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Millipore rnaseout
Rnaseout, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaseout/product/Millipore
Average 95 stars, based on 4 article reviews
Price from $9.99 to $1999.99
rnaseout - by Bioz Stars, 2020-02
95/100 stars

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Clone Assay:

Article Title: Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
Article Snippet: Paragraph title: Single-Cell PCR for Cloning mAbs ... The lysis buffer was composed of 40 U RNaseOUT, 6.25 mM dithiothreitol (DTT), 5 μl 5 × First-Strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl2 ), and 0.0625 μl Igepal (Sigma).

Centrifugation:

Article Title: Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative "Futile" Cycle *
Article Snippet: Cells were harvested by centrifugation, and the cells were washed twice in 25 m m Hepes-KOH (pH 7.5), 150 m m KCl, 2 m m MgCl2 . .. Cells were resuspended in the above buffer containing 20 m m vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 m m DTT with protease inhibitor mixture.

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: Cells were harvested by centrifugation and the cells were washed twice in 25 mM HEPES-KOH (pH 7.5), 150 mM KCl, 2 mM MgCl2 . .. Cells were resuspended in the above buffer containing 20 mM vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 mM DTT, with protease inhibitor cocktail.

Amplification:

Article Title: Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
Article Snippet: The lysis buffer was composed of 40 U RNaseOUT, 6.25 mM dithiothreitol (DTT), 5 μl 5 × First-Strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl2 ), and 0.0625 μl Igepal (Sigma). .. Antibody genes were amplified from the cDNA by nested PCR, using previously described IgG heavy and light chain-specific primers , with some modifications ( , ).

Microarray:

Article Title: Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative "Futile" Cycle *
Article Snippet: Paragraph title: RNA Immunoprecipitation and Microarray Analysis ... Cells were resuspended in the above buffer containing 20 m m vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 m m DTT with protease inhibitor mixture.

Incubation:

Article Title: Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Article Snippet: For the total reaction: the GSP-TAG, 0.5mM dNTP, 5mM MgCl2, 10mM DTT, 40U RNaseOUT, 100U SuperScriptIII® RT and 240ng Actinomycin D (Sigma-Aldrich, St. Louis, MO) were added with the RNA for a 20μl reaction. .. After cDNA synthesis 2U recombinant RNase H was added to each reaction and incubated 20 min at 37°C.

Article Title: Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative "Futile" Cycle *
Article Snippet: Briefly, protein G-Sepharose (Sigma) was washed three times with 50 m m Tris-HCl (pH 8.0), 150 m m NaCl, 0.1% Nonidet P-40, and 30 μg of anti- myc monoclonal antibodies (Sigma) was added and incubated for 1 h at 4 °C. .. Cells were resuspended in the above buffer containing 20 m m vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 m m DTT with protease inhibitor mixture.

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: Briefly, protein G-Sepharose (Sigma) was washed 3 times with 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.1% NP40, and either 30 μg of anti-myc monoclonal antibodies (Sigma) or anti-FLAG monoclonal (Sigma) was added and incubated for 1 h at 4°C. .. Cells were resuspended in the above buffer containing 20 mM vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 mM DTT, with protease inhibitor cocktail.

Article Title: Differential Gene Expression in Response to Salinity and Temperature in a Haloarcula Strain from Great Salt Lake, Utah
Article Snippet: This mixture was incubated in the absence of buffer at 75 °C for 3 min in order to remove or denature the secondary structure of RNA, and then immediately placed on ice. .. Subsequently the following were added to each reaction: 5× complementary DNA (cDNA) synthesis buffer, 0.1 M DTT, RNaseOUT (40 units/μL), Thermosript RT (15 U/μL), 5 M betaine (Sigma Aldrich), and RNase free water to a total volume of 20 μL.

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation. .. The bead-protein-RNA complex was washed with ChIP dilution buffer containing 300 mM NaCl and 80 units/mL RNaseOUT.

Article Title: Inflammatory and Metabolic Alterations of Kager's Fat Pad in Chronic Achilles Tendinopathy
Article Snippet: Reactions were performed in 25 μl containing 1x 1st Strand Buffer (Life Technologies), 0.1 μg random hexamers (Bioline), 0.9 mM of each dNTP (Sigma-Aldrich), 20 units of RNaseOUT (Sigma-Aldrich), 1 μg of total DNase-treated RNA and 200 units of Moloney murine leukaemia virus reverse transcriptase (Life Technologies). .. Reactions were left for 10 min at room temperature, followed by incubation at 37°C for 1 h. After cDNA synthesis, reactions were diluted with 50 μl of nuclease-free water and frozen at -80°C.

Article Title: Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya
Article Snippet: Lyophilization of RT-LAMP reagents A mixture of Bst 2.0 WarmStart® DNA Polymerase (16 U), WarmStart® RTx Reverse Transcriptase (15 U), RNaseOUT™ (80 U) and Antarctic thermolabile UDG (2 U) in dialysis buffer (200 μL, 10 mM Tris-HCl pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA10 and 0.1% Triton X-100) was placed in an ultrafiltration membrane (10 kDA cut-off limit, Millipore, Billerica, MA). .. Plasma samples (5 μL) were mixed with 1.1X rehydration buffer (45 μL) and incubated at 65 °C for 45 min.

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: For the ribopuromycylation assay, the cells were incubated for 15 min at 37 °C in a humidified 5% CO2 atmosphere with culture medium supplemented with 91 μ m puromycin and 208 μ m emetine, with one well not including puromycin as a negative control. .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum.

Activity Assay:

Article Title: Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Article Snippet: For the total reaction: the GSP-TAG, 0.5mM dNTP, 5mM MgCl2, 10mM DTT, 40U RNaseOUT, 100U SuperScriptIII® RT and 240ng Actinomycin D (Sigma-Aldrich, St. Louis, MO) were added with the RNA for a 20μl reaction. .. Synthesis was performed at 50°C for 50 min and terminated at 85°C for 5 min. RT with extremely low intrinsic RNase H activity (for cleavage of RNA from RNA/DNA duplexes) and Actinomycin D was added to prevent second strand cDNA RT resulting in antisense artifacts .

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: Extracellular Cn was washed away with RAW264.7 culture medium, and macrophage ribosome activity was determined by two separate methods: ribopuromycylation, adapted from Coelho et al. , and staining with O- propargyl-puromycin using the Click-iT Plus OPP protein synthesis assay kit Alexa Fluor 647 (Life Technologies) in accordance with the manufacturer's guidelines. .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum.

Expressing:

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: RNA and Protein Immunoprecipitation RNA Immunoprecipitation was performed by an adaptation of a previously described method using strains expressing c-myc -Vad1 or 3XFLAG-Dcp2. .. Cells were resuspended in the above buffer containing 20 mM vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 mM DTT, with protease inhibitor cocktail.

Modification:

Article Title: Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
Article Snippet: Single-Cell PCR for Cloning mAbs Reverse transcription and single-cell IgG cloning were carried out as previously described , with a small modification. .. The lysis buffer was composed of 40 U RNaseOUT, 6.25 mM dithiothreitol (DTT), 5 μl 5 × First-Strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl2 ), and 0.0625 μl Igepal (Sigma).

Protease Inhibitor:

Article Title: Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative "Futile" Cycle *
Article Snippet: .. Cells were resuspended in the above buffer containing 20 m m vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 m m DTT with protease inhibitor mixture. ..

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: .. Cells were resuspended in the above buffer containing 20 mM vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 mM DTT, with protease inhibitor cocktail. ..

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum. .. The cells were immunostained with a primary antibody raised against puromycin, PMY-2A4 (1:100 dilution, developed by Dr. Jonathan Yewdell (National Institutes of Health) , and obtained from the Developmental Studies Hybridoma Bank, created by the NICHD, National Institutes of Health, and maintained at the University of Iowa Department of Biology), detected using Alexa Fluor 555-labeled anti-mouse secondary antibodies (1:200).

Infection:

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: The cells were activated with 500 units/ml IFN-γ and 1 μg/ml LPS ∼16 h prior to infection with Cn and again with 1 μg/ml LPS concurrent with Cn infection. .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum.

Polymerase Chain Reaction:

Article Title: Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Article Snippet: Paragraph title: First strand cDNA synthesis and strand specific PCR for detection of sense and antisense ERV transcripts using TASA-TD methodology ... For the total reaction: the GSP-TAG, 0.5mM dNTP, 5mM MgCl2, 10mM DTT, 40U RNaseOUT, 100U SuperScriptIII® RT and 240ng Actinomycin D (Sigma-Aldrich, St. Louis, MO) were added with the RNA for a 20μl reaction.

Article Title: Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
Article Snippet: Paragraph title: Single-Cell PCR for Cloning mAbs ... The lysis buffer was composed of 40 U RNaseOUT, 6.25 mM dithiothreitol (DTT), 5 μl 5 × First-Strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl2 ), and 0.0625 μl Igepal (Sigma).

Article Title: Differential Gene Expression in Response to Salinity and Temperature in a Haloarcula Strain from Great Salt Lake, Utah
Article Snippet: Paragraph title: 2.7. RNA Extraction and Reverse Transcription PCR ... Subsequently the following were added to each reaction: 5× complementary DNA (cDNA) synthesis buffer, 0.1 M DTT, RNaseOUT (40 units/μL), Thermosript RT (15 U/μL), 5 M betaine (Sigma Aldrich), and RNase free water to a total volume of 20 μL.

Sonication:

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: The nuclei were resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8, 10 mM EDTA, and 1% SDS) containing 160 units/mL RNaseOUT followed by sonication. .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation.

Recombinant:

Article Title: Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Article Snippet: For the total reaction: the GSP-TAG, 0.5mM dNTP, 5mM MgCl2, 10mM DTT, 40U RNaseOUT, 100U SuperScriptIII® RT and 240ng Actinomycin D (Sigma-Aldrich, St. Louis, MO) were added with the RNA for a 20μl reaction. .. After cDNA synthesis 2U recombinant RNase H was added to each reaction and incubated 20 min at 37°C.

Fluorescence:

Article Title: Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya
Article Snippet: Lyophilization of RT-LAMP reagents A mixture of Bst 2.0 WarmStart® DNA Polymerase (16 U), WarmStart® RTx Reverse Transcriptase (15 U), RNaseOUT™ (80 U) and Antarctic thermolabile UDG (2 U) in dialysis buffer (200 μL, 10 mM Tris-HCl pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA10 and 0.1% Triton X-100) was placed in an ultrafiltration membrane (10 kDA cut-off limit, Millipore, Billerica, MA). .. The resulting fluorescence signal was observed by blue LED excitation (470 nm) through an orange filter.

Isolation:

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: The nuclei were collected by centrifuging at 3000 g for 10 min and washed once with nuclei isolation buffer II as used in ChIP containing 8 units/mL RNaseOUT. .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation.

Article Title: Inflammatory and Metabolic Alterations of Kager's Fat Pad in Chronic Achilles Tendinopathy
Article Snippet: Briefly, after homogenization in TRI Reagent, total RNA was isolated by phase-separation after addition of 0.2 ml chloroform. .. Reactions were performed in 25 μl containing 1x 1st Strand Buffer (Life Technologies), 0.1 μg random hexamers (Bioline), 0.9 mM of each dNTP (Sigma-Aldrich), 20 units of RNaseOUT (Sigma-Aldrich), 1 μg of total DNase-treated RNA and 200 units of Moloney murine leukaemia virus reverse transcriptase (Life Technologies).

RNA Extraction:

Article Title: Differential Gene Expression in Response to Salinity and Temperature in a Haloarcula Strain from Great Salt Lake, Utah
Article Snippet: Paragraph title: 2.7. RNA Extraction and Reverse Transcription PCR ... Subsequently the following were added to each reaction: 5× complementary DNA (cDNA) synthesis buffer, 0.1 M DTT, RNaseOUT (40 units/μL), Thermosript RT (15 U/μL), 5 M betaine (Sigma Aldrich), and RNase free water to a total volume of 20 μL.

Microscopy:

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum. .. With the Click-iT Plus OPP kit, one well was not infected with Cn and treated with 100 μ m of the ribosome inhibitor CHX for 30 min. Stained cells were imaged using a Zeiss LSM700 laser scanning confocal microscope.

Purification:

Article Title: Inflammatory and Metabolic Alterations of Kager's Fat Pad in Chronic Achilles Tendinopathy
Article Snippet: Paragraph title: RNA purification and reverse transcription ... Reactions were performed in 25 μl containing 1x 1st Strand Buffer (Life Technologies), 0.1 μg random hexamers (Bioline), 0.9 mM of each dNTP (Sigma-Aldrich), 20 units of RNaseOUT (Sigma-Aldrich), 1 μg of total DNase-treated RNA and 200 units of Moloney murine leukaemia virus reverse transcriptase (Life Technologies).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
Article Snippet: Components from the SuperScript III First-Strand Synthesis System for RT-PCR (Thermo Fisher Scientific, Waltham, MA) were adapted to perform reverse transcription with RNA from H69AR previously pulsed with IFN–γ 10 minutes. .. For the total reaction: the GSP-TAG, 0.5mM dNTP, 5mM MgCl2, 10mM DTT, 40U RNaseOUT, 100U SuperScriptIII® RT and 240ng Actinomycin D (Sigma-Aldrich, St. Louis, MO) were added with the RNA for a 20μl reaction.

Article Title: Differential Gene Expression in Response to Salinity and Temperature in a Haloarcula Strain from Great Salt Lake, Utah
Article Snippet: Reverse transcription PCR (RT-PCR) was performed with three 13-mer arbitrary primers ( ) and Thermoscript Reverse Transcriptase (Invitrogen). .. Subsequently the following were added to each reaction: 5× complementary DNA (cDNA) synthesis buffer, 0.1 M DTT, RNaseOUT (40 units/μL), Thermosript RT (15 U/μL), 5 M betaine (Sigma Aldrich), and RNase free water to a total volume of 20 μL.

Lysis:

Article Title: Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
Article Snippet: .. The lysis buffer was composed of 40 U RNaseOUT, 6.25 mM dithiothreitol (DTT), 5 μl 5 × First-Strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl2 ), and 0.0625 μl Igepal (Sigma). .. Next, 150 ng random hexamers (Takara), 0.4 mM dNTPs (Fermentas), and 50 U Superscript III (Invitrogen) was added, with a total volume of 5 μl. cDNA synthesis was performed for 50 min at 50°C, 5 min at 85°C, and cooling at 4°C.

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: The nuclei were resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8, 10 mM EDTA, and 1% SDS) containing 160 units/mL RNaseOUT followed by sonication. .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation.

Nested PCR:

Article Title: Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
Article Snippet: The lysis buffer was composed of 40 U RNaseOUT, 6.25 mM dithiothreitol (DTT), 5 μl 5 × First-Strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl2 ), and 0.0625 μl Igepal (Sigma). .. Antibody genes were amplified from the cDNA by nested PCR, using previously described IgG heavy and light chain-specific primers , with some modifications ( , ).

Chromatin Immunoprecipitation:

Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
Article Snippet: .. After centrifuging at 16,000 g for 10 min, the supernatant was diluted 10× with ChIP dilution buffer as used in ChIP containing 160 units/mL RNaseOUT and incubated with anti-FLAG M2 beads (Sigma-Aldrich; M8823) for 3 h with rotation. .. The bead-protein-RNA complex was washed with ChIP dilution buffer containing 300 mM NaCl and 80 units/mL RNaseOUT.

Negative Control:

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: For the ribopuromycylation assay, the cells were incubated for 15 min at 37 °C in a humidified 5% CO2 atmosphere with culture medium supplemented with 91 μ m puromycin and 208 μ m emetine, with one well not including puromycin as a negative control. .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum.

In Vitro:

Article Title: SARS-Coronavirus Replication/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro
Article Snippet: Paragraph title: In vitro RNA synthesis assay (IVRA) ... The subsequent addition of reaction components yielded a 28 µl final reaction volume, containing 30 mM HEPES pH 7.4, 220 mM sucrose, 7 mM KCl, 2.5 mM DTT, 2 mM MgOAc2 , 2 µg/ml actinomycin D, 25 U RNaseOUT, 20 mM creatine phosphate (Sigma), 10 U/ml creatine phosphokinase (Sigma), 1 mM ATP, 0.25 mM GTP, 0.25 mM UTP, 0.6 µM CTP and 0.12 µM and 10 µCi [α-32 P]CTP (GE Healthcare).

Homogenization:

Article Title: Inflammatory and Metabolic Alterations of Kager's Fat Pad in Chronic Achilles Tendinopathy
Article Snippet: Briefly, after homogenization in TRI Reagent, total RNA was isolated by phase-separation after addition of 0.2 ml chloroform. .. Reactions were performed in 25 μl containing 1x 1st Strand Buffer (Life Technologies), 0.1 μg random hexamers (Bioline), 0.9 mM of each dNTP (Sigma-Aldrich), 20 units of RNaseOUT (Sigma-Aldrich), 1 μg of total DNase-treated RNA and 200 units of Moloney murine leukaemia virus reverse transcriptase (Life Technologies).

Spectrophotometry:

Article Title: Differential Gene Expression in Response to Salinity and Temperature in a Haloarcula Strain from Great Salt Lake, Utah
Article Snippet: Total RNA was visualized and quantified with a UV spectrophotometer. .. Subsequently the following were added to each reaction: 5× complementary DNA (cDNA) synthesis buffer, 0.1 M DTT, RNaseOUT (40 units/μL), Thermosript RT (15 U/μL), 5 M betaine (Sigma Aldrich), and RNase free water to a total volume of 20 μL.

Immunoprecipitation:

Article Title: Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative "Futile" Cycle *
Article Snippet: Paragraph title: RNA Immunoprecipitation and Microarray Analysis ... Cells were resuspended in the above buffer containing 20 m m vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 m m DTT with protease inhibitor mixture.

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: Paragraph title: RNA and Protein Immunoprecipitation ... Cells were resuspended in the above buffer containing 20 mM vanadyl ribonucleoside complexes (Sigma), 200 units/ml RNaseOUT (Sigma), 0.1% Nonidet P-40, 1 mM DTT, with protease inhibitor cocktail.

Staining:

Article Title: Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans *
Article Snippet: Extracellular Cn was washed away with RAW264.7 culture medium, and macrophage ribosome activity was determined by two separate methods: ribopuromycylation, adapted from Coelho et al. , and staining with O- propargyl-puromycin using the Click-iT Plus OPP protein synthesis assay kit Alexa Fluor 647 (Life Technologies) in accordance with the manufacturer's guidelines. .. The cells were permeabilized with 50 m m Tris-HCl, 10 units/ml RNaseOut, 0.015% digitonin, cOmpleteTM Mini Protease Inhibitor Mixture tablet (Sigma-Aldrich), fixed with 3% paraformaldehyde, and blocked with 1× PBS, 0.05% saponin, 10 m m glycine, and 5% goat serum.

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    Millipore rnaseout
    Rnaseout, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout/product/Millipore
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rnaseout - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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