rnase free dnasei  (Qiagen)


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    Structured Review

    Qiagen rnase free dnasei
    Rnase Free Dnasei, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnasei/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase free dnasei - by Bioz Stars, 2021-04
    86/100 stars

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    Incubation:

    Article Title: The EP4 receptor antagonist, L-161,982, blocks prostaglandin E2-induced Signal Transduction and Cell Proliferation in HCA-7 colon cancer cells
    Article Snippet: Total RNA from HCA-7 cells was isolated using RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. To avoid genomic DNA contamination, samples were incubated with RNase-free DNaseI (Qiagen) during RNA preparation. .. Two micrograms of total RNA was reverse transcribed using iScript cDNA synthesis kit (BioRad, Hercules, CA).

    Purification:

    Article Title: Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi
    Article Snippet: For quantification of ospC transcripts of B. burgdorferi in ticks, RNA samples were extracted from ticks using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer's protocols. .. To reduce trace amounts of DNA contamination, samples were further digested with RNase-free DNaseI (Qiagen), purified using the RNeasy mini kit (Qiagen), and analyzed with NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific). .. DNA-free RNA was confirmed by PCR amplification for the B. burgdorferi flaB gene. cDNA was synthesized using the PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa).

    Article Title: The Oligopeptide ABC transporter OppA4 negatively regulates the virulence factor OspC production of the Lyme disease pathogen
    Article Snippet: Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. To reduce trace amounts of DNA contamination, samples were further digested with RNase-free DNaseI (Qiagen), and purified using the RNeasy kit (Qiagen). .. DNA-free RNA was confirmed by PCR. cDNA was generated using the SuperScript III First-Strand Synthesis System according to the manufacturer’s protocol (Invitrogen).

    Article Title: Two Distinct Forms of M-Locus Protein Kinase Localize to the Plasma Membrane and Interact Directly with S-Locus Receptor Kinase to Transduce Self-Incompatibility Signaling in Brassica rapa [W]
    Article Snippet: Two, one, and seven positive clones were obtained from 100,000 clones of the S 36 stigma cDNA library, 40,000 clones of the S 9 stigma cDNA library, and 200,000 clones of the S 9 stigma full-length cDNA library, respectively. .. Real-Time RT-PCR Total RNA samples from B. rapa tissues were purified using ISOGEN (Nippongene) according to the manufacturer's protocols and treated with RNase-free DNaseI (Qiagen). .. The concentration and purity of total plant RNA was determined by spectrophotometric analysis.

    Spectrophotometry:

    Article Title: Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi
    Article Snippet: For quantification of ospC transcripts of B. burgdorferi in ticks, RNA samples were extracted from ticks using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer's protocols. .. To reduce trace amounts of DNA contamination, samples were further digested with RNase-free DNaseI (Qiagen), purified using the RNeasy mini kit (Qiagen), and analyzed with NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific). .. DNA-free RNA was confirmed by PCR amplification for the B. burgdorferi flaB gene. cDNA was synthesized using the PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa).

    RNA Sequencing Assay:

    Article Title: The Mitochondrial Protease LonP1 Promotes Proteasome Inhibitor Resistance in Multiple Myeloma
    Article Snippet: Mitochondrial protease expression levels in MM.1S myeloma cell lines following treatment with 6 µM lactacystin or 60 nM bortezomib for six hours were compared to mock-treated cells (DMSO at 0.1% v /v). .. RNA was extracted with the RNeasy mini kit with RNAse-free DNaseI on column treatment (Qiagen, Hilden, Germany, #74134 & #79254) and quantified by paired-end RNA-sequencing on an Illumina HiSeq2500, as previously published [ ]. .. RNA-seq datasets have been deposited with the Gene Expression Omnibus (GEO accession number GSE166122).

    Isolation:

    Article Title: The identification of Hoxc8 target genes
    Article Snippet: RNA Preparation, Real-Time PCR Quantification, and Semiquantitative RT-PCR. .. Total RNA was isolated with RNeasy miniprep columns and an RNase-free DNaseI kit (Qiagen, Valencia, CA). .. The cDNA for real-time PCR and semiquantitative RT-PCR was made by using the OmniScript kit (Qiagen).

    Quantitative RT-PCR:

    Article Title: Two Distinct Forms of M-Locus Protein Kinase Localize to the Plasma Membrane and Interact Directly with S-Locus Receptor Kinase to Transduce Self-Incompatibility Signaling in Brassica rapa [W]
    Article Snippet: Two, one, and seven positive clones were obtained from 100,000 clones of the S 36 stigma cDNA library, 40,000 clones of the S 9 stigma cDNA library, and 200,000 clones of the S 9 stigma full-length cDNA library, respectively. .. Real-Time RT-PCR Total RNA samples from B. rapa tissues were purified using ISOGEN (Nippongene) according to the manufacturer's protocols and treated with RNase-free DNaseI (Qiagen). .. The concentration and purity of total plant RNA was determined by spectrophotometric analysis.

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  • 86
    Qiagen rnase free dnase set
    Rnase Free Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase set/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase set - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Qiagen rnase free dnasei
    Rnase Free Dnasei, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnasei/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase free dnasei - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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