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Promega rnasin enzyme
Rnasin Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnasin enzyme/product/Promega
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rnasin enzyme - by Bioz Stars, 2020-03
86/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. The reverse transcription reaction program consisted of three steps: 10 min at 25 °C, 50 min at 37 °C, and 15 min at 70 °C. mRNA expression was measured using a LightCycler 480 real-time PCR system and SYBR Green I Master mix® (Roche Diagnostics) (primers are listed in Additional file : Table S1).

Serial Dilution:

Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. Standard curves were generated for each run by serial dilution of control cDNA.

Generated:

Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. Standard curves were generated for each run by serial dilution of control cDNA.

SYBR Green Assay:

Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
Article Snippet: Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology). .. The reverse transcription reaction program consisted of three steps: 10 min at 25 °C, 50 min at 37 °C, and 15 min at 70 °C. mRNA expression was measured using a LightCycler 480 real-time PCR system and SYBR Green I Master mix® (Roche Diagnostics) (primers are listed in Additional file : Table S1).

Incubation:

Article Title: In Vivo and In Vitro Acquisition of Resistance to Voriconazole by Candida krusei
Article Snippet: Reverse transcriptase reactions were performed as recommended by the manufacturer; 50 ng of RNA sample, 0.015 ng/μl random primers (Invitrogen, Carlsbad, CA), 0.5 mM dNTPs (Fermentas, Vilnius, Lithuania), and RNase-free water up to 13 μl were incubated at 65°C for 5 min and then placed on ice for 1 min. .. Subsequently, 1× reverse transcriptase enzyme buffer (Invitrogen, Carlsbad, CA), 5 mM dithiothreitol (DTT) (Invitrogen), 1 U/μl RNasin enzyme (Promega, Madison, WI), 5 U/μl reverse transcriptase enzyme (Invitrogen), and RNase-free water were added up to a final volume of 20 μl.

Expressing:

Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
Article Snippet: Paragraph title: Gene expression ... Samples were then added to a mix containing 4 μl of first strand 5× buffer, 2 μl of 10× dithiothreitol, 1 μl of 10 mM dNTP mix, 300 ng/μl of hexaprimer (random primers), 20–40 U/μl of RNasin® enzyme (Promega), and 200 U/μl of MMLV-RT enzyme (Life Technology).

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  • 86
    Promega rnasin enzyme
    Rnasin Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasin enzyme/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnasin enzyme - by Bioz Stars, 2020-03
    86/100 stars
      Buy from Supplier

    83
    Promega rnasin promega enzymes
    Rnasin Promega Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasin promega enzymes/product/Promega
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnasin promega enzymes - by Bioz Stars, 2020-03
    83/100 stars
      Buy from Supplier

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