Journal: iScience
Article Title: MALAT1 regulates human macrophage metabolism by interacting with HADHB
doi: 10.1016/j.isci.2026.115107
Figure Lengend Snippet: MALAT1 binds to HADHB (A) Schematic representation of the RNA immunoprecipitation (RIP) of LPS-stimulated THP-1-derived macrophages differentiated with PMA for 24 h. (B) Western blot of RIP samples using an anti -HADHB antibody, with IgG as a negative control. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (C) Top ten candidate non-coding RNAs binding to HADHB, ranked by peak score from RIP-seq analysis. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days). (D) Fold enrichment of MALAT1 in RIP samples. The experiment was repeated; n = 3 independent experiments (independent cell cultures on different days); data are presented as mean ± SD and analyzed with the two-tailed unpaired Student’s t test.
Article Snippet: Magna RIP RNA-Binding Protein Immunoprecipitation Kit , Merck , Cat# 17-700.
Techniques: RNA Immunoprecipitation, Derivative Assay, Western Blot, Negative Control, Binding Assay, Two Tailed Test