rnaseout recombinant ribonuclease inhibitor  (Millipore)


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    Structured Review

    Millipore rnaseout recombinant ribonuclease inhibitor
    Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Millipore
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-03
    98/100 stars

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    Related Articles

    Transfection:

    Article Title: A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism
    Article Snippet: .. Transfected HEK293 cells were lysed in polysomal lysis buffer (supplemented with 110 mM potassium acetate, 20 mM magnesium acetate, 10 mM HEPES, 100 mM potassium chloride, 10 mM magnesium chloride, 0.1% NP-40, 2 mM DTT, 40 U/mL RNase inhibitor [Thermo Fisher #10777019), 100 μg/ml cycloheximide [Sigma #C104450] and 1X Protease inhibitor Cocktail V [Calbiochem]). .. Lysates were treated with 1500 U RNase1 (Ambion) for 30 min at 21°C and loaded onto 6 mL polysome lysis buffer layered over 4 mL 32% sucrose cushions.

    Isolation:

    Article Title: Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs
    Article Snippet: Lysates were prepared with 1mL of polysome lysis buffer (15 mM Tris pH 7.4, 250 mM NaCl, 15 mM MgCl2, 1% Triton X-100, 100 g/ml cyclohexamide, 1 mM DTT, 400 U/ml RNase inhibitors (Invitrogen cat# 10777019) and protease inhibitors (Sigma Aldrich cat # 111697498001). .. RNAs were isolated from polysomal fractions using TRIzol reagent.

    Protease Inhibitor:

    Article Title: A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism
    Article Snippet: .. Transfected HEK293 cells were lysed in polysomal lysis buffer (supplemented with 110 mM potassium acetate, 20 mM magnesium acetate, 10 mM HEPES, 100 mM potassium chloride, 10 mM magnesium chloride, 0.1% NP-40, 2 mM DTT, 40 U/mL RNase inhibitor [Thermo Fisher #10777019), 100 μg/ml cycloheximide [Sigma #C104450] and 1X Protease inhibitor Cocktail V [Calbiochem]). .. Lysates were treated with 1500 U RNase1 (Ambion) for 30 min at 21°C and loaded onto 6 mL polysome lysis buffer layered over 4 mL 32% sucrose cushions.

    Spectrophotometry:

    Article Title: Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs
    Article Snippet: Lysates were prepared with 1mL of polysome lysis buffer (15 mM Tris pH 7.4, 250 mM NaCl, 15 mM MgCl2, 1% Triton X-100, 100 g/ml cyclohexamide, 1 mM DTT, 400 U/ml RNase inhibitors (Invitrogen cat# 10777019) and protease inhibitors (Sigma Aldrich cat # 111697498001). .. Polysome fractions were collected by continuously monitoring and recording the A254 on a Gradient Station IP (Biocomp) attached to a UV-MII (GE Healthcare, CA) spectrophotometer.

    Lysis:

    Article Title: A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism
    Article Snippet: .. Transfected HEK293 cells were lysed in polysomal lysis buffer (supplemented with 110 mM potassium acetate, 20 mM magnesium acetate, 10 mM HEPES, 100 mM potassium chloride, 10 mM magnesium chloride, 0.1% NP-40, 2 mM DTT, 40 U/mL RNase inhibitor [Thermo Fisher #10777019), 100 μg/ml cycloheximide [Sigma #C104450] and 1X Protease inhibitor Cocktail V [Calbiochem]). .. Lysates were treated with 1500 U RNase1 (Ambion) for 30 min at 21°C and loaded onto 6 mL polysome lysis buffer layered over 4 mL 32% sucrose cushions.

    Article Title: Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs
    Article Snippet: .. Lysates were prepared with 1mL of polysome lysis buffer (15 mM Tris pH 7.4, 250 mM NaCl, 15 mM MgCl2, 1% Triton X-100, 100 g/ml cyclohexamide, 1 mM DTT, 400 U/ml RNase inhibitors (Invitrogen cat# 10777019) and protease inhibitors (Sigma Aldrich cat # 111697498001). .. Equal amounts (5–10 mg) of protein lysates were layered on a 20%–50% linear sucrose gradient (20% and 50% sucrose solutions in 15 mM Tris pH 7.4, 15 mM MgCl2, 150 mM NaCl, 1 mM DTT, 100 μg/Ml cyclohexamide and 20 U/ml RNase inhibitors), mixed on Gradient Station IP Biocomp and centrifuged in a Beckman SW41Ti rotor at 92,000 g for 3 hr at 4°C.

    Western Blot:

    Article Title: A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism
    Article Snippet: Transfected HEK293 cells were lysed in polysomal lysis buffer (supplemented with 110 mM potassium acetate, 20 mM magnesium acetate, 10 mM HEPES, 100 mM potassium chloride, 10 mM magnesium chloride, 0.1% NP-40, 2 mM DTT, 40 U/mL RNase inhibitor [Thermo Fisher #10777019), 100 μg/ml cycloheximide [Sigma #C104450] and 1X Protease inhibitor Cocktail V [Calbiochem]). .. The supernatants were removed and pellets were dissolved in 6× SDS loading buffer for western blot analysis.

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