rna extraction kit  (Qiagen)


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    Structured Review

    Qiagen rna extraction kit
    PpL induces increases in Bax but not in Bcl-2 or Fas mRNA. Daudi cells were treated with PpL (100 μg/ml), OVA (100 μg/ml) or PBS. <t>RNA</t> was extracted and cDNA was obtained by <t>RT-PCR</t> and analyzed by semi-quantitative PCR for Bax, Fas, Bcl-2 and actin. Image of a representative result is shown. The OD of the bands of Bax, Bcl-2 and Fas cDNA was quantified and normalized to actin. The relative levels are shown. Data are presented as the mean ± SEM of four independent experiments, (*** p
    Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 938 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna extraction kit/product/Qiagen
    Average 99 stars, based on 938 article reviews
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    rna extraction kit - by Bioz Stars, 2020-04
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    Images

    1) Product Images from "A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells"

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0162456

    PpL induces increases in Bax but not in Bcl-2 or Fas mRNA. Daudi cells were treated with PpL (100 μg/ml), OVA (100 μg/ml) or PBS. RNA was extracted and cDNA was obtained by RT-PCR and analyzed by semi-quantitative PCR for Bax, Fas, Bcl-2 and actin. Image of a representative result is shown. The OD of the bands of Bax, Bcl-2 and Fas cDNA was quantified and normalized to actin. The relative levels are shown. Data are presented as the mean ± SEM of four independent experiments, (*** p
    Figure Legend Snippet: PpL induces increases in Bax but not in Bcl-2 or Fas mRNA. Daudi cells were treated with PpL (100 μg/ml), OVA (100 μg/ml) or PBS. RNA was extracted and cDNA was obtained by RT-PCR and analyzed by semi-quantitative PCR for Bax, Fas, Bcl-2 and actin. Image of a representative result is shown. The OD of the bands of Bax, Bcl-2 and Fas cDNA was quantified and normalized to actin. The relative levels are shown. Data are presented as the mean ± SEM of four independent experiments, (*** p

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Related Articles

    Clone Assay:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. The reverse transcription and PCR analyses were carried out using Cloned AMV Reverse Transcriptase (Invitrogen; Carlsbad, CA, USA) according to the manufacture´s protocols.

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen). .. The cDNA was subsequently used as a PCR template and cloned, as described above, using the primers KWMHC2_F (5′ CCA ATG GTA CYG AGC GGG TGA 3′) and KWMHC2_R (5′ ACG GCG CAA ACC AGC CTG T 3′), which are located within exon 2 and exon 3, respectively.

    Amplification:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. The generated cDNA were further amplified by PCR under optimized conditions using the primers pairs listed below with Recombinant Taq DNA Polymerase (Invitrogen).

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA). .. The extracted RNA was subjected to amplification of complete F and HN genes spanning the genomic region from 4498 to 6330 nucleotide through reverse transcription polymerase chain reaction (RT-PCR) using the primers and protocols as described previously (Munir et al. ).

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: Last, to confirm that both loci are transcribed in blood cells, we amplified MHC alleles from the cDNA of two individuals. .. The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen).

    Synthesized:

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. The cDNA was synthesized using Superscript II and random primers (Invitrogen) according to the manufacturer's protocol.

    Article Title: Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity
    Article Snippet: Quantitative analysis of RNA synthesis in virus-infected cells Vero cells were seeded in 6-well plates, infected with rTHOV viruses at high MOI and subjected to metabolic labelling of newly synthesized RNA at different time points after infection. .. For labelling, the cells were incubated in [3 H]-5-Uridine-containing medium (20 μCi/ml und 1 ml/well) for 1 h and lysed in the lysis buffer from RNA extraction kit with subsequent total RNA extraction (RNeasy, Qiagen).

    Quantitative RT-PCR:

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: .. 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. The cDNA was synthesized using Superscript II and random primers (Invitrogen) according to the manufacturer's protocol.

    Adsorption:

    Article Title: Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.
    Article Snippet: The cells were incubated for 1 h at 37°C to allow virus adsorption, with rocking every 10 min for uniform virus distribution. .. Viral RNA was extracted using Tripure reagent and RNA extraction kit (Qiagen, Valencia, CA, USA) as per the manufacturer's instruction.

    SYBR Green Assay:

    Article Title: Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats
    Article Snippet: .. RNA extraction kit and QuantiFast SYBR Green PCR kit were obtained from Qiagen (Hilden, Germany). .. Reverse transcription system was obtained from Thermo Scientific (Meridian Rd, Rockford, IL, USA).

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. Quantitative real-time PCR was performed on a CFX-384 Bio-Rad RT-PCR detection system using SYBR® Green Gene Expression Assays and pairs of primers designed by Sigma, as described in .

    Article Title: Hepatocyte Growth Factor Mediates the Antifibrogenic Action of Ocimum bacilicum Essential Oil against CCl4-Induced Liver Fibrosis in Rats
    Article Snippet: .. RNA extraction kit and QuantiFast SYBR Green PCR Kit were obtained from Qiagen (Hilden, Germany). .. Reverse transcription system was obtained from Thermo Scientific (Meridian Rd, Rockford, IL, USA).

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. The cDNA was used on the real-time RT² Profiler PCR Array (QIAGEN, Cat. no. PAMM-405Z) in combination with RT² SYBR® Green qPCR Mastermix (Cat. no. 330529).

    Incubation:

    Article Title: Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.
    Article Snippet: The cultures were incubated further in 5% CO 2 incubator at 37°C and observed daily for cytopathic effects (CPE) under an inverted microscope. .. Viral RNA was extracted using Tripure reagent and RNA extraction kit (Qiagen, Valencia, CA, USA) as per the manufacturer's instruction.

    Article Title: Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity
    Article Snippet: .. For labelling, the cells were incubated in [3 H]-5-Uridine-containing medium (20 μCi/ml und 1 ml/well) for 1 h and lysed in the lysis buffer from RNA extraction kit with subsequent total RNA extraction (RNeasy, Qiagen). ..

    Luciferase:

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit). .. To assay luciferase reporter activity, cells were lysed in passive lysis buffer (Promega), and luciferase activity was measured on a MicroLumat plus LB 96V luminometer from Berthold Technologies.

    Activity Assay:

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit). .. To assay luciferase reporter activity, cells were lysed in passive lysis buffer (Promega), and luciferase activity was measured on a MicroLumat plus LB 96V luminometer from Berthold Technologies.

    Cell Culture:

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: Paragraph title: Cell culture ... For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: Paragraph title: Cell culture, stimulation and lysis ... For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Expressing:

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: .. 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. The cDNA was synthesized using Superscript II and random primers (Invitrogen) according to the manufacturer's protocol.

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: .. Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. RNA quality was determined using a spectrophotometer and was reverse transcribed using a cDNA conversion kit (RT² First Strand Kit, QIAGEN, Cat. no. 330401).

    Modification:

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: We used the Z-test for selection implementing the modified Nei-Gojobori method with Jukes-Cantor correction and calculated standard errors with 1000 bootstraps. .. The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen).

    Agglutination:

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Presence of agglutinating virus was confirmed in harvested allantoic fluid by spot agglutination assay using 10 % chicken red blood cells (RBCs). .. Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA).

    HAI Assay:

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Later, identity of the isolate was confirmed as NDV through standard haemagglutination inhibition assay using specific antisera. .. Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA).

    Infection:

    Article Title: Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity
    Article Snippet: Quantitative analysis of RNA synthesis in virus-infected cells Vero cells were seeded in 6-well plates, infected with rTHOV viruses at high MOI and subjected to metabolic labelling of newly synthesized RNA at different time points after infection. .. For labelling, the cells were incubated in [3 H]-5-Uridine-containing medium (20 μCi/ml und 1 ml/well) for 1 h and lysed in the lysis buffer from RNA extraction kit with subsequent total RNA extraction (RNeasy, Qiagen).

    Generated:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. The generated cDNA were further amplified by PCR under optimized conditions using the primers pairs listed below with Recombinant Taq DNA Polymerase (Invitrogen).

    Inverted Microscopy:

    Article Title: Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.
    Article Snippet: The cultures were incubated further in 5% CO 2 incubator at 37°C and observed daily for cytopathic effects (CPE) under an inverted microscope. .. Viral RNA was extracted using Tripure reagent and RNA extraction kit (Qiagen, Valencia, CA, USA) as per the manufacturer's instruction.

    Protein Concentration:

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: The protein concentration was determined with a photo spectrometer using Bradford protein assay reagent (Pierce). .. For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: The protein concentration was determined using the Bradford method . siRNA SMARTpool On-Target plus mix (Dharmacon) of 4 siRNA oligos were used for knockdown experiments: non-targeting pool siNT (D-001810-10), TGFBR2 (LU-003930), SMAD2 (LU-003561) and SMAD4 (LU-003902). siRNA transfections were performed using Lipofectamine RNAiMax according to the manufacturer's instructions. .. For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Polymerase Chain Reaction:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. The reverse transcription and PCR analyses were carried out using Cloned AMV Reverse Transcriptase (Invitrogen; Carlsbad, CA, USA) according to the manufacture´s protocols.

    Article Title: Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats
    Article Snippet: .. RNA extraction kit and QuantiFast SYBR Green PCR kit were obtained from Qiagen (Hilden, Germany). .. Reverse transcription system was obtained from Thermo Scientific (Meridian Rd, Rockford, IL, USA).

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA). .. The amplified products were purified by 1.0 % gel electrophoresis, using the Wizard® SV Gel and PCR Clean-Up System (Promega, Co., Madison, WI, USA) as per manufacturer’s instructions.

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen). .. The cDNA was subsequently used as a PCR template and cloned, as described above, using the primers KWMHC2_F (5′ CCA ATG GTA CYG AGC GGG TGA 3′) and KWMHC2_R (5′ ACG GCG CAA ACC AGC CTG T 3′), which are located within exon 2 and exon 3, respectively.

    Article Title: Hepatocyte Growth Factor Mediates the Antifibrogenic Action of Ocimum bacilicum Essential Oil against CCl4-Induced Liver Fibrosis in Rats
    Article Snippet: .. RNA extraction kit and QuantiFast SYBR Green PCR Kit were obtained from Qiagen (Hilden, Germany). .. Reverse transcription system was obtained from Thermo Scientific (Meridian Rd, Rockford, IL, USA).

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: .. Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. RNA quality was determined using a spectrophotometer and was reverse transcribed using a cDNA conversion kit (RT² First Strand Kit, QIAGEN, Cat. no. 330401).

    Binding Assay:

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: We tested for selection by comparing the ratio of non-synonymous (dN ) over synonymous (dS ) substitutions using MEGA 5 both within and outside of the peptide binding regions. .. The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen).

    Cellular Antioxidant Activity Assay:

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen). .. The cDNA was subsequently used as a PCR template and cloned, as described above, using the primers KWMHC2_F (5′ CCA ATG GTA CYG AGC GGG TGA 3′) and KWMHC2_R (5′ ACG GCG CAA ACC AGC CTG T 3′), which are located within exon 2 and exon 3, respectively.

    Nucleic Acid Electrophoresis:

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA). .. The amplified products were purified by 1.0 % gel electrophoresis, using the Wizard® SV Gel and PCR Clean-Up System (Promega, Co., Madison, WI, USA) as per manufacturer’s instructions.

    Aqueous Normal-phase Chromatography:

    Article Title: Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brain
    Article Snippet: From non-infarcted myocardium α-MHC, β-MHC, ANP-1, SERT, 5HT2A, and 5HT2B mRNA was quantified by real-time PCR. .. RNA isolation and real-time polymerase chain reaction procedures were performed with commercially available RNA extraction kit and cDNA transcription kit (Quiagen N.V.) and TaqMan probes (Applied biosystems, Foster City) as reported previously (Hofmann et al., ).

    Transfection:

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: The protein concentration was determined using the Bradford method . siRNA SMARTpool On-Target plus mix (Dharmacon) of 4 siRNA oligos were used for knockdown experiments: non-targeting pool siNT (D-001810-10), TGFBR2 (LU-003930), SMAD2 (LU-003561) and SMAD4 (LU-003902). siRNA transfections were performed using Lipofectamine RNAiMax according to the manufacturer's instructions. .. For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Purification:

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA). .. The amplified products were purified by 1.0 % gel electrophoresis, using the Wizard® SV Gel and PCR Clean-Up System (Promega, Co., Madison, WI, USA) as per manufacturer’s instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: .. Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. RNA quantification was assessed with Gene Quant Pro (Amersham Biosciences; GE Healthcare, Buckinghamshire, UK).

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. Quantitative real-time PCR was performed on a CFX-384 Bio-Rad RT-PCR detection system using SYBR® Green Gene Expression Assays and pairs of primers designed by Sigma, as described in .

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA). .. The extracted RNA was subjected to amplification of complete F and HN genes spanning the genomic region from 4498 to 6330 nucleotide through reverse transcription polymerase chain reaction (RT-PCR) using the primers and protocols as described previously (Munir et al. ).

    Metabolic Labelling:

    Article Title: Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity
    Article Snippet: Quantitative analysis of RNA synthesis in virus-infected cells Vero cells were seeded in 6-well plates, infected with rTHOV viruses at high MOI and subjected to metabolic labelling of newly synthesized RNA at different time points after infection. .. For labelling, the cells were incubated in [3 H]-5-Uridine-containing medium (20 μCi/ml und 1 ml/well) for 1 h and lysed in the lysis buffer from RNA extraction kit with subsequent total RNA extraction (RNeasy, Qiagen).

    Bradford Protein Assay:

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: The protein concentration was determined with a photo spectrometer using Bradford protein assay reagent (Pierce). .. For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Mouse Assay:

    Article Title: Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brain
    Article Snippet: Sham operated mice had no signs of myocardial infarction. .. RNA isolation and real-time polymerase chain reaction procedures were performed with commercially available RNA extraction kit and cDNA transcription kit (Quiagen N.V.) and TaqMan probes (Applied biosystems, Foster City) as reported previously (Hofmann et al., ).

    RNA Extraction:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: .. Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. RNA quantification was assessed with Gene Quant Pro (Amersham Biosciences; GE Healthcare, Buckinghamshire, UK).

    Article Title: Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats
    Article Snippet: .. RNA extraction kit and QuantiFast SYBR Green PCR kit were obtained from Qiagen (Hilden, Germany). .. Reverse transcription system was obtained from Thermo Scientific (Meridian Rd, Rockford, IL, USA).

    Article Title: Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.
    Article Snippet: .. Viral RNA was extracted using Tripure reagent and RNA extraction kit (Qiagen, Valencia, CA, USA) as per the manufacturer's instruction. ..

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: .. 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. The cDNA was synthesized using Superscript II and random primers (Invitrogen) according to the manufacturer's protocol.

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: .. Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA). .. Quantity (NanoDrop, USA) and quality (QubitFlourometer, USA) of extracted RNA was measured.

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: .. The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen). .. The cDNA was subsequently used as a PCR template and cloned, as described above, using the primers KWMHC2_F (5′ CCA ATG GTA CYG AGC GGG TGA 3′) and KWMHC2_R (5′ ACG GCG CAA ACC AGC CTG T 3′), which are located within exon 2 and exon 3, respectively.

    Article Title: Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity
    Article Snippet: .. For labelling, the cells were incubated in [3 H]-5-Uridine-containing medium (20 μCi/ml und 1 ml/well) for 1 h and lysed in the lysis buffer from RNA extraction kit with subsequent total RNA extraction (RNeasy, Qiagen). ..

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: .. For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit). .. To assay luciferase reporter activity, cells were lysed in passive lysis buffer (Promega), and luciferase activity was measured on a MicroLumat plus LB 96V luminometer from Berthold Technologies.

    Article Title: Hepatocyte Growth Factor Mediates the Antifibrogenic Action of Ocimum bacilicum Essential Oil against CCl4-Induced Liver Fibrosis in Rats
    Article Snippet: .. RNA extraction kit and QuantiFast SYBR Green PCR Kit were obtained from Qiagen (Hilden, Germany). .. Reverse transcription system was obtained from Thermo Scientific (Meridian Rd, Rockford, IL, USA).

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: .. Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. RNA quality was determined using a spectrophotometer and was reverse transcribed using a cDNA conversion kit (RT² First Strand Kit, QIAGEN, Cat. no. 330401).

    Article Title: Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brain
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction procedures were performed with commercially available RNA extraction kit and cDNA transcription kit (Quiagen N.V.) and TaqMan probes (Applied biosystems, Foster City) as reported previously (Hofmann et al., ). ..

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: .. For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit). .. Luciferase assay For luciferase assays, cells were lysed, after washing twice with 1xPBS, by adding passive lysis buffer (Promega) directly onto the plates following the manufacturer’s protocol.

    Isolation:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: .. Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. RNA quantification was assessed with Gene Quant Pro (Amersham Biosciences; GE Healthcare, Buckinghamshire, UK).

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: .. 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. The cDNA was synthesized using Superscript II and random primers (Invitrogen) according to the manufacturer's protocol.

    Article Title: Genetic characterization and phylogeny of pigeon paramyxovirus isolate (PPMV-1) from Pakistan
    Article Snippet: Necropsy was performed and samples (trachea, lungs, and brain) were processed for isolation of NDV in 9 day old embryonated chicken eggs (ECE). .. Total RNA was extracted from allantoic fluid using commercially available RNA extraction kit as per manufacturer’s instructions (QIAamp Viral RNA Mini Kit, Qiagen, USA).

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: .. For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit). .. To assay luciferase reporter activity, cells were lysed in passive lysis buffer (Promega), and luciferase activity was measured on a MicroLumat plus LB 96V luminometer from Berthold Technologies.

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: .. Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. RNA quality was determined using a spectrophotometer and was reverse transcribed using a cDNA conversion kit (RT² First Strand Kit, QIAGEN, Cat. no. 330401).

    Article Title: Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brain
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction procedures were performed with commercially available RNA extraction kit and cDNA transcription kit (Quiagen N.V.) and TaqMan probes (Applied biosystems, Foster City) as reported previously (Hofmann et al., ). ..

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: .. For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit). .. Luciferase assay For luciferase assays, cells were lysed, after washing twice with 1xPBS, by adding passive lysis buffer (Promega) directly onto the plates following the manufacturer’s protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. Quantitative real-time PCR was performed on a CFX-384 Bio-Rad RT-PCR detection system using SYBR® Green Gene Expression Assays and pairs of primers designed by Sigma, as described in .

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. The cDNA was used on the real-time RT² Profiler PCR Array (QIAGEN, Cat. no. PAMM-405Z) in combination with RT² SYBR® Green qPCR Mastermix (Cat. no. 330529).

    Article Title: Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brain
    Article Snippet: .. RNA isolation and real-time polymerase chain reaction procedures were performed with commercially available RNA extraction kit and cDNA transcription kit (Quiagen N.V.) and TaqMan probes (Applied biosystems, Foster City) as reported previously (Hofmann et al., ). ..

    Negative Control:

    Article Title: PI3K p110β subunit in leptin receptor expressing cells is required for the acute hypophagia induced by endotoxemia
    Article Snippet: 2.5.6 Gene expression by real time RT-PCR Total RNA was isolated from MBH or ARC punches using RNA extraction kit, Qiazol Reagent (miRNeasy, Qiagen) and DNase treatment (RNase-Free; Qiagen). .. Water instead of cDNA was used as a negative control.

    Recombinant:

    Article Title: A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells
    Article Snippet: Total RNA isolation and RT-PCR Total RNA was isolated using the RNA extraction Kit following the manufacturer´s instructions (Qiagen; Limburgo ). .. The generated cDNA were further amplified by PCR under optimized conditions using the primers pairs listed below with Recombinant Taq DNA Polymerase (Invitrogen).

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: Human recombinant TGFβ1 (R & D Systems) was diluted in a solution containing 4 mM HCl and 0.1% BSA. .. For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Selection:

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: We used the Z-test for selection implementing the modified Nei-Gojobori method with Jukes-Cantor correction and calculated standard errors with 1000 bootstraps. .. The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen).

    Functional Assay:

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: First, we searched for 1) the presence of frame shift mutations or stop codons in the translated alleles and 2) characteristic features of functional Class II molecules, such as conserved residues and putative peptide binding regions (inferred from ). .. The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen).

    Spectrophotometry:

    Article Title: Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
    Article Snippet: Gene expression analysis – PCR array Total RNA was isolated from all different cell populations using an RNA extraction kit according to the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN, Cat. no. 74104). .. RNA quality was determined using a spectrophotometer and was reverse transcribed using a cDNA conversion kit (RT² First Strand Kit, QIAGEN, Cat. no. 330401).

    Virus Isolation Assay:

    Article Title: Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.
    Article Snippet: Paragraph title: Virus isolation from bat tissue specimens ... Viral RNA was extracted using Tripure reagent and RNA extraction kit (Qiagen, Valencia, CA, USA) as per the manufacturer's instruction.

    CTG Assay:

    Article Title: Preen secretions encode information on MHC similarity in certain sex-dyads in a monogamous seabird
    Article Snippet: The RNA was subsequently treated with DNase that was provided with the RNA extraction kit and reverse transcribed using a QuantiTect Reverse Transcription Kit (Qiagen). .. The cDNA was subsequently used as a PCR template and cloned, as described above, using the primers KWMHC2_F (5′ CCA ATG GTA CYG AGC GGG TGA 3′) and KWMHC2_R (5′ ACG GCG CAA ACC AGC CTG T 3′), which are located within exon 2 and exon 3, respectively.

    Lysis:

    Article Title: Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity
    Article Snippet: .. For labelling, the cells were incubated in [3 H]-5-Uridine-containing medium (20 μCi/ml und 1 ml/well) for 1 h and lysed in the lysis buffer from RNA extraction kit with subsequent total RNA extraction (RNeasy, Qiagen). ..

    Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
    Article Snippet: For lysis, cells were scraped on ice in lysis buffer (50 mM Tris–HCl pH 7.5, 0.27 M sucrose, 150 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1 mM sodium orthovanadate, 1 mM sodium β-glycerophosphate, 50 mM sodium fluoride, 5 mM sodium pyrophosphate, 1% Triton X-100 and 0.5% Nonidet P-40) supplemented with complete protease inhibitors (one tablet per 25 ml; Roche) and 0.1% β-mercaptoethanol (Sigma) or 50 mM iodoacetamide (Sigma) or 2.5 mg ml−1 dithiobis[succinimidyl propionate] (DSP) (Thermo Scientific). .. For RNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

    Article Title: Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
    Article Snippet: Paragraph title: Cell culture, stimulation and lysis ... For mRNA isolation, cells were processed using an RNA extraction kit according to the manufacturer’s instructions (Qiagen RNeasy kit).

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    Qiagen magattract virus mini m48 kits
    Magattract Virus Mini M48 Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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