rna extraction  (Qiagen)


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    Structured Review

    Qiagen rna extraction
    Rna Extraction, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 10306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna extraction/product/Qiagen
    Average 99 stars, based on 10306 article reviews
    Price from $9.99 to $1999.99
    rna extraction - by Bioz Stars, 2020-03
    99/100 stars

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    RNA Extraction:

    Article Title: Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
    Article Snippet: .. HCV RNA was extracted from 140 μl of plasma/serum of patient samples by manual RNA extraction, using the Qiagen Total RNA extraction kit (Qiagen, Hilden, Germany), as specified by the manufacturer. ..

    Amplification:

    Article Title: Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
    Article Snippet: Paragraph title: Amplification of HCV RNA from patient samples ... HCV RNA was extracted from 140 μl of plasma/serum of patient samples by manual RNA extraction, using the Qiagen Total RNA extraction kit (Qiagen, Hilden, Germany), as specified by the manufacturer.

    Purification:

    Article Title: Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
    Article Snippet: HCV RNA was extracted from 140 μl of plasma/serum of patient samples by manual RNA extraction, using the Qiagen Total RNA extraction kit (Qiagen, Hilden, Germany), as specified by the manufacturer. .. Each target region was amplified from 5 to 10 μl of the purified RNA solution by an RT-PCR for each target region using Transcriptor One Step RT-PCR kit (Roche Applied Science).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
    Article Snippet: HCV RNA was extracted from 140 μl of plasma/serum of patient samples by manual RNA extraction, using the Qiagen Total RNA extraction kit (Qiagen, Hilden, Germany), as specified by the manufacturer. .. Amplifications of NS3-, NS5A-, and NS5B-coding regions were performed using a RT-PCR, followed by an internal PCR with specific primers covering the three regions of interest (NS3, NS5A and NS5B) (Additional files : Figure S1, Additional file : Figure S2 and Additional file : Figure S3, and primer sequence and location are given in Additional file : Table S4, Additional file : Table S5 and Additional file : Table S6).

    Sequencing:

    Article Title: Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
    Article Snippet: HCV RNA was extracted from 140 μl of plasma/serum of patient samples by manual RNA extraction, using the Qiagen Total RNA extraction kit (Qiagen, Hilden, Germany), as specified by the manufacturer. .. Amplifications of NS3-, NS5A-, and NS5B-coding regions were performed using a RT-PCR, followed by an internal PCR with specific primers covering the three regions of interest (NS3, NS5A and NS5B) (Additional files : Figure S1, Additional file : Figure S2 and Additional file : Figure S3, and primer sequence and location are given in Additional file : Table S4, Additional file : Table S5 and Additional file : Table S6).

    other:

    Article Title: Comparison of enteroendocrine cells and pancreatic β-cells using gene expression profiling and insulin gene methylation
    Article Snippet: After total RNA extraction, the RNA was treated with DNase and subjected to a second RNA purification using the Qiagen kit (Qiagen GmbH).

    Article Title: Microbial Sensing by Intestinal Myeloid Cells Controls Carcinogenesis and Epithelial Differentiation
    Article Snippet: RNA Extraction and real-time qPCR Tissue samples were stabilized with RNAlater (QIAGEN), and RNA was extracted using the RNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions.

    Article Title: In vivo genome-wide binding interactions of mouse and human constitutive androstane receptors reveal novel gene targets
    Article Snippet: RNA extraction and quantitative real-time PCR analysis Mouse liver total RNA was extracted from WT, CAR -/-, YFP-hCAR, YFP-mCAR or YFP-empty AV infected CAR -/- mouse livers using TRIzol (Qiagen, Germantown, MD, USA), following the manufacturer's protocol.

    Article Title: An Exploratory Search for Potential Molecular Targets Responsive to the Probiotic Lactobacillus salivarius PS2 in Women With Mastitis: Gene Expression Profiling vs. Interindividual Variability
    Article Snippet: RNA extraction and purity Total RNA was isolated from all samples using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Madrid, Spain) following the manufacturer recommendations.

    Article Title: A Bordetella pertussis MgtC homolog plays a role in the intracellular survival
    Article Snippet: RNA extraction and quantitative RT-PCR (RT-qPCR) Total RNA was prepared from bacteria obtained at exponential growth phase with the RNeasy Mini Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA, USA), including a treatment with DNase I (Promega, Madison, WI).

    Article Title: Exposure to a firefighting overhaul environment without respiratory protection increases immune dysregulation and lung disease risk
    Article Snippet: RNA extraction and fragment analysis Mouse lungs were harvested 2 hrs after the OH group was removed from the overhaul environment and immediately placed in Qiagen RNAlater (Valencia, CA).

    Article Title: Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis
    Article Snippet: RNA extraction, RNase R treatment and PCR assays Total RNA was isolated from tissues and cell lines with RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions.

    Article Title: The polyphenol quercetin induces cell death in leukemia by targeting epigenetic regulators of pro-apoptotic genes
    Article Snippet: RNA extraction and real-time PCR The cells treated with Qu and the samples from the xenotransplants were collected, snap frozen, and stored at − 80 °C in RNAlater® (Qiagen, Valencia, CA, USA).

    Article Title: Characterization of the XTH Gene Family: New Insight to the Roles in Soybean Flooding Tolerance
    Article Snippet: Approximately 100 mg tissue samples were used for RNA extraction using a RNeasy Plant mini kit (Cat# 74904, Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol.

    Article Title: Nexilin/NEXN controls actin polymerization in smooth muscle and is regulated by myocardin family coactivators and YAP
    Article Snippet: RNA extraction and RT-qPCR After treatment, HBSMCs and HCASMCs were washed twice with ice-cold PBS and lysed in Qiazol (Qiagen, Cat. 79306).

    Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
    Article Snippet: RNA extraction and processing RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina).

    Article Title: Regional heterogeneity in response of airway epithelial cells to cigarette smoke
    Article Snippet: RNA extraction and real-time quantitative polymerase chain reaction (qPCR) Total RNA was extracted using miRNeasy mini kits (Qiagen, Hilden, Germany).

    Article Title: Deletion of myeloid IRS2 enhances adipose tissue sympathetic nerve function and limits obesity
    Article Snippet: CD11B positive fraction was eluted according to the manufacturer's instructions and then processed for RNA extraction using the RNeasy mini kit (Qiagen) .

    Polymerase Chain Reaction:

    Article Title: Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
    Article Snippet: HCV RNA was extracted from 140 μl of plasma/serum of patient samples by manual RNA extraction, using the Qiagen Total RNA extraction kit (Qiagen, Hilden, Germany), as specified by the manufacturer. .. Amplifications of NS3-, NS5A-, and NS5B-coding regions were performed using a RT-PCR, followed by an internal PCR with specific primers covering the three regions of interest (NS3, NS5A and NS5B) (Additional files : Figure S1, Additional file : Figure S2 and Additional file : Figure S3, and primer sequence and location are given in Additional file : Table S4, Additional file : Table S5 and Additional file : Table S6).

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