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Fisher Scientific rnaseout recombinant ribonuclease inhibitor
Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Fisher Scientific
Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Electron Microscopy:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Amplification:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Magnetic Beads:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Isolation:

Article Title: DIE-RNA: A Reproducible Strategy for the Digestion of Normal and Injured Pancreas, Isolation of Pancreatic Cells from Genetically Engineered Mouse Models and Extraction of High Quality RNA
Article Snippet: .. - Agilent RNA 6000 Nano kit (5067-1512, Agilent Genomics, USA) - Bovine serum albumin (BSA) (160069 MP, Merck, Belgium) - Caerulein (C9026, Merck, Belgium) - Collagenase P 500 mg (1121386500, Merck, Belgium) - Deoxyribonucleotide (dNTP) (03622614001, Merck, Belgium) - Dulbecco's phosphate-buffered saline (DPBS) (BE17-512F, Lonza, Belgium) - High-capacity complementary deoxyribonucleic acid (cDNA) reverse transcription kit (4368814, Life technology Europe, Belgium) - KAPA SYBR FAST (KK4608, Merck, Belgium) - Nuclease-Free water (Promega, Netherlands) - Prepared solutions listed in Table - Pure Ethanol 100% (UN1170, VWR Chemicals, Belgium) - RNAqueous-Micro total RNA isolation kit (AM1931, Fisher Scientific, Belgium) - RNaseOut (10777019, Life technology Europe, Belgium) - Tamoxifen (T5648, Merck, Belgium) - Trypan blue (T8154, Merck, Belgium) .. - 0.22 μm-filter (430758, Fisher Scientific, Belgium) - 30-gauge needle (30400, BD Biosciences, Belgium) - 40 μm-cell strainer (431750, Fisher Scientific, Belgium) - 0.2, 0.5, and 1.5 mL-Sterile Eppendorf tubes (Eppendorf, Belgium) - 15 and 50 mL-Polypropylene Falcon tubes (Fisher Scientific, Belgium) - 250 and 500 mL-Pyrex bottles (Fisher Scientific, Belgium) - 96-well PCR plates (HSP9655, Bio-Rad, Belgium) - Automated cell counter (TC20, Bio-Rad, Belgium) - Cell sorter machine with water bath (FACS ARIAIII, BD Biosciences, Belgium) - CFX96 real-time PCR machine (C1000, Bio-Rad, Belgium) - Fluorescence microscope (Axiovert 200, Zeiss, Belgium) - Incubator at 37°C with a rotating plate (Eppendorf, Belgium) - Magnetic bar and agitator - Nanodrop spectrophotometer (ND-1000, Isogen, Belgium) - Nuclease-free tips and pipettes (Westburg, Netherlands) - Optical microscope (Axiovert 40C, Zeiss, Belgium) - Pyrex beaker (Fisher Scientific, Belgium) - RNA BioAnalyzer system (Agilent 2100, Agilent Genomics, Belgium) - Sterile dissection tools (i.e., scissors, forceps, and clamps) (Fine Science Tools, Germany) - Sterile Petri dishes (Fisher Scientific, Belgium) - Thermo-cycler machine (T100, Bio-Rad, Belgium)

Article Title: DIE-RNA: A Reproducible Strategy for the Digestion of Normal and Injured Pancreas, Isolation of Pancreatic Cells from Genetically Engineered Mouse Models and Extraction of High Quality RNA
Article Snippet: .. Reagents and enzymes - Agilent RNA 6000 Nano kit (5067-1512, Agilent Genomics, USA) - Bovine serum albumin (BSA) (160069 MP, Merck, Belgium) - Caerulein (C9026, Merck, Belgium) - Collagenase P 500 mg (1121386500, Merck, Belgium) - Deoxyribonucleotide (dNTP) (03622614001, Merck, Belgium) - Dulbecco's phosphate-buffered saline (DPBS) (BE17-512F, Lonza, Belgium) - High-capacity complementary deoxyribonucleic acid (cDNA) reverse transcription kit (4368814, Life technology Europe, Belgium) - KAPA SYBR FAST (KK4608, Merck, Belgium) - Nuclease-Free water (Promega, Netherlands) - Prepared solutions listed in Table - Pure Ethanol 100% (UN1170, VWR Chemicals, Belgium) - RNAqueous-Micro total RNA isolation kit (AM1931, Fisher Scientific, Belgium) - RNaseOut (10777019, Life technology Europe, Belgium) - Tamoxifen (T5648, Merck, Belgium) - Trypan blue (T8154, Merck, Belgium) .. Equipment - 0.22 μm-filter (430758, Fisher Scientific, Belgium) - 30-gauge needle (30400, BD Biosciences, Belgium) - 40 μm-cell strainer (431750, Fisher Scientific, Belgium) - 0.2, 0.5, and 1.5 mL-Sterile Eppendorf tubes (Eppendorf, Belgium) - 15 and 50 mL-Polypropylene Falcon tubes (Fisher Scientific, Belgium) - 250 and 500 mL-Pyrex bottles (Fisher Scientific, Belgium) - 96-well PCR plates (HSP9655, Bio-Rad, Belgium) - Automated cell counter (TC20, Bio-Rad, Belgium) - Cell sorter machine with water bath (FACS ARIAIII, BD Biosciences, Belgium) - CFX96 real-time PCR machine (C1000, Bio-Rad, Belgium) - Fluorescence microscope (Axiovert 200, Zeiss, Belgium) - Incubator at 37°C with a rotating plate (Eppendorf, Belgium) - Magnetic bar and agitator - Nanodrop spectrophotometer (ND-1000, Isogen, Belgium) - Nuclease-free tips and pipettes (Westburg, Netherlands) - Optical microscope (Axiovert 40C, Zeiss, Belgium) - Pyrex beaker (Fisher Scientific, Belgium) - RNA BioAnalyzer system (Agilent 2100, Agilent Genomics, Belgium) - Sterile dissection tools (i.e., scissors, forceps, and clamps) (Fine Science Tools, Germany) - Sterile Petri dishes (Fisher Scientific, Belgium) - Thermo-cycler machine (T100, Bio-Rad, Belgium)

Dissection:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

SYBR Green Assay:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Mass Spectrometry:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Polymerase Chain Reaction:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Recombinant:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation). .. MiSeq v2 300 cycle kit (Illumina, cat no. MS-102–2002) NextSeq 500/550 High Output v2 75 cycle kit (Illumina, cat. no. FC-404–2005) PhiX Control v3 (Illumina, FC-110–3001)

Plasmid Preparation:

Article Title: Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT
Article Snippet: Plasmid DNA Mini Kit I (Omega, cat. no. D6942–02) or equivalent E.Z.N.A. .. Total RNA Kit I (Omega, cat. no. R6834–02) or equivalent RNA Clean & Concetrator-25 (Zymo Research, cat. no. R1018) mMESSAGE mMACHINE SP6 (Thermo Fisher Scientific, AM1340) NaOH, 1M (Honeywell Research Chemicals, cat. no. 319511–1L) Tris-HCl pH 7.5, 1M (VWR, cat. no. 75800–958) Tris–HCl pH 7.0, 1 M (Thermo Fisher Scientific, cat. no. AM9851) Tris–HCl pH 8.0, (Thermo Fisher Scientific, cat. no. 15568025) EnGen sgRNA Synthesis Kit, S. pyogenes (NEB, cat. no. E3322) CRITICAL sgRNA oligonucleotide sequences (sgRNA 1–4) provided (see below) are designed for use with this kit EnGen Cas9 NLS, S. pyogenes (NEB, cat. no. M0646) iTaq Universal SYBR Green Supermix (Biorad, cat. no. 1725120) Phusion High-Fidelity DNA polymerase (NEB, cat. no. M0530) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme Q5 High-Fidelity DNA polymerase (NEB, cat. no. M0491) CRITICAL Amplification of the scGESTALT library has been optimized using this enzyme KAPA 2× HiFi HotStart PCR mix (KAPABiosystems, cat. no. KK2601) dNTP mix, 10 mM each (NEB, cat. no. N0447) 100 bp DNA ladder (NEB, cat. no. N3231) Papain Dissociation System (Worthington, cat. no. ) CRITICAL Brain dissociation has been optimized using this kit Neurobasal media (Thermo Fisher Scientific, cat. no. 21103049) CRITICAL Brain dissection and dissociation have been optimized using this media B27 supplement, serum free, 50× (Thermo Fisher Scientific, cat. no. 17504044) CRITICAL Brain dissection has been optimized using this supplement for high cell viability DPBS, no calcium, no magnesium (Thermo Fisher Scientific, cat. no. 14190144) DBPS, calcium, magnesium, glucose, pyruvate (Thermo Fisher Scientific, cat no. 14287080) 35 μm cell strainer (e.g. VWR, cat. no. 352235) 20 μm cell strainer (e.g. Sysmex, cat. no. 04–004-2325) Trypan blue, 0.4% (wt/vol) (Thermo Fisher Scientific, cat. no. ) OptiPrep Density Gradient Medium (Sigma-Aldrich, cat. no. D1556–250ML) Agencourt AMPure XP magnetic beads (Beckman Coulter, cat. no. ) Random Hexamers, 50 μM (Fisher Scientific, cat. no. N8080127) PrimeScript Reverse Transcriptase (Takara Clonetec, cat. no. 2680A) RNaseOUT Recombinant Ribonuclease Inhibitor (Fisher Scientific, cat. no. 10777019) Phenol red, 0.5% (wt/vol) (Sigma-Aldrich, cat. no. P0290) Methylene blue, 0.1% (wt/vol) (Aqua Solutions, cat. no. 5761–500ML) Instant Ocean salt (Instant Ocean) Agarose (e.g. National Diagnostics, cat. no. EC-202) Sylgard 184 (Electron Microscopy Sciences, cat. no. 24236–10) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. ) Tricaine (Sigma-Aldrich, cat. no. –50G) CAUTION: Use gloves when handling (can cause skin irritation).

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    Fisher Scientific rnaseout recombinant ribonuclease inhibitor
    Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Fisher Scientific
    Average 94 stars, based on 2 article reviews
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    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-04
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