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Millipore rnaseout
Rnaseout, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaseout/product/Millipore
Average 93 stars, based on 10 article reviews
Price from $9.99 to $1999.99
rnaseout - by Bioz Stars, 2020-09
93/100 stars

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In Vitro:

Article Title: The in vitro RNA synthesizing activity of the isolated arterivirus replication/transcription complex is dependent on a host factor.
Article Snippet: .. In Vitro RNA Synthesis Assay (IVRA)—Following optimization of reaction conditions (as described under “Results”), standard 28- l IVRAmixtures contained 25 l of EAV-infected cell lysate (either PNS, S10, P10, or combinations), 30 mM HEPES, pH 7.4, 220 mM sucrose, 7 mM KCl, 2.5 mM DTT, 2.5 mM MgOAc, 2 g/ml ActD, 25 units of RNaseOUT, 20 mM creatine phosphate (Sigma), 10 units/ml creatine phosphokinase (Sigma), 1 mM ATP, 0.25 mM GTP, 0.25 mM UTP, 0.6 M CTP, and 0.12 M, 10 Ci of [ -32P]CTP (GE Healthcare). .. Unless otherwise indicated, standard reactions were performed 16526 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 283 • NUMBER 24 • JUNE 13, 2008 by guest on M ay 30, 2020 http://w w w .jbc.org/ D ow nloaded from for 100min at 30 °C.

Protease Inhibitor:

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum. .. The cells were immunostained with a primary antibody raised against puromycin, PMY-2A4 (1:100 dilution, developed by Dr. Jonathan Yewdell (National Institutes of Health) , and obtained from the Developmental Studies Hybridoma Bank, created by the NICHD, National Institutes of Health, and maintained at the University of Iowa Department of Biology), detected using Alexa Fluor 555-labeled anti-mouse secondary antibodies (1:200).

Incubation:

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation. .. The bead-protein-RNA complex was washed with ChIP dilution buffer containing 300 mM NaCl and 80 units/mL RNaseOUT.

Polymerase Chain Reaction:

Article Title: VSV-Displayed HIV-1 Envelope Identifies Broadly Neutralizing Antibodies Class-Switched to IgG and IgA
Article Snippet: .. After washing, cells were analyzed and sorted using a multi-laser MoFlo sorter (Beckman Coulter, Jersey City, NJ) contained with Biosafety Level 3 standards; single cells were sorted into 96-well PCR plates containing 20 μL lysis buffer, consisting of 5 μL 5 × first-strand buffer (Invitrogen), 1.25 μL 0.1 M DTT, 0.5 μL RNaseOut, and 0.0625 μL Igepal (Sigma, St. Louis, MO). .. The total content of cells passing through the sorter was analyzed with FlowJo (TreeStar, Cupertino, CA).

Lysis:

Article Title: VSV-Displayed HIV-1 Envelope Identifies Broadly Neutralizing Antibodies Class-Switched to IgG and IgA
Article Snippet: .. After washing, cells were analyzed and sorted using a multi-laser MoFlo sorter (Beckman Coulter, Jersey City, NJ) contained with Biosafety Level 3 standards; single cells were sorted into 96-well PCR plates containing 20 μL lysis buffer, consisting of 5 μL 5 × first-strand buffer (Invitrogen), 1.25 μL 0.1 M DTT, 0.5 μL RNaseOut, and 0.0625 μL Igepal (Sigma, St. Louis, MO). .. The total content of cells passing through the sorter was analyzed with FlowJo (TreeStar, Cupertino, CA).

Chromatin Immunoprecipitation:

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation. .. The bead-protein-RNA complex was washed with ChIP dilution buffer containing 300 mM NaCl and 80 units/mL RNaseOUT.

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    Millipore rnaseout
    Rnaseout, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout/product/Millipore
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rnaseout - by Bioz Stars, 2020-09
    93/100 stars
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