rneasy kits  (Qiagen)


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    Name:
    RNeasy Plus Mini Kit
    Description:
    For phenol free total RNA extraction from cells tissues with removal of genomic DNA contamination Kit contents Qiagen RNeasy Plus Mini Kit 50 preps 30mg Sample 30 to 50L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format 25 min Time Run Ideal for PCR Northern Dot and Slot Blotting Array Analysis Sequencing For Purification of Up to 100g Total RNA from Cells tissues Using gDNA Eliminator Columns Includes RNeasy Mini Spin Columns gDNA Eliminator Spin Columns Collection Tubes RNase free Water and Buffers Benefits Efficient gDNA removal with unique gDNA Eliminator columns no need for DNase High quality total RNA in minutes using fast and simple extraction protocols Phenol free RNA isolation Ideal for sensitive applications such as real time RT PCR and RNA Seq
    Catalog Number:
    74134
    Price:
    360
    Category:
    RNeasy Plus Micro and Mini Kits
    Buy from Supplier


    Structured Review

    Qiagen rneasy kits
    RNeasy Plus Mini Kit
    For phenol free total RNA extraction from cells tissues with removal of genomic DNA contamination Kit contents Qiagen RNeasy Plus Mini Kit 50 preps 30mg Sample 30 to 50L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format 25 min Time Run Ideal for PCR Northern Dot and Slot Blotting Array Analysis Sequencing For Purification of Up to 100g Total RNA from Cells tissues Using gDNA Eliminator Columns Includes RNeasy Mini Spin Columns gDNA Eliminator Spin Columns Collection Tubes RNase free Water and Buffers Benefits Efficient gDNA removal with unique gDNA Eliminator columns no need for DNase High quality total RNA in minutes using fast and simple extraction protocols Phenol free RNA isolation Ideal for sensitive applications such as real time RT PCR and RNA Seq
    https://www.bioz.com/result/rneasy kits/product/Qiagen
    Average 90 stars, based on 447 article reviews
    Price from $9.99 to $1999.99
    rneasy kits - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Long non-coding RNA MUC5B-AS1 promotes metastasis through mutually regulating MUC5B expression in lung adenocarcinoma"

    Article Title: Long non-coding RNA MUC5B-AS1 promotes metastasis through mutually regulating MUC5B expression in lung adenocarcinoma

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0472-6

    MUC5B-AS1 increases the stability of MUC5B mRNA by forming a protective RNA duplex. a Schematic representation of the PCR amplification regions for overlapping (OL) and non-overlapping (non-OL) regions of MUC5B. We designed two pairs of primers to amplify the OL regions (OL1 and OL2) and non-OL (non-OL1 and non-OL2) regions of MUC5B, respectively. F forward primer, R reverse primer. b RT-PCR products of OL and non-OL regions of MUC5B. Total RNA samples were treated with RNAse A + T cocktail and then cleaned up RNA using RNeasy kits. RT-PCR was conducted using the primers to detect the OL and non-OL regions of the MUC5B mRNA. OL and non-OL regions of KRT7-AS were used as a positive control. c Stability of MUC5B mRNA over 12 h was measured by qRT-PCR relative to time 0 h after blocking new RNA synthesis with Actinomycin D (1 μg/mL; indicated with black arrow). H1299 cells with MUC5B-AS1 or empty vector stable expression were treated with 1 μg/mL ActD, and then harvested cells for RNA purification at 12 h after addition of ActD. Then, MUC5B mRNA stability were subsequently measured by qRT-PCR and were normalized against a synthesized exogenous reference λ polyA + RNA. Student’s t -test, * P
    Figure Legend Snippet: MUC5B-AS1 increases the stability of MUC5B mRNA by forming a protective RNA duplex. a Schematic representation of the PCR amplification regions for overlapping (OL) and non-overlapping (non-OL) regions of MUC5B. We designed two pairs of primers to amplify the OL regions (OL1 and OL2) and non-OL (non-OL1 and non-OL2) regions of MUC5B, respectively. F forward primer, R reverse primer. b RT-PCR products of OL and non-OL regions of MUC5B. Total RNA samples were treated with RNAse A + T cocktail and then cleaned up RNA using RNeasy kits. RT-PCR was conducted using the primers to detect the OL and non-OL regions of the MUC5B mRNA. OL and non-OL regions of KRT7-AS were used as a positive control. c Stability of MUC5B mRNA over 12 h was measured by qRT-PCR relative to time 0 h after blocking new RNA synthesis with Actinomycin D (1 μg/mL; indicated with black arrow). H1299 cells with MUC5B-AS1 or empty vector stable expression were treated with 1 μg/mL ActD, and then harvested cells for RNA purification at 12 h after addition of ActD. Then, MUC5B mRNA stability were subsequently measured by qRT-PCR and were normalized against a synthesized exogenous reference λ polyA + RNA. Student’s t -test, * P

    Techniques Used: Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction, Positive Control, Quantitative RT-PCR, Blocking Assay, Plasmid Preparation, Expressing, Purification, Synthesized

    Related Articles

    Clone Assay:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. .. Complementary RNA libraries were prepared following the previously published protocol (Heyer et al., ) and quality control of the DNA colony template library was performed by cloning an aliquot into a TOPO plasmid and sequencing 8/10 clones.

    Synthesized:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed. .. The PHT1 and PHT2 primers and probes were designed with Primer Express 3.0 software (Applied Biosystems, Foster City, CA), and the primers, probes and standard DNA were synthesized by Integrated DNA Technologies (Coralville, IA).

    Article Title: A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors
    Article Snippet: .. Brains were removed, and the striatum was dissected on ice using a 1mm coronal matrix, placed in RNAlater, homogenized, and total RNA was purified using RNeasy Plus Mini kit (QIAGEN). cDNA was synthesized using iScript Reverse Transcription Supermix (Biorad). .. Actb (Mm01205647) and Drd1 (Mm02620146_s1) TaqMan Gene Expression Assays (Applied Biosystems) were used to determine relative mRNA expression of the endogenous control gene β-actin and DA D1Rs, respectively.

    Article Title: Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways, et al. Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways
    Article Snippet: Then, DNase‐treated RNA was isolated from cells by using an RNeasy Plus Mini Kit (Qiagen). .. DNase‐treated RNA was isolated by using an RNeasy Plus Mini Kit. cDNA was synthesized by using SuperScript III First‐Strand Synthesis SuperMix for quantitative RT‐PCR (Thermo Fisher Scientific).

    Article Title: Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1 , 2
    Article Snippet: .. Afterward, total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations. cDNA were synthesized by iScript reverse transcription (Bio-Rad, Hercules, CA), which were then diluted 10-fold in H2 O and used in semiquantitative real-time PCR reactions (25 µl) that used the SYBR Green system (Bio-Rad) supplemented with 5 µl of diluted cDNA and 0.1 µM of oligonucleotide pairs listed below. .. Polymerase chain reactions were performed and analyzed on a Bio-Rad Mini-Opticon detection system, and differences in RNA concentrations were controlled by normalizing individual gene signals to their corresponding β-actin or GAPDH RNA signals.

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers. .. Validated primers were purchased from Qiagen or synthesized by Integrated DNA Technologies (Coralville, IA) (see ). qPCR reactions were performed using a 7300 Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA): Hot-Start activation at 95°C for 10 min, 40 cycles of denaturation (95°C for 15 sec) and annealing/extension (55°C or 60°C for 60 sec).

    Article Title: Bidirectional histone-gene promoters in Aspergillus: characterization and application for multi-gene expression
    Article Snippet: RNA extraction, cDNA synthesis, RT-qPCR and data analysis RNA was extracted, using the RNeasy Plus Mini Kit (Qiagen), from biomass harvested from 3-day cultivations on solid medium and 6, 12, 24, 48 and 72 h submerged cultivations. .. The cDNA was synthesized using the SensiFAST cDNA Synthesis Kit (Bioline) according to manufacturer’s recommendations.

    Co-Culture Assay:

    Article Title: Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
    Article Snippet: .. RNA extraction and quantitative real-time polymerase chain reaction (qPCR) To analyse the expression of proinflammatory cytokines, total RNA was extracted from J774A.1 cells after 24 hours of co-culture with bacteria, using an RNeasy Plus Mini kit (Qiagen, Tokyo, Japan). .. Total RNA of A. baumannii cultured in LB broth for 6 hours at 37 °C was extracted using an RNeasy Protect Bacteria mini kit (Qiagen).

    SYBR Green Assay:

    Article Title: Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma
    Article Snippet: .. Reverse-transcriptase quantitative PCR RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step reverse-transcriptase quantitative PCR (RT-qPCR) Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. .. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M ) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) were used as internal controls. mRNA-specific primers were designed to span exon-exon junctions to avoid detection of genomic DNA.

    Article Title: Dopamine D2 receptor modulates Wnt expression and control of cell proliferation
    Article Snippet: Quantitative Real-Time PCR Total RNA was purified using the RNeasy Plus Mini kit (Qiagen, Valencia, CA). cDNA was prepared using an RT2 First Strand kit as per the manufacturer’s protocol (SABiosciences-Qiagen). .. The assay used SYBR Green Real-Time PCR detection method (Qiagen) and all gene-specific primer assays were commercially available in the RT2 qPCR Primer Assay format, (SABiosciences-Qiagen) following manufacturer’s instructions.

    Article Title: Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling
    Article Snippet: Quantitative RT-PCR Total RNA was extracted with the RNeasy Plus Mini Kit from Qiagen (Hilden, Germany) according to the manufacturer’s instructions. .. The cDNAs were then mixed with Qi SYBR Green Supermix (Bio-Rad) for quantitative RT-PCR assay using a Bio-Rad I-cycler IQ quantitative thermocycler.

    Article Title: Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1 , 2
    Article Snippet: .. Afterward, total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations. cDNA were synthesized by iScript reverse transcription (Bio-Rad, Hercules, CA), which were then diluted 10-fold in H2 O and used in semiquantitative real-time PCR reactions (25 µl) that used the SYBR Green system (Bio-Rad) supplemented with 5 µl of diluted cDNA and 0.1 µM of oligonucleotide pairs listed below. .. Polymerase chain reactions were performed and analyzed on a Bio-Rad Mini-Opticon detection system, and differences in RNA concentrations were controlled by normalizing individual gene signals to their corresponding β-actin or GAPDH RNA signals.

    Article Title: Synchronous inhibition of mTOR and VEGF/NRP1 axis impedes tumor growth and metastasis in renal cancer
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    Article Title: CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming
    Article Snippet: Cells were harvested using Accutase (STEMCELL), and total RNA was isolated using the RNeasy Plus Mini Kit (QIAGEN), according to manufacturer’s instructions. .. Quantitative PCR reactions were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad).

    Article Title: Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries
    Article Snippet: Quantitative RT-PCR RNA was extracted from cells using RNeasy® Plus Mini Kit (Qiagen Inc., Valencia, CA) according to manufacturer’s instructions. .. Quantitative, RT-PCR was carried out on 384 well plates using POWER SYBR Green (Applied Biosystems, Foster City, CA) according to the manufacturer’s instructions on an Applied Biosystems 7900 HT instrument.

    Quantitation Assay:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: Quantitation of PHT1 and PHT2 transcripts was performed on the small intestine, colon and kidney from wild-type and Pept1 null mice using the 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA). .. Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed.

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: The absorbance was normalized by dividing by the absorbance measured in wells that received osteogenic medium but were not treated and reported as “Fold Difference.” Following the pNPP assay, cells were stained with Alizarin Red (Osteogenesis Quantitation Kit, Millipore, Billerica, MA), washed and then photographed using the UVP Bioimaging System (UVP, Inc., Upland, CA). .. Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers.

    Expressing:

    Article Title: Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma
    Article Snippet: .. Reverse-transcriptase quantitative PCR RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step reverse-transcriptase quantitative PCR (RT-qPCR) Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. .. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M ) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) were used as internal controls. mRNA-specific primers were designed to span exon-exon junctions to avoid detection of genomic DNA.

    Article Title: Dopamine D2 receptor modulates Wnt expression and control of cell proliferation
    Article Snippet: Quantitative Real-Time PCR Total RNA was purified using the RNeasy Plus Mini kit (Qiagen, Valencia, CA). cDNA was prepared using an RT2 First Strand kit as per the manufacturer’s protocol (SABiosciences-Qiagen). .. Quantitative gene expression was analyzed by real-time PCR, performed on an ABI Prism 7900 HT (Thermo Fisher, Pittsburgh, PA).

    Article Title: A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors
    Article Snippet: Brains were removed, and the striatum was dissected on ice using a 1mm coronal matrix, placed in RNAlater, homogenized, and total RNA was purified using RNeasy Plus Mini kit (QIAGEN). cDNA was synthesized using iScript Reverse Transcription Supermix (Biorad). .. Actb (Mm01205647) and Drd1 (Mm02620146_s1) TaqMan Gene Expression Assays (Applied Biosystems) were used to determine relative mRNA expression of the endogenous control gene β-actin and DA D1Rs, respectively.

    Article Title: Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways, et al. Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways
    Article Snippet: 2.7 Quantitative RT‐PCR TaqMan Gene Expression Assays (gene symbols and assay IDs are shown in Table ) were purchased from Thermo Fisher Scientific. .. Then, DNase‐treated RNA was isolated from cells by using an RNeasy Plus Mini Kit (Qiagen).

    Article Title: Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1 , 2
    Article Snippet: The effect of CystC and Δ14CystC on the ability of TGF-β to regulate gene expression in either NMuMG, 4T1, or MB114 cells was assessed by semiquantitative real-time PCR as described previously [ ]. .. Afterward, total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations. cDNA were synthesized by iScript reverse transcription (Bio-Rad, Hercules, CA), which were then diluted 10-fold in H2 O and used in semiquantitative real-time PCR reactions (25 µl) that used the SYBR Green system (Bio-Rad) supplemented with 5 µl of diluted cDNA and 0.1 µM of oligonucleotide pairs listed below.

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers. .. Relative gene expression was determined using the Pfaffl method to account for differential primer efficiencies ( ).

    Article Title: Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
    Article Snippet: .. RNA extraction and quantitative real-time polymerase chain reaction (qPCR) To analyse the expression of proinflammatory cytokines, total RNA was extracted from J774A.1 cells after 24 hours of co-culture with bacteria, using an RNeasy Plus Mini kit (Qiagen, Tokyo, Japan). .. Total RNA of A. baumannii cultured in LB broth for 6 hours at 37 °C was extracted using an RNeasy Protect Bacteria mini kit (Qiagen).

    Concentration Assay:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: One mL of adjusted and washed bacterial suspension was centrifuged at 13,000 g, and the pellets were treated with lysostaphin (Sigma-Aldrich) at a final concentration of 200 mg/L. .. The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: After quenching with NaOH (final concentration: 0.75M), absorbance (405 nM) was measured using a Synergy2 multifunction plate reader (Biotek Inc., Winooski, WT). .. Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers.

    Cell Culture:

    Article Title: Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1 , 2
    Article Snippet: The following morning, the cells were washed in PBS and cultured in serum-free medium supplemented with or without TGF-β1 (5 ng/ml) for varying times at 37°C. .. Afterward, total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations. cDNA were synthesized by iScript reverse transcription (Bio-Rad, Hercules, CA), which were then diluted 10-fold in H2 O and used in semiquantitative real-time PCR reactions (25 µl) that used the SYBR Green system (Bio-Rad) supplemented with 5 µl of diluted cDNA and 0.1 µM of oligonucleotide pairs listed below.

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Paragraph title: 2.2. Primary osteoblast cell culture ... Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers.

    Article Title: Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qPCR) To analyse the expression of proinflammatory cytokines, total RNA was extracted from J774A.1 cells after 24 hours of co-culture with bacteria, using an RNeasy Plus Mini kit (Qiagen, Tokyo, Japan). .. Total RNA of A. baumannii cultured in LB broth for 6 hours at 37 °C was extracted using an RNeasy Protect Bacteria mini kit (Qiagen).

    Sequencing:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: Paragraph title: RNA Preparation and Whole Transcriptome Sequencing (RNA-seq) ... The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions.

    RNA Sequencing Assay:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: Paragraph title: RNA Preparation and Whole Transcriptome Sequencing (RNA-seq) ... The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions.

    Article Title: Investigating RNA expression profiles altered by nicotinamide mononucleotide therapy in a chronic model of alcoholic liver disease
    Article Snippet: .. RNA sequencing Total RNA was isolated and purified from mouse liver (n = 3 per group) according to the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). .. RNA quality and concentrations were determined by NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Isolation:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: .. Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed. .. The PHT1 and PHT2 primers and probes were designed with Primer Express 3.0 software (Applied Biosystems, Foster City, CA), and the primers, probes and standard DNA were synthesized by Integrated DNA Technologies (Coralville, IA).

    Article Title: Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma
    Article Snippet: .. Reverse-transcriptase quantitative PCR RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step reverse-transcriptase quantitative PCR (RT-qPCR) Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. .. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M ) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) were used as internal controls. mRNA-specific primers were designed to span exon-exon junctions to avoid detection of genomic DNA.

    Article Title: Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways, et al. Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways
    Article Snippet: .. Then, DNase‐treated RNA was isolated from cells by using an RNeasy Plus Mini Kit (Qiagen). .. For the extraction of RNA from tumors, the excised tumor was soaked in ISOGEN (Nippon Gene) and then homogenized with Pellet Mixer for Microtubes 1.5 mL (Nolato Treff).

    Article Title: Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1 , 2
    Article Snippet: .. Afterward, total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations. cDNA were synthesized by iScript reverse transcription (Bio-Rad, Hercules, CA), which were then diluted 10-fold in H2 O and used in semiquantitative real-time PCR reactions (25 µl) that used the SYBR Green system (Bio-Rad) supplemented with 5 µl of diluted cDNA and 0.1 µM of oligonucleotide pairs listed below. .. Polymerase chain reactions were performed and analyzed on a Bio-Rad Mini-Opticon detection system, and differences in RNA concentrations were controlled by normalizing individual gene signals to their corresponding β-actin or GAPDH RNA signals.

    Article Title: Synchronous inhibition of mTOR and VEGF/NRP1 axis impedes tumor growth and metastasis in renal cancer
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) Total RNA was isolated from a portion of the tumors using RNeasy Plus Mini Kit (Qiagen) as per the manufacturer’s instructions. .. Reverse transcription was performed using iScript™ cDNA Synthesis Kit (Bio-Rad).

    Article Title: CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming
    Article Snippet: .. Cells were harvested using Accutase (STEMCELL), and total RNA was isolated using the RNeasy Plus Mini Kit (QIAGEN), according to manufacturer’s instructions. .. Reverse transcription was performed using iScript cDNA Synthesis kit (Bio-Rad).

    Article Title: Investigating RNA expression profiles altered by nicotinamide mononucleotide therapy in a chronic model of alcoholic liver disease
    Article Snippet: .. RNA sequencing Total RNA was isolated and purified from mouse liver (n = 3 per group) according to the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). .. RNA quality and concentrations were determined by NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Size-exclusion Chromatography:

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers. .. Validated primers were purchased from Qiagen or synthesized by Integrated DNA Technologies (Coralville, IA) (see ). qPCR reactions were performed using a 7300 Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA): Hot-Start activation at 95°C for 10 min, 40 cycles of denaturation (95°C for 15 sec) and annealing/extension (55°C or 60°C for 60 sec).

    Purification:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: .. The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. .. Removal of rRNAs was performed using the Ribo Zero kit (Illumina), following the manufacturer's instructions, and each RNA sample was suspended in 30 μL of RNA storage solution.

    Article Title: Dopamine D2 receptor modulates Wnt expression and control of cell proliferation
    Article Snippet: .. Quantitative Real-Time PCR Total RNA was purified using the RNeasy Plus Mini kit (Qiagen, Valencia, CA). cDNA was prepared using an RT2 First Strand kit as per the manufacturer’s protocol (SABiosciences-Qiagen). .. Quantitative gene expression was analyzed by real-time PCR, performed on an ABI Prism 7900 HT (Thermo Fisher, Pittsburgh, PA).

    Article Title: A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors
    Article Snippet: .. Brains were removed, and the striatum was dissected on ice using a 1mm coronal matrix, placed in RNAlater, homogenized, and total RNA was purified using RNeasy Plus Mini kit (QIAGEN). cDNA was synthesized using iScript Reverse Transcription Supermix (Biorad). .. Actb (Mm01205647) and Drd1 (Mm02620146_s1) TaqMan Gene Expression Assays (Applied Biosystems) were used to determine relative mRNA expression of the endogenous control gene β-actin and DA D1Rs, respectively.

    Article Title: Investigating RNA expression profiles altered by nicotinamide mononucleotide therapy in a chronic model of alcoholic liver disease
    Article Snippet: .. RNA sequencing Total RNA was isolated and purified from mouse liver (n = 3 per group) according to the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). .. RNA quality and concentrations were determined by NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Synchronous inhibition of mTOR and VEGF/NRP1 axis impedes tumor growth and metastasis in renal cancer
    Article Snippet: Quantitative polymerase chain reaction (qPCR) Total RNA was isolated from a portion of the tumors using RNeasy Plus Mini Kit (Qiagen) as per the manufacturer’s instructions. .. Finally, qPCR was performed for the specified targets using Power SYBR Green PCR Master Mix (Applied Bioscience) in an ABI 7500 Real-Time PCR System (Applied Bioscience).

    Quantitative RT-PCR:

    Article Title: Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma
    Article Snippet: .. Reverse-transcriptase quantitative PCR RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step reverse-transcriptase quantitative PCR (RT-qPCR) Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. .. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M ) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) were used as internal controls. mRNA-specific primers were designed to span exon-exon junctions to avoid detection of genomic DNA.

    Article Title: Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways, et al. Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways
    Article Snippet: Paragraph title: Quantitative RT‐PCR ... Then, DNase‐treated RNA was isolated from cells by using an RNeasy Plus Mini Kit (Qiagen).

    Article Title: Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling
    Article Snippet: .. Quantitative RT-PCR Total RNA was extracted with the RNeasy Plus Mini Kit from Qiagen (Hilden, Germany) according to the manufacturer’s instructions. .. One microgram of RNA was reverse-transcribed using an iScript Reverse Transcription Supermix from Bio-Rad Laboratories (Hercules, CA, USA) to make single-stranded cDNA.

    Article Title: CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming
    Article Snippet: Paragraph title: Quantitative RT-PCR ... Cells were harvested using Accutase (STEMCELL), and total RNA was isolated using the RNeasy Plus Mini Kit (QIAGEN), according to manufacturer’s instructions.

    Article Title: Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries
    Article Snippet: .. Quantitative RT-PCR RNA was extracted from cells using RNeasy® Plus Mini Kit (Qiagen Inc., Valencia, CA) according to manufacturer’s instructions. ..

    Article Title: Bidirectional histone-gene promoters in Aspergillus: characterization and application for multi-gene expression
    Article Snippet: .. RNA extraction, cDNA synthesis, RT-qPCR and data analysis RNA was extracted, using the RNeasy Plus Mini Kit (Qiagen), from biomass harvested from 3-day cultivations on solid medium and 6, 12, 24, 48 and 72 h submerged cultivations. .. The cDNA was synthesized using the SensiFAST cDNA Synthesis Kit (Bioline) according to manufacturer’s recommendations.

    IA:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed. .. The PHT1 and PHT2 primers and probes were designed with Primer Express 3.0 software (Applied Biosystems, Foster City, CA), and the primers, probes and standard DNA were synthesized by Integrated DNA Technologies (Coralville, IA).

    Mouse Assay:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: Quantitation of PHT1 and PHT2 transcripts was performed on the small intestine, colon and kidney from wild-type and Pept1 null mice using the 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA). .. Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed.

    Article Title: A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors
    Article Snippet: Quantitative Polymerase Chain Reaction Mice were anesthetized with pentobarbital and decapitated. .. Brains were removed, and the striatum was dissected on ice using a 1mm coronal matrix, placed in RNAlater, homogenized, and total RNA was purified using RNeasy Plus Mini kit (QIAGEN). cDNA was synthesized using iScript Reverse Transcription Supermix (Biorad).

    Plasmid Preparation:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. .. Complementary RNA libraries were prepared following the previously published protocol (Heyer et al., ) and quality control of the DNA colony template library was performed by cloning an aliquot into a TOPO plasmid and sequencing 8/10 clones.

    Software:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed. .. The PHT1 and PHT2 primers and probes were designed with Primer Express 3.0 software (Applied Biosystems, Foster City, CA), and the primers, probes and standard DNA were synthesized by Integrated DNA Technologies (Coralville, IA).

    Article Title: Bidirectional histone-gene promoters in Aspergillus: characterization and application for multi-gene expression
    Article Snippet: RNA extraction, cDNA synthesis, RT-qPCR and data analysis RNA was extracted, using the RNeasy Plus Mini Kit (Qiagen), from biomass harvested from 3-day cultivations on solid medium and 6, 12, 24, 48 and 72 h submerged cultivations. .. RT-qPCR conducted using the SensiFAST SYBR No-ROX Kit (Bioline) according to manufacturer’s recommendations, on a CFX Connect (BioRad) using the software BioRad CFX Manager 3.1, and white 96 well RT-qPCR plates and clear lids (VWR International).

    Real-time Polymerase Chain Reaction:

    Article Title: Targeted Disruption of Peptide Transporter Pept1 Gene in Mice Significantly Reduces Dipeptide Absorption in Intestine
    Article Snippet: Paragraph title: Taqman Real-Time PCR Analyses ... Total RNA was isolated according to the manufacturer's protocol using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA), and then reverse-transcribed.

    Article Title: Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma
    Article Snippet: .. Reverse-transcriptase quantitative PCR RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). mRNA expression was determined using SYBR green Luna One Step reverse-transcriptase quantitative PCR (RT-qPCR) Kit (New England BioLabs) on a C1000 Touch (Bio-Rad) or QuantStudio 6 (Applied Biosystems) thermocycler. .. Expression was quantified by the ΔΔCt method using target-specific and control primers. β-2-microglobulin (B2M ) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) were used as internal controls. mRNA-specific primers were designed to span exon-exon junctions to avoid detection of genomic DNA.

    Article Title: Dopamine D2 receptor modulates Wnt expression and control of cell proliferation
    Article Snippet: .. Quantitative Real-Time PCR Total RNA was purified using the RNeasy Plus Mini kit (Qiagen, Valencia, CA). cDNA was prepared using an RT2 First Strand kit as per the manufacturer’s protocol (SABiosciences-Qiagen). .. Quantitative gene expression was analyzed by real-time PCR, performed on an ABI Prism 7900 HT (Thermo Fisher, Pittsburgh, PA).

    Article Title: A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors
    Article Snippet: Paragraph title: Quantitative Polymerase Chain Reaction ... Brains were removed, and the striatum was dissected on ice using a 1mm coronal matrix, placed in RNAlater, homogenized, and total RNA was purified using RNeasy Plus Mini kit (QIAGEN). cDNA was synthesized using iScript Reverse Transcription Supermix (Biorad).

    Article Title: Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways, et al. Activity of TAS4464, a novel NEDD8 activating enzyme E1 inhibitor, against multiple myeloma via inactivation of nuclear factor κB pathways
    Article Snippet: Then, DNase‐treated RNA was isolated from cells by using an RNeasy Plus Mini Kit (Qiagen). .. Quantitative PCR (qPCR) was performed by using a 7900HT Fast Real‐Time PCR System (Applied Biosystems) or a QuantStudio 7 Flex System (Thermo Fisher Scientific).

    Article Title: Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1Preclinical Efficacy of Cystatin C to Target the Oncogenic Activity of Transforming Growth Factor ? in Breast Cancer 1 , 2
    Article Snippet: .. Afterward, total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations. cDNA were synthesized by iScript reverse transcription (Bio-Rad, Hercules, CA), which were then diluted 10-fold in H2 O and used in semiquantitative real-time PCR reactions (25 µl) that used the SYBR Green system (Bio-Rad) supplemented with 5 µl of diluted cDNA and 0.1 µM of oligonucleotide pairs listed below. .. Polymerase chain reactions were performed and analyzed on a Bio-Rad Mini-Opticon detection system, and differences in RNA concentrations were controlled by normalizing individual gene signals to their corresponding β-actin or GAPDH RNA signals.

    Article Title: Synchronous inhibition of mTOR and VEGF/NRP1 axis impedes tumor growth and metastasis in renal cancer
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) Total RNA was isolated from a portion of the tumors using RNeasy Plus Mini Kit (Qiagen) as per the manufacturer’s instructions. .. Reverse transcription was performed using iScript™ cDNA Synthesis Kit (Bio-Rad).

    Article Title: CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming
    Article Snippet: Cells were harvested using Accutase (STEMCELL), and total RNA was isolated using the RNeasy Plus Mini Kit (QIAGEN), according to manufacturer’s instructions. .. Quantitative PCR reactions were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad).

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers. .. All qPCR reactions were performed using the GoTaq® qPCR Master Mix System (Promega).

    Article Title: Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
    Article Snippet: .. RNA extraction and quantitative real-time polymerase chain reaction (qPCR) To analyse the expression of proinflammatory cytokines, total RNA was extracted from J774A.1 cells after 24 hours of co-culture with bacteria, using an RNeasy Plus Mini kit (Qiagen, Tokyo, Japan). .. Total RNA of A. baumannii cultured in LB broth for 6 hours at 37 °C was extracted using an RNeasy Protect Bacteria mini kit (Qiagen).

    RNA Extraction:

    Article Title: Bidirectional histone-gene promoters in Aspergillus: characterization and application for multi-gene expression
    Article Snippet: .. RNA extraction, cDNA synthesis, RT-qPCR and data analysis RNA was extracted, using the RNeasy Plus Mini Kit (Qiagen), from biomass harvested from 3-day cultivations on solid medium and 6, 12, 24, 48 and 72 h submerged cultivations. .. The cDNA was synthesized using the SensiFAST cDNA Synthesis Kit (Bioline) according to manufacturer’s recommendations.

    Article Title: Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
    Article Snippet: .. RNA extraction and quantitative real-time polymerase chain reaction (qPCR) To analyse the expression of proinflammatory cytokines, total RNA was extracted from J774A.1 cells after 24 hours of co-culture with bacteria, using an RNeasy Plus Mini kit (Qiagen, Tokyo, Japan). .. Total RNA of A. baumannii cultured in LB broth for 6 hours at 37 °C was extracted using an RNeasy Protect Bacteria mini kit (Qiagen).

    Incubation:

    Article Title: Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection
    Article Snippet: RNA Preparation and Whole Transcriptome Sequencing (RNA-seq) Overnight bacterial cultures were used to inoculate a BHI broth and incubated at 36°C with gyratory shaking at 200 rpm. .. The total RNA of the pellets was then purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions.

    Spectrophotometry:

    Article Title: Investigating RNA expression profiles altered by nicotinamide mononucleotide therapy in a chronic model of alcoholic liver disease
    Article Snippet: RNA sequencing Total RNA was isolated and purified from mouse liver (n = 3 per group) according to the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). .. RNA quality and concentrations were determined by NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qPCR) To analyse the expression of proinflammatory cytokines, total RNA was extracted from J774A.1 cells after 24 hours of co-culture with bacteria, using an RNeasy Plus Mini kit (Qiagen, Tokyo, Japan). .. Harvested RNA samples were quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific, MA, USA).

    Activation Assay:

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers. .. Validated primers were purchased from Qiagen or synthesized by Integrated DNA Technologies (Coralville, IA) (see ). qPCR reactions were performed using a 7300 Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA): Hot-Start activation at 95°C for 10 min, 40 cycles of denaturation (95°C for 15 sec) and annealing/extension (55°C or 60°C for 60 sec).

    Construct:

    Article Title: Investigating RNA expression profiles altered by nicotinamide mononucleotide therapy in a chronic model of alcoholic liver disease
    Article Snippet: RNA sequencing Total RNA was isolated and purified from mouse liver (n = 3 per group) according to the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). .. RNA libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit (San Diego, CA, USA) in accordance with the manufacturer’s protocol.

    Staining:

    Article Title: Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice
    Article Snippet: Following image capture, Alizarin Red staining was quantified as indicated in the manufacturer’s instructions. .. Total RNA was extracted and genomic DNA was removed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers.

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    Qiagen rneasy plus mini kit
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy micro kit
    Rneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy micro kit/product/Qiagen
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    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 8038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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