rneasy lipid tissue minikit  (Qiagen)


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    Name:
    RNeasy Lipid Tissue Mini Kit
    Description:
    For purification of up to 100 µg total RNA from fatty tissues and other types of tissue Kit contents Qiagen RNeasy Lipid Tissue Mini Kit 50 preps 10 to 100mg Sample 30 to 100L Elution Volume Fatty Tissue Sample RNA Purification 2 4 to 10mg Yield Spin Column Format Silica Technology 45 min Time Run Ideal for PCR Real time PCR Microarray Includes 50 RNeasy Mini Spin Columns Collection Tubes 1 5 and 2mL QIAzol Lysis Reagent RNase free Reagents and Buffers Benefits Optimized lysis conditions for fatty tissues and other tissues High yields of total RNA without phenol contamination High quality RNA for all downstream applicatio
    Catalog Number:
    74804
    Price:
    401
    Category:
    RNeasy Lipid Tissue Mini Kit
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    Structured Review

    Qiagen rneasy lipid tissue minikit
    RNeasy Lipid Tissue Mini Kit
    For purification of up to 100 µg total RNA from fatty tissues and other types of tissue Kit contents Qiagen RNeasy Lipid Tissue Mini Kit 50 preps 10 to 100mg Sample 30 to 100L Elution Volume Fatty Tissue Sample RNA Purification 2 4 to 10mg Yield Spin Column Format Silica Technology 45 min Time Run Ideal for PCR Real time PCR Microarray Includes 50 RNeasy Mini Spin Columns Collection Tubes 1 5 and 2mL QIAzol Lysis Reagent RNase free Reagents and Buffers Benefits Optimized lysis conditions for fatty tissues and other tissues High yields of total RNA without phenol contamination High quality RNA for all downstream applicatio
    https://www.bioz.com/result/rneasy lipid tissue minikit/product/Qiagen
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    rneasy lipid tissue minikit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: GENE EXPRESSION PROFILE OF MOUSE WHITE ADIPOSE TISSUE DURING INFLAMMATORY STRESS: AGE-DEPENDENT UPREGULATION OF MAJOR PRO-COAGULANT FACTORS
    Article Snippet: .. All centrifugation steps were performed at 4°C and 450 × g. Total RNA was isolated using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valenica, CA, USA). .. Reverse transcription was performed on 1μg of total RNA from adipose tissue, liver, and kidney and 500ng of RNA from isolated adipocytes and SVF cells, after repurification with RNAqueous (Ambion, Austin, TX, USA), using random primers and TaqMan® reverse transcription reagents (Applied Biosystems, Foster City, CA, USA).

    Amplification:

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. Briefly, biotinylated cRNAs were prepared using the Illumina Amplification Kit (Ambion, Austin, USA) and hybridized to the Sentrix Mouse Ref-8 v.2 Expression BeadChip ( > 24,000 probes) (Illumina, San Diego, CA, USA) for 16~18 h at 58℃, according to the manufacturer's instructions.

    Synthesized:

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations
    Article Snippet: .. Total RNA from adipose tissue was isolated using an RNeasy Lipid Tissue Mini kit (Qiagen, Hilden, Germany), following manufacturer's instructions. cDNA was synthesized from 800 ng total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA). .. Quantitative real-time PCR was performed using the following primer pairs: Vegfc , 5′-GAGGTCAAGGCTTTTGAAGGC-3′ (forward) and 5′-CTGTCCTGGTATTGAGGGTGG-3′ (reverse); adiponectin, 5′-GGAGATGCAGGTCTTCTT-3′ (forward) and 5′-CGAATGGGTACATTGGGAAC-3′ (reverse); and 36b4 , 5′-GGACCCGAGAAGACCTCCTT-3′ (forward) and 5′-GCACATCACTCAGAATTTC-3′ (reverse).

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) templates were synthesized for all mice in the same session from equal amounts of total RNA using iScript Kit (BioRad).

    Quantitative RT-PCR:

    Article Title: Initiation and progression of axonopathy in experimental autoimmune encephalomyelitis
    Article Snippet: Paragraph title: RNA isolation and qRT/PCR ... RNA was isolated using RNeasy Lipid Tissue Mini Kit (QIAGEN, CA) and stored at −80°C. cDNA was prepared using Reaction Ready First Strand cDNA Synthesis kits (SuperArray Bioscience Corp., MD).

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Paragraph title: Quantitative real-time RT-PCR ... Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.

    Article Title: Phytochemical analysis and antioxidant and anticancer activities of mastic gum resin from Pistacia atlantica subspecies kurdica
    Article Snippet: .. qRT-PCR assay RNeasy® lipid tissue mini kit (Qiagen NV, Venlo, the Netherlands) was used to extract total RNA from COLO205 cells prior to conducting qRT-PCR analysis. .. The nanophotometer (Implen GmbH, München, Germany) was used to quantify the RNA before aliquoting and storing at −80°C.

    Article Title: Characterization of Neural Estrogen Signaling and Neurotrophic Changes in the Accelerated Ovarian Failure Mouse Model of Menopause
    Article Snippet: Paragraph title: qRT-PCR preparation and analysis ... Total RNA isolation from the dorsal hippocampus was performed using RNeasy Lipid Tissue Mini kit and QIAcube (QIAGEN) in accordance with the manufacturer's instructions.

    SYBR Green Assay:

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations
    Article Snippet: Total RNA from adipose tissue was isolated using an RNeasy Lipid Tissue Mini kit (Qiagen, Hilden, Germany), following manufacturer's instructions. cDNA was synthesized from 800 ng total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA). .. The PCRs were set by using 20 ng of template cDNA per reaction and FastStart SYBR Green master mix (Roche, Basel, Switzerland), according to the manufacturer's instructions, and each reaction was run in duplicate in a BIO-RAD C1000 Thermal cycler (Bio-Rad, Hercules, CA), according to standardized protocols.

    Article Title: Initiation and progression of axonopathy in experimental autoimmune encephalomyelitis
    Article Snippet: RNA was isolated using RNeasy Lipid Tissue Mini Kit (QIAGEN, CA) and stored at −80°C. cDNA was prepared using Reaction Ready First Strand cDNA Synthesis kits (SuperArray Bioscience Corp., MD). .. Real-time PCR was performed using Mouse Toll Like Receptor Signaling Pathway Microarrays and Real-Time SYBR Green PCR Master Mixes (SuperArray Bioscience Corp., MD).

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. SCDH samples from individual mice were prepared for real-time quantitative PCR with Power SYBR Green master kit (PE Applied Biosystems, Foster City, CA) as directed.

    Article Title: Characterization of Neural Estrogen Signaling and Neurotrophic Changes in the Accelerated Ovarian Failure Mouse Model of Menopause
    Article Snippet: Total RNA isolation from the dorsal hippocampus was performed using RNeasy Lipid Tissue Mini kit and QIAcube (QIAGEN) in accordance with the manufacturer's instructions. .. The qRT-PCR was performed using an Applied Biosystems 7900HT Sequence Detection System v2.3 using the SYBR Green PCR Master Mix.

    Microarray:

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: Paragraph title: Microarray analysis ... Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.

    Article Title: Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats
    Article Snippet: Following removal of the RVLM-enriched tissue punches from −80°C storage, RNA from the tissue samples was extracted and purified using RNeasy Lipid Tissue mini kit (Qiagen) with QIAzol Lysis reagent (Qiagen) according to the manufacturer’s protocol. .. The quality and quantity of the RNA used for microarray analysis were tested with the Agilent Bioanalyzer with RNA 6000 nano kit (Agilent Technologies).

    Random Hexamer Labeling:

    Article Title: Mutant Huntingtin in Glial Cells Exacerbates Neurological Symptoms of Huntington Disease Mice *
    Article Snippet: Total RNA from mouse cortex was isolated using the RNeasy Lipid Tissue Mini kit (Qiagen 74804). .. First-strand cDNA was obtained using the Invitrogen SuperScriptTM First Strand Synthesis System for RT-PCR (11904-018), using a combination of random hexamer and Oligo(dT)12–18 primers.

    Expressing:

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations
    Article Snippet: Expression of adiponectin and Vegfc in organs of control mice and ADN–VEGF-C mice after 0.075 mg/mL doxycycline for 7 days was measured by quantitative real-time PCR. .. Total RNA from adipose tissue was isolated using an RNeasy Lipid Tissue Mini kit (Qiagen, Hilden, Germany), following manufacturer's instructions. cDNA was synthesized from 800 ng total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA).

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. Briefly, biotinylated cRNAs were prepared using the Illumina Amplification Kit (Ambion, Austin, USA) and hybridized to the Sentrix Mouse Ref-8 v.2 Expression BeadChip ( > 24,000 probes) (Illumina, San Diego, CA, USA) for 16~18 h at 58℃, according to the manufacturer's instructions.

    Article Title: Phytochemical analysis and antioxidant and anticancer activities of mastic gum resin from Pistacia atlantica subspecies kurdica
    Article Snippet: qRT-PCR assay RNeasy® lipid tissue mini kit (Qiagen NV, Venlo, the Netherlands) was used to extract total RNA from COLO205 cells prior to conducting qRT-PCR analysis. .. The expression of Bax, Bcl-2, and Cyt-c genes was determined by qRT-PCR assay using GAPDH and β-actin genes as references.

    Real-time Polymerase Chain Reaction:

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations
    Article Snippet: Paragraph title: Quantitative Real-Time PCR ... Total RNA from adipose tissue was isolated using an RNeasy Lipid Tissue Mini kit (Qiagen, Hilden, Germany), following manufacturer's instructions. cDNA was synthesized from 800 ng total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA).

    Article Title: Initiation and progression of axonopathy in experimental autoimmune encephalomyelitis
    Article Snippet: RNA was isolated using RNeasy Lipid Tissue Mini Kit (QIAGEN, CA) and stored at −80°C. cDNA was prepared using Reaction Ready First Strand cDNA Synthesis kits (SuperArray Bioscience Corp., MD). .. Real-time PCR was performed using Mouse Toll Like Receptor Signaling Pathway Microarrays and Real-Time SYBR Green PCR Master Mixes (SuperArray Bioscience Corp., MD).

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. SCDH samples from individual mice were prepared for real-time quantitative PCR with Power SYBR Green master kit (PE Applied Biosystems, Foster City, CA) as directed.

    Article Title: Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats
    Article Snippet: Following removal of the RVLM-enriched tissue punches from −80°C storage, RNA from the tissue samples was extracted and purified using RNeasy Lipid Tissue mini kit (Qiagen) with QIAzol Lysis reagent (Qiagen) according to the manufacturer’s protocol. .. The purity of the RNA samples used for qPCR was estimated using Agilent High Sensitivity RNA ScreenTape System on an Agilent TapeStation (Agilent Technologies) and RNA samples with ≥7 RIN were used for qPCR.

    Hybridization:

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. RNA samples were processed for hybridization to the microarray chip and data were acquired according to Illumina BeadStation 500X manuals.

    Sequencing:

    Article Title: Characterization of Neural Estrogen Signaling and Neurotrophic Changes in the Accelerated Ovarian Failure Mouse Model of Menopause
    Article Snippet: Total RNA isolation from the dorsal hippocampus was performed using RNeasy Lipid Tissue Mini kit and QIAcube (QIAGEN) in accordance with the manufacturer's instructions. .. The qRT-PCR was performed using an Applied Biosystems 7900HT Sequence Detection System v2.3 using the SYBR Green PCR Master Mix.

    Cell Culture:

    Article Title: Egr-1 Regulates Expression of the Glial Scar Component Phosphacan in Astrocytes after Experimental Stroke
    Article Snippet: .. Total RNA was extracted and prepared from cultured cells as well as brain hemispheres (ipsilateral and contralateral) 10 days after permanent occlusion of the MCA using the RNA lipid tissue mini kit (Qiagen, Hilden, Germany). .. Residual genomic DNA was removed by the RNase-Free DNase Set (Qiagen) and RNA was reverse-transcribed into cDNA as follows: 1 μg of RNA was mixed with 100 ng of oligo(dT)15 and incubated for 5 minutes at 65°C.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Egr-1 Regulates Expression of the Glial Scar Component Phosphacan in Astrocytes after Experimental Stroke
    Article Snippet: Paragraph title: Isolation of Total RNA and Reverse Transcriptase (RT)-PCR Analysis ... Total RNA was extracted and prepared from cultured cells as well as brain hemispheres (ipsilateral and contralateral) 10 days after permanent occlusion of the MCA using the RNA lipid tissue mini kit (Qiagen, Hilden, Germany).

    Article Title: Mutant Huntingtin in Glial Cells Exacerbates Neurological Symptoms of Huntington Disease Mice *
    Article Snippet: Paragraph title: RT-PCR ... Total RNA from mouse cortex was isolated using the RNeasy Lipid Tissue Mini kit (Qiagen 74804).

    Isolation:

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations
    Article Snippet: .. Total RNA from adipose tissue was isolated using an RNeasy Lipid Tissue Mini kit (Qiagen, Hilden, Germany), following manufacturer's instructions. cDNA was synthesized from 800 ng total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA). .. Quantitative real-time PCR was performed using the following primer pairs: Vegfc , 5′-GAGGTCAAGGCTTTTGAAGGC-3′ (forward) and 5′-CTGTCCTGGTATTGAGGGTGG-3′ (reverse); adiponectin, 5′-GGAGATGCAGGTCTTCTT-3′ (forward) and 5′-CGAATGGGTACATTGGGAAC-3′ (reverse); and 36b4 , 5′-GGACCCGAGAAGACCTCCTT-3′ (forward) and 5′-GCACATCACTCAGAATTTC-3′ (reverse).

    Article Title: Initiation and progression of axonopathy in experimental autoimmune encephalomyelitis
    Article Snippet: .. RNA was isolated using RNeasy Lipid Tissue Mini Kit (QIAGEN, CA) and stored at −80°C. cDNA was prepared using Reaction Ready First Strand cDNA Synthesis kits (SuperArray Bioscience Corp., MD). .. Real-time PCR was performed using Mouse Toll Like Receptor Signaling Pathway Microarrays and Real-Time SYBR Green PCR Master Mixes (SuperArray Bioscience Corp., MD).

    Article Title: GENE EXPRESSION PROFILE OF MOUSE WHITE ADIPOSE TISSUE DURING INFLAMMATORY STRESS: AGE-DEPENDENT UPREGULATION OF MAJOR PRO-COAGULANT FACTORS
    Article Snippet: .. All centrifugation steps were performed at 4°C and 450 × g. Total RNA was isolated using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valenica, CA, USA). .. Reverse transcription was performed on 1μg of total RNA from adipose tissue, liver, and kidney and 500ng of RNA from isolated adipocytes and SVF cells, after repurification with RNAqueous (Ambion, Austin, TX, USA), using random primers and TaqMan® reverse transcription reagents (Applied Biosystems, Foster City, CA, USA).

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: .. Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The concentration and purity of RNA were assessed with an Eppendorf Biophotometer 6.31 spectrophotometer (Eppendorf, Westbury, NY).

    Article Title: Egr-1 Regulates Expression of the Glial Scar Component Phosphacan in Astrocytes after Experimental Stroke
    Article Snippet: Paragraph title: Isolation of Total RNA and Reverse Transcriptase (RT)-PCR Analysis ... Total RNA was extracted and prepared from cultured cells as well as brain hemispheres (ipsilateral and contralateral) 10 days after permanent occlusion of the MCA using the RNA lipid tissue mini kit (Qiagen, Hilden, Germany).

    Article Title: Mutant Huntingtin in Glial Cells Exacerbates Neurological Symptoms of Huntington Disease Mice *
    Article Snippet: .. Total RNA from mouse cortex was isolated using the RNeasy Lipid Tissue Mini kit (Qiagen 74804). .. First-strand cDNA was obtained using the Invitrogen SuperScriptTM First Strand Synthesis System for RT-PCR (11904-018), using a combination of random hexamer and Oligo(dT)12–18 primers.

    Article Title: Characterization of Neural Estrogen Signaling and Neurotrophic Changes in the Accelerated Ovarian Failure Mouse Model of Menopause
    Article Snippet: .. Total RNA isolation from the dorsal hippocampus was performed using RNeasy Lipid Tissue Mini kit and QIAcube (QIAGEN) in accordance with the manufacturer's instructions. .. Total RNA (0.5 μg) was reverse transcribed at 25°C for 10 minutes, 37°C for 120 minutes, and 85°C for 5 minutes using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems).

    Mouse Assay:

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations
    Article Snippet: Expression of adiponectin and Vegfc in organs of control mice and ADN–VEGF-C mice after 0.075 mg/mL doxycycline for 7 days was measured by quantitative real-time PCR. .. Total RNA from adipose tissue was isolated using an RNeasy Lipid Tissue Mini kit (Qiagen, Hilden, Germany), following manufacturer's instructions. cDNA was synthesized from 800 ng total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA).

    Article Title: Initiation and progression of axonopathy in experimental autoimmune encephalomyelitis
    Article Snippet: Pooled spinal cords from 3 MOG peptide, CFA control, or normal control mice were homogenized and stored in RNA later solution (Ambion, TX). .. RNA was isolated using RNeasy Lipid Tissue Mini Kit (QIAGEN, CA) and stored at −80°C. cDNA was prepared using Reaction Ready First Strand cDNA Synthesis kits (SuperArray Bioscience Corp., MD).

    Article Title: GENE EXPRESSION PROFILE OF MOUSE WHITE ADIPOSE TISSUE DURING INFLAMMATORY STRESS: AGE-DEPENDENT UPREGULATION OF MAJOR PRO-COAGULANT FACTORS
    Article Snippet: Perfused epididymal adipose tissues harvested from LPS-injected mice were cut into 30 mg (approximate size) pieces and digested with 1 mg/mL collagenase (Type I, Sigma-Aldrich, St. Louis, MO, USA) dissolved in 2% BSA-HBSS + 5mM glucose for 1h with agitation at 37°C. .. All centrifugation steps were performed at 4°C and 450 × g. Total RNA was isolated using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valenica, CA, USA).

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: .. Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The concentration and purity of RNA were assessed with an Eppendorf Biophotometer 6.31 spectrophotometer (Eppendorf, Westbury, NY).

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: .. Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. RNA samples were processed for hybridization to the microarray chip and data were acquired according to Illumina BeadStation 500X manuals.

    Polymerase Chain Reaction:

    Article Title: Initiation and progression of axonopathy in experimental autoimmune encephalomyelitis
    Article Snippet: RNA was isolated using RNeasy Lipid Tissue Mini Kit (QIAGEN, CA) and stored at −80°C. cDNA was prepared using Reaction Ready First Strand cDNA Synthesis kits (SuperArray Bioscience Corp., MD). .. Real-time PCR was performed using Mouse Toll Like Receptor Signaling Pathway Microarrays and Real-Time SYBR Green PCR Master Mixes (SuperArray Bioscience Corp., MD).

    Article Title: Egr-1 Regulates Expression of the Glial Scar Component Phosphacan in Astrocytes after Experimental Stroke
    Article Snippet: Total RNA was extracted and prepared from cultured cells as well as brain hemispheres (ipsilateral and contralateral) 10 days after permanent occlusion of the MCA using the RNA lipid tissue mini kit (Qiagen, Hilden, Germany). .. PCR was performed with 20 ng of cDNA using TaqDNA polymerase (Promega) for 24 to 35 cycles (1 minute at 95°C, 1 minute at 60 to 65°C, and 1 minute at 72°C).

    Article Title: Characterization of Neural Estrogen Signaling and Neurotrophic Changes in the Accelerated Ovarian Failure Mouse Model of Menopause
    Article Snippet: Total RNA isolation from the dorsal hippocampus was performed using RNeasy Lipid Tissue Mini kit and QIAcube (QIAGEN) in accordance with the manufacturer's instructions. .. The qRT-PCR was performed using an Applied Biosystems 7900HT Sequence Detection System v2.3 using the SYBR Green PCR Master Mix.

    Purification:

    Article Title: Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats
    Article Snippet: .. Following removal of the RVLM-enriched tissue punches from −80°C storage, RNA from the tissue samples was extracted and purified using RNeasy Lipid Tissue mini kit (Qiagen) with QIAzol Lysis reagent (Qiagen) according to the manufacturer’s protocol. .. The quality and quantity of the RNA used for microarray analysis were tested with the Agilent Bioanalyzer with RNA 6000 nano kit (Agilent Technologies).

    Article Title: Phytochemical analysis and antioxidant and anticancer activities of mastic gum resin from Pistacia atlantica subspecies kurdica
    Article Snippet: qRT-PCR assay RNeasy® lipid tissue mini kit (Qiagen NV, Venlo, the Netherlands) was used to extract total RNA from COLO205 cells prior to conducting qRT-PCR analysis. .. Highly purified salt-free primers were designed by Next Gene Scientific Sdn.

    Chromatin Immunoprecipitation:

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. RNA samples were processed for hybridization to the microarray chip and data were acquired according to Illumina BeadStation 500X manuals.

    Software:

    Article Title: Microarray Analysis of Differentially Expressed Genes in the Brains of Tubby Mice
    Article Snippet: Total RNA was prepared from the cerebral cortex and hypothalamus of tubby and control mice using the RNeasy lipid tissue mini-kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. Hybridization signals on the chip were analyzed by an Illumina bead array confocal scanner and Illumina Beadstudio v3.1.3 software (Gene Expression Module v3.3.8).

    Electrophoresis:

    Article Title: Gene Expression in the Spinal Cord in Female Lewis Rats with Experimental Autoimmune Encephalomyelitis Induced with Myelin Basic Protein
    Article Snippet: RNA quality analysis was carried out on the BioRad Experion automated electrophoresis system. .. Tissue samples were snap frozen in liquid nitrogen and stored at −80°C prior to total RNA preparation using the QIAGEN RNeasy Lipid tissue kit.

    Incubation:

    Article Title: Egr-1 Regulates Expression of the Glial Scar Component Phosphacan in Astrocytes after Experimental Stroke
    Article Snippet: Total RNA was extracted and prepared from cultured cells as well as brain hemispheres (ipsilateral and contralateral) 10 days after permanent occlusion of the MCA using the RNA lipid tissue mini kit (Qiagen, Hilden, Germany). .. Residual genomic DNA was removed by the RNase-Free DNase Set (Qiagen) and RNA was reverse-transcribed into cDNA as follows: 1 μg of RNA was mixed with 100 ng of oligo(dT)15 and incubated for 5 minutes at 65°C.

    Spectrophotometry:

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The concentration and purity of RNA were assessed with an Eppendorf Biophotometer 6.31 spectrophotometer (Eppendorf, Westbury, NY).

    Concentration Assay:

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Total RNA was extracted from SCDH of individual mice using Qiagen RNeasy Lipid Tissue Mini Kit and isolated using RNase-free DNase kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The concentration and purity of RNA were assessed with an Eppendorf Biophotometer 6.31 spectrophotometer (Eppendorf, Westbury, NY).

    Lysis:

    Article Title: Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats
    Article Snippet: .. Following removal of the RVLM-enriched tissue punches from −80°C storage, RNA from the tissue samples was extracted and purified using RNeasy Lipid Tissue mini kit (Qiagen) with QIAzol Lysis reagent (Qiagen) according to the manufacturer’s protocol. .. The quality and quantity of the RNA used for microarray analysis were tested with the Agilent Bioanalyzer with RNA 6000 nano kit (Agilent Technologies).

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  • 99
    Qiagen rneasy lipid tissue minikit
    Rneasy Lipid Tissue Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy lipid tissue minikit/product/Qiagen
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    rneasy lipid tissue minikit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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