rnaseout recombinant ribounclease inhibitor  (Thermo Fisher)


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    Name:
    RNaseOUT Recombinant Ribonuclease Inhibitor
    Description:
    RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of 52 kDa RNaseOUT inhibits RNase A RNase B and RNase C ApplicationscDNA synthesis RT PCR and in vitro transcription and translationSourcePurified by affinity chromatography from E coli expressing a cloned porcine genePerformance and quality testingSDS PAGE purity endodeoxyribonuclease assay protein concentration specific activity performance evaluated by RT PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrateUnit reaction conditions100 mM Tris acetate pH 6 5 1 mM EDTA 0 2 mM cCMP 2 µg RNase A in 1 mL from 0 to 10 min at 25°C
    Catalog Number:
    10777019
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher rnaseout recombinant ribounclease inhibitor
    RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of 52 kDa RNaseOUT inhibits RNase A RNase B and RNase C ApplicationscDNA synthesis RT PCR and in vitro transcription and translationSourcePurified by affinity chromatography from E coli expressing a cloned porcine genePerformance and quality testingSDS PAGE purity endodeoxyribonuclease assay protein concentration specific activity performance evaluated by RT PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrateUnit reaction conditions100 mM Tris acetate pH 6 5 1 mM EDTA 0 2 mM cCMP 2 µg RNase A in 1 mL from 0 to 10 min at 25°C
    https://www.bioz.com/result/rnaseout recombinant ribounclease inhibitor/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribounclease inhibitor - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    first-strand systhesis buffer
    dithiothreitol

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    Article Snippet: .. Quantitative PCR The Ambion PureLink RNA mini kit was used to extract total cellular RNA. cDNA reverse transcription was then conducted using Superscript III reverse transcriptase and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). qPCR was performed using BioRad SsoAdvanced SYBR green supermix and the Biorad CFX Connect real-time system. .. Relative gene expression was normalized to the housekeeping gene TBP, and fold-change calculated based on a comparison to the expression level of the adult pancreas.

    In Vitro:

    Article Title: Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers
    Article Snippet: .. In vitro transcription In all, 100 pg to 1000 ng of double-stranded DNA transcription templates was transcribed using 100 U of T7 RNA polymerase (NEB) in 50 µl reactions containing 40 mM Tris–HCl, pH 7.9, 30 mM MgCl2 , 10 mM DTT, 2 mM spermidine, 4 mM ribonucleotide (rNTP) mix and 20 U of the recombinant ribonuclease inhibitor RNaseOUT (Life Technologies). .. Transcription reactions were incubated at 42°C for 1–2 h. After this, transcripts of the circuit components were either (i) used directly for assembly or (ii) subjected to purification before assembly.

    SYBR Green Assay:

    Article Title: Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    Article Snippet: .. Quantitative PCR The Ambion PureLink RNA mini kit was used to extract total cellular RNA. cDNA reverse transcription was then conducted using Superscript III reverse transcriptase and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). qPCR was performed using BioRad SsoAdvanced SYBR green supermix and the Biorad CFX Connect real-time system. .. Relative gene expression was normalized to the housekeeping gene TBP, and fold-change calculated based on a comparison to the expression level of the adult pancreas.

    Incubation:

    Article Title: Replication defective viral genomes exploit a cellular pro-survival mechanism to establish paramyxovirus persistence
    Article Snippet: .. For RNA FISH coupled with immunofluorescence (IF) (RNA FISH-IF), fixed cells were permeabilized with 70% ethanol for 1 h. Permeabilized cells were then incubated with anti-human active caspase-3 antibody (1:100 dilution; Cell Signaling, cat. no. 9661) or anti-human cleaved PARP-1 antibody (1:100 dilution; Cell Signaling, cat. no. 5625) followed by Alexa Fluor 488-labeled goat anti-rabbit IgG (1:500 dilution; Invitrogen, cat. no. R37116) diluted in 1% BSA in the presence of 40 U/ml RNase inhibitor (Invitrogen, cat. no. 10777-019). .. Stained cells were incubated in 4% formaldehyde for 10 min prior to RNA FISH, washed with PBS, and then equilibrated in wash buffer.

    Lysis:

    Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
    Article Snippet: .. Cell pellets from approximately 50 million cells were homogenized, lysed in ice-cold lysis buffer (10 mM HEPES (pH 7.3, ThermoFisher Scientific), 100 mM KCl (1049360250, Merck), 0.5 % NP40 (NP40S, Sigma-Aldrich), 5 mM MgCl2 , 0.5 mM dithiothreitol (DTT, DTT-RO, Sigma-Aldrich), protease inhibitors (118735, Sigma-Aldrich), recombinant RNase inhibitors (10777019, ThermoFisher Schientific), 1 mM PMSF (36978, ThermoFisher Scientific) using TissueLyser LT (50 Hz, 2 min, Qiagen). ..

    Recombinant:

    Article Title: WIN55,212-2 induces cytoplasmic vacuolation in apoptosis-resistant MCL cells
    Article Snippet: .. RNaseOut recombinant ribonuclease inhibitor and the oligo dT primers used were purchased from Invitrogen. .. Quantitative PCR mRNA expression levels of CNR1 and CNR2 were assessed by real-time PCR using Platinum SYBR Green qPCR Supermix-UDG (Invitrogen) according to the manufacturer's protocol.

    Article Title: Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    Article Snippet: .. Quantitative PCR The Ambion PureLink RNA mini kit was used to extract total cellular RNA. cDNA reverse transcription was then conducted using Superscript III reverse transcriptase and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). qPCR was performed using BioRad SsoAdvanced SYBR green supermix and the Biorad CFX Connect real-time system. .. Relative gene expression was normalized to the housekeeping gene TBP, and fold-change calculated based on a comparison to the expression level of the adult pancreas.

    Article Title: Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers
    Article Snippet: .. In vitro transcription In all, 100 pg to 1000 ng of double-stranded DNA transcription templates was transcribed using 100 U of T7 RNA polymerase (NEB) in 50 µl reactions containing 40 mM Tris–HCl, pH 7.9, 30 mM MgCl2 , 10 mM DTT, 2 mM spermidine, 4 mM ribonucleotide (rNTP) mix and 20 U of the recombinant ribonuclease inhibitor RNaseOUT (Life Technologies). .. Transcription reactions were incubated at 42°C for 1–2 h. After this, transcripts of the circuit components were either (i) used directly for assembly or (ii) subjected to purification before assembly.

    Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
    Article Snippet: .. Cell pellets from approximately 50 million cells were homogenized, lysed in ice-cold lysis buffer (10 mM HEPES (pH 7.3, ThermoFisher Scientific), 100 mM KCl (1049360250, Merck), 0.5 % NP40 (NP40S, Sigma-Aldrich), 5 mM MgCl2 , 0.5 mM dithiothreitol (DTT, DTT-RO, Sigma-Aldrich), protease inhibitors (118735, Sigma-Aldrich), recombinant RNase inhibitors (10777019, ThermoFisher Schientific), 1 mM PMSF (36978, ThermoFisher Scientific) using TissueLyser LT (50 Hz, 2 min, Qiagen). ..

    Fluorescence In Situ Hybridization:

    Article Title: Replication defective viral genomes exploit a cellular pro-survival mechanism to establish paramyxovirus persistence
    Article Snippet: .. For RNA FISH coupled with immunofluorescence (IF) (RNA FISH-IF), fixed cells were permeabilized with 70% ethanol for 1 h. Permeabilized cells were then incubated with anti-human active caspase-3 antibody (1:100 dilution; Cell Signaling, cat. no. 9661) or anti-human cleaved PARP-1 antibody (1:100 dilution; Cell Signaling, cat. no. 5625) followed by Alexa Fluor 488-labeled goat anti-rabbit IgG (1:500 dilution; Invitrogen, cat. no. R37116) diluted in 1% BSA in the presence of 40 U/ml RNase inhibitor (Invitrogen, cat. no. 10777-019). .. Stained cells were incubated in 4% formaldehyde for 10 min prior to RNA FISH, washed with PBS, and then equilibrated in wash buffer.

    Immunofluorescence:

    Article Title: Replication defective viral genomes exploit a cellular pro-survival mechanism to establish paramyxovirus persistence
    Article Snippet: .. For RNA FISH coupled with immunofluorescence (IF) (RNA FISH-IF), fixed cells were permeabilized with 70% ethanol for 1 h. Permeabilized cells were then incubated with anti-human active caspase-3 antibody (1:100 dilution; Cell Signaling, cat. no. 9661) or anti-human cleaved PARP-1 antibody (1:100 dilution; Cell Signaling, cat. no. 5625) followed by Alexa Fluor 488-labeled goat anti-rabbit IgG (1:500 dilution; Invitrogen, cat. no. R37116) diluted in 1% BSA in the presence of 40 U/ml RNase inhibitor (Invitrogen, cat. no. 10777-019). .. Stained cells were incubated in 4% formaldehyde for 10 min prior to RNA FISH, washed with PBS, and then equilibrated in wash buffer.

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  • 92
    Thermo Fisher rnaseout recombinant ribonuclease inhibitor
    Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Thermo Fisher
    Average 92 stars, based on 363 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-08
    92/100 stars
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