rneasy protect bacteria minikit  (Qiagen)


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    Name:
    RNeasy Protect Bacteria Mini Kit
    Description:
    For stabilization and purification of up to 100 µg total RNA from bacteria Kit contents Qiagen RNeasy Protect Bacteria mini Kit 50 preps 15 to 100g Sample 30 to 100L Elution Volume 20 min Time Run 8 to 70g Yield Spin Column Format Silica Technology Manual Processing For Stabilization and Purification of Up to 100g Total RNA from Bacteria Ideal For PCR qPCR Real time PCR Microarray Includes RNeasy mini Kit 50 and RNAprotect Bacteria Reagent 2 x 100mL Benefits Immediate in vivo RNA stabilization and protection Reliable gene expression and gene profiling data No need for liquid nitrogen dry ice or phenol Ready to use high quality total RNA in minutes No CsCl gradients no LiCl or ethanol precip
    Catalog Number:
    74524
    Price:
    502
    Category:
    RNeasy Protect Bacteria Mini Kit
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    Structured Review

    Qiagen rneasy protect bacteria minikit
    RNeasy Protect Bacteria Mini Kit
    For stabilization and purification of up to 100 µg total RNA from bacteria Kit contents Qiagen RNeasy Protect Bacteria mini Kit 50 preps 15 to 100g Sample 30 to 100L Elution Volume 20 min Time Run 8 to 70g Yield Spin Column Format Silica Technology Manual Processing For Stabilization and Purification of Up to 100g Total RNA from Bacteria Ideal For PCR qPCR Real time PCR Microarray Includes RNeasy mini Kit 50 and RNAprotect Bacteria Reagent 2 x 100mL Benefits Immediate in vivo RNA stabilization and protection Reliable gene expression and gene profiling data No need for liquid nitrogen dry ice or phenol Ready to use high quality total RNA in minutes No CsCl gradients no LiCl or ethanol precip
    https://www.bioz.com/result/rneasy protect bacteria minikit/product/Qiagen
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rneasy protect bacteria minikit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death
    Article Snippet: .. Cells were pelleted by centrifugation and RNA was extracted according to the RNeasy Protect Bacteria Mini Kit protocol (QIAGEN) with a minor modification, namely DNase digestion was performed on-column for 30 min at 37°C. .. ADP ribosylation assays For production of cell lysates, E. coli strain DH5α was cultured to an OD600 of 0.7.

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: Bacterial suspensions were stabilized with RNA protect bacterial reagent (Qiagen) before centrifugation. .. We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions.

    Article Title: Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival
    Article Snippet: Illumina RNA sequencing Bacterial cells were cultured at 10 °C until they reached their logarithmic growth phase and were collected by centrifugation. .. Total RNA was prepared using the RNeasy Protect Bacteria Mini Kit (QIAGEN Biotech Co. Ltd., Hilden, Germany) and QIAGEN RNeasy Mini Kit (QIAGEN) according to the manufacturer’s protocols.

    Amplification:

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , . .. The transcript containing the 3′ end of nlpD and the 5′ end of penA was amplified using Q5 High-Fidelity Polymerase according to the manufacturer’s recommendations using primers P2999 & P3003.

    DNA Synthesis:

    Article Title: In vitro characterization and inhibition of the interaction between ciprofloxacin and berberine against multidrug-resistant Klebsiella pneumoniae
    Article Snippet: Reverse transcription polymerase chain reaction Total RNA was extracted from five multidrug-resistant clinical K. pneumoniae isolates (R5, R12, R15, ATCC and RATCC) using the RNeasy Protect Bacteria Mini Kit (Qiagen China, Shanghai, China) and lysozyme (Sigma, Sigma-Aldrich China, Shanghai, China) following the manufacturer's protocol. acrA , acrB , acrR , tolC , ramA and marA were detected via RT-PCR using primers to examine gene expression ( ). .. The PrimeScript RT reagent kit (Takara Bio, Kyoto, Japan) was used for complementary DNA synthesis.

    Positive Control:

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , . .. Genomic DNA isolated from 1026b cells using the PureGene Core kit A (QIAGEN, Valencia, CA) was used as a positive control reaction, while DNAse treated cDNA generated from 1026b and Bp1651 RNA and sterile water were used as negative control templates.

    Synthesized:

    Article Title: Molecular Investigations of PenA-mediated ?-lactam Resistance in Burkholderia pseudomallei
    Article Snippet: .. Quantification of penA transcript levels Overnight cultures were subcultured into LB medium, grown to an OD600 nm of 0.5 and RNA was extracted with the RNeasy Protect Bacteria Mini Kit (Qiagen, Valencia, CA, USA). cDNA was synthesized using the SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen) and quantified using SYBR GreenER qPCR SuperMix for iCycler Instruments (Invitrogen) and the Bio-Rad iQ5 iCycler. ..

    Quantitative RT-PCR:

    Article Title: Molecular Investigations of PenA-mediated ?-lactam Resistance in Burkholderia pseudomallei
    Article Snippet: .. Quantification of penA transcript levels Overnight cultures were subcultured into LB medium, grown to an OD600 nm of 0.5 and RNA was extracted with the RNeasy Protect Bacteria Mini Kit (Qiagen, Valencia, CA, USA). cDNA was synthesized using the SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen) and quantified using SYBR GreenER qPCR SuperMix for iCycler Instruments (Invitrogen) and the Bio-Rad iQ5 iCycler. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation
    Article Snippet: .. RNA extraction and quantitative real-time polymerase chain reaction (qPCR) Total RNA of A . baumannii was extracted using an RNeasy Protect Bacteria Mini kit (Qiagen, Tokyo, Japan). .. To analyze the efflux pump and biofilm-related gene expression levels in A . baumannii , total RNA was extracted from the bacteria after 16 hours of incubation.

    Article Title: Molecular Investigations of PenA-mediated ?-lactam Resistance in Burkholderia pseudomallei
    Article Snippet: .. Quantification of penA transcript levels Overnight cultures were subcultured into LB medium, grown to an OD600 nm of 0.5 and RNA was extracted with the RNeasy Protect Bacteria Mini Kit (Qiagen, Valencia, CA, USA). cDNA was synthesized using the SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen) and quantified using SYBR GreenER qPCR SuperMix for iCycler Instruments (Invitrogen) and the Bio-Rad iQ5 iCycler. ..

    Random Hexamer Labeling:

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions. .. Reverse transcription was carried out from 1 µg of total RNA for gene expression analysis, by using OMNISCRIPT (Qiagen) and random hexamer primers (Promega).

    Activity Assay:

    Article Title: Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases
    Article Snippet: The CD activity from the tnaA -disrupted strain was virtually unchanged in the presence or absence of l -cysteine. .. Strain JM39 was cultivated in LB medium or LB plus 10 mM l -cysteine, and total RNA was prepared using an RNeasy Protect Bacteria Mini kit (QIAGEN, Valencia, Calif.).

    Infection:

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions. .. To extract bacterial RNA from infected plants we used a protocol for extracting RNA from P.syringae recovered from infected leaves as described by Yu et al .

    Expressing:

    Article Title: Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases
    Article Snippet: Strain JM39 was cultivated in LB medium or LB plus 10 mM l -cysteine, and total RNA was prepared using an RNeasy Protect Bacteria Mini kit (QIAGEN, Valencia, Calif.). .. 1.7 kb), while the basal level of expression in LB medium was fairly low.

    Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qPCR) Total RNA of A . baumannii was extracted using an RNeasy Protect Bacteria Mini kit (Qiagen, Tokyo, Japan). .. To analyze the efflux pump and biofilm-related gene expression levels in A . baumannii , total RNA was extracted from the bacteria after 16 hours of incubation.

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions. .. Reverse transcription was carried out from 1 µg of total RNA for gene expression analysis, by using OMNISCRIPT (Qiagen) and random hexamer primers (Promega).

    Article Title: In vitro characterization and inhibition of the interaction between ciprofloxacin and berberine against multidrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription polymerase chain reaction Total RNA was extracted from five multidrug-resistant clinical K. pneumoniae isolates (R5, R12, R15, ATCC and RATCC) using the RNeasy Protect Bacteria Mini Kit (Qiagen China, Shanghai, China) and lysozyme (Sigma, Sigma-Aldrich China, Shanghai, China) following the manufacturer's protocol. acrA , acrB , acrR , tolC , ramA and marA were detected via RT-PCR using primers to examine gene expression ( ). ..

    Article Title: Molecular Investigations of PenA-mediated ?-lactam Resistance in Burkholderia pseudomallei
    Article Snippet: Quantification of penA transcript levels Overnight cultures were subcultured into LB medium, grown to an OD600 nm of 0.5 and RNA was extracted with the RNeasy Protect Bacteria Mini Kit (Qiagen, Valencia, CA, USA). cDNA was synthesized using the SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen) and quantified using SYBR GreenER qPCR SuperMix for iCycler Instruments (Invitrogen) and the Bio-Rad iQ5 iCycler. .. For induction studies with both wild-type and the mutants with constitutive regulatory gene expression or deletion several methods were employed.

    Modification:

    Article Title: An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death
    Article Snippet: .. Cells were pelleted by centrifugation and RNA was extracted according to the RNeasy Protect Bacteria Mini Kit protocol (QIAGEN) with a minor modification, namely DNase digestion was performed on-column for 30 min at 37°C. .. ADP ribosylation assays For production of cell lysates, E. coli strain DH5α was cultured to an OD600 of 0.7.

    Derivative Assay:

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , . .. Copy DNAs derived from transcripts internal to nlpD1 and penA were amplified with P2997 & P2998 and P3001 & P2293), respectively.

    Northern Blot:

    Article Title: Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases
    Article Snippet: Northern blot analysis for tnaA was carried out by using a Gene Images Random-Prime Labeling and Detection System (Amersham Pharmacia Biotech, Buckinghamshire, England). .. Strain JM39 was cultivated in LB medium or LB plus 10 mM l -cysteine, and total RNA was prepared using an RNeasy Protect Bacteria Mini kit (QIAGEN, Valencia, Calif.).

    Cell Culture:

    Article Title: An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death
    Article Snippet: For isolation of total RNA, E. coli DH5α cells were cultured in LB medium to an OD600 of 0.7, mixed with RNAprotect Bacteria Reagent (QIAGEN) by vortexing followed by incubation for 5 min. at room temperature. .. Cells were pelleted by centrifugation and RNA was extracted according to the RNeasy Protect Bacteria Mini Kit protocol (QIAGEN) with a minor modification, namely DNase digestion was performed on-column for 30 min at 37°C.

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: For RNA isolation from in vitro cultured bacteria, Pst DC3000 was grown on KB agar plates for 48 h at 28°C. .. We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions.

    Article Title: Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival
    Article Snippet: Illumina RNA sequencing Bacterial cells were cultured at 10 °C until they reached their logarithmic growth phase and were collected by centrifugation. .. Total RNA was prepared using the RNeasy Protect Bacteria Mini Kit (QIAGEN Biotech Co. Ltd., Hilden, Germany) and QIAGEN RNeasy Mini Kit (QIAGEN) according to the manufacturer’s protocols.

    Generated:

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , . .. Genomic DNA isolated from 1026b cells using the PureGene Core kit A (QIAGEN, Valencia, CA) was used as a positive control reaction, while DNAse treated cDNA generated from 1026b and Bp1651 RNA and sterile water were used as negative control templates.

    Article Title: Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans
    Article Snippet: Culture at Separate Growth Phases and RNA Extraction Planktonic cultures were generated by overnight incubation with shaking at 180 rpm of a single colony transferred to 10 ml of BHI media. .. The cultures were grown with shaking at 180 rpm to an OD600nm of 0.8, which corresponds to the early stationary growth phase , and then stabilized by treatment with RNA Protect (RNeasy Protect Bacteria Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s recommendations.

    other:

    Article Title: Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents
    Article Snippet: Total RNA was extracted with an RNeasy Protect Bacteria Mini Kit with on-column DNase digestion (Qiagen).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Characterization of the MSMEG_2631 Gene (mmp) Encoding a Multidrug and Toxic Compound Extrusion (MATE) Family Protein in Mycobacterium smegmatis and Exploration of Its Polyspecific Nature Using Biolog Phenotype MicroArray
    Article Snippet: Paragraph title: RNA extraction and RT-PCR. ... 0.8 to 0.9) using the RNeasy Protect Bacteria Mini Kit (Qiagen).

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: Paragraph title: RT-PCR ... Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , .

    Article Title: In vitro characterization and inhibition of the interaction between ciprofloxacin and berberine against multidrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription polymerase chain reaction Total RNA was extracted from five multidrug-resistant clinical K. pneumoniae isolates (R5, R12, R15, ATCC and RATCC) using the RNeasy Protect Bacteria Mini Kit (Qiagen China, Shanghai, China) and lysozyme (Sigma, Sigma-Aldrich China, Shanghai, China) following the manufacturer's protocol. acrA , acrB , acrR , tolC , ramA and marA were detected via RT-PCR using primers to examine gene expression ( ). ..

    Sonication:

    Article Title: Listeria monocytogenes ?B Regulates Stress Response and Virulence Functions
    Article Snippet: RNA was extracted from L. monocytogenes either grown to stationary phase or exposed to osmotic stress (as described below) with the RNeasy Protect Bacteria midi kit (Qiagen, Valencia, Calif.). .. The enzymatic and mechanical disruption protocol provided by the manufacturer was used, with the exception that cell lysis was performed with sonication at 21W three times for 20 s each (with cells iced between bursts) instead of bead beating.

    Cellular Antioxidant Activity Assay:

    Article Title: Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases
    Article Snippet: Strain JM39 was cultivated in LB medium or LB plus 10 mM l -cysteine, and total RNA was prepared using an RNeasy Protect Bacteria Mini kit (QIAGEN, Valencia, Calif.). .. As a DNA probe, the DNA fragment of tnaA was prepared by PCR with oligonucleotide primers 5′-CCG TTC CGC ATT CGT GTT AT-3′ and 5′-TGC GGT GAA GTG ACG CAA TA-3′.

    RNA Sequencing Assay:

    Article Title: Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival
    Article Snippet: Paragraph title: Illumina RNA sequencing ... Total RNA was prepared using the RNeasy Protect Bacteria Mini Kit (QIAGEN Biotech Co. Ltd., Hilden, Germany) and QIAGEN RNeasy Mini Kit (QIAGEN) according to the manufacturer’s protocols.

    Isolation:

    Article Title: An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death
    Article Snippet: Paragraph title: Isolation of genomic DNA and total RNA ... Cells were pelleted by centrifugation and RNA was extracted according to the RNeasy Protect Bacteria Mini Kit protocol (QIAGEN) with a minor modification, namely DNase digestion was performed on-column for 30 min at 37°C.

    Article Title: Characterization of the MSMEG_2631 Gene (mmp) Encoding a Multidrug and Toxic Compound Extrusion (MATE) Family Protein in Mycobacterium smegmatis and Exploration of Its Polyspecific Nature Using Biolog Phenotype MicroArray
    Article Snippet: Total RNA was isolated from 7H9-grown, mid-exponential-phase M. smegmatis (OD600 nm , ca. .. 0.8 to 0.9) using the RNeasy Protect Bacteria Mini Kit (Qiagen).

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: .. We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions. .. To extract bacterial RNA from infected plants we used a protocol for extracting RNA from P.syringae recovered from infected leaves as described by Yu et al .

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: .. Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , . .. The transcript containing the 3′ end of nlpD and the 5′ end of penA was amplified using Q5 High-Fidelity Polymerase according to the manufacturer’s recommendations using primers P2999 & P3003.

    Article Title: Listeria monocytogenes ?B Regulates Stress Response and Virulence Functions
    Article Snippet: Paragraph title: DNA and RNA isolation and treatment. ... RNA was extracted from L. monocytogenes either grown to stationary phase or exposed to osmotic stress (as described below) with the RNeasy Protect Bacteria midi kit (Qiagen, Valencia, Calif.).

    Polymerase Chain Reaction:

    Article Title: Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases
    Article Snippet: Strain JM39 was cultivated in LB medium or LB plus 10 mM l -cysteine, and total RNA was prepared using an RNeasy Protect Bacteria Mini kit (QIAGEN, Valencia, Calif.). .. As a DNA probe, the DNA fragment of tnaA was prepared by PCR with oligonucleotide primers 5′-CCG TTC CGC ATT CGT GTT AT-3′ and 5′-TGC GGT GAA GTG ACG CAA TA-3′.

    Article Title: Characterization of the MSMEG_2631 Gene (mmp) Encoding a Multidrug and Toxic Compound Extrusion (MATE) Family Protein in Mycobacterium smegmatis and Exploration of Its Polyspecific Nature Using Biolog Phenotype MicroArray
    Article Snippet: 0.8 to 0.9) using the RNeasy Protect Bacteria Mini Kit (Qiagen). .. Reverse transcriptase PCR (RT-PCR) was carried out with 0.3 to 0.5 μg RNA using a one-step RT-PCR kit (Qiagen).

    RNA Extraction:

    Article Title: Arrangement of the Clostridium baratii F7 Toxin Gene Cluster with Identification of a ? Factor That Recognizes the Botulinum Toxin Gene Cluster Promoters
    Article Snippet: .. RNA extraction The bacteria were grown as described above until early stationary phase (OD600 ∼1.3), at which point RNA was extracted with the RNeasy Protect Bacteria Mini Kit (Qiagen) that included treatment with DNase. .. Rapid Amplification of 5' Complementary DNA Ends (5' RACE) The transcription start sites of the bont gene cluster mRNA transcripts were mapped with the 5′ RACE System for Rapid Amplification of cDNA Ends, Version 2.0 (Invitrogen).

    Article Title: Characterization of the MSMEG_2631 Gene (mmp) Encoding a Multidrug and Toxic Compound Extrusion (MATE) Family Protein in Mycobacterium smegmatis and Exploration of Its Polyspecific Nature Using Biolog Phenotype MicroArray
    Article Snippet: Paragraph title: RNA extraction and RT-PCR. ... 0.8 to 0.9) using the RNeasy Protect Bacteria Mini Kit (Qiagen).

    Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation
    Article Snippet: .. RNA extraction and quantitative real-time polymerase chain reaction (qPCR) Total RNA of A . baumannii was extracted using an RNeasy Protect Bacteria Mini kit (Qiagen, Tokyo, Japan). .. To analyze the efflux pump and biofilm-related gene expression levels in A . baumannii , total RNA was extracted from the bacteria after 16 hours of incubation.

    Article Title: Expression of the Biofilm-Associated Genes in Methicillin-Resistant Staphylococcus aureus in Biofilm and Planktonic Conditions
    Article Snippet: .. RNA Extraction and cDNA Synthesis RNA was extracted from lysates of bacterial cells using the RNeasy Protect Bacteria Kit (QIAGEN), according to the manufacturer’s instructions. .. Quantification of RNA was conducted using an Epoch microplate spectrophotometer (BioTek Instruments, Inc., Winooski, VT, USA).

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: Paragraph title: Bacterial RNA extraction and cDNA synthesis ... We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions.

    Article Title: Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans
    Article Snippet: Paragraph title: 3.2. Culture at Separate Growth Phases and RNA Extraction ... The cultures were grown with shaking at 180 rpm to an OD600nm of 0.8, which corresponds to the early stationary growth phase , and then stabilized by treatment with RNA Protect (RNeasy Protect Bacteria Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s recommendations.

    Labeling:

    Article Title: Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases
    Article Snippet: Northern blot analysis for tnaA was carried out by using a Gene Images Random-Prime Labeling and Detection System (Amersham Pharmacia Biotech, Buckinghamshire, England). .. Strain JM39 was cultivated in LB medium or LB plus 10 mM l -cysteine, and total RNA was prepared using an RNeasy Protect Bacteria Mini kit (QIAGEN, Valencia, Calif.).

    Purification:

    Article Title: Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans
    Article Snippet: The cultures were grown with shaking at 180 rpm to an OD600nm of 0.8, which corresponds to the early stationary growth phase , and then stabilized by treatment with RNA Protect (RNeasy Protect Bacteria Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s recommendations. .. RNA was purified using the RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s protocol for enzymatic lysis and the proteinase K digestion of bacteria, with the lysis time extended to 30 minutes.

    Sequencing:

    Article Title: Quorum sensing-regulated chitin metabolism provides grazing resistance to Vibrio cholerae biofilms
    Article Snippet: .. After removing the planktonic phase, the total RNA was extracted from biofilms formed on chitin flakes using the RNeasy Protect Bacteria Mini Kit (Qiagen, Hilden, Germany) as per the manufacturer's recommendation, and paired-end sequencing was performed on the Illumina Hi-Seq 2000 platform (San Diego, CA, USA) with 237 million reads of 100 bp length. .. Reads from Illumina were processed with Cutadapt (version 1.3) ( ) with a quality trimming threshold of 20 and a minimum read length of 50 bp.

    Software:

    Article Title: Molecular Investigations of PenA-mediated ?-lactam Resistance in Burkholderia pseudomallei
    Article Snippet: Quantification of penA transcript levels Overnight cultures were subcultured into LB medium, grown to an OD600 nm of 0.5 and RNA was extracted with the RNeasy Protect Bacteria Mini Kit (Qiagen, Valencia, CA, USA). cDNA was synthesized using the SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen) and quantified using SYBR GreenER qPCR SuperMix for iCycler Instruments (Invitrogen) and the Bio-Rad iQ5 iCycler. .. Data analyses were performed using the iCycler software.

    Negative Control:

    Article Title: Transcriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
    Article Snippet: Total RNA was isolated using the RNeasy Protect Bacteria mini kit (Qiagen, Valencia, CA) and cDNA synthesis was performed as previously described , . .. Genomic DNA isolated from 1026b cells using the PureGene Core kit A (QIAGEN, Valencia, CA) was used as a positive control reaction, while DNAse treated cDNA generated from 1026b and Bp1651 RNA and sterile water were used as negative control templates.

    In Vitro:

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: For RNA isolation from in vitro cultured bacteria, Pst DC3000 was grown on KB agar plates for 48 h at 28°C. .. We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions.

    Incubation:

    Article Title: An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death
    Article Snippet: For isolation of total RNA, E. coli DH5α cells were cultured in LB medium to an OD600 of 0.7, mixed with RNAprotect Bacteria Reagent (QIAGEN) by vortexing followed by incubation for 5 min. at room temperature. .. Cells were pelleted by centrifugation and RNA was extracted according to the RNeasy Protect Bacteria Mini Kit protocol (QIAGEN) with a minor modification, namely DNase digestion was performed on-column for 30 min at 37°C.

    Article Title: Characterization of the MSMEG_2631 Gene (mmp) Encoding a Multidrug and Toxic Compound Extrusion (MATE) Family Protein in Mycobacterium smegmatis and Exploration of Its Polyspecific Nature Using Biolog Phenotype MicroArray
    Article Snippet: 0.8 to 0.9) using the RNeasy Protect Bacteria Mini Kit (Qiagen). .. Cycling conditions were 52°C for 30 min, 95°C for 15 min, 30 cycles of 95°C for 30 s, 56 to 60°C (according to the primer) for 30 s, and 72°C for 1 min, followed by incubation at 72°C for 10 min. For two-step RT-PCR, RNA samples were reverse transcribed using the AffinityScript Multiple Temperature cDNA synthesis kit (Agilent Technologies) with template cDNA with PCR primer sets.

    Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qPCR) Total RNA of A . baumannii was extracted using an RNeasy Protect Bacteria Mini kit (Qiagen, Tokyo, Japan). .. To analyze the efflux pump and biofilm-related gene expression levels in A . baumannii , total RNA was extracted from the bacteria after 16 hours of incubation.

    Article Title: Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans
    Article Snippet: Culture at Separate Growth Phases and RNA Extraction Planktonic cultures were generated by overnight incubation with shaking at 180 rpm of a single colony transferred to 10 ml of BHI media. .. The cultures were grown with shaking at 180 rpm to an OD600nm of 0.8, which corresponds to the early stationary growth phase , and then stabilized by treatment with RNA Protect (RNeasy Protect Bacteria Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s recommendations.

    Spectrophotometry:

    Article Title: Sub-minimum inhibitory concentrations of colistin and polymyxin B promote Acinetobacter baumannii biofilm formation
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qPCR) Total RNA of A . baumannii was extracted using an RNeasy Protect Bacteria Mini kit (Qiagen, Tokyo, Japan). .. Harvested RNA samples were quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific, MA, USA).

    Article Title: Expression of the Biofilm-Associated Genes in Methicillin-Resistant Staphylococcus aureus in Biofilm and Planktonic Conditions
    Article Snippet: RNA Extraction and cDNA Synthesis RNA was extracted from lysates of bacterial cells using the RNeasy Protect Bacteria Kit (QIAGEN), according to the manufacturer’s instructions. .. Quantification of RNA was conducted using an Epoch microplate spectrophotometer (BioTek Instruments, Inc., Winooski, VT, USA).

    Article Title: Resistance Inducers Modulate Pseudomonas syringae pv. Tomato Strain DC3000 Response in Tomato Plants
    Article Snippet: We used the Qiagen RNeasy Bacteria Mini Kit for isolation of RNA samples according to manufacturer's instructions. .. RNA quantification was performed with a UV-VIS UNICAM Model Heλiosβ spectrophotometer.

    Lysis:

    Article Title: Motility, Biofilm Formation and Antimicrobial Efflux of Sessile and Planktonic Cells of Achromobacter xylosoxidans
    Article Snippet: The cultures were grown with shaking at 180 rpm to an OD600nm of 0.8, which corresponds to the early stationary growth phase , and then stabilized by treatment with RNA Protect (RNeasy Protect Bacteria Mini Kit, Qiagen, Hilden, Germany) according to the manufacturer’s recommendations. .. RNA was purified using the RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s protocol for enzymatic lysis and the proteinase K digestion of bacteria, with the lysis time extended to 30 minutes.

    Article Title: Listeria monocytogenes ?B Regulates Stress Response and Virulence Functions
    Article Snippet: RNA was extracted from L. monocytogenes either grown to stationary phase or exposed to osmotic stress (as described below) with the RNeasy Protect Bacteria midi kit (Qiagen, Valencia, Calif.). .. The enzymatic and mechanical disruption protocol provided by the manufacturer was used, with the exception that cell lysis was performed with sonication at 21W three times for 20 s each (with cells iced between bursts) instead of bead beating.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen rneasy protect bacteria minikit
    Rneasy Protect Bacteria Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy protect bacteria minikit/product/Qiagen
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rneasy protect bacteria minikit - by Bioz Stars, 2020-03
    99/100 stars
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    99
    Qiagen rneasy minikit
    Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minikit/product/Qiagen
    Average 99 stars, based on 2156 article reviews
    Price from $9.99 to $1999.99
    rneasy minikit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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