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TaKaRa rnaase free dnaase i
Rnaase Free Dnaase I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaase free dnaase i/product/TaKaRa
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
rnaase free dnaase i - by Bioz Stars, 2020-04
91/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
Article Snippet: .. Quantitative PCR RNA was isolated from approximately 200 dissected antennae per genotype using the easy-BLUE kit (Intron, South Korea) and treated with RNAase-free DNAase I (Takara, Japan). .. Antennal cDNAs were synthesized adding 2 μ g of total antennal RNAs to the SuperScript III First-strand Synthesis System (Invitrogen, USA).

Article Title: Experimental Verification of a Predicted Intronic MicroRNA in Human NGFR Gene with a Potential Pro-Apoptotic Function
Article Snippet: Residual DNA was removed using RNAase-free DNAase I (Takara) at 37°C for 30 min followed by heat inactivation at 65°C for 10 min by addition of EDTA. .. Real-time PCR detection of precursor and mir-6165 mature form.

RNA Extraction:

Article Title: Epigenetically downregulated Semaphorin 3E contributes to gastric cancer
Article Snippet: Paragraph title: RNA extraction ... RNAase-free DNAase I (Takara, Japan) was used to remove possible genomic DNA contamination.

Article Title: Experimental Verification of a Predicted Intronic MicroRNA in Human NGFR Gene with a Potential Pro-Apoptotic Function
Article Snippet: Paragraph title: RNA Extraction ... Residual DNA was removed using RNAase-free DNAase I (Takara) at 37°C for 30 min followed by heat inactivation at 65°C for 10 min by addition of EDTA.

Sequencing:

Article Title: An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
Article Snippet: Quantitative PCR RNA was isolated from approximately 200 dissected antennae per genotype using the easy-BLUE kit (Intron, South Korea) and treated with RNAase-free DNAase I (Takara, Japan). .. Quantitative PCR was performed using the SYBR Green reagent on a StepOne Plus Sequence detection system (Applied Biosystems, USA).

Synthesized:

Article Title: An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
Article Snippet: Quantitative PCR RNA was isolated from approximately 200 dissected antennae per genotype using the easy-BLUE kit (Intron, South Korea) and treated with RNAase-free DNAase I (Takara, Japan). .. Antennal cDNAs were synthesized adding 2 μ g of total antennal RNAs to the SuperScript III First-strand Synthesis System (Invitrogen, USA).

Isolation:

Article Title: An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
Article Snippet: .. Quantitative PCR RNA was isolated from approximately 200 dissected antennae per genotype using the easy-BLUE kit (Intron, South Korea) and treated with RNAase-free DNAase I (Takara, Japan). .. Antennal cDNAs were synthesized adding 2 μ g of total antennal RNAs to the SuperScript III First-strand Synthesis System (Invitrogen, USA).

Spectrophotometry:

Article Title: Epigenetically downregulated Semaphorin 3E contributes to gastric cancer
Article Snippet: RNAase-free DNAase I (Takara, Japan) was used to remove possible genomic DNA contamination. .. The concentration and quality of the total RNA were assessed with Nanodrop Spectrophotometer (NanoDrop 2000c, Thermo Fisher Scientific, USA).

SYBR Green Assay:

Article Title: An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
Article Snippet: Quantitative PCR RNA was isolated from approximately 200 dissected antennae per genotype using the easy-BLUE kit (Intron, South Korea) and treated with RNAase-free DNAase I (Takara, Japan). .. Quantitative PCR was performed using the SYBR Green reagent on a StepOne Plus Sequence detection system (Applied Biosystems, USA).

Concentration Assay:

Article Title: Epigenetically downregulated Semaphorin 3E contributes to gastric cancer
Article Snippet: RNAase-free DNAase I (Takara, Japan) was used to remove possible genomic DNA contamination. .. The concentration and quality of the total RNA were assessed with Nanodrop Spectrophotometer (NanoDrop 2000c, Thermo Fisher Scientific, USA).

Incubation:

Article Title: Experimental Verification of a Predicted Intronic MicroRNA in Human NGFR Gene with a Potential Pro-Apoptotic Function
Article Snippet: Residual DNA was removed using RNAase-free DNAase I (Takara) at 37°C for 30 min followed by heat inactivation at 65°C for 10 min by addition of EDTA. .. Briefly, 1 ug of total RNA sample was used in 20 ul Poly A tailing reaction containing; 2.5 U PolyA polymerase (BioLab), 2 ul of 10 mMol ATP and incubated at 37°C for 10 min. Then, the total poly adenylated RNA was used in 10 µL first strand cDNA synthesis reaction using PrimeScript II reverse transcriptase (Takara) and a cocktail of specific oligo-dT primers containing a universal anchor ( ). cDNA synthesis reaction was performed at 42°C for 30 min and terminated at 80°C for 5 s. Real-time quantitative PCR was performed using standard protocols on an ABI PRISM 7500 instrument (Applied Biosystems).

Polymerase Chain Reaction:

Article Title: Experimental Verification of a Predicted Intronic MicroRNA in Human NGFR Gene with a Potential Pro-Apoptotic Function
Article Snippet: Residual DNA was removed using RNAase-free DNAase I (Takara) at 37°C for 30 min followed by heat inactivation at 65°C for 10 min by addition of EDTA. .. According to the predicted precursor and mature miRNA sequences, primers were designed for quantitative PCR , using NCBI primer-blast, MWG Operon online PCR primer design tool ( www.eurofinsdna.com ) and primer bank ( http://pga.mgh.harvard.edu/primerbank/ ).

T-Test:

Article Title: An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
Article Snippet: Quantitative PCR RNA was isolated from approximately 200 dissected antennae per genotype using the easy-BLUE kit (Intron, South Korea) and treated with RNAase-free DNAase I (Takara, Japan). .. Relative concentrations were calculated with the 2−ΔΔ Ct method using rp49 as a control and statistical significance was determined using a t-test.

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    TaKaRa rnaase free dnaase i
    Rnaase Free Dnaase I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaase free dnaase i/product/TaKaRa
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rnaase free dnaase i - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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