rneasy protect cell minikit  (Qiagen)


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    Name:
    RNeasy Protect Cell Mini Kit
    Description:
    For stabilization and purification of total RNA from cells Kit contents Qiagen RNeasy Protect Cell Mini Kit 30 to 50L Elution Volume max 10e7 cells Sample Spin Column Format Silica Technology Manual Processing Ideal for PCR qPCR Real time RT PCR Microarray Applications For Stabilization and Purification of Total RNA from Cells Includes RNAprotect Cell Reagent 50mL and RNeasy Plus Mini Kit 50 Benefits Immediate RNA stabilization in sorted or cultured cells No need to remove medium prior to cell stabilization Storage and shipping of cells without dry ice Archiving of cells at 20ºC or 80ºC Purification of high quality RNA in minu
    Catalog Number:
    74624
    Price:
    369
    Category:
    RNeasy Protect Cell Mini Kit
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    Structured Review

    Qiagen rneasy protect cell minikit
    RNeasy Protect Cell Mini Kit
    For stabilization and purification of total RNA from cells Kit contents Qiagen RNeasy Protect Cell Mini Kit 30 to 50L Elution Volume max 10e7 cells Sample Spin Column Format Silica Technology Manual Processing Ideal for PCR qPCR Real time RT PCR Microarray Applications For Stabilization and Purification of Total RNA from Cells Includes RNAprotect Cell Reagent 50mL and RNeasy Plus Mini Kit 50 Benefits Immediate RNA stabilization in sorted or cultured cells No need to remove medium prior to cell stabilization Storage and shipping of cells without dry ice Archiving of cells at 20ºC or 80ºC Purification of high quality RNA in minu
    https://www.bioz.com/result/rneasy protect cell minikit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rneasy protect cell minikit - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    β-mercaptoethanol
    rna
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    rlt buffer
    tetrahymena cells

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    Related Articles

    Amplification:

    Article Title: Respiratory Tract Epithelial Cells Express Retinaldehyde Dehydrogenase ALDH1A and Enhance IgA Production by Stimulated B Cells in the Presence of Vitamin A
    Article Snippet: PCR Analysis for ALDH1A mRNA Cells were stored in RNA Protect Cell Reagent (Qiagen, Cat#76526) at −20°C and RNA was extracted from 1×105 cells using RNeasy Plus Mini Kits (Qiagen, Cat# 74134). .. One µl of the neat or 1∶10 serially diluted cDNA (of 40 µl total) was used for PCR amplification with Platinum PCR SuperMix (Invitrogen, Cat#11306-016).

    DNA Synthesis:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. Complementary DNA synthesis was performed using the Superscript First-strand Synthesis System (Invitrogen) following the manufacturer’s protocol.

    Synthesized:

    Article Title: Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells
    Article Snippet: 2.2 Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) Total RNA was isolated from the NS-SV-DC and A5 cells with an RNAprotect Cell Mini Kit (Qiagen, Hilden, Germany) as described previously , . .. All the PCR primers were synthesized by NGRL (Sendai, Japan).

    Construct:

    Article Title: Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila
    Article Snippet: Total RNA was extracted from WT and cyc17 Δ cells at 4, 5, 6 and 7 h post meiotic induction using the RNeasy Protect Cell Mini Kit (Qiagen), as previously described (TetraFGD: ). .. Total RNA was extracted from WT and cyc17 Δ cells at 4, 5, 6 and 7 h post meiotic induction using the RNeasy Protect Cell Mini Kit (Qiagen), as previously described (TetraFGD: ).

    Article Title: RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena
    Article Snippet: Total RNA was extracted from Tetrahymena cells using the RNeasy Protect Cell minikit (Qiagen), as described (TetraFGD, ). .. First strand-specific libraries were constructed using the Illumina TruSeq Stranded mRNA sample preparation kit (RS-122-2101).

    Real-time Polymerase Chain Reaction:

    Article Title: Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells
    Article Snippet: 2.2 Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) Total RNA was isolated from the NS-SV-DC and A5 cells with an RNAprotect Cell Mini Kit (Qiagen, Hilden, Germany) as described previously , . .. The isolated RNA was reverse transcribed to the cDNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) for the real-time PCR template, as described previously , .

    Microarray:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen). .. In addition to the three principle groups (LD, RUN, CE), L-RUN and H-RUN groups were generated by pooling RNA from the mice with the four lowest and four highest running distances, yielding a total of five groups.

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen). .. In addition to the three principle groups (LD, RUN, CE), L-RUN and H-RUN groups were generated by pooling RNA from the mice with the four lowest and four highest running distances, yielding a total of five groups.

    TLDA Assay:

    Article Title: Optimizing High Dimensional Gene Expression Studies for Immune Response Following Smallpox Vaccination Using Taqman(R) Low Density Immune Arrays
    Article Snippet: Paragraph title: 2.6 TLDA assays ... Total RNA was extracted according to the manufacturer’s instructions using the RNeasy Protect Cell Mini kit (Qiagen).

    Expressing:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: Notably, mice used for RNA-Seq studies were a separate cohort from those used in behavioral studies, avoiding the possibility that gene expression measurements are altered by a previous learning experience. .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: Notably, mice used for RNA-Seq studies were a separate cohort from those used in behavioral studies, avoiding the possibility that gene expression measurements are altered by a previous learning experience. .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Gel Purification:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. The reverse transcription PCR (RT-PCR) products were separated by agarose gel electrophoresis, followed by excision from the gel, purification and sequencing using the BigDye Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems) and the 3730xl DNA Analyzer (Applied Biosystems).

    Cell Culture:

    Article Title: New Fusion Transcripts Identified in Normal Karyotype Acute Myeloid Leukemia
    Article Snippet: All cell lines were cultured in RPMI-1640, 10% fetal calf serum, 2 mM L-glutamine, 50 units/ml penicillin and 50 µg/ml streptomycin (Invitrogen). .. Total RNA was isolated from each cell line (5×106 cells) using the RNeasy Protect Cell Mini Kit (QIAGEN).

    Generated:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen). .. In addition to the three principle groups (LD, RUN, CE), L-RUN and H-RUN groups were generated by pooling RNA from the mice with the four lowest and four highest running distances, yielding a total of five groups.

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen). .. In addition to the three principle groups (LD, RUN, CE), L-RUN and H-RUN groups were generated by pooling RNA from the mice with the four lowest and four highest running distances, yielding a total of five groups.

    Polymerase Chain Reaction:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: .. Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. Complementary DNA synthesis was performed using the Superscript First-strand Synthesis System (Invitrogen) following the manufacturer’s protocol.

    Article Title: Respiratory Tract Epithelial Cells Express Retinaldehyde Dehydrogenase ALDH1A and Enhance IgA Production by Stimulated B Cells in the Presence of Vitamin A
    Article Snippet: .. PCR Analysis for ALDH1A mRNA Cells were stored in RNA Protect Cell Reagent (Qiagen, Cat#76526) at −20°C and RNA was extracted from 1×105 cells using RNeasy Plus Mini Kits (Qiagen, Cat# 74134). .. One third of the extracted RNA was used for cDNA synthesis using Superscript III (Invitrogen, Cat#18080-044).

    Article Title: Optimizing High Dimensional Gene Expression Studies for Immune Response Following Smallpox Vaccination Using Taqman(R) Low Density Immune Arrays
    Article Snippet: Total RNA was extracted according to the manufacturer’s instructions using the RNeasy Protect Cell Mini kit (Qiagen). .. 100 μl of cDNA from each sample was mixed with an equal volume of TaqMan Universal PCR Master Mix (Applied Biosystems), centrifuged, and loaded on the port on a TLDA card (Applied Biosystems).

    Article Title: CGK062, a small chemical molecule, inhibits cancer upregulated gene 2-induced oncogenesis through NEK2 and β-catenin
    Article Snippet: Reverse transcription-polymerase chain reaction (RT-PCR) For performing RT-PCR, total RNA was extracted from the cells using an RNeasy protect cell mini kit (Qiagen, Valencia, CA, USA), in accordance with the manufacturer’s instructions. .. PCR was performed using specific primers described elsewhere ( , ). cDNAs obtained for each sample were diluted, and PCR was performed using an optimized cycle number. β-actin mRNA was used as an internal standard.

    Article Title: Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells
    Article Snippet: 2.2 Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) Total RNA was isolated from the NS-SV-DC and A5 cells with an RNAprotect Cell Mini Kit (Qiagen, Hilden, Germany) as described previously , . .. The cDNA was subjected to PCR with the following primers: β-actin (NM_001101) sense primer, 5׳-GCGAGAAGATGACCCAGA-3׳ and anti-sense primer, 5׳-CAGAGGCGTACAGGGATA-3׳; REG Iα (NM_002909) sense primer 5׳-AGGAGAGTGGCACTGATGACTT-3׳ and anti-sense primer 5׳-TAGGAGACCAGGGACCCACTG-3׳; STAT3 (NM_213662) sense primer, 5׳-CAGGATGGCCCAATGGAATC-3׳ and anti-sense primer 5׳-CCCAGGAGATTATGAAACACC-3׳.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. The reverse transcription PCR (RT-PCR) products were separated by agarose gel electrophoresis, followed by excision from the gel, purification and sequencing using the BigDye Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems) and the 3730xl DNA Analyzer (Applied Biosystems).

    Article Title: CGK062, a small chemical molecule, inhibits cancer upregulated gene 2-induced oncogenesis through NEK2 and β-catenin
    Article Snippet: .. Reverse transcription-polymerase chain reaction (RT-PCR) For performing RT-PCR, total RNA was extracted from the cells using an RNeasy protect cell mini kit (Qiagen, Valencia, CA, USA), in accordance with the manufacturer’s instructions. .. Subsequently, 3 µ g total RNA were reversed transcribed into cDNA using Superscript II reverse transcriptase (Invitrogen/Thermo Fisher Scientific).

    Article Title: Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells
    Article Snippet: .. 2.2 Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) Total RNA was isolated from the NS-SV-DC and A5 cells with an RNAprotect Cell Mini Kit (Qiagen, Hilden, Germany) as described previously , . .. The isolated RNA was reverse transcribed to the cDNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) for the real-time PCR template, as described previously , .

    Injection:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    RNA Sequencing Assay:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: Paragraph title: RNA-Sequencing (RNA-Seq) and Bio-informatics ... RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Article Title: Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila
    Article Snippet: Paragraph title: RNA-Seq analysis ... Total RNA was extracted from WT and cyc17 Δ cells at 4, 5, 6 and 7 h post meiotic induction using the RNeasy Protect Cell Mini Kit (Qiagen), as previously described (TetraFGD: ).

    Article Title: RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena
    Article Snippet: Paragraph title: RNA-seq and analysis ... Total RNA was extracted from Tetrahymena cells using the RNeasy Protect Cell minikit (Qiagen), as described (TetraFGD, ).

    Isolation:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: .. Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. Complementary DNA synthesis was performed using the Superscript First-strand Synthesis System (Invitrogen) following the manufacturer’s protocol.

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen). .. In addition to the three principle groups (LD, RUN, CE), L-RUN and H-RUN groups were generated by pooling RNA from the mice with the four lowest and four highest running distances, yielding a total of five groups.

    Article Title: New Fusion Transcripts Identified in Normal Karyotype Acute Myeloid Leukemia
    Article Snippet: .. Total RNA was isolated from each cell line (5×106 cells) using the RNeasy Protect Cell Mini Kit (QIAGEN). .. Experimental validation Strand-specific RT-PCR and Sanger sequencing were used to validate the fusion transcripts identified by sequence mapping analysis.

    Article Title: Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
    Article Snippet: .. Isolation of Total RNA Qiashredder spin columns were used for homogenization followed by total RNA extraction using the RNeasy Protect Cell Mini Kit (Qiagen, Valenica, CA) according to manufacturer's instructions. .. The concentration of total RNA was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE) and RNA integrity was verified using a Bioanalyzer 1000 (Agilent, Palo Alto, CA).

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen). .. In addition to the three principle groups (LD, RUN, CE), L-RUN and H-RUN groups were generated by pooling RNA from the mice with the four lowest and four highest running distances, yielding a total of five groups.

    Article Title: RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena
    Article Snippet: Total RNA was extracted from Tetrahymena cells using the RNeasy Protect Cell minikit (Qiagen), as described (TetraFGD, ). .. Small RNA was enriched by a mirVana™ miRNA isolation kit (Ambion).

    Article Title: Epigenetic inactivation of VGF associated with Urothelial Cell Carcinoma and its potential as a non-invasive biomarker using urine
    Article Snippet: Isolation of genomic DNA from cell lines was performed with the phenol-chloroform extraction protocol followed by ethanol precipitation as previously described [ ]. .. For RNA extraction, adherent cells were detached by addition of RNA protect Cell Reagent (Qiagen), followed by the procedure of the RNeasy Plus Mini kit (Qiagen).

    Mouse Assay:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Sequencing:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. The reverse transcription PCR (RT-PCR) products were separated by agarose gel electrophoresis, followed by excision from the gel, purification and sequencing using the BigDye Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems) and the 3730xl DNA Analyzer (Applied Biosystems).

    Article Title: Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila
    Article Snippet: Total RNA was extracted from WT and cyc17 Δ cells at 4, 5, 6 and 7 h post meiotic induction using the RNeasy Protect Cell Mini Kit (Qiagen), as previously described (TetraFGD: ). .. Total RNA was extracted from WT and cyc17 Δ cells at 4, 5, 6 and 7 h post meiotic induction using the RNeasy Protect Cell Mini Kit (Qiagen), as previously described (TetraFGD: ).

    Sample Prep:

    Article Title: RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena
    Article Snippet: Total RNA was extracted from Tetrahymena cells using the RNeasy Protect Cell minikit (Qiagen), as described (TetraFGD, ). .. First strand-specific libraries were constructed using the Illumina TruSeq Stranded mRNA sample preparation kit (RS-122-2101).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Respiratory Tract Epithelial Cells Express Retinaldehyde Dehydrogenase ALDH1A and Enhance IgA Production by Stimulated B Cells in the Presence of Vitamin A
    Article Snippet: .. PCR Analysis for ALDH1A mRNA Cells were stored in RNA Protect Cell Reagent (Qiagen, Cat76526) at −20°C and RNA was extracted from 1×105 cells using RNeasy Plus Mini Kits (Qiagen, Cat# 74134). .. One third of the extracted RNA was used for cDNA synthesis using Superscript III (Invitrogen, Cat#18080-044).

    RNA Extraction:

    Article Title: Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
    Article Snippet: .. Isolation of Total RNA Qiashredder spin columns were used for homogenization followed by total RNA extraction using the RNeasy Protect Cell Mini Kit (Qiagen, Valenica, CA) according to manufacturer's instructions. .. The concentration of total RNA was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE) and RNA integrity was verified using a Bioanalyzer 1000 (Agilent, Palo Alto, CA).

    Article Title: Epigenetic inactivation of VGF associated with Urothelial Cell Carcinoma and its potential as a non-invasive biomarker using urine
    Article Snippet: .. For RNA extraction, adherent cells were detached by addition of RNA protect Cell Reagent (Qiagen), followed by the procedure of the RNeasy Plus Mini kit (Qiagen). .. RNA extraction from bladder tissue was performed with the QIAzol Lysis Reagent (Qiagen) following manufacturer protocol.

    Agarose Gel Electrophoresis:

    Article Title: A novel KCNQ4 mutation and a private IMMP2L-DOCK4 duplication segregating with nonsyndromic hearing loss in a Brazilian family
    Article Snippet: Reverse transcription PCR Total RNA was isolated from peripheral blood with the RNeasy Protect Cell Mini kit (Qiagen, Venlo, The Netherlands) and treated with DNase I (Invitrogen). .. The reverse transcription PCR (RT-PCR) products were separated by agarose gel electrophoresis, followed by excision from the gel, purification and sequencing using the BigDye Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems) and the 3730xl DNA Analyzer (Applied Biosystems).

    Homogenization:

    Article Title: Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
    Article Snippet: .. Isolation of Total RNA Qiashredder spin columns were used for homogenization followed by total RNA extraction using the RNeasy Protect Cell Mini Kit (Qiagen, Valenica, CA) according to manufacturer's instructions. .. The concentration of total RNA was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE) and RNA integrity was verified using a Bioanalyzer 1000 (Agilent, Palo Alto, CA).

    Ethanol Precipitation:

    Article Title: Epigenetic inactivation of VGF associated with Urothelial Cell Carcinoma and its potential as a non-invasive biomarker using urine
    Article Snippet: Isolation of genomic DNA from cell lines was performed with the phenol-chloroform extraction protocol followed by ethanol precipitation as previously described [ ]. .. For RNA extraction, adherent cells were detached by addition of RNA protect Cell Reagent (Qiagen), followed by the procedure of the RNeasy Plus Mini kit (Qiagen).

    Preserving:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Spectrophotometry:

    Article Title: Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
    Article Snippet: Isolation of Total RNA Qiashredder spin columns were used for homogenization followed by total RNA extraction using the RNeasy Protect Cell Mini Kit (Qiagen, Valenica, CA) according to manufacturer's instructions. .. The concentration of total RNA was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE) and RNA integrity was verified using a Bioanalyzer 1000 (Agilent, Palo Alto, CA).

    Concentration Assay:

    Article Title: Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle
    Article Snippet: Isolation of Total RNA Qiashredder spin columns were used for homogenization followed by total RNA extraction using the RNeasy Protect Cell Mini Kit (Qiagen, Valenica, CA) according to manufacturer's instructions. .. The concentration of total RNA was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Rockland, DE) and RNA integrity was verified using a Bioanalyzer 1000 (Agilent, Palo Alto, CA).

    Article Title: Optimizing High Dimensional Gene Expression Studies for Immune Response Following Smallpox Vaccination Using Taqman(R) Low Density Immune Arrays
    Article Snippet: Rested PBMC at a concentration of 4×105 /mL (500 μl/well) were plated in duplicate on 6 well tissue culture plates (Becton, Dickinson & Co., Franklin Lakes, NJ) with UV-inactivated vaccinia virus (MOI 0.5, 1 and 5 PFU/cell) diluted in RPMI 1640 culture media containing 5% FCS (Hyclone), 100 U/mL penicillin (Sigma), 100 μg/mL streptomycin (Sigma), and 1mM sodium pyruvate (Mediatech) for 12, 18, 24, 36 and 48 hours in a CO2 incubator at 37°C. .. Total RNA was extracted according to the manufacturer’s instructions using the RNeasy Protect Cell Mini kit (Qiagen).

    Lysis:

    Article Title: Epigenetic inactivation of VGF associated with Urothelial Cell Carcinoma and its potential as a non-invasive biomarker using urine
    Article Snippet: For RNA extraction, adherent cells were detached by addition of RNA protect Cell Reagent (Qiagen), followed by the procedure of the RNeasy Plus Mini kit (Qiagen). .. RNA extraction from bladder tissue was performed with the QIAzol Lysis Reagent (Qiagen) following manufacturer protocol.

    Laser Capture Microdissection:

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

    Article Title: RNA-Sequencing Reveals Unique Transcriptional Signatures of Running and Running-Independent Environmental Enrichment in the Adult Mouse Dentate Gyrus
    Article Snippet: To promote RNA preservation during the DG microdissection procedure, mice were injected with a lethal dose of Xylazine (0.1%) and Chloral hydrate (7%), then perfused trans-cardially with PBS containing sodium fluoride (50 mM), sodium pyrophosphate (1 mM) and SigmaFAST protease inhibitors (Sigma). .. RNA was isolated from snap-frozen microdissections of the entire DG using the RNeasy mini microarray kit as per manufacturer’s instructions (Qiagen).

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  • 99
    Qiagen rneasy protect cell minikit
    Rneasy Protect Cell Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy protect cell minikit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rneasy protect cell minikit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen rna extraction kit
    Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna extraction kit/product/Qiagen
    Average 99 stars, based on 940 article reviews
    Price from $9.99 to $1999.99
    rna extraction kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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