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GE Healthcare rnaguard
Rnaguard, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaguard/product/GE Healthcare
Average 90 stars, based on 9 article reviews
Price from $9.99 to $1999.99
rnaguard - by Bioz Stars, 2020-05
90/100 stars

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Lysis:

Article Title: In Situ GABAergic Modulation of Synchronous Gonadotropin Releasing Hormone-1 Neuronal Activity
Article Snippet: .. Briefly, 1 μg of brain RNA or a single cell was placed into a reaction mixture containing 4 μl of lysis buffer [for 100 μl of mix on ice, 20 μl of Moloney murine leukemia virus (MMLV) buffer 5× [Bethesda Research Labs (BRL), Branchburg, NJ], 76 μl of H2 O, 0.5 μl of NP40 (Amersham Biosciences, Piscataway, NJ), 1 μl of PrimeRNase inhibitor (3′5′ Inc., Boulder, CO), 1 μl of RNAguard (Amersham), 2 μl of a freshly made 1:24 dilution of the stock primer mix [10 μl of each 100 m m dNTP (BRL), 10 μl of 50 OD/ml pd(T) 19–24, and 30 μl of H2 O]]. ..

Incubation:

Article Title: Role of cis-acting elements in the control of SERCA2b Ca2+ pump mRNA decay by nuclear proteins
Article Snippet: .. Typically, 5 ng of 33 P-labelled RNA was incubated with 25 μg of the protein extracts for 0–30 min at 37 °C in a reaction buffer containing 10 mM Mops/NaOH (pH 7.2), 200 mM NaCl, 2.5 mM magnesium acetate, 1 mM ATP, 0.1 mM spermine, 2 mM dithiothreitol and 1 unit/μl RNAguard (Amersham Biosciences). .. Intensity of the bands was determined using the ImageQuant software, and the mean of three background intensities obtained from the same gel was subtracted.

Article Title: Characterization of the Mechanisms Controlling Greatwall Activity ▿
Article Snippet: .. For mRNA translation, CSF egg extracts were supplemented with dithiothreitol (DTT; 1 mM), RNAguard (0.4 U/μl extract; Amersham Biosciences), tRNA (0.1 μg/μl), and the corresponding mRNA (0.05 μg/μl extract) and incubated for 2 h at 20°C ( ). .. Immunoprecipitations/immunodepletions were performed using 10 μl of extracts, 10 μl of magnetic protein G-Dynabeads (Dynal), and 2 μg of each antibody.

Article Title: Prematurely Elevating Estradiol in Early Baboon Pregnancy Suppresses Uterine Artery Remodeling and Expression of Extravillous Placental Vascular Endothelial Growth Factor and ?1?1 and ?5?1 Integrins
Article Snippet: .. Supplied reaction buffer, 0.1 m dithiothreitol, 200 U of Superscript III RT (Invitrogen), and 40 U of RNAguard (Amersham Pharmacia Biotech, Piscataway, NJ) were then added, and the entire mixture was incubated at 25 C for 5 min and 50 C for 60 min. .. The RT reaction was terminated by heat inactivation at 70 C for 15 min and cooled to 4 C. mRNA levels were quantified via a LightCycler real-time PCR unit (Roche Diagnostics Corp.) using Fast Start DNA Master SYBR Green I kit for PCR.

other:

Article Title: Phylogenetic analysis of the non-structural (NS) gene of influenza A viruses isolated from mallards in Northern Europe in 2005
Article Snippet: The RT mix comprised 2.5 μl of DMPC water, 5 μl of 5× First Strand buffer (Invitrogen), 0.5 μl of 10 mM dNTP mix (Amersham Biosciences), 2 μl of 50 mM random primers (pdN6), 32 U of RNAguard (Amersham Biosciences), 200 U of MMLV reverse transcriptase (Invitrogen) and 5 μl RNA solution in total volume of 25 μl.

Article Title: Mitochondria maintain maturation and secretion of lipoprotein lipase in the endoplasmic reticulum
Article Snippet: RNAguard and random hexameric primers were purchased from Amersham Biosciences.

Random Hexamer Labeling:

Article Title: Fast erg K+ currents in rat embryonic serotonergic neurones
Article Snippet: .. Reverse transcription was carried out in a volume of 10 μl adding following substances to the indicated final concentrations: 2 m m Tris/HCl (pH 8.0), 10 m m DTT, 0.5 m m of each dNTP, 5 μ m pd(N6) random hexamer 5′-phosphate (Amersham Pharmacia Biotech, Freiburg, Germany), 20 U RNAguard (Amersham), 100 U M-MLV reverse transcriptase (Invitrogen). ..

Purification:

Article Title: Silencing of Amyloid Precursor Protein Expression Using a New Engineered Delta Ribozyme
Article Snippet: .. In the case of the SOFA-HDV ribozymes, the resuspended DNA pellets were used in 100-μ L transcription reactions containing 80 mM HEPES-KOH (pH 7.5), 24 mM MgCl2 , 2 mM spermidine, 40 mM DTT, 5 mM of each rNTP, 0.01 U of pyrophosphatase (Roche Diagnostics), 24 U of RNAGuard (Amersham Biosciences), and 10 μ g of purified T7 RNA polymerase and allowed to proceed for 4 h at 37°C. .. The reactions were then treated with 4 U of RQ1 DNase (Promega), phenol-chloroform extracted, ethanol precipitated, and washed.

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    GE Healthcare rnaguard
    Rnaguard, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaguard/product/GE Healthcare
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rnaguard - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    88
    GE Healthcare rnaguard rnase inhibitor
    Rnaguard Rnase Inhibitor, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaguard rnase inhibitor/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnaguard rnase inhibitor - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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