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Promega rnasin lcb promega
Rnasin Lcb Promega, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnasin lcb promega/product/Promega
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rnasin lcb promega - by Bioz Stars, 2020-03
79/100 stars

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Acrylamide Gel Assay:

Article Title: Kin28 Is Found within TFIIH and a Kin28-Ccl1-Tfb3 Trimer Complex with Differential Sensitivities to T-Loop Phosphorylation
Article Snippet: Transcription reaction mixtures (30 μl each) were assembled on ice and contained 12 μl of mixture A (25 mM HEPES-KOH {pH 7.8}, 10% glycerol, 100 mM KOAc {pH 7.8}, 10 mM Mg acetate {pH 7.8}, 5 mM EGTA, 2.5 mM DTT, 10 mM phosphocreatine {Sigma}, 1 U of creatine kinase {Sigma}, 10 U of RNasin {Promega}, 3 μl of deoxynucleoside triphosphate mixture {500 μM ATP and CTP, 10 μM UTP, 0.5 μl [α-32 P]UTP [NEN-Life Science Products], 10 μM 3"- o -methyl-GTP [Amersham Pharmacia Biotech]}), whole-cell extract (200 μg), 100 ng of plasmid template (pCYC-GAL4 CG− ), and 100 ng of activator (GAL4-VP16). .. RNA was resuspended in 10 μl of 0.1% SDS in 50% formamide and resolved on an acrylamide gel.

Incubation:

Article Title: Kin28 Is Found within TFIIH and a Kin28-Ccl1-Tfb3 Trimer Complex with Differential Sensitivities to T-Loop Phosphorylation
Article Snippet: Transcription reaction mixtures (30 μl each) were assembled on ice and contained 12 μl of mixture A (25 mM HEPES-KOH {pH 7.8}, 10% glycerol, 100 mM KOAc {pH 7.8}, 10 mM Mg acetate {pH 7.8}, 5 mM EGTA, 2.5 mM DTT, 10 mM phosphocreatine {Sigma}, 1 U of creatine kinase {Sigma}, 10 U of RNasin {Promega}, 3 μl of deoxynucleoside triphosphate mixture {500 μM ATP and CTP, 10 μM UTP, 0.5 μl [α-32 P]UTP [NEN-Life Science Products], 10 μM 3"- o -methyl-GTP [Amersham Pharmacia Biotech]}), whole-cell extract (200 μg), 100 ng of plasmid template (pCYC-GAL4 CG− ), and 100 ng of activator (GAL4-VP16). .. Reactions were allowed to proceed at 25°C for 45 to 60 min and were terminated by the addition of 120 μl of RNase T1 buffer (10 mM Tris-HCl [pH 7.5], 300 mM NaCl, 5 mM EDTA, 50 U of RNase T1 ), followed by incubation at room temperature for 15 min. SDS was then added to 0.5%, and proteinase K was added to 100 μg/ml, followed by incubation for 20 min at 37°C.

In Vitro:

Article Title: Kin28 Is Found within TFIIH and a Kin28-Ccl1-Tfb3 Trimer Complex with Differential Sensitivities to T-Loop Phosphorylation
Article Snippet: Paragraph title: In vitro transcription assays. ... Transcription reaction mixtures (30 μl each) were assembled on ice and contained 12 μl of mixture A (25 mM HEPES-KOH {pH 7.8}, 10% glycerol, 100 mM KOAc {pH 7.8}, 10 mM Mg acetate {pH 7.8}, 5 mM EGTA, 2.5 mM DTT, 10 mM phosphocreatine {Sigma}, 1 U of creatine kinase {Sigma}, 10 U of RNasin {Promega}, 3 μl of deoxynucleoside triphosphate mixture {500 μM ATP and CTP, 10 μM UTP, 0.5 μl [α-32 P]UTP [NEN-Life Science Products], 10 μM 3"- o -methyl-GTP [Amersham Pharmacia Biotech]}), whole-cell extract (200 μg), 100 ng of plasmid template (pCYC-GAL4 CG− ), and 100 ng of activator (GAL4-VP16).

Plasmid Preparation:

Article Title: Kin28 Is Found within TFIIH and a Kin28-Ccl1-Tfb3 Trimer Complex with Differential Sensitivities to T-Loop Phosphorylation
Article Snippet: .. Transcription reaction mixtures (30 μl each) were assembled on ice and contained 12 μl of mixture A (25 mM HEPES-KOH {pH 7.8}, 10% glycerol, 100 mM KOAc {pH 7.8}, 10 mM Mg acetate {pH 7.8}, 5 mM EGTA, 2.5 mM DTT, 10 mM phosphocreatine {Sigma}, 1 U of creatine kinase {Sigma}, 10 U of RNasin {Promega}, 3 μl of deoxynucleoside triphosphate mixture {500 μM ATP and CTP, 10 μM UTP, 0.5 μl [α-32 P]UTP [NEN-Life Science Products], 10 μM 3"- o -methyl-GTP [Amersham Pharmacia Biotech]}), whole-cell extract (200 μg), 100 ng of plasmid template (pCYC-GAL4 CG− ), and 100 ng of activator (GAL4-VP16). .. Reactions were allowed to proceed at 25°C for 45 to 60 min and were terminated by the addition of 120 μl of RNase T1 buffer (10 mM Tris-HCl [pH 7.5], 300 mM NaCl, 5 mM EDTA, 50 U of RNase T1 ), followed by incubation at room temperature for 15 min. SDS was then added to 0.5%, and proteinase K was added to 100 μg/ml, followed by incubation for 20 min at 37°C.

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    Promega rnasin lcb promega
    Rnasin Lcb Promega, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasin lcb promega/product/Promega
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnasin lcb promega - by Bioz Stars, 2020-03
    79/100 stars
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