rnaase inhibitor superase  (Thermo Fisher)


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  • 93

    Structured Review

    Thermo Fisher rnaase inhibitor superase
    Rnaase Inhibitor Superase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaase inhibitor superase/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnaase inhibitor superase - by Bioz Stars, 2020-09
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: An ARF6-Exportin-5 Axis Delivers pre-miRNA Cargo to Tumor Microvesicles.
    Article Snippet: .. Isolated TMVs were resuspended in sterile filtered PBS and incubated with 2 mg/mL RNase A (Qiagen) for 30 minutes at 37˚C in prior to being re-isolated and lysed in RIP buffer consisting of 150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% Triton X-100 with protease inhibitor cocktail and 100 U/mL RNAase inhibitor SUPERase (Life Technologies) added immediately prior to use. .. The insoluble fraction was removed by centrifugation at 18,000 g for 15 minutes and the supernatant transferred to a new tube.

    Incubation:

    Article Title: An ARF6-Exportin-5 Axis Delivers pre-miRNA Cargo to Tumor Microvesicles.
    Article Snippet: .. Isolated TMVs were resuspended in sterile filtered PBS and incubated with 2 mg/mL RNase A (Qiagen) for 30 minutes at 37˚C in prior to being re-isolated and lysed in RIP buffer consisting of 150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% Triton X-100 with protease inhibitor cocktail and 100 U/mL RNAase inhibitor SUPERase (Life Technologies) added immediately prior to use. .. The insoluble fraction was removed by centrifugation at 18,000 g for 15 minutes and the supernatant transferred to a new tube.

    Protease Inhibitor:

    Article Title: An ARF6-Exportin-5 Axis Delivers pre-miRNA Cargo to Tumor Microvesicles.
    Article Snippet: .. Isolated TMVs were resuspended in sterile filtered PBS and incubated with 2 mg/mL RNase A (Qiagen) for 30 minutes at 37˚C in prior to being re-isolated and lysed in RIP buffer consisting of 150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% Triton X-100 with protease inhibitor cocktail and 100 U/mL RNAase inhibitor SUPERase (Life Technologies) added immediately prior to use. .. The insoluble fraction was removed by centrifugation at 18,000 g for 15 minutes and the supernatant transferred to a new tube.