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Promega rnaase free dnaase i
Rnaase Free Dnaase I, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaase free dnaase i/product/Promega
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rnaase free dnaase i - by Bioz Stars, 2020-04
86/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Transcriptome profiling of drought responsive noncoding RNAs and their target genes in rice
Article Snippet: Thermocycling and fluorescence detection were performed uisng a Strategene Mx300p real-time PCR machine and Mx3000p software version 2.02 (Stratagene). .. Two hundred ng of total RNA was treated with RNAase-free DNase I (Promega), and transcribed into cDNA using gene specific RT primers and a thermostable reverse transcriptase (Invitrogen).

Article Title: Low-dose paclitaxel ameliorates fibrosis in the remnant kidney model by down-regulating miR-192
Article Snippet: Paragraph title: Real-time polymerase chain reaction ... Contaminated DNA was removed by treating the samples with RNAase-free DNAase I (Promega, Madison, WI, USA).

Amplification:

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA. .. Lack of amplification of 18S rRNA was used to check the successful removal of DNA.

Fluorescence:

Article Title: Transcriptome profiling of drought responsive noncoding RNAs and their target genes in rice
Article Snippet: Thermocycling and fluorescence detection were performed uisng a Strategene Mx300p real-time PCR machine and Mx3000p software version 2.02 (Stratagene). .. Two hundred ng of total RNA was treated with RNAase-free DNase I (Promega), and transcribed into cDNA using gene specific RT primers and a thermostable reverse transcriptase (Invitrogen).

Isolation:

Article Title: The MYST Family Histone Acetyltransferase Regulates Gene Expression and Cell Cycle in Malaria Parasite Plasmodium falciparum
Article Snippet: Paragraph title: RNA isolation, Northern blot, real-time RT-PCR and RACE ... The RNA was treated with RNAase-free DNase I (Promega, Madison, WI) to remove contaminating genomic DNA.

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: Total RNA was isolated from the different tissues using TRI Reagent® RNA/DNA/Protein Isolation Reagent (Molecular Research Center, Cincinnati, OH, USA) following the manufacturer's instructions. .. Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA.

Article Title: Low-dose paclitaxel ameliorates fibrosis in the remnant kidney model by down-regulating miR-192
Article Snippet: Real-time polymerase chain reaction Total RNA was isolated using the High Pure RNA Isolation Kit (Roche, Switzerland) according to the manufacturer's instructions. .. Contaminated DNA was removed by treating the samples with RNAase-free DNAase I (Promega, Madison, WI, USA).

Northern Blot:

Article Title: The MYST Family Histone Acetyltransferase Regulates Gene Expression and Cell Cycle in Malaria Parasite Plasmodium falciparum
Article Snippet: Paragraph title: RNA isolation, Northern blot, real-time RT-PCR and RACE ... The RNA was treated with RNAase-free DNase I (Promega, Madison, WI) to remove contaminating genomic DNA.

Incubation:

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: .. Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA. .. Lack of amplification of 18S rRNA was used to check the successful removal of DNA.

Labeling:

Article Title: The MYST Family Histone Acetyltransferase Regulates Gene Expression and Cell Cycle in Malaria Parasite Plasmodium falciparum
Article Snippet: The RNA was treated with RNAase-free DNase I (Promega, Madison, WI) to remove contaminating genomic DNA. .. A 360 bp PCR product of Pfmyst F2 × Pfmyst R3 ( ) was labeled using the DIG PCR labeling kit (Roche Applied Science, Indianapolis, IN).

SYBR Green Assay:

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA. .. Expression analysis was performed by quantitative RT–PCR in an iCycler iQ System (Bio-Rad, Hercules, CA, USA) using iQ SYBR-Green Supermix (Bio-Rad) and gene-specific primers ( ).

Article Title: Low-dose paclitaxel ameliorates fibrosis in the remnant kidney model by down-regulating miR-192
Article Snippet: Contaminated DNA was removed by treating the samples with RNAase-free DNAase I (Promega, Madison, WI, USA). .. Real-time PCR was performed using a Bio-Rad (Hercules, CA) IQ SYBR Green Supermix with Opticon (MJ Research Inc., Waltham, MA, USA).

Polymerase Chain Reaction:

Article Title: The MYST Family Histone Acetyltransferase Regulates Gene Expression and Cell Cycle in Malaria Parasite Plasmodium falciparum
Article Snippet: The RNA was treated with RNAase-free DNase I (Promega, Madison, WI) to remove contaminating genomic DNA. .. A 360 bp PCR product of Pfmyst F2 × Pfmyst R3 ( ) was labeled using the DIG PCR labeling kit (Roche Applied Science, Indianapolis, IN).

Generated:

Article Title: Low-dose paclitaxel ameliorates fibrosis in the remnant kidney model by down-regulating miR-192
Article Snippet: Contaminated DNA was removed by treating the samples with RNAase-free DNAase I (Promega, Madison, WI, USA). .. First-strand cDNAs were generated using a superscript VILO cDNA synthesis kit (Invitrogen).

Quantitative RT-PCR:

Article Title: The MYST Family Histone Acetyltransferase Regulates Gene Expression and Cell Cycle in Malaria Parasite Plasmodium falciparum
Article Snippet: Paragraph title: RNA isolation, Northern blot, real-time RT-PCR and RACE ... The RNA was treated with RNAase-free DNase I (Promega, Madison, WI) to remove contaminating genomic DNA.

Article Title: Transcriptome profiling of drought responsive noncoding RNAs and their target genes in rice
Article Snippet: Paragraph title: Quantitative RT-PCR validation of transcript abundance ... Two hundred ng of total RNA was treated with RNAase-free DNase I (Promega), and transcribed into cDNA using gene specific RT primers and a thermostable reverse transcriptase (Invitrogen).

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA. .. Expression analysis was performed by quantitative RT–PCR in an iCycler iQ System (Bio-Rad, Hercules, CA, USA) using iQ SYBR-Green Supermix (Bio-Rad) and gene-specific primers ( ).

Expressing:

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: Paragraph title: Gene expression analysis ... Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Transcriptome profiling of drought responsive noncoding RNAs and their target genes in rice
Article Snippet: For detecting and quantifying mature miRNAs, stem-loop reverse transcription and RT-PCR of miRNAs was performed as described in Varkonyi-Gasic et al. and Chen et al. [ , ]. .. Two hundred ng of total RNA was treated with RNAase-free DNase I (Promega), and transcribed into cDNA using gene specific RT primers and a thermostable reverse transcriptase (Invitrogen).

Article Title: Comparison of inhibition of N2 fixation and ureide accumulation under water deficit in four common bean genotypes of contrasting drought tolerance
Article Snippet: .. Prior to reverse transcription (RT)–PCR, total RNA from control and treated tissues was incubated with RNAase-free DNAase I (Promega, Madison WI, USA) at 37 °C for 30 min to eliminate any traces of genomic DNA. .. Lack of amplification of 18S rRNA was used to check the successful removal of DNA.

Software:

Article Title: Transcriptome profiling of drought responsive noncoding RNAs and their target genes in rice
Article Snippet: Thermocycling and fluorescence detection were performed uisng a Strategene Mx300p real-time PCR machine and Mx3000p software version 2.02 (Stratagene). .. Two hundred ng of total RNA was treated with RNAase-free DNase I (Promega), and transcribed into cDNA using gene specific RT primers and a thermostable reverse transcriptase (Invitrogen).

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    Promega rq1 rnaase free dnase i
    Rq1 Rnaase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rq1 rnaase free dnase i/product/Promega
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rq1 rnaase free dnase i - by Bioz Stars, 2020-04
    87/100 stars
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    91
    Promega rnaase free dnase i
    Rnaase Free Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaase free dnase i/product/Promega
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rnaase free dnase i - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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