rnaseout recombinant ribonuclease inhibitor  (Promega)

 
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    Structured Review

    Promega rnaseout recombinant ribonuclease inhibitor
    Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Promega
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-04
    89/100 stars

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    Protease Inhibitor:

    Article Title: P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
    Article Snippet: .. Tandem-affinity purification and mass spectrometry HEK293 cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, and 1 mM dithiothreitol (DTT), supplemented with a protease inhibitor cocktail without EDTA (Roche Diagnostics, Meylan, France), for 30 min on ice, in the presence of either 65 U/ml RNaseOut recombinant ribonuclease inhibitor (Promega) or 20 μg/ml RNase A (Euromedex). .. Cytoplasmic proteins were quantified by the Coomassie protein assay (Thermo Scientific).

    Incubation:

    Article Title: P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
    Article Snippet: Tandem-affinity purification and mass spectrometry HEK293 cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, and 1 mM dithiothreitol (DTT), supplemented with a protease inhibitor cocktail without EDTA (Roche Diagnostics, Meylan, France), for 30 min on ice, in the presence of either 65 U/ml RNaseOut recombinant ribonuclease inhibitor (Promega) or 20 μg/ml RNase A (Euromedex). .. Cleared extracts were then incubated with 120 μl of 50% slurry of the anti-FLAG M2 affinity gel (Sigma-Aldrich) for 2 h at 4°C.

    Recombinant:

    Article Title: P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
    Article Snippet: .. Tandem-affinity purification and mass spectrometry HEK293 cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, and 1 mM dithiothreitol (DTT), supplemented with a protease inhibitor cocktail without EDTA (Roche Diagnostics, Meylan, France), for 30 min on ice, in the presence of either 65 U/ml RNaseOut recombinant ribonuclease inhibitor (Promega) or 20 μg/ml RNase A (Euromedex). .. Cytoplasmic proteins were quantified by the Coomassie protein assay (Thermo Scientific).

    Mass Spectrometry:

    Article Title: P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
    Article Snippet: .. Tandem-affinity purification and mass spectrometry HEK293 cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, and 1 mM dithiothreitol (DTT), supplemented with a protease inhibitor cocktail without EDTA (Roche Diagnostics, Meylan, France), for 30 min on ice, in the presence of either 65 U/ml RNaseOut recombinant ribonuclease inhibitor (Promega) or 20 μg/ml RNase A (Euromedex). .. Cytoplasmic proteins were quantified by the Coomassie protein assay (Thermo Scientific).

    Purification:

    Article Title: P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes
    Article Snippet: .. Tandem-affinity purification and mass spectrometry HEK293 cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, and 1 mM dithiothreitol (DTT), supplemented with a protease inhibitor cocktail without EDTA (Roche Diagnostics, Meylan, France), for 30 min on ice, in the presence of either 65 U/ml RNaseOut recombinant ribonuclease inhibitor (Promega) or 20 μg/ml RNase A (Euromedex). .. Cytoplasmic proteins were quantified by the Coomassie protein assay (Thermo Scientific).

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  • 90
    Promega rnaseout recombinant rnase inhibitor
    Rnaseout Recombinant Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant rnase inhibitor/product/Promega
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant rnase inhibitor - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    89
    Promega rnaseout recombinant ribonuclease inhibitor
    Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Promega
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

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