rneasy fibrous tissue minikit  (Qiagen)


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    Name:
    RNeasy Fibrous Tissue Mini Kit
    Description:
    For purification of up to 100 µg total RNA from fiber rich tissues Kit contents Qiagen RNeasy Fibrous Tissue Mini Kit 50 preps 0 5 to 30mg Sample 30 to 100L Elution Volume Fibre rich Tissue Sample RNA Purification Spin Column Format Silica Technology 55 min Time Run For PCR Real time PCR Microarray Includes 50 RNeasy Mini Spin Columns Collection Tubes 1 5 and 2mL Proteinase K RNase free DNase I RNase free Reagents and Buffers Benefits Optimized protocols for fibrous tissues High yields of total RNA High quality RNA for all downstream applications Removal of genomic DNA using DNase I
    Catalog Number:
    74704
    Price:
    388
    Category:
    RNeasy Fibrous Tissue Mini Kit
    Buy from Supplier


    Structured Review

    Qiagen rneasy fibrous tissue minikit
    RNeasy Fibrous Tissue Mini Kit
    For purification of up to 100 µg total RNA from fiber rich tissues Kit contents Qiagen RNeasy Fibrous Tissue Mini Kit 50 preps 0 5 to 30mg Sample 30 to 100L Elution Volume Fibre rich Tissue Sample RNA Purification Spin Column Format Silica Technology 55 min Time Run For PCR Real time PCR Microarray Includes 50 RNeasy Mini Spin Columns Collection Tubes 1 5 and 2mL Proteinase K RNase free DNase I RNase free Reagents and Buffers Benefits Optimized protocols for fibrous tissues High yields of total RNA High quality RNA for all downstream applications Removal of genomic DNA using DNase I
    https://www.bioz.com/result/rneasy fibrous tissue minikit/product/Qiagen
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    rneasy fibrous tissue minikit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Sclerostin as Regulatory Molecule in Vascular Media Calcification and the Bone–Vascular Axis
    Article Snippet: Identification of Vascular Sclerostin mRNA Total mRNA was extracted from the distal part of the abdominal aorta using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) and complementary DNA was generated with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR amplification was performed based on the TaqMan fluorescence methodology (ABI Prism® 7000 Sequence Detection System, Applied Biosystems).

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results.

    Article Title: Type I Interferon: Potential Therapeutic Target for Psoriasis?
    Article Snippet: Total RNA extraction and microarray processing Total RNA was extracted from PAXgene blood and skin biopsies using the PAXgene Blood RNA kit and the Qiagen RNeasy Fibrous Tissue Mini kit (Hilden, Germany), respectively. .. The generation of biotin-labeled amplified cRNA from 2 µg of total RNA was accomplished with the Affymetrix GeneChip® One-Cycle cDNA Synthesis kit and the Affymetrix GeneChip® IVT Labeling kit.

    Article Title: mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4
    Article Snippet: Transcript expression analyses Total RNA was extracted with the RNeasy Fibrous Tissue Mini Kit (Qiagen). .. Quantitative PCR was performed on DNAse-treated RNA, reverse transcribed to cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen), amplified with the Applied Biosystem Power Sybr Green Master Mix.

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol. .. The PCR amplification was performed using Fast SYBR green master mix (Life Technologies) and StepOne Plus (Life Technologies).

    Article Title: Canagliflozin, a sodium–glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Article Snippet: Messenger ribonucleic acid quantification Total ribonucleic acid (RNA) was isolated from frozen tissues by using an RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA, USA). .. DNA amplification was carried out in ABI PRISM7500 (Life Technologies) by using Taqman Universal PCR Master Mix.

    Synthesized:

    Article Title: The Effects of Prednisolone Treatment on Cytokine Expression in Patients with Erythema Nodosum Leprosum Reactions
    Article Snippet: RNA Isolation and Reverse Transcription Isolation of RNA from whole blood and skin lesion biopsies stored in RNAlater™ (Ambion, Austin, TX, USA) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom), respectively, according to the manufacturer’s protocol. .. For all samples, complementary DNA (cDNA was synthesized on the same day to avoid the risk of RNA degrades during storage). cDNA was synthesized from RNA (200 ng/reaction mixture) using High Capacity cDNA Reverse Transcriptase Kit (AB Applied Biosystems, UK).

    Article Title: Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p
    Article Snippet: Total RNA was extracted using the Rneasy Fibrous Tissue mini kit (Qiagen, Germany). .. The first-strand cDNA was synthesized from 2 μg of total RNA using the Omniscript reverse transcriptase kit (Qiagen, Germany).

    Article Title: Canagliflozin, a sodium–glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Article Snippet: Messenger ribonucleic acid quantification Total ribonucleic acid (RNA) was isolated from frozen tissues by using an RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA, USA). .. First‐strand complementary deoxyribonucleic acid (DNA) was synthesized using a SuperScript VILO™ cDNA Synthesis Kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA).

    Quantitative RT-PCR:

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: Paragraph title: Real-Time RT-PCR analysis ... RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA).

    Article Title: Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation
    Article Snippet: .. 2.3.2 qRT-PCR Total RNA was extracted using an RNeasy Fibrous kit (Qiagen) and ≤1 µg of RNA was used to synthesise cDNA (Applied Biosystems). .. TaqMan® RT-PCR was performed using the following TaqMan® Gene Expression Assays: IL-17A (Mm00439619_m1), IFNγ (Mm01168134_m1), IL-4 (Mm00445259_m1), IL-22 (Mm01226722_g1), TNFα (Mm00443259_g1) and IL-6 (Mm00446190m1), all from Applied Biosystems.

    Article Title: Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p
    Article Snippet: Paragraph title: qRT-PCR ... Total RNA was extracted using the Rneasy Fibrous Tissue mini kit (Qiagen, Germany).

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol. .. For quantitative RT-PCR, total RNA was reverse transcribed into first-strand complementary DNA (cDNA) using Super Script VILO (Life Technologies, Maryland, USA) and random primers.

    Real-time Polymerase Chain Reaction:

    Article Title: Sclerostin as Regulatory Molecule in Vascular Media Calcification and the Bone–Vascular Axis
    Article Snippet: Identification of Vascular Sclerostin mRNA Total mRNA was extracted from the distal part of the abdominal aorta using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) and complementary DNA was generated with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR amplification was performed based on the TaqMan fluorescence methodology (ABI Prism® 7000 Sequence Detection System, Applied Biosystems).

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results.

    Article Title: Cathepsin S Deficiency Mitigated Chronic Stress–Related Neointimal Hyperplasia in Mice
    Article Snippet: Gene Expression Assay Total RNA was extracted from the left and right carotid arteries of mice with the use of an RNeasy Fibrous Tissue Mini‐Kit (Qiagen, Hilden, Germany) and subjected to reverse transcription. .. The resulting cDNA was subjected to a quantitative real‐time polymerase reaction chain (PCR) analysis with primers specific for angiotensin II receptor 1 (ATR1), matrix metalloproteinase (MMP)‐9, MMP‐2, CatS, cathepsin L (CatL), intercellular adhesion molecule‐1 (ICAM‐1), vascular cell adhesion molecule‐1 (VCAM‐1), monocyte chemoattractant protein‐1 (MCP‐1), p22phox , p47phox , p67phox , gp91phox , nicotinamide‐adenine dinucleotide phosphate, reduced form (NADPH), oxidase 1, stromal cell–derived factor‐1 (SDF‐1α), C‐X‐C chemokine receptor‐4 (CXCR4), toll‐like receptor‐4 (TLR‐4), TLR‐2, tumor necrosis factor‐α (TNF‐α), and interleukin‐1β (IL‐1β) with the use of an ABI 7300 Real‐Time PCR System (Applied Biosystems, Foster City, CA).

    Article Title: mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4
    Article Snippet: Transcript expression analyses Total RNA was extracted with the RNeasy Fibrous Tissue Mini Kit (Qiagen). .. Quantitative PCR was performed on DNAse-treated RNA, reverse transcribed to cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen), amplified with the Applied Biosystem Power Sybr Green Master Mix.

    Microarray:

    Article Title: Type I Interferon: Potential Therapeutic Target for Psoriasis?
    Article Snippet: .. Total RNA extraction and microarray processing Total RNA was extracted from PAXgene blood and skin biopsies using the PAXgene Blood RNA kit and the Qiagen RNeasy Fibrous Tissue Mini kit (Hilden, Germany), respectively. .. RNA purity and concentration were determined spectrophotometrically (260/280 > 1.9).

    Article Title: COPD is accompanied by co-ordinated transcriptional perturbation in the quadriceps affecting the mitochondria and extracellular matrix
    Article Snippet: Gene expression profiling Total RNA was extracted from frozen quadriceps specimens stored at −80 °C using the RNeasy fibrous tissue kit (Qiagen). .. RNA was prepared for microarray analysis (98 COPD and 19 healthy controls) using the WT expression kit (Ambion) and GeneChip WT terminal labelling and hybridisation kit (Affymetrix).

    Incubation:

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Prior to total RNA extraction, divided cartilage tissues were minced and digested with 0.3 U/mL collagenase NB4 in 10 % FBS-DMEM and incubated overnight at 37 °C. .. Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol.

    Expressing:

    Article Title: Sclerostin as Regulatory Molecule in Vascular Media Calcification and the Bone–Vascular Axis
    Article Snippet: Identification of Vascular Sclerostin mRNA Total mRNA was extracted from the distal part of the abdominal aorta using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) and complementary DNA was generated with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). .. TaqMan probes and primers for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Rn99999916_s1) and sclerostin (SOST) (Rn00577971_m1) were purchased from Applied Biosystems as a Taqman® gene expression assay on demand.

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results.

    Article Title: Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation
    Article Snippet: 2.3.2 qRT-PCR Total RNA was extracted using an RNeasy Fibrous kit (Qiagen) and ≤1 µg of RNA was used to synthesise cDNA (Applied Biosystems). .. TaqMan® RT-PCR was performed using the following TaqMan® Gene Expression Assays: IL-17A (Mm00439619_m1), IFNγ (Mm01168134_m1), IL-4 (Mm00445259_m1), IL-22 (Mm01226722_g1), TNFα (Mm00443259_g1) and IL-6 (Mm00446190m1), all from Applied Biosystems.

    Article Title: Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p
    Article Snippet: Total RNA was extracted using the Rneasy Fibrous Tissue mini kit (Qiagen, Germany). .. Expression profiles of the two transcript isoforms of MSRB3 were determined using the SYBR Green method. qRT-PCR was performed in a 20-μL reaction volume containing 50 ng of template cDNA, 1 × Power SYBR Green Master mix (Applied Biosystems, USA) and 5 pM of each primer, with the following thermocycling conditions: 15 min at 95 °C and 48 cycles (95 °C for 15 s, 60 °C for 1 min and 72 °C for 1 min).

    Article Title: Cathepsin S Deficiency Mitigated Chronic Stress–Related Neointimal Hyperplasia in Mice
    Article Snippet: .. Gene Expression Assay Total RNA was extracted from the left and right carotid arteries of mice with the use of an RNeasy Fibrous Tissue Mini‐Kit (Qiagen, Hilden, Germany) and subjected to reverse transcription. .. The resulting cDNA was subjected to a quantitative real‐time polymerase reaction chain (PCR) analysis with primers specific for angiotensin II receptor 1 (ATR1), matrix metalloproteinase (MMP)‐9, MMP‐2, CatS, cathepsin L (CatL), intercellular adhesion molecule‐1 (ICAM‐1), vascular cell adhesion molecule‐1 (VCAM‐1), monocyte chemoattractant protein‐1 (MCP‐1), p22phox , p47phox , p67phox , gp91phox , nicotinamide‐adenine dinucleotide phosphate, reduced form (NADPH), oxidase 1, stromal cell–derived factor‐1 (SDF‐1α), C‐X‐C chemokine receptor‐4 (CXCR4), toll‐like receptor‐4 (TLR‐4), TLR‐2, tumor necrosis factor‐α (TNF‐α), and interleukin‐1β (IL‐1β) with the use of an ABI 7300 Real‐Time PCR System (Applied Biosystems, Foster City, CA).

    Article Title: mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4
    Article Snippet: .. Transcript expression analyses Total RNA was extracted with the RNeasy Fibrous Tissue Mini Kit (Qiagen). .. Quantitative PCR was performed on DNAse-treated RNA, reverse transcribed to cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen), amplified with the Applied Biosystem Power Sybr Green Master Mix.

    Article Title: COPD is accompanied by co-ordinated transcriptional perturbation in the quadriceps affecting the mitochondria and extracellular matrix
    Article Snippet: .. Gene expression profiling Total RNA was extracted from frozen quadriceps specimens stored at −80 °C using the RNeasy fibrous tissue kit (Qiagen). .. RNA quality was determined by Bioanalyzer (Agilent), and 5 samples with a RIN (RNA Integrity Number) < 6 were excluded.

    Article Title: Canagliflozin, a sodium–glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Article Snippet: Messenger ribonucleic acid quantification Total ribonucleic acid (RNA) was isolated from frozen tissues by using an RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA, USA). .. Taqman gene expression assays are shown in Table .

    Hybridization:

    Article Title: COPD is accompanied by co-ordinated transcriptional perturbation in the quadriceps affecting the mitochondria and extracellular matrix
    Article Snippet: Gene expression profiling Total RNA was extracted from frozen quadriceps specimens stored at −80 °C using the RNeasy fibrous tissue kit (Qiagen). .. RNA was prepared for microarray analysis (98 COPD and 19 healthy controls) using the WT expression kit (Ambion) and GeneChip WT terminal labelling and hybridisation kit (Affymetrix).

    Sequencing:

    Article Title: Sclerostin as Regulatory Molecule in Vascular Media Calcification and the Bone–Vascular Axis
    Article Snippet: Identification of Vascular Sclerostin mRNA Total mRNA was extracted from the distal part of the abdominal aorta using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) and complementary DNA was generated with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR amplification was performed based on the TaqMan fluorescence methodology (ABI Prism® 7000 Sequence Detection System, Applied Biosystems).

    Article Title: Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation
    Article Snippet: 2.3.2 qRT-PCR Total RNA was extracted using an RNeasy Fibrous kit (Qiagen) and ≤1 µg of RNA was used to synthesise cDNA (Applied Biosystems). .. Polymerase chain reactions were performed in duplicate in a StepOne sequence detector (Applied Biosystems).

    Cell Culture:

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol. .. The extraction of total RNA from human cultured chondrocytes was performed using NucleoSpin XS (Takara, Shiga, Japan) according to the manufacturer’s protocol.

    Generated:

    Article Title: Sclerostin as Regulatory Molecule in Vascular Media Calcification and the Bone–Vascular Axis
    Article Snippet: .. Identification of Vascular Sclerostin mRNA Total mRNA was extracted from the distal part of the abdominal aorta using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) and complementary DNA was generated with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR amplification was performed based on the TaqMan fluorescence methodology (ABI Prism® 7000 Sequence Detection System, Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. 2 µl of cDNA dilution (1:5 for ERα and ERβ, and 1:25 for α-actin) of reverse transcription (RT) product was applied to 20 µl RT-PCR reaction.

    Article Title: Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation
    Article Snippet: 2.3.2 qRT-PCR Total RNA was extracted using an RNeasy Fibrous kit (Qiagen) and ≤1 µg of RNA was used to synthesise cDNA (Applied Biosystems). .. TaqMan® RT-PCR was performed using the following TaqMan® Gene Expression Assays: IL-17A (Mm00439619_m1), IFNγ (Mm01168134_m1), IL-4 (Mm00445259_m1), IL-22 (Mm01226722_g1), TNFα (Mm00443259_g1) and IL-6 (Mm00446190m1), all from Applied Biosystems.

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, quantitative reverse transcription polymerase chain reaction (RT-PCR) ... Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol.

    Fluorescence:

    Article Title: Sclerostin as Regulatory Molecule in Vascular Media Calcification and the Bone–Vascular Axis
    Article Snippet: Identification of Vascular Sclerostin mRNA Total mRNA was extracted from the distal part of the abdominal aorta using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) and complementary DNA was generated with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR amplification was performed based on the TaqMan fluorescence methodology (ABI Prism® 7000 Sequence Detection System, Applied Biosystems).

    Isolation:

    Article Title: The Effects of Prednisolone Treatment on Cytokine Expression in Patients with Erythema Nodosum Leprosum Reactions
    Article Snippet: .. RNA Isolation and Reverse Transcription Isolation of RNA from whole blood and skin lesion biopsies stored in RNAlater™ (Ambion, Austin, TX, USA) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom), respectively, according to the manufacturer’s protocol. .. DNase I (QIAGEN) was included for all RNA preparations for DNA digestion.

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: .. RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. 1 μg of total RNA was used for reverse transcription to synthesize single-strand cDNA in a 20 µl-reaction mixture according to the protocol of First-Strand cDNA Synthesis Kit (Amersham Biosciences, Pittsburgh, PA).

    Article Title: Canagliflozin, a sodium–glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Article Snippet: .. Messenger ribonucleic acid quantification Total ribonucleic acid (RNA) was isolated from frozen tissues by using an RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA, USA). .. First‐strand complementary deoxyribonucleic acid (DNA) was synthesized using a SuperScript VILO™ cDNA Synthesis Kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA).

    Multiplex Assay:

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results.

    Labeling:

    Article Title: Type I Interferon: Potential Therapeutic Target for Psoriasis?
    Article Snippet: Total RNA extraction and microarray processing Total RNA was extracted from PAXgene blood and skin biopsies using the PAXgene Blood RNA kit and the Qiagen RNeasy Fibrous Tissue Mini kit (Hilden, Germany), respectively. .. The generation of biotin-labeled amplified cRNA from 2 µg of total RNA was accomplished with the Affymetrix GeneChip® One-Cycle cDNA Synthesis kit and the Affymetrix GeneChip® IVT Labeling kit.

    Mouse Assay:

    Article Title: Cathepsin S Deficiency Mitigated Chronic Stress–Related Neointimal Hyperplasia in Mice
    Article Snippet: .. Gene Expression Assay Total RNA was extracted from the left and right carotid arteries of mice with the use of an RNeasy Fibrous Tissue Mini‐Kit (Qiagen, Hilden, Germany) and subjected to reverse transcription. .. The resulting cDNA was subjected to a quantitative real‐time polymerase reaction chain (PCR) analysis with primers specific for angiotensin II receptor 1 (ATR1), matrix metalloproteinase (MMP)‐9, MMP‐2, CatS, cathepsin L (CatL), intercellular adhesion molecule‐1 (ICAM‐1), vascular cell adhesion molecule‐1 (VCAM‐1), monocyte chemoattractant protein‐1 (MCP‐1), p22phox , p47phox , p67phox , gp91phox , nicotinamide‐adenine dinucleotide phosphate, reduced form (NADPH), oxidase 1, stromal cell–derived factor‐1 (SDF‐1α), C‐X‐C chemokine receptor‐4 (CXCR4), toll‐like receptor‐4 (TLR‐4), TLR‐2, tumor necrosis factor‐α (TNF‐α), and interleukin‐1β (IL‐1β) with the use of an ABI 7300 Real‐Time PCR System (Applied Biosystems, Foster City, CA).

    Polymerase Chain Reaction:

    Article Title: Cathepsin S Deficiency Mitigated Chronic Stress–Related Neointimal Hyperplasia in Mice
    Article Snippet: Gene Expression Assay Total RNA was extracted from the left and right carotid arteries of mice with the use of an RNeasy Fibrous Tissue Mini‐Kit (Qiagen, Hilden, Germany) and subjected to reverse transcription. .. The resulting cDNA was subjected to a quantitative real‐time polymerase reaction chain (PCR) analysis with primers specific for angiotensin II receptor 1 (ATR1), matrix metalloproteinase (MMP)‐9, MMP‐2, CatS, cathepsin L (CatL), intercellular adhesion molecule‐1 (ICAM‐1), vascular cell adhesion molecule‐1 (VCAM‐1), monocyte chemoattractant protein‐1 (MCP‐1), p22phox , p47phox , p67phox , gp91phox , nicotinamide‐adenine dinucleotide phosphate, reduced form (NADPH), oxidase 1, stromal cell–derived factor‐1 (SDF‐1α), C‐X‐C chemokine receptor‐4 (CXCR4), toll‐like receptor‐4 (TLR‐4), TLR‐2, tumor necrosis factor‐α (TNF‐α), and interleukin‐1β (IL‐1β) with the use of an ABI 7300 Real‐Time PCR System (Applied Biosystems, Foster City, CA).

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol. .. The PCR amplification was performed using Fast SYBR green master mix (Life Technologies) and StepOne Plus (Life Technologies).

    Article Title: Canagliflozin, a sodium–glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Article Snippet: Messenger ribonucleic acid quantification Total ribonucleic acid (RNA) was isolated from frozen tissues by using an RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA, USA). .. DNA amplification was carried out in ABI PRISM7500 (Life Technologies) by using Taqman Universal PCR Master Mix.

    IA:

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results.

    Software:

    Article Title: Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation
    Article Snippet: 2.3.2 qRT-PCR Total RNA was extracted using an RNeasy Fibrous kit (Qiagen) and ≤1 µg of RNA was used to synthesise cDNA (Applied Biosystems). .. Data analysis was performed using the Applied Biosystems sequence detection software and samples were normalised to the reference reporter mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Mm99999915_g1) endogenous control.

    Article Title: mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4
    Article Snippet: Transcript expression analyses Total RNA was extracted with the RNeasy Fibrous Tissue Mini Kit (Qiagen). .. Data were analyzed using StepOne software and normalized to Tbp expression.

    SYBR Green Assay:

    Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
    Article Snippet: RNA was isolated from endothelium-denuded aortic tissue samples using RNeasy Fibrous Tissue Mini Kit (QIAGEN, Valencia, CA). .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results.

    Article Title: Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p
    Article Snippet: Total RNA was extracted using the Rneasy Fibrous Tissue mini kit (Qiagen, Germany). .. Expression profiles of the two transcript isoforms of MSRB3 were determined using the SYBR Green method. qRT-PCR was performed in a 20-μL reaction volume containing 50 ng of template cDNA, 1 × Power SYBR Green Master mix (Applied Biosystems, USA) and 5 pM of each primer, with the following thermocycling conditions: 15 min at 95 °C and 48 cycles (95 °C for 15 s, 60 °C for 1 min and 72 °C for 1 min).

    Article Title: mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4
    Article Snippet: Transcript expression analyses Total RNA was extracted with the RNeasy Fibrous Tissue Mini Kit (Qiagen). .. Quantitative PCR was performed on DNAse-treated RNA, reverse transcribed to cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen), amplified with the Applied Biosystem Power Sybr Green Master Mix.

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol. .. The PCR amplification was performed using Fast SYBR green master mix (Life Technologies) and StepOne Plus (Life Technologies).

    RNA Extraction:

    Article Title: Type I Interferon: Potential Therapeutic Target for Psoriasis?
    Article Snippet: .. Total RNA extraction and microarray processing Total RNA was extracted from PAXgene blood and skin biopsies using the PAXgene Blood RNA kit and the Qiagen RNeasy Fibrous Tissue Mini kit (Hilden, Germany), respectively. .. RNA purity and concentration were determined spectrophotometrically (260/280 > 1.9).

    Article Title: Expression and pathological effects of periostin in human osteoarthritis cartilage
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, quantitative reverse transcription polymerase chain reaction (RT-PCR) ... Synovial tissues were thoroughly minced and preserved at −80 °C, after which extraction of total RNA from the clinical sample was performed using an RNeasy fibrous kit (QIAGEN, Hilden, Netherland) according to the manufacturer’s protocol.

    Agarose Gel Electrophoresis:

    Article Title: The Effects of Prednisolone Treatment on Cytokine Expression in Patients with Erythema Nodosum Leprosum Reactions
    Article Snippet: RNA Isolation and Reverse Transcription Isolation of RNA from whole blood and skin lesion biopsies stored in RNAlater™ (Ambion, Austin, TX, USA) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom), respectively, according to the manufacturer’s protocol. .. RNA yield was determined using a NanoDrop 2000, spectrophotometer (Thermo Scientific, Epsom, UK) and integrity was checked by agarose gel electrophoresis.

    Spectrophotometry:

    Article Title: The Effects of Prednisolone Treatment on Cytokine Expression in Patients with Erythema Nodosum Leprosum Reactions
    Article Snippet: RNA Isolation and Reverse Transcription Isolation of RNA from whole blood and skin lesion biopsies stored in RNAlater™ (Ambion, Austin, TX, USA) was performed using PAXgene Blood RNA Kit and RNeasy Fibrous Tissue Kit (QIAGEN Crawley, West Sussex, United Kingdom), respectively, according to the manufacturer’s protocol. .. RNA yield was determined using a NanoDrop 2000, spectrophotometer (Thermo Scientific, Epsom, UK) and integrity was checked by agarose gel electrophoresis.

    Concentration Assay:

    Article Title: Type I Interferon: Potential Therapeutic Target for Psoriasis?
    Article Snippet: Total RNA extraction and microarray processing Total RNA was extracted from PAXgene blood and skin biopsies using the PAXgene Blood RNA kit and the Qiagen RNeasy Fibrous Tissue Mini kit (Hilden, Germany), respectively. .. RNA purity and concentration were determined spectrophotometrically (260/280 > 1.9).

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  • 99
    Qiagen rneasy fibrous tissue minikit
    Rneasy Fibrous Tissue Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy fibrous tissue minikit/product/Qiagen
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    rneasy fibrous tissue minikit - by Bioz Stars, 2020-04
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