

    Millipore rosetta bl21
    Rosetta Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rosetta bl21/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rosetta bl21 - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Positron Emission Tomography:

    Article Title: A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli
    Article Snippet: .. The vector pET-22b and E. coli strain BL21 (DE3) were purchased from Novagen (San Diego, CA). .. Isopropyl β -D-1-thiogalactopyranoside (IPTG) was from Sigma-Aldrich (St. Louis, MO).

    Article Title: Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae
    Article Snippet: .. The recombinant plasmid, named pET_UspF, was further purified and transformed into the E. coli BL21 (DE3) pLyS strain (Novagen). .. One recombinant clone was chosen at random among the recombinant colonies and selected for further analysis for protein expression and purification.

    Synthesized:

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Construct:

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: .. Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Purification:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: .. 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. ..

    Article Title: Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae
    Article Snippet: .. The recombinant plasmid, named pET_UspF, was further purified and transformed into the E. coli BL21 (DE3) pLyS strain (Novagen). .. One recombinant clone was chosen at random among the recombinant colonies and selected for further analysis for protein expression and purification.

    Activity Assay:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: .. 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. ..

    Expressing:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: .. 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. ..

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: .. Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Transformation Assay:

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: .. Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Article Title: Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae
    Article Snippet: .. The recombinant plasmid, named pET_UspF, was further purified and transformed into the E. coli BL21 (DE3) pLyS strain (Novagen). .. One recombinant clone was chosen at random among the recombinant colonies and selected for further analysis for protein expression and purification.

    Article Title: Design and evaluation of a recombinant multi-epitope antigen for serodiagnosis of Toxoplasma gondii infection in humans
    Article Snippet: .. Consequently, the recombinant plasmid was transformed into E. coli BL21 (DE3) plysS competent cells (Novagen, USA). .. Following the confirmation of the sequences of inserts by DNA sequencing (IDT, Singapore), the protein expression was induced by isopropyl-D thiogalactopyranoside (IPTG) with a final concentration of 1 mM.

    Recombinant:

    Article Title: Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae
    Article Snippet: .. The recombinant plasmid, named pET_UspF, was further purified and transformed into the E. coli BL21 (DE3) pLyS strain (Novagen). .. One recombinant clone was chosen at random among the recombinant colonies and selected for further analysis for protein expression and purification.

    Article Title: Design and evaluation of a recombinant multi-epitope antigen for serodiagnosis of Toxoplasma gondii infection in humans
    Article Snippet: .. Consequently, the recombinant plasmid was transformed into E. coli BL21 (DE3) plysS competent cells (Novagen, USA). .. Following the confirmation of the sequences of inserts by DNA sequencing (IDT, Singapore), the protein expression was induced by isopropyl-D thiogalactopyranoside (IPTG) with a final concentration of 1 mM.

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Plasmid Preparation:

    Article Title: A simple vector system to improve performance and utilisation of recombinant antibodies
    Article Snippet: .. E. coli BL21 (DE3) and Origami 2™ (DE3) cells and the pET26b(+) plasmid were from Novagen (San Diego, CA). ..

    Article Title: A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli
    Article Snippet: .. The vector pET-22b and E. coli strain BL21 (DE3) were purchased from Novagen (San Diego, CA). .. Isopropyl β -D-1-thiogalactopyranoside (IPTG) was from Sigma-Aldrich (St. Louis, MO).

    Article Title: Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae
    Article Snippet: .. The recombinant plasmid, named pET_UspF, was further purified and transformed into the E. coli BL21 (DE3) pLyS strain (Novagen). .. One recombinant clone was chosen at random among the recombinant colonies and selected for further analysis for protein expression and purification.

    Article Title: Design and evaluation of a recombinant multi-epitope antigen for serodiagnosis of Toxoplasma gondii infection in humans
    Article Snippet: .. Consequently, the recombinant plasmid was transformed into E. coli BL21 (DE3) plysS competent cells (Novagen, USA). .. Following the confirmation of the sequences of inserts by DNA sequencing (IDT, Singapore), the protein expression was induced by isopropyl-D thiogalactopyranoside (IPTG) with a final concentration of 1 mM.

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

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    Millipore recombinant expression bl21 rosetta 2 cells
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