rneasy plus universal minikit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    RNeasy Plus Universal Mini Kit
    Description:
    For purification of total RNA from all types of tissue using gDNA Eliminator Solution Kit contents Qiagen RNeasy Plus Universal Mini Kit 50 preps 50 to 250mg Sample 150L Elution Volume Mini Format QIAzol Spin Column Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Array Analysis Next generation Sequencing For Purification of Total RNA using gDNA Eliminator Solution Includes RNeasy Mini Spin Columns gDNA Eliminator Solution Collection Tubes RNase free Water and Buffers Benefits One for all tissue solution Time saving integration of RNeasy and QIAzol technologies Fast non enzymatic removal of genomic DNA Pure RNA for use in downstream applicatio
    Catalog Number:
    73404
    Price:
    406
    Category:
    RNeasy Plus Universal Kits
    Buy from Supplier


    Structured Review

    Qiagen rneasy plus universal minikit
    RNeasy Plus Universal Mini Kit
    For purification of total RNA from all types of tissue using gDNA Eliminator Solution Kit contents Qiagen RNeasy Plus Universal Mini Kit 50 preps 50 to 250mg Sample 150L Elution Volume Mini Format QIAzol Spin Column Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Array Analysis Next generation Sequencing For Purification of Total RNA using gDNA Eliminator Solution Includes RNeasy Mini Spin Columns gDNA Eliminator Solution Collection Tubes RNase free Water and Buffers Benefits One for all tissue solution Time saving integration of RNeasy and QIAzol technologies Fast non enzymatic removal of genomic DNA Pure RNA for use in downstream applicatio
    https://www.bioz.com/result/rneasy plus universal minikit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rneasy plus universal minikit - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa). .. The amplification products from primer sets f1+f3 and f2+r2 were purified and sequenced.

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction. .. However, if yield is not a critical issue (e.g. cDNA synthesis for PCR amplification) or amplification and cleanup of pooled samples is part of a design, then the convenient Macherey–Nagel NuleoSpin Plant or Qiagen RNeasy Plant kit are attractive choices.

    Synthesized:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Total RNA was extracted using RNeasy Plus Universal kit (Qiagen). .. RNA integrity and quantity was checked using the LabChip GX instrument (Perkin Elmer) and the quality score was > 9.0 for all samples. cDNA was synthesized using SuperScript VILO kit and random hexamers (Life Technologies).

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: .. Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction. ..

    Article Title: Characterizing Newly Repopulated Microglia in the Adult Mouse: Impacts on Animal Behavior, Cell Morphology, and Neuroinflammation
    Article Snippet: .. RNA was extracted (RNA Plus Universal Mini Kit, Qiagen), cDNA synthesized (iScript kit, BioRad), and quantitative RT-PCR performed using a commercially available immune panel that tests 96 genes (including pro-inflammatory cytokines, chemokines, and microglial specific markers; Taqman Array Mouse Immune Panel; Invitrogen). mRNA expression data were analyzed using the comparative threshold cycle (Ct) method [ ] and results are expressed as percent change from controls administered PBS. .. Statistical Analysis Data were analyzed using unpaired student’s t-tests (control vs. repopulated) in Microsoft Excel or as a two-way ANOVA (control vs. repopulated and LPS vs. PBS, with “mouse” as the experimental unit) using the MIXED procedure of the Statistical Analysis Systems software (SAS Institute Inc.).

    Cytometry:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Purity of selected fractions monitored by flow cytometry showed that > 85% of cells expressed the selected marker with < 1% CD14+ cells in non-monocyte fractions. .. Total RNA was extracted using RNeasy Plus Universal kit (Qiagen).

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: Analysis of cis-regulatory effects of individual 3' UTR sequences To analyze effects of individual full-length 3' UTRs or 3' UTR segments, we harvested transduced cells 72 h after infection, stained cells with Alexa647-conjugated ME20-4 anti-LNGFR antibody, fixed cells with 1% paraformaldehyde, and analyzed cells using a FACSCanto flow cytometer (Becton Dickinson). .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript).

    Quantitative RT-PCR:

    Article Title: The evolution of a series of behavioral traits is associated with autism-risk genes in cavefish
    Article Snippet: The total RNA extraction was performed by using the RNeasy Plus Universal kit (Qiagen) with an elution volume of 20 μl. .. RNA quality and quantity were determined based on electrophoresis and Qubit 3.0 Fluorometer system (Thermo Fisher Scientific, Waltham, MA), respectively. iScript gDNA Clear cDNA Synthesis kit was used to eliminate the carryover of genomic DNA, followed by synthesis of cDNA by using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories, Hercules, CA).

    Article Title: Characterizing Newly Repopulated Microglia in the Adult Mouse: Impacts on Animal Behavior, Cell Morphology, and Neuroinflammation
    Article Snippet: .. RNA was extracted (RNA Plus Universal Mini Kit, Qiagen), cDNA synthesized (iScript kit, BioRad), and quantitative RT-PCR performed using a commercially available immune panel that tests 96 genes (including pro-inflammatory cytokines, chemokines, and microglial specific markers; Taqman Array Mouse Immune Panel; Invitrogen). mRNA expression data were analyzed using the comparative threshold cycle (Ct) method [ ] and results are expressed as percent change from controls administered PBS. .. Statistical Analysis Data were analyzed using unpaired student’s t-tests (control vs. repopulated) in Microsoft Excel or as a two-way ANOVA (control vs. repopulated and LPS vs. PBS, with “mouse” as the experimental unit) using the MIXED procedure of the Statistical Analysis Systems software (SAS Institute Inc.).

    Article Title: VEPH1 expression decreases vascularisation in ovarian cancer xenografts and inhibits VEGFA and IL8 expression through inhibition of AKT activation
    Article Snippet: .. Real-time quantitative RT-PCR (qPCR) Total RNA was extracted using Trizol and DNase-treated using a Turbo DNA-free kit (Invitrogen), or RNeasy Plus Universal kit (Qiagen, Toronto, ON, Canada) and reverse-transcribed using random hexamers (Invitrogen). .. Real-time qPCR was performed using SYBR green in an ABI PRISM 7900HT Sequence Detection System using primers (for VEPH1 , Interleukin 8 (IL8 ), glyceraldehyde 3-phosphate dehydrogena se (GAPDH ) or TATA binding protein (TBP )) and conditions previously described ( ; ).

    Real-time Polymerase Chain Reaction:

    Article Title: Toll-like receptor 3 as an immunotherapeutic target for KRAS mutated colorectal cancer
    Article Snippet: Paragraph title: Quantitative real time PCR to measure gene expression of TLR3 and TRIF and Reovirus Lambda 3 ... Essentially 500ng of RNA (Qiagen RNAeasy mini kit# 74104) was isolated from HCT116 cells harvested from each culture condition (control and reovirus-treated) at 24 hours and was used to make cDNA with Iscript cDNA synthesizing kit (Bio-Rad #1708890).

    Article Title: The evolution of a series of behavioral traits is associated with autism-risk genes in cavefish
    Article Snippet: Paragraph title: Quantitative PCR ... The total RNA extraction was performed by using the RNeasy Plus Universal kit (Qiagen) with an elution volume of 20 μl.

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript). ..

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction. .. Q-RT-PCR analysis was performed in the iCycler iQ Multi-Color Real Time PCR Detection System (Bio-Rad). cDNA amounts present in each sample were determined using iQ SYBR Green Supermix (Bio-Rad).

    Article Title: Lyplal1 is dispensable for normal fat deposition in mice
    Article Snippet: .. RNA extraction and qPCR RNA was extracted from snap-frozen mouse tissue using the Qiagen RNeasy plus Universal kit according to the manufacturer's instructions (Qiagen, Hilden, Germany). ..

    Article Title: Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche
    Article Snippet: QPCR Cells were scraped into 250 μl/well of Trizol, homogenized five times through a 22 g needle and five times through a 25 g needle. .. Genomic DNA was digested with 50 ul gDNase solution (RNeasy Plus Universal Kit, Qiagen).

    Article Title: VEPH1 expression decreases vascularisation in ovarian cancer xenografts and inhibits VEGFA and IL8 expression through inhibition of AKT activation
    Article Snippet: .. Real-time quantitative RT-PCR (qPCR) Total RNA was extracted using Trizol and DNase-treated using a Turbo DNA-free kit (Invitrogen), or RNeasy Plus Universal kit (Qiagen, Toronto, ON, Canada) and reverse-transcribed using random hexamers (Invitrogen). .. Real-time qPCR was performed using SYBR green in an ABI PRISM 7900HT Sequence Detection System using primers (for VEPH1 , Interleukin 8 (IL8 ), glyceraldehyde 3-phosphate dehydrogena se (GAPDH ) or TATA binding protein (TBP )) and conditions previously described ( ; ).

    Article Title: Role of the central nervous system and adipose tissue BDNF/TrkB axes in metabolic regulation
    Article Snippet: RNA analysis Total RNA was extracted from adipose tissue by using an RNeasy Plus Universal kit (Qiagen, Valencia, CA, USA) and was extracted from other tissues with RNA-Bee (Molecular Research Center, Cincinnati, OH, USA). .. Then reverse transcription was performed by using a QuantiTect reverse transcription kit (Qiagen), and real-time PCR was done with a LightCycler (Roche), the Taqman Universal Probe Library, and Light Cycler Master (Roche) according to the manufacturer’s instructions.

    Activity Assay:

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: We eliminated intensive preparation of spatulas and mortars and pestles by baking at 200 °C prior to use because native plant RNase activity [ ] renders these steps redundant due to the processing of tissues with liquid N2 and the strong denaturing conditions of lysis buffers. .. While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction.

    Cell Culture:

    Article Title: CCR6, the Sole Receptor for the Chemokine CCL20, Promotes Spontaneous Intestinal Tumorigenesis
    Article Snippet: .. RNA extraction and semi-quantitative RT-PCR Total RNA was extracted either from tumor tissue of colorectal cancer patients or from cultured MC38, HT29 or Hct116 cells using the RNAeasy Plus Universal Kit (Qiagen, Valencia, CA). .. Before extracting total RNA, cell lysates were prepared from 100 mg of tumor tissue samples by mixing with 900 ml of QIAzol Lysis Reagent (Qiagen) in an Eppendorf tube followed by homogenization using the Tissue Lyser II (Qiagen).

    Article Title: Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche
    Article Snippet: Genomic DNA was digested with 50 ul gDNase solution (RNeasy Plus Universal Kit, Qiagen). .. Data were normalized to beta-actin , analyzed using ΔΔCT method, and expressed as mean ±s.d relative to Bmp2F/F cells cultured in osteogenic medium.

    Expressing:

    Article Title: Toll-like receptor 3 as an immunotherapeutic target for KRAS mutated colorectal cancer
    Article Snippet: Paragraph title: Quantitative real time PCR to measure gene expression of TLR3 and TRIF and Reovirus Lambda 3 ... Essentially 500ng of RNA (Qiagen RNAeasy mini kit# 74104) was isolated from HCT116 cells harvested from each culture condition (control and reovirus-treated) at 24 hours and was used to make cDNA with Iscript cDNA synthesizing kit (Bio-Rad #1708890).

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Paragraph title: Analysis of mRNA expression ... Total RNA was extracted using RNeasy Plus Universal kit (Qiagen).

    Article Title: Characterizing Newly Repopulated Microglia in the Adult Mouse: Impacts on Animal Behavior, Cell Morphology, and Neuroinflammation
    Article Snippet: .. RNA was extracted (RNA Plus Universal Mini Kit, Qiagen), cDNA synthesized (iScript kit, BioRad), and quantitative RT-PCR performed using a commercially available immune panel that tests 96 genes (including pro-inflammatory cytokines, chemokines, and microglial specific markers; Taqman Array Mouse Immune Panel; Invitrogen). mRNA expression data were analyzed using the comparative threshold cycle (Ct) method [ ] and results are expressed as percent change from controls administered PBS. .. Statistical Analysis Data were analyzed using unpaired student’s t-tests (control vs. repopulated) in Microsoft Excel or as a two-way ANOVA (control vs. repopulated and LPS vs. PBS, with “mouse” as the experimental unit) using the MIXED procedure of the Statistical Analysis Systems software (SAS Institute Inc.).

    Article Title: Role of the central nervous system and adipose tissue BDNF/TrkB axes in metabolic regulation
    Article Snippet: RNA analysis Total RNA was extracted from adipose tissue by using an RNeasy Plus Universal kit (Qiagen, Valencia, CA, USA) and was extracted from other tissues with RNA-Bee (Molecular Research Center, Cincinnati, OH, USA). .. For normalization of gene expression, 36B4 mRNA was used as the internal control.

    Electron Microscopy:

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Using the Palkovits punch technique (Electron Microscopy Science, PA), PVH was dissected using the fornix, optic tracts, and third ventricle as landmarks (Bregma, around −0.22mm to −1.70mm). .. Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction.

    Flow Cytometry:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Purity of selected fractions monitored by flow cytometry showed that > 85% of cells expressed the selected marker with < 1% CD14+ cells in non-monocyte fractions. .. Total RNA was extracted using RNeasy Plus Universal kit (Qiagen).

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: Flow cytometric analyses were performed in triplicate. .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript).

    Dissection:

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Whole hypothalamus was isolated by block dissection according as previously described. .. Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction.

    Infection:

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: Analysis of cis-regulatory effects of individual 3' UTR sequences To analyze effects of individual full-length 3' UTRs or 3' UTR segments, we harvested transduced cells 72 h after infection, stained cells with Alexa647-conjugated ME20-4 anti-LNGFR antibody, fixed cells with 1% paraformaldehyde, and analyzed cells using a FACSCanto flow cytometer (Becton Dickinson). .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript).

    Polymerase Chain Reaction:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Total RNA was extracted using RNeasy Plus Universal kit (Qiagen). .. The primers and PCR conditions were the same as those used in the SYBR Green CNV genotyping method described above.

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa). .. PCR was performed with PrimeSTAR DNA polymerase (TaKaRa) using the following primers: f1, 5′-GGCTGAGGCTGAGTCAGGATTGG-3′; f2, 5′-CGTCCTTACGGCACAAGTCAGTTACC-3′; r1, 5′-GAGGCTACAACAGGAGTTGTAAAGGC-3′; r2, 5′-GAGTCATCACAGCAACCAGAGCGTG-3′; r3, 5′-GCGGGCTGGGCTTCGTGCCAAGG-3′; r4, 5′-CTGGGAATAGACCTTGCTGAGCCGT-3′; r5, 5′-GGCGGCTTGGAGACATGAGGCCTGAT-3′.

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction. .. However, if yield is not a critical issue (e.g. cDNA synthesis for PCR amplification) or amplification and cleanup of pooled samples is part of a design, then the convenient Macherey–Nagel NuleoSpin Plant or Qiagen RNeasy Plant kit are attractive choices.

    Sequencing:

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: We normalized the median ratio of GFP/LNGFR fluorescence for transduced (LNGFR-positive) cells relative to ratios obtained using the empty BTV reporter (no 3' UTR test sequence inserted, defined as 100%) and a version of the reporter lacking the GFP transgene (defined as 0%). .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript).

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction. .. The CTAB method is more scalable, though given input requirements for modern applications such as transcriptome sequencing (i.e. Illumina TruSeq Stranded mRNA— http://www.illumina.com ), preparations yielding as little as 25 ng of total RNA may be sufficient.

    Article Title: VEPH1 expression decreases vascularisation in ovarian cancer xenografts and inhibits VEGFA and IL8 expression through inhibition of AKT activation
    Article Snippet: Real-time quantitative RT-PCR (qPCR) Total RNA was extracted using Trizol and DNase-treated using a Turbo DNA-free kit (Invitrogen), or RNeasy Plus Universal kit (Qiagen, Toronto, ON, Canada) and reverse-transcribed using random hexamers (Invitrogen). .. Real-time qPCR was performed using SYBR green in an ABI PRISM 7900HT Sequence Detection System using primers (for VEPH1 , Interleukin 8 (IL8 ), glyceraldehyde 3-phosphate dehydrogena se (GAPDH ) or TATA binding protein (TBP )) and conditions previously described ( ; ).

    Injection:

    Article Title: Characterizing Newly Repopulated Microglia in the Adult Mouse: Impacts on Animal Behavior, Cell Morphology, and Neuroinflammation
    Article Snippet: Inflammatory Profiling For inflammatory profiling of the microglia repopulated brain, half of the mice from each dietary treatment (control or 21 d PLX3397) were administered either LPS (0.25 mg/kg) or an equivalent volume of PBS via IP injection (n = 5 per group). .. RNA was extracted (RNA Plus Universal Mini Kit, Qiagen), cDNA synthesized (iScript kit, BioRad), and quantitative RT-PCR performed using a commercially available immune panel that tests 96 genes (including pro-inflammatory cytokines, chemokines, and microglial specific markers; Taqman Array Mouse Immune Panel; Invitrogen). mRNA expression data were analyzed using the comparative threshold cycle (Ct) method [ ] and results are expressed as percent change from controls administered PBS.

    Recombinant:

    Article Title: Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche
    Article Snippet: Confluent cultures were stimulated on day one with Osteogenic Medium (OM: BMSC medium plus 50 μg/ml ascorbic acid and 10 mM β-glycerophosphate)±200 ng/ml rhBMP2 (Genetics Institute) or 40 ng/ml recombinant mouse Wnt3a (R and D Systems). .. Genomic DNA was digested with 50 ul gDNase solution (RNeasy Plus Universal Kit, Qiagen).

    Fluorescence:

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: We normalized the median ratio of GFP/LNGFR fluorescence for transduced (LNGFR-positive) cells relative to ratios obtained using the empty BTV reporter (no 3' UTR test sequence inserted, defined as 100%) and a version of the reporter lacking the GFP transgene (defined as 0%). .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript).

    Mutagenesis:

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: .. To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa). .. PCR was performed with PrimeSTAR DNA polymerase (TaKaRa) using the following primers: f1, 5′-GGCTGAGGCTGAGTCAGGATTGG-3′; f2, 5′-CGTCCTTACGGCACAAGTCAGTTACC-3′; r1, 5′-GAGGCTACAACAGGAGTTGTAAAGGC-3′; r2, 5′-GAGTCATCACAGCAACCAGAGCGTG-3′; r3, 5′-GCGGGCTGGGCTTCGTGCCAAGG-3′; r4, 5′-CTGGGAATAGACCTTGCTGAGCCGT-3′; r5, 5′-GGCGGCTTGGAGACATGAGGCCTGAT-3′.

    Isolation:

    Article Title: Toll-like receptor 3 as an immunotherapeutic target for KRAS mutated colorectal cancer
    Article Snippet: .. Essentially 500ng of RNA (Qiagen RNAeasy mini kit# 74104) was isolated from HCT116 cells harvested from each culture condition (control and reovirus-treated) at 24 hours and was used to make cDNA with Iscript cDNA synthesizing kit (Bio-Rad #1708890). .. Real time PCR was performed in 96 well qPCR plate using Affymetrix VeriQuest Fast SYBR Green qPCR Master Mix (2X) (Affymetrix part #75690) as fluorescent dye for detection.

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: PBMCs were isolated using a Ficoll technique and cell fractions were obtained by negative selection for CD8 and CD56, and positive selection for CD4, CD14, and CD19 using EasySep magnetic platform (StemCell Technologies). .. Total RNA was extracted using RNeasy Plus Universal kit (Qiagen).

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Whole hypothalamus was isolated by block dissection according as previously described. .. Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction.

    Article Title: Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle
    Article Snippet: Paragraph title: RNA isolation and purification ... RNA was subsequently precipitated and purified using the RNeasy Plus Universal kit (Qiagen, UK) according to the manufacturer’s guidelines, which included a step to remove any contaminating genomic DNA.

    Purification:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Total RNA was extracted using RNeasy Plus Universal kit (Qiagen). .. The equimolar mix of the PCR fragments products was prepared by purification of the PCR products using QIAquick PCR purification kit (Qiagen) and quantification using the LabChip GX (Perkin Elmer).

    Article Title: Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle
    Article Snippet: .. RNA was subsequently precipitated and purified using the RNeasy Plus Universal kit (Qiagen, UK) according to the manufacturer’s guidelines, which included a step to remove any contaminating genomic DNA. .. The quantity of the RNA isolated was determined by measuring the absorbance at 260 nm using a Nanodrop spectrophotometer ND-1000 (Nanodrop Technologies, Wilmington, DE, USA).

    Article Title: CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration . CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
    Article Snippet: .. After obtaining muscle sections for histology, the remainder of the muscle sample (32 ± 1.2 mg) was mechanically disrupted and homogenized using a plastic pestle in a 1.5‐mL microcentrifuge tube with 900 μ L of QIAzol Lysis Reagent (QIAGEN, Hilden, Germany) and RNA was purified using a RNeasy Plus Universal kit (QIAGEN) according to the protocol provided in the kit. .. Muscle histology and immunohistochemistry To observe general muscle architecture and measure muscle fiber cross‐sectional area (CSA), samples were stained using hemotoxylin and eosin (H & E).

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa). .. The amplification products from primer sets f1+f3 and f2+r2 were purified and sequenced.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Toll-like receptor 3 as an immunotherapeutic target for KRAS mutated colorectal cancer
    Article Snippet: Essentially 500ng of RNA (Qiagen RNAeasy mini kit# 74104) was isolated from HCT116 cells harvested from each culture condition (control and reovirus-treated) at 24 hours and was used to make cDNA with Iscript cDNA synthesizing kit (Bio-Rad #1708890). .. Bio-Rad CFX96 RT-PCR machine was used to amplify the cDNA.

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: Paragraph title: RNA Extraction and RT-PCR. ... To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa).

    Article Title: CCR6, the Sole Receptor for the Chemokine CCL20, Promotes Spontaneous Intestinal Tumorigenesis
    Article Snippet: .. RNA extraction and semi-quantitative RT-PCR Total RNA was extracted either from tumor tissue of colorectal cancer patients or from cultured MC38, HT29 or Hct116 cells using the RNAeasy Plus Universal Kit (Qiagen, Valencia, CA). .. Before extracting total RNA, cell lysates were prepared from 100 mg of tumor tissue samples by mixing with 900 ml of QIAzol Lysis Reagent (Qiagen) in an Eppendorf tube followed by homogenization using the Tissue Lyser II (Qiagen).

    Selection:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Total PBMCs were used for selection of all fractions with the exception of CD4 T cells, for which we used CD14 monocyte depleted PBMCs. .. Total RNA was extracted using RNeasy Plus Universal kit (Qiagen).

    Blocking Assay:

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Whole hypothalamus was isolated by block dissection according as previously described. .. Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction.

    Staining:

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: Analysis of cis-regulatory effects of individual 3' UTR sequences To analyze effects of individual full-length 3' UTRs or 3' UTR segments, we harvested transduced cells 72 h after infection, stained cells with Alexa647-conjugated ME20-4 anti-LNGFR antibody, fixed cells with 1% paraformaldehyde, and analyzed cells using a FACSCanto flow cytometer (Becton Dickinson). .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript).

    Article Title: Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche
    Article Snippet: Alizarin red Fixed cells were stained for 30 mins in 0.4% aqueous solution of alizarin red S (Sigma). .. Genomic DNA was digested with 50 ul gDNase solution (RNeasy Plus Universal Kit, Qiagen).

    Mouse Assay:

    Article Title: CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration . CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
    Article Snippet: Tissue sampling and preparation At the conclusion of the muscle function assessment, the mice were killed by exsanguination (using vena cava blood draw) followed by cervical dislocation while deeply anesthetized. .. After obtaining muscle sections for histology, the remainder of the muscle sample (32 ± 1.2 mg) was mechanically disrupted and homogenized using a plastic pestle in a 1.5‐mL microcentrifuge tube with 900 μ L of QIAzol Lysis Reagent (QIAGEN, Hilden, Germany) and RNA was purified using a RNeasy Plus Universal kit (QIAGEN) according to the protocol provided in the kit.

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: .. To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa). .. PCR was performed with PrimeSTAR DNA polymerase (TaKaRa) using the following primers: f1, 5′-GGCTGAGGCTGAGTCAGGATTGG-3′; f2, 5′-CGTCCTTACGGCACAAGTCAGTTACC-3′; r1, 5′-GAGGCTACAACAGGAGTTGTAAAGGC-3′; r2, 5′-GAGTCATCACAGCAACCAGAGCGTG-3′; r3, 5′-GCGGGCTGGGCTTCGTGCCAAGG-3′; r4, 5′-CTGGGAATAGACCTTGCTGAGCCGT-3′; r5, 5′-GGCGGCTTGGAGACATGAGGCCTGAT-3′.

    Chromatin Immunoprecipitation:

    Article Title: Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle
    Article Snippet: RNA was subsequently precipitated and purified using the RNeasy Plus Universal kit (Qiagen, UK) according to the manufacturer’s guidelines, which included a step to remove any contaminating genomic DNA. .. RNA quality was assessed on the Agilent Bioanalyser 2100 using the RNA 6000 Nano Lab Chip kit (Agilent Technologies Ireland Ltd., Dublin, Ireland).

    Software:

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction. .. Crh primer sequences were designed using Primer3 software and synthesized by IDT.

    SYBR Green Assay:

    Article Title: Toll-like receptor 3 as an immunotherapeutic target for KRAS mutated colorectal cancer
    Article Snippet: Essentially 500ng of RNA (Qiagen RNAeasy mini kit# 74104) was isolated from HCT116 cells harvested from each culture condition (control and reovirus-treated) at 24 hours and was used to make cDNA with Iscript cDNA synthesizing kit (Bio-Rad #1708890). .. Real time PCR was performed in 96 well qPCR plate using Affymetrix VeriQuest Fast SYBR Green qPCR Master Mix (2X) (Affymetrix part #75690) as fluorescent dye for detection.

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Total RNA was extracted using RNeasy Plus Universal kit (Qiagen). .. The primers and PCR conditions were the same as those used in the SYBR Green CNV genotyping method described above.

    Article Title: Massively parallel functional annotation of 3' untranslated regions
    Article Snippet: .. To analyze effects on reporter mRNA stability, we extracted RNA (Qiagen RNeasy Mini/Midi Kit), reverse transcribed RNA to cDNA (Invitrogen SuperScript III First-Strand Synthesis System), and analyzed cDNA by SYBR green quantitative real-time PCR using primers for GFP and LNGFR (used as a reference transcript). ..

    Article Title: Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice
    Article Snippet: Collected tissues from animals were transferred into RNAlater, and total RNA was extracted using RNeasy Plus Universal kits (Qiagen). cDNAs were synthesized (SuperScriptTM III First-Strand Synthesis System; Invitrogen) according to the manufacturer’s instruction. .. Q-RT-PCR analysis was performed in the iCycler iQ Multi-Color Real Time PCR Detection System (Bio-Rad). cDNA amounts present in each sample were determined using iQ SYBR Green Supermix (Bio-Rad).

    Article Title: Lyplal1 is dispensable for normal fat deposition in mice
    Article Snippet: RNA extraction and qPCR RNA was extracted from snap-frozen mouse tissue using the Qiagen RNeasy plus Universal kit according to the manufacturer's instructions (Qiagen, Hilden, Germany). .. RNA integrity was further assessed using an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). cDNA synthesis was performed using between 500 ng and 1 µg RNA, random primers and Superscript II reverse transcriptase (Life Technologies, Carlsbad, CA, USA). qPCR was performed using Sybr Green (Applied Biosystems, Foster City, CA, USA) and run on an AB7500 qPCR machine (Applied Biosystems).

    Article Title: VEPH1 expression decreases vascularisation in ovarian cancer xenografts and inhibits VEGFA and IL8 expression through inhibition of AKT activation
    Article Snippet: Real-time quantitative RT-PCR (qPCR) Total RNA was extracted using Trizol and DNase-treated using a Turbo DNA-free kit (Invitrogen), or RNeasy Plus Universal kit (Qiagen, Toronto, ON, Canada) and reverse-transcribed using random hexamers (Invitrogen). .. Real-time qPCR was performed using SYBR green in an ABI PRISM 7900HT Sequence Detection System using primers (for VEPH1 , Interleukin 8 (IL8 ), glyceraldehyde 3-phosphate dehydrogena se (GAPDH ) or TATA binding protein (TBP )) and conditions previously described ( ; ).

    RNA Extraction:

    Article Title: The evolution of a series of behavioral traits is associated with autism-risk genes in cavefish
    Article Snippet: .. The total RNA extraction was performed by using the RNeasy Plus Universal kit (Qiagen) with an elution volume of 20 μl. .. RNA quality and quantity were determined based on electrophoresis and Qubit 3.0 Fluorometer system (Thermo Fisher Scientific, Waltham, MA), respectively. iScript gDNA Clear cDNA Synthesis kit was used to eliminate the carryover of genomic DNA, followed by synthesis of cDNA by using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories, Hercules, CA).

    Article Title: CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells
    Article Snippet: Paragraph title: RNA Extraction and RT-PCR. ... To examine Camsap3 mRNA expressed in the Camsap3 mutant mice, total RNA was extracted from brains of P21 WT and Camsap3 mutant mice, using an RNeasy Plus Universal Kit (Qiagen) according to the manufacturer’s protocol. cDNA was transcribed from the RNA thus obtained using oligo dT as a primer with PrimeScript RTase (TaKaRa).

    Article Title: CCR6, the Sole Receptor for the Chemokine CCL20, Promotes Spontaneous Intestinal Tumorigenesis
    Article Snippet: .. RNA extraction and semi-quantitative RT-PCR Total RNA was extracted either from tumor tissue of colorectal cancer patients or from cultured MC38, HT29 or Hct116 cells using the RNAeasy Plus Universal Kit (Qiagen, Valencia, CA). .. Before extracting total RNA, cell lysates were prepared from 100 mg of tumor tissue samples by mixing with 900 ml of QIAzol Lysis Reagent (Qiagen) in an Eppendorf tube followed by homogenization using the Tissue Lyser II (Qiagen).

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: Generally older tissues are more recalcitrant to RNA extraction than younger ones for a variety of reasons including presence of co-extracted secondary metabolites [ ] and in the case of climacteric fruit, increases in substances that tend to co-precipitate with RNA [ – ]. .. While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction.

    Article Title: Lyplal1 is dispensable for normal fat deposition in mice
    Article Snippet: .. RNA extraction and qPCR RNA was extracted from snap-frozen mouse tissue using the Qiagen RNeasy plus Universal kit according to the manufacturer's instructions (Qiagen, Hilden, Germany). ..

    Binding Assay:

    Article Title: VEPH1 expression decreases vascularisation in ovarian cancer xenografts and inhibits VEGFA and IL8 expression through inhibition of AKT activation
    Article Snippet: Real-time quantitative RT-PCR (qPCR) Total RNA was extracted using Trizol and DNase-treated using a Turbo DNA-free kit (Invitrogen), or RNeasy Plus Universal kit (Qiagen, Toronto, ON, Canada) and reverse-transcribed using random hexamers (Invitrogen). .. Real-time qPCR was performed using SYBR green in an ABI PRISM 7900HT Sequence Detection System using primers (for VEPH1 , Interleukin 8 (IL8 ), glyceraldehyde 3-phosphate dehydrogena se (GAPDH ) or TATA binding protein (TBP )) and conditions previously described ( ; ).

    Electrophoresis:

    Article Title: The evolution of a series of behavioral traits is associated with autism-risk genes in cavefish
    Article Snippet: The total RNA extraction was performed by using the RNeasy Plus Universal kit (Qiagen) with an elution volume of 20 μl. .. RNA quality and quantity were determined based on electrophoresis and Qubit 3.0 Fluorometer system (Thermo Fisher Scientific, Waltham, MA), respectively. iScript gDNA Clear cDNA Synthesis kit was used to eliminate the carryover of genomic DNA, followed by synthesis of cDNA by using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories, Hercules, CA).

    Homogenization:

    Article Title: CCR6, the Sole Receptor for the Chemokine CCL20, Promotes Spontaneous Intestinal Tumorigenesis
    Article Snippet: RNA extraction and semi-quantitative RT-PCR Total RNA was extracted either from tumor tissue of colorectal cancer patients or from cultured MC38, HT29 or Hct116 cells using the RNAeasy Plus Universal Kit (Qiagen, Valencia, CA). .. Before extracting total RNA, cell lysates were prepared from 100 mg of tumor tissue samples by mixing with 900 ml of QIAzol Lysis Reagent (Qiagen) in an Eppendorf tube followed by homogenization using the Tissue Lyser II (Qiagen).

    Spectrophotometry:

    Article Title: Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle
    Article Snippet: RNA was subsequently precipitated and purified using the RNeasy Plus Universal kit (Qiagen, UK) according to the manufacturer’s guidelines, which included a step to remove any contaminating genomic DNA. .. The quantity of the RNA isolated was determined by measuring the absorbance at 260 nm using a Nanodrop spectrophotometer ND-1000 (Nanodrop Technologies, Wilmington, DE, USA).

    Sampling:

    Article Title: CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration . CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
    Article Snippet: Paragraph title: Tissue sampling and preparation ... After obtaining muscle sections for histology, the remainder of the muscle sample (32 ± 1.2 mg) was mechanically disrupted and homogenized using a plastic pestle in a 1.5‐mL microcentrifuge tube with 900 μ L of QIAzol Lysis Reagent (QIAGEN, Hilden, Germany) and RNA was purified using a RNeasy Plus Universal kit (QIAGEN) according to the protocol provided in the kit.

    Marker:

    Article Title: Diversity of the human LILRB3/A6 locus encoding a myeloid inhibitory and activating receptor pair
    Article Snippet: Purity of selected fractions monitored by flow cytometry showed that > 85% of cells expressed the selected marker with < 1% CD14+ cells in non-monocyte fractions. .. Total RNA was extracted using RNeasy Plus Universal kit (Qiagen).

    Lysis:

    Article Title: The evolution of a series of behavioral traits is associated with autism-risk genes in cavefish
    Article Snippet: The brains of each were homogenized in 1 ml QIAzol Lysis Reagent (Qiagen, Valencia, CA) by using a Micro Tube Homogenizer System (Wilmad-LabGlass, Vineland, NJ). .. The total RNA extraction was performed by using the RNeasy Plus Universal kit (Qiagen) with an elution volume of 20 μl.

    Article Title: CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration . CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
    Article Snippet: .. After obtaining muscle sections for histology, the remainder of the muscle sample (32 ± 1.2 mg) was mechanically disrupted and homogenized using a plastic pestle in a 1.5‐mL microcentrifuge tube with 900 μ L of QIAzol Lysis Reagent (QIAGEN, Hilden, Germany) and RNA was purified using a RNeasy Plus Universal kit (QIAGEN) according to the protocol provided in the kit. .. Muscle histology and immunohistochemistry To observe general muscle architecture and measure muscle fiber cross‐sectional area (CSA), samples were stained using hemotoxylin and eosin (H & E).

    Article Title: CCR6, the Sole Receptor for the Chemokine CCL20, Promotes Spontaneous Intestinal Tumorigenesis
    Article Snippet: RNA extraction and semi-quantitative RT-PCR Total RNA was extracted either from tumor tissue of colorectal cancer patients or from cultured MC38, HT29 or Hct116 cells using the RNAeasy Plus Universal Kit (Qiagen, Valencia, CA). .. Before extracting total RNA, cell lysates were prepared from 100 mg of tumor tissue samples by mixing with 900 ml of QIAzol Lysis Reagent (Qiagen) in an Eppendorf tube followed by homogenization using the Tissue Lyser II (Qiagen).

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: We eliminated intensive preparation of spatulas and mortars and pestles by baking at 200 °C prior to use because native plant RNase activity [ ] renders these steps redundant due to the processing of tissues with liquid N2 and the strong denaturing conditions of lysis buffers. .. While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction.

    Hood:

    Article Title: A practical examination of RNA isolation methods for European pear (Pyrus communis)
    Article Snippet: Importantly, our CTAB based protocol was time equivalent to the kits that were tested, though more equipment intensive (e.g. requiring a fume hood for chloroform handling, a refrigerated centrifuge and a water bath). .. While we did replace the reducing agent B-ME with the less toxic DTT, verified as a suitable B-ME replacement by Mommaerts et al. [ ], the CTAB method still included more hazardous material compared to most kits tested, excluding the Qiagen RNeasy Plus Universal kit which includes the QiaZol reagent and an organic extraction.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen rneasy plus universal minikit
    Rneasy Plus Universal Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plus universal minikit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rneasy plus universal minikit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results