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R&D Systems rmil 4
HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and <t>rmIL-4</t> or with CD154 and rmIL-4
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Images

1) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

2) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

3) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

4) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

5) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

6) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

7) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

8) Product Images from "Epithelial Cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is Crucial for Murine Asthma"

Article Title: Epithelial Cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is Crucial for Murine Asthma

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi: 10.4049/jimmunol.1200461

IL-25 can induce chemokine production by airway epithelial cells. The levels of CCL5 and CXCL1 in the culture supernatants and cell lysates of airway epithelial cells after stimulation with the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or
Figure Legend Snippet: IL-25 can induce chemokine production by airway epithelial cells. The levels of CCL5 and CXCL1 in the culture supernatants and cell lysates of airway epithelial cells after stimulation with the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or

Techniques Used:

IL-25 can potently induce airway epithelial cell and eosinophil activation. A , B , Chemokine expression/production by MLE-12 cultured in the presence and absence of the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or 100-ng/ml rmIL-13 for 4 hours
Figure Legend Snippet: IL-25 can potently induce airway epithelial cell and eosinophil activation. A , B , Chemokine expression/production by MLE-12 cultured in the presence and absence of the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or 100-ng/ml rmIL-13 for 4 hours

Techniques Used: Activation Assay, Expressing, Cell Culture

9) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

10) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

11) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

12) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

13) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

14) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

15) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

16) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

17) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

18) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

19) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

20) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

21) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

22) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

23) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M110.169086

HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

Techniques Used: Expressing

24) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

Journal: Molecular immunology

doi: 10.1016/j.molimm.2010.10.023

Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

Techniques Used: Amplification, Clone Assay

DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

Techniques Used: Labeling, In Situ

Related Articles

Flow Cytometry:

Article Title: Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching
Article Snippet: .. Positively transduced cells were then selected with 0.6 μg/mL puromycin (Wisent Bio Products) and stimulated with 25 ng/mL recombinant mouse IL-4 (R & D Systems) for 4 days, and then analyzed by flow cytometry. .. Experimental protocols with mice were carried out in accordance with the University of Toronto Division of Comparative Medicine guidelines and regulations and were approved using protocol number 20011472.

In Vitro:

Article Title: Effect of fermented soybean products intake on the overall immune safety and function in mice
Article Snippet: .. Splenic B cells (106 cells) were stimulated in vitro with a mixture of lipopolysaccharide (1 µg; Sigma), recombinant mouse IL-4 (50 ng; R & D Systems, USA), and recombinant human APRIL (10 ng; R & D Systems) for 96 h at 37℃ in a 5% CO2 incubator [ ]. .. The culture medium was BioWhittaker RPMI1640 (Lonza Cologne, USA) supplemented with nonessential amino acid (1 mM), sodium pyruvate (1 mM), sodium bicarbonate (1%), glutamine (2 mM), 2-mercaptoethanol (50 µM), and 10% heat-inactivated fetal bovine serum (HyClone, USA).

Cytometry:

Article Title: Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching
Article Snippet: .. Positively transduced cells were then selected with 0.6 μg/mL puromycin (Wisent Bio Products) and stimulated with 25 ng/mL recombinant mouse IL-4 (R & D Systems) for 4 days, and then analyzed by flow cytometry. .. Experimental protocols with mice were carried out in accordance with the University of Toronto Division of Comparative Medicine guidelines and regulations and were approved using protocol number 20011472.

Cell Culture:

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *
Article Snippet: .. In addition, consistent with our finding that estrogen alone did not significantly induce HoxC4 expression but powerfully potentiated CD154- or LPS-induced HoxC4 expression and HoxC4-mediated AID induction, an Ab to HoxC4 precipitated a significantly greater amount of Aicda promoter DNA in primary mouse B cells induced by LPS and rmIL-4 in the presence of E2 as compared with B cells cultured under similar conditions but in the absence of E2 or B cells cultured with E2 alone or nil. .. To further demonstrate that HoxC4 binds to the conserved HOXC4/HoxC4 promoter EREs, we performed EMSAs, using oligonucleotides encompassing the three individual EREs of the human HOXC4 promoter sequence or oligonucleotides with disrupted ERE sequences as probes ( C ).

Expressing:

Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *
Article Snippet: .. In addition, consistent with our finding that estrogen alone did not significantly induce HoxC4 expression but powerfully potentiated CD154- or LPS-induced HoxC4 expression and HoxC4-mediated AID induction, an Ab to HoxC4 precipitated a significantly greater amount of Aicda promoter DNA in primary mouse B cells induced by LPS and rmIL-4 in the presence of E2 as compared with B cells cultured under similar conditions but in the absence of E2 or B cells cultured with E2 alone or nil. .. To further demonstrate that HoxC4 binds to the conserved HOXC4/HoxC4 promoter EREs, we performed EMSAs, using oligonucleotides encompassing the three individual EREs of the human HOXC4 promoter sequence or oligonucleotides with disrupted ERE sequences as probes ( C ).

Recombinant:

Article Title: Interleukin-4 Inhibits RANKL-Induced NFATc1 Expression Via STAT6: A Novel Mechanism Mediating its Blockade of Osteoclastogenesis
Article Snippet: .. Recombinant murine IL-4 was purchased from R & D Systems, Inc. (Minneapolis, MN). .. Polyclonal anti-IκBα, anti-phospho-IκBα, anti-p44/42ERK, anti-phospho-p44/42ERK, anti-JNK, anti-phospho-JNK, anti-p38, and anti-phospho-p38 were all purchased from Cell Signaling Technology, Inc. (Beverly, MA).

Article Title: Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching
Article Snippet: .. Positively transduced cells were then selected with 0.6 μg/mL puromycin (Wisent Bio Products) and stimulated with 25 ng/mL recombinant mouse IL-4 (R & D Systems) for 4 days, and then analyzed by flow cytometry. .. Experimental protocols with mice were carried out in accordance with the University of Toronto Division of Comparative Medicine guidelines and regulations and were approved using protocol number 20011472.

Article Title: Role of NADPH oxidase in interleukin-4-induced monocyte chemoattractant protein-1 expression in vascular endothelium
Article Snippet: .. Recombinant human and mouse IL-4 were purchased from R & D Systems Inc. (Minneapolis, MN) and dissolved in sterile phosphate buffered saline (PBS). ..

Article Title: Tumor growth suppressive effect of IL-4 through p21-mediated activation of STAT6 in IL-4Rα overexpressed melanoma models
Article Snippet: .. Materials Recombinant human IL-4 and mouse IL-4 were purchased from R & D system (Minneapolis , Minnesota ). .. Cell culture SK-MEL-28 human melanoma cells were obtained from the Korean Cell Line Bank (Seoul, Korea).

Article Title: Effect of fermented soybean products intake on the overall immune safety and function in mice
Article Snippet: .. Splenic B cells (106 cells) were stimulated in vitro with a mixture of lipopolysaccharide (1 µg; Sigma), recombinant mouse IL-4 (50 ng; R & D Systems, USA), and recombinant human APRIL (10 ng; R & D Systems) for 96 h at 37℃ in a 5% CO2 incubator [ ]. .. The culture medium was BioWhittaker RPMI1640 (Lonza Cologne, USA) supplemented with nonessential amino acid (1 mM), sodium pyruvate (1 mM), sodium bicarbonate (1%), glutamine (2 mM), 2-mercaptoethanol (50 µM), and 10% heat-inactivated fetal bovine serum (HyClone, USA).

Article Title: IKKβ Activity Drives Fetal Lung Macrophage Maturation Along a Non-M1/M2 Paradigm
Article Snippet: .. Recombinant mouse IL-4 and IL-13 were purchased from R & D Systems. .. RPMI 1640 media was purchased from Invitrogen, and FBS was purchased from Thermo Fisher Scientific.

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  • 93
    R&D Systems recombinant murine il 4
    Effects of <t>IL-4</t> and IL-13 on NFATc1 mRNA expression in osteoclast precursors. BMMs were treated with or without 150 ng/ml of RANKL in the presence of IL-4 or IL-13 at the indicated concentrations for 24 hr. Total RNA was then extracted and NFATc1 mRNA
    Recombinant Murine Il 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant murine il 4/product/R&D Systems
    Average 93 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    recombinant murine il 4 - by Bioz Stars, 2020-11
    93/100 stars
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    94
    R&D Systems recombinant il 4
    Immunophenotyping of monocyte and monocyte-derived cells in the absence of Human amniotic epithelial cells. (A) Immunophenotype profile of monocytes (Day 0), immature dendritic cells (day 5) and mature dendritic cells (day 7). Monocytes were isolated by magnetic activated cell sorting and were differentiated to immature dendritic cells s by the use of <t>IL-4</t> and GM-CSF for five days. Then, immature dendritic cells were stimulated by adding 100 ng/ml LPS and incubated for two more days (day 7). Immunophenotype of cells were measured using flow cytometry. Open histograms display cells stained with monoclonal antibodies and filled histograms display cells stained with isotype-matched control antibodies. experiments. (B) The figure displays the percentages of cells staining positive for each marker; * indicates statistically significant differences of cells. iDC: immature dendritic cells, mDC: mature dendritic cells.
    Recombinant Il 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant il 4/product/R&D Systems
    Average 94 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    recombinant il 4 - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

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    Effects of IL-4 and IL-13 on NFATc1 mRNA expression in osteoclast precursors. BMMs were treated with or without 150 ng/ml of RANKL in the presence of IL-4 or IL-13 at the indicated concentrations for 24 hr. Total RNA was then extracted and NFATc1 mRNA

    Journal:

    Article Title: Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts

    doi: 10.1111/j.1365-2567.2006.02538.x

    Figure Lengend Snippet: Effects of IL-4 and IL-13 on NFATc1 mRNA expression in osteoclast precursors. BMMs were treated with or without 150 ng/ml of RANKL in the presence of IL-4 or IL-13 at the indicated concentrations for 24 hr. Total RNA was then extracted and NFATc1 mRNA

    Article Snippet: Recombinant murine IL-4 and IL-13, and transforming growth factor-β (TGF-β) were purchased from R & D systems (Minneapolis, MN).

    Techniques: Expressing

    Effects of IL-4 and IL-13 on osteoclast differentiation in a coculture system. (a) BMMs were cocultured with UAMS-32 cells and various concentrations of IL-4 (○) or IL-13 (▵) in the presence of 100 n m 1α,25-(OH) 2 D 3 . The same cultures

    Journal:

    Article Title: Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts

    doi: 10.1111/j.1365-2567.2006.02538.x

    Figure Lengend Snippet: Effects of IL-4 and IL-13 on osteoclast differentiation in a coculture system. (a) BMMs were cocultured with UAMS-32 cells and various concentrations of IL-4 (○) or IL-13 (▵) in the presence of 100 n m 1α,25-(OH) 2 D 3 . The same cultures

    Article Snippet: Recombinant murine IL-4 and IL-13, and transforming growth factor-β (TGF-β) were purchased from R & D systems (Minneapolis, MN).

    Techniques:

    Phosphorylation of STAT6 by IL-4 and IL-13 in UAMS-32 cells and BMMs. UAMS-32 (a) cells and BMMs (b) were treated with 0·1, 1, 10 and 10 ng/ml of IL-4 and IL-13 for 30 min. Subsequently, phosphorylation of STAT6 protein was analysed by Western

    Journal:

    Article Title: Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts

    doi: 10.1111/j.1365-2567.2006.02538.x

    Figure Lengend Snippet: Phosphorylation of STAT6 by IL-4 and IL-13 in UAMS-32 cells and BMMs. UAMS-32 (a) cells and BMMs (b) were treated with 0·1, 1, 10 and 10 ng/ml of IL-4 and IL-13 for 30 min. Subsequently, phosphorylation of STAT6 protein was analysed by Western

    Article Snippet: Recombinant murine IL-4 and IL-13, and transforming growth factor-β (TGF-β) were purchased from R & D systems (Minneapolis, MN).

    Techniques: Western Blot

    Expression of receptor components for IL-4 and IL-13 in osteoclast precursors and osteoblastic cells. Total RNAs were extracted from BMCs, primary osteoblasts (OBs), BMMs, RAW264.7 cells, UAMS-32 cells, and ST-2 cells. Subsequently, the mRNA expressions

    Journal:

    Article Title: Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts

    doi: 10.1111/j.1365-2567.2006.02538.x

    Figure Lengend Snippet: Expression of receptor components for IL-4 and IL-13 in osteoclast precursors and osteoblastic cells. Total RNAs were extracted from BMCs, primary osteoblasts (OBs), BMMs, RAW264.7 cells, UAMS-32 cells, and ST-2 cells. Subsequently, the mRNA expressions

    Article Snippet: Recombinant murine IL-4 and IL-13, and transforming growth factor-β (TGF-β) were purchased from R & D systems (Minneapolis, MN).

    Techniques: Expressing

    Effects of IL-4 and IL-13 on osteoclast differentiation induced by RANKL in osteoclast precursor cultures. (a) BMMs were cultured with various concentrations of IL-4 with (○) or without (•) 150 ng/ml of RANKL, or IL-13 with (▵)

    Journal:

    Article Title: Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts

    doi: 10.1111/j.1365-2567.2006.02538.x

    Figure Lengend Snippet: Effects of IL-4 and IL-13 on osteoclast differentiation induced by RANKL in osteoclast precursor cultures. (a) BMMs were cultured with various concentrations of IL-4 with (○) or without (•) 150 ng/ml of RANKL, or IL-13 with (▵)

    Article Snippet: Recombinant murine IL-4 and IL-13, and transforming growth factor-β (TGF-β) were purchased from R & D systems (Minneapolis, MN).

    Techniques: Cell Culture

    Regulation of OPG and RANKL gene expression by IL-4 and IL-13 in osteoblastic cells. (a) Dose effects of IL-4 and IL-13 on the induction of OPG mRNA. UAMS-32 cells were treated with 1, 10, and 100 ng/ml of IL-4 or IL-13 for 3 hr. (b) Time course analysis

    Journal:

    Article Title: Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts

    doi: 10.1111/j.1365-2567.2006.02538.x

    Figure Lengend Snippet: Regulation of OPG and RANKL gene expression by IL-4 and IL-13 in osteoblastic cells. (a) Dose effects of IL-4 and IL-13 on the induction of OPG mRNA. UAMS-32 cells were treated with 1, 10, and 100 ng/ml of IL-4 or IL-13 for 3 hr. (b) Time course analysis

    Article Snippet: Recombinant murine IL-4 and IL-13, and transforming growth factor-β (TGF-β) were purchased from R & D systems (Minneapolis, MN).

    Techniques: Expressing

    NF-κB activation in fetal mouse lung macrophages stimulated a proinflammatory response and reduced the response to IL-4 and IL-13. IKFM mice expressing a tetracyclineinducible constitutively active IKKβ mutant were given doxycycline from E14-E18. Fetal lung macrophages from E18 IKFM fetal mouse lungs and littermate controls were cultured with IL-4 or IL-13. ( A-C ) RNA was isolated and expression of proinflammatory markers (CXCL10, CCL-3, IL-1β, TNFα) and alternative activation markers (CCL-17, CD206) were measured by real time PCR (□ p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IKKβ Activity Drives Fetal Lung Macrophage Maturation Along a Non-M1/M2 Paradigm

    doi: 10.4049/jimmunol.1302516

    Figure Lengend Snippet: NF-κB activation in fetal mouse lung macrophages stimulated a proinflammatory response and reduced the response to IL-4 and IL-13. IKFM mice expressing a tetracyclineinducible constitutively active IKKβ mutant were given doxycycline from E14-E18. Fetal lung macrophages from E18 IKFM fetal mouse lungs and littermate controls were cultured with IL-4 or IL-13. ( A-C ) RNA was isolated and expression of proinflammatory markers (CXCL10, CCL-3, IL-1β, TNFα) and alternative activation markers (CCL-17, CD206) were measured by real time PCR (□ p

    Article Snippet: Recombinant mouse IL-4 and IL-13 were purchased from R & D Systems.

    Techniques: Activation Assay, Mouse Assay, Expressing, Mutagenesis, Cell Culture, Isolation, Real-time Polymerase Chain Reaction

    Transcriptional response of fetal mouse lung macrophages in response to LPS, IL-4, and IL-13. (A) Macrophages were isolated from E15 fetal C57/BL6 mouse lungs and treated with LPS (250 ng/ml), IL-4 (5 ng/ml), and IL-13 (10 ng/ml) for 4 h as indicated. Following RNA isolation, real time PCR measured expression of proinflammatory markers (CXCL10, CCL-3, IL-1β, TNFα) and alternative activation markers (CCL-17, CD206). For each marker, expression was compared to control, untreated samples (* p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IKKβ Activity Drives Fetal Lung Macrophage Maturation Along a Non-M1/M2 Paradigm

    doi: 10.4049/jimmunol.1302516

    Figure Lengend Snippet: Transcriptional response of fetal mouse lung macrophages in response to LPS, IL-4, and IL-13. (A) Macrophages were isolated from E15 fetal C57/BL6 mouse lungs and treated with LPS (250 ng/ml), IL-4 (5 ng/ml), and IL-13 (10 ng/ml) for 4 h as indicated. Following RNA isolation, real time PCR measured expression of proinflammatory markers (CXCL10, CCL-3, IL-1β, TNFα) and alternative activation markers (CCL-17, CD206). For each marker, expression was compared to control, untreated samples (* p

    Article Snippet: Recombinant mouse IL-4 and IL-13 were purchased from R & D Systems.

    Techniques: Isolation, Real-time Polymerase Chain Reaction, Expressing, Activation Assay, Marker

    Effect of a 4 week experimental diet of doenjang (DJ), cheonggukjang (CGJ), or unfermented raw material mixture (UF) on interferon-γ (IFN-γ) and interleukin (IL)-4 production from splenic T cells stimulated ex vivo compared with standard rodent chow intake (Plain). The IFN-γ IL-4 ratio was calculated by dividing the IFN-γ level with the IL-4 level and then multiplying the result by 100 for each individual culture supernatant. Values are expressed as the mean ± SEM for seven mice per group. * p

    Journal: Journal of Veterinary Science

    Article Title: Effect of fermented soybean products intake on the overall immune safety and function in mice

    doi: 10.4142/jvs.2017.18.1.25

    Figure Lengend Snippet: Effect of a 4 week experimental diet of doenjang (DJ), cheonggukjang (CGJ), or unfermented raw material mixture (UF) on interferon-γ (IFN-γ) and interleukin (IL)-4 production from splenic T cells stimulated ex vivo compared with standard rodent chow intake (Plain). The IFN-γ IL-4 ratio was calculated by dividing the IFN-γ level with the IL-4 level and then multiplying the result by 100 for each individual culture supernatant. Values are expressed as the mean ± SEM for seven mice per group. * p

    Article Snippet: Splenic B cells (106 cells) were stimulated in vitro with a mixture of lipopolysaccharide (1 µg; Sigma), recombinant mouse IL-4 (50 ng; R & D Systems, USA), and recombinant human APRIL (10 ng; R & D Systems) for 96 h at 37℃ in a 5% CO2 incubator [ ].

    Techniques: Raw Material, Ex Vivo, Mouse Assay

    Immunophenotyping of monocyte and monocyte-derived cells in the absence of Human amniotic epithelial cells. (A) Immunophenotype profile of monocytes (Day 0), immature dendritic cells (day 5) and mature dendritic cells (day 7). Monocytes were isolated by magnetic activated cell sorting and were differentiated to immature dendritic cells s by the use of IL-4 and GM-CSF for five days. Then, immature dendritic cells were stimulated by adding 100 ng/ml LPS and incubated for two more days (day 7). Immunophenotype of cells were measured using flow cytometry. Open histograms display cells stained with monoclonal antibodies and filled histograms display cells stained with isotype-matched control antibodies. experiments. (B) The figure displays the percentages of cells staining positive for each marker; * indicates statistically significant differences of cells. iDC: immature dendritic cells, mDC: mature dendritic cells.

    Journal: International Journal of Reproductive Biomedicine

    Article Title: The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study

    doi: 10.18502/ijrm.v13i6.7286

    Figure Lengend Snippet: Immunophenotyping of monocyte and monocyte-derived cells in the absence of Human amniotic epithelial cells. (A) Immunophenotype profile of monocytes (Day 0), immature dendritic cells (day 5) and mature dendritic cells (day 7). Monocytes were isolated by magnetic activated cell sorting and were differentiated to immature dendritic cells s by the use of IL-4 and GM-CSF for five days. Then, immature dendritic cells were stimulated by adding 100 ng/ml LPS and incubated for two more days (day 7). Immunophenotype of cells were measured using flow cytometry. Open histograms display cells stained with monoclonal antibodies and filled histograms display cells stained with isotype-matched control antibodies. experiments. (B) The figure displays the percentages of cells staining positive for each marker; * indicates statistically significant differences of cells. iDC: immature dendritic cells, mDC: mature dendritic cells.

    Article Snippet: Recombinant IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were obtained from R & D (USA).

    Techniques: Derivative Assay, Isolation, FACS, Incubation, Flow Cytometry, Staining, Marker

    Immunophenotyping of monocyte-derived immature dendritic cells in the presence and absence of Human amniotic epithelial cells. Monocytes cultured with GM-CSF plus IL-4 for three-five days in the presence of Human amniotic epithelial cells in Transwell. Monocyte cells were cultured at ratios 1:1, 1:2.5, and 1:10 (monocytes: hAECs). [hAECs-iDCs] were analyzed for markers shown in the figure using flow cytometry. (A) The percentages of cells staining positive for CD1a and CD14. (B) The mean of Mean Ratio Fluorescence Intensity for each marker. Data are expressed as mean ± SD of four separate experiments. [hAECs-iDCs]: monocyte derived immature dendritic cells in the presence of Human amniotic epithelial cells. MRFI: Mean Ratio Fluorescence Intensity. iDC: monocyte derived immature dendritic cells in the absence of Human amniotic epithelial cells.

    Journal: International Journal of Reproductive Biomedicine

    Article Title: The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study

    doi: 10.18502/ijrm.v13i6.7286

    Figure Lengend Snippet: Immunophenotyping of monocyte-derived immature dendritic cells in the presence and absence of Human amniotic epithelial cells. Monocytes cultured with GM-CSF plus IL-4 for three-five days in the presence of Human amniotic epithelial cells in Transwell. Monocyte cells were cultured at ratios 1:1, 1:2.5, and 1:10 (monocytes: hAECs). [hAECs-iDCs] were analyzed for markers shown in the figure using flow cytometry. (A) The percentages of cells staining positive for CD1a and CD14. (B) The mean of Mean Ratio Fluorescence Intensity for each marker. Data are expressed as mean ± SD of four separate experiments. [hAECs-iDCs]: monocyte derived immature dendritic cells in the presence of Human amniotic epithelial cells. MRFI: Mean Ratio Fluorescence Intensity. iDC: monocyte derived immature dendritic cells in the absence of Human amniotic epithelial cells.

    Article Snippet: Recombinant IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were obtained from R & D (USA).

    Techniques: Derivative Assay, Cell Culture, Flow Cytometry, Staining, Fluorescence, Marker

    Immunophenotyping of monocyte-derived mature dendritic cells in the presence and absence of Human amniotic epithelial cells. Monocytes were cultured with GM-CSF plus IL-4 for three-five days in the presence of human amniotic membrane in Transwell. Then, immature dendritic cells were stimulated by adding 100 ng/ml LPS and incubated for two more days (day 7). The cells from this co-culture were named [hAECs-iDCs] + LPS. Monocyte cells were cultured at ratios 1:1, 1:2.5, and 1:10 (monocytes: human amniotic membrane). [hAECs-iDCs] + LPS were analyzed for markers shown in the figure using flow cytometry. (A) The percentages of cells staining positive for CD1a and CD14. (B) The mean of Mean Ratio Fluorescence Intensity for each marker. Data are expressed as mean ± SD of four separate experiments. The Kruskal-Wallis test was used to analyze. MRFI: Mean Ratio Fluorescence Intensity. mDC: monocyte derived immature dendritic cells in the absence of Human amniotic epithelial cells and then maturated with LPS for two days.

    Journal: International Journal of Reproductive Biomedicine

    Article Title: The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study

    doi: 10.18502/ijrm.v13i6.7286

    Figure Lengend Snippet: Immunophenotyping of monocyte-derived mature dendritic cells in the presence and absence of Human amniotic epithelial cells. Monocytes were cultured with GM-CSF plus IL-4 for three-five days in the presence of human amniotic membrane in Transwell. Then, immature dendritic cells were stimulated by adding 100 ng/ml LPS and incubated for two more days (day 7). The cells from this co-culture were named [hAECs-iDCs] + LPS. Monocyte cells were cultured at ratios 1:1, 1:2.5, and 1:10 (monocytes: human amniotic membrane). [hAECs-iDCs] + LPS were analyzed for markers shown in the figure using flow cytometry. (A) The percentages of cells staining positive for CD1a and CD14. (B) The mean of Mean Ratio Fluorescence Intensity for each marker. Data are expressed as mean ± SD of four separate experiments. The Kruskal-Wallis test was used to analyze. MRFI: Mean Ratio Fluorescence Intensity. mDC: monocyte derived immature dendritic cells in the absence of Human amniotic epithelial cells and then maturated with LPS for two days.

    Article Snippet: Recombinant IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were obtained from R & D (USA).

    Techniques: Derivative Assay, Cell Culture, Incubation, Co-Culture Assay, Flow Cytometry, Staining, Fluorescence, Marker