rmil 4  (R&D Systems)

 
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    Name:
    Recombinant Mouse IL 4 Protein CF
    Description:
    The Recombinant Mouse IL 4 Protein from R D Systems is derived from E coli The Recombinant Mouse IL 4 Protein has been validated for the following applications Bioactivity
    Catalog Number:
    404-ML-010/CF
    Price:
    209
    Category:
    Proteins and Enzymes
    Source:
    E. coli-derived Recombinant Mouse IL-4 Protein
    Applications:
    Bioactivity
    Purity:
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
    Conjugate:
    Unconjugated
    Size:
    10 ug
    Buy from Supplier


    Structured Review

    R&D Systems rmil 4
    Recombinant Mouse IL 4 Protein CF
    The Recombinant Mouse IL 4 Protein from R D Systems is derived from E coli The Recombinant Mouse IL 4 Protein has been validated for the following applications Bioactivity
    https://www.bioz.com/result/rmil 4/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rmil 4 - by Bioz Stars, 2021-05
    94/100 stars

    Images

    1) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    2) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    3) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    4) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    5) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    6) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    7) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    8) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    9) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    10) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    11) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    12) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    13) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    14) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    15) Product Images from "Epithelial Cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is Crucial for Murine Asthma"

    Article Title: Epithelial Cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is Crucial for Murine Asthma

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1200461

    IL-25 can induce chemokine production by airway epithelial cells. The levels of CCL5 and CXCL1 in the culture supernatants and cell lysates of airway epithelial cells after stimulation with the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or
    Figure Legend Snippet: IL-25 can induce chemokine production by airway epithelial cells. The levels of CCL5 and CXCL1 in the culture supernatants and cell lysates of airway epithelial cells after stimulation with the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or

    Techniques Used:

    IL-25 can potently induce airway epithelial cell and eosinophil activation. A , B , Chemokine expression/production by MLE-12 cultured in the presence and absence of the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or 100-ng/ml rmIL-13 for 4 hours
    Figure Legend Snippet: IL-25 can potently induce airway epithelial cell and eosinophil activation. A , B , Chemokine expression/production by MLE-12 cultured in the presence and absence of the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or 100-ng/ml rmIL-13 for 4 hours

    Techniques Used: Activation Assay, Expressing, Cell Culture

    IL-25 enhances, but is not essential for, DC migration from the lungs to draining LNs.Mice were intranasally treated with FITC-OVA or PBS in the presence and absence of rmIL-25. At 12 hours ( A , B ) or 24 hours ( C ) after the inhalation, submaxillary and
    Figure Legend Snippet: IL-25 enhances, but is not essential for, DC migration from the lungs to draining LNs.Mice were intranasally treated with FITC-OVA or PBS in the presence and absence of rmIL-25. At 12 hours ( A , B ) or 24 hours ( C ) after the inhalation, submaxillary and

    Techniques Used: Migration, Mouse Assay

    16) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    17) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    18) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    19) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    20) Product Images from "Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *"

    Article Title: Estrogen Receptors Bind to and Activate the HOXC4/HoxC4 Promoter to Potentiate HoxC4-mediated Activation-induced Cytosine Deaminase Induction, Immunoglobulin Class Switch DNA Recombination, and Somatic Hypermutation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.169086

    HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4
    Figure Legend Snippet: HoxC4 deficiency impairs estrogen-mediated enhancement of AID expression and CSR to IgG1 and IgE, as induced by LPS and IL-4 or CD154 and IL-4. A , HoxC4 +/+ and HoxC4 −/− B cells were stimulated with LPS and rmIL-4 or with CD154 and rmIL-4

    Techniques Used: Expressing

    21) Product Images from "Epithelial Cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is Crucial for Murine Asthma 1"

    Article Title: Epithelial Cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is Crucial for Murine Asthma 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1200461

    IL-25 can induce chemokine production by airway epithelial cells. The levels of CCL5 and CXCL1 in the culture supernatants and cell lysates of airway epithelial cells after stimulation with the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or
    Figure Legend Snippet: IL-25 can induce chemokine production by airway epithelial cells. The levels of CCL5 and CXCL1 in the culture supernatants and cell lysates of airway epithelial cells after stimulation with the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or

    Techniques Used:

    IL-25 can potently induce airway epithelial cell and eosinophil activation. A , B , Chemokine expression/production by MLE-12 cultured in the presence and absence of the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or 100-ng/ml rmIL-13 for 4 hours
    Figure Legend Snippet: IL-25 can potently induce airway epithelial cell and eosinophil activation. A , B , Chemokine expression/production by MLE-12 cultured in the presence and absence of the indicated concentrations of rmIL-25, 100-ng/ml rmIL-4 or 100-ng/ml rmIL-13 for 4 hours

    Techniques Used: Activation Assay, Expressing, Cell Culture

    22) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    23) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

    24) Product Images from "Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions"

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2010.10.023

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each
    Figure Legend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Techniques Used: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ
    Figure Legend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Techniques Used: Labeling, In Situ

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    Article Title: Role of NADPH oxidase in interleukin-4-induced monocyte chemoattractant protein-1 expression in vascular endothelium
    Article Snippet: Primary human aortic endothelial cells (HAEC) were purchased from Cascade Biologics™ (Portland, OR) and cultured in medium 200 supplemented with low serum growth supplement (LSGS) in a 37°C, 5% CO2 /95% air, humidified cell culture incubator. .. Recombinant human and mouse IL-4 were purchased from R & D Systems Inc. (Minneapolis, MN) and dissolved in sterile phosphate buffered saline (PBS). ..

    Article Title: Trichostatin A Abrogates Airway Constriction, but Not Inflammation, in Murine and Human Asthma Models
    Article Snippet: .. We used the recombinant murine IL-4 and IL-6 included with the kits (R & D Systems, Minneapolis, MN) as control samples. .. Tissue lysates were prepared in lysis buffer consisting of 20 mM Tris HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton X-100, 1 μg/ml leupeptin, 2.5 mM sodium pyrophosphate, 1 mM Na3 VO4 , and 1 mM β-glycerophosphate.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Invariant NKT Cells Drive Hepatic Cytokinic Microenvironment Favoring Efficient Granuloma Formation and Early Control of Leishmania donovani Infection
    Article Snippet: All lysates were equalized with complement-free FCS to a 10% concentration, and tested whole and diluted 1∶2 in 10% complement-free FCS. .. The different cytokines were quantified using Duoset ELISA development systems for mouse IL-4, TNF-α, and IFN-γ (R & D Systems), according to the manufacturer’s instructions, except for the reveal step in which orthophenyl-dianizidine was used instead of tetramethylbenzidine. .. Absorbance was determined at 490 nm using a spectrophotometer, and the results were determined from a seven-point standard curve, and expressed as pg/mL/100 mg of tissue.

    Article Title: Secretoglobin 3A2 Exhibits Anti-Fibrotic Activity in Bleomycin-Induced Pulmonary Fibrosis Model Mice
    Article Snippet: Absorbance was measured at 560 nm in a microplate reader (SpectroMax Plus384, Molecular Devices, Sunnyvale, CA). .. The levels of mouse IL-4, IL-5, and IL-13 in BALF were determined by using ELISA kits from R & D systems (Mineapolis, MN) according to the manufacture’s protocol. .. qRT-PCR analysis The whole left lobe of each lung was used for total RNA isolation with TRIzol, digested with DNase I, and reverse transcribed with Superscript II reverse transcriptase.

    Expressing:

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions
    Article Snippet: .. To test the hypothesis that EndoG can localize to the nucleus in B cells undergoing CSR, we stimulated mouse B cells with LPS and rmIL-4, which induce CSR to IgG1, or with anti-IgD mAb-dextran and rmIL-4, which induce B cell proliferation but not CSR, and, after a 2-day culture, analyzed EndoG expression and localization by specific immunoblotting. .. EndoG localized to both the cytoplasm and the nucleus of B cells treated with anti-IgD mAb-dextran and rmIL-4, but its density at both locations increased significantly after stimulation with LPS and rmIL-4 ( ).

    Enzyme-linked Immunospot:

    Article Title: Inhibition of Neointima Formation through DNA Vaccination for Apolipoprotein(a): A New Therapeutic Strategy for Lipoprotein(a)
    Article Snippet: .. Enzyme-Linked ImmunoSpot (ELISpot) assay The ELISpot assay was carried out using the Mouse IFN-γ Development Module and the Mouse IL-4 Development Module for their respective targets (R & D Systems) according to the manufacturer's instructions. .. Briefly, 96-well plates for ELISpot (Millipore) were preincubated with anti-mouse IFN-γ or IL-4 antibodies overnight at 4°C and blocked with PBS containing 1% BSA and 5% sucrose for 2 hours at room temperature.

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    R&D Systems recombinant murine il 4
    Dex, but not TSA, inhibits antigen-induced increases of <t>IL-4</t> concentrations in BALF. Concentrations of ( A ) IL-4 and ( B ) IL-6 in BALF were measured from mice that were challenged with antigen or diluent (naive) and mice that received vehicle alone (DMSO),
    Recombinant Murine Il 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems recombinant mouse il 25
    Innate type 2 cells are induced in response to <t>IL-25,</t> α-Gal-Cer and sulfatides Obese WT mice were treated with PBS, IL-25, α-Gal-Cer or sulfatides on days 0, 2 and 4. The cellular composition of VAT was assessed by flow cytometry, including expression of ( A ) ILC2 (Lineage − ICOS + T1/ST2 + ), ( B ) type I NKT cells (TCRβ + NK1.1 + CD1d tetramers + ), ( C ) type II NKT cells (TCRβ + NK1.1 + CD1d tetramers − ). Weight ( D,G ) and ( E,H ) glucose tolerance was monitored in the groups treated with α-Gal-Cer, sulfatides or IL-25. ( F ) Visceral (VAT) and subcutaneous (SAT) adipose tissues were excised and weighed. Data are representative of n=2-6 (+/− SEM) from 2 independent experimental replicates (* P
    Recombinant Mouse Il 25, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems recombinant murine il 5
    GM-CSF, LPS and Siglec-F signaling enhance IL-33-induced secretion of IL-4 and IL-13 from eosinophils. (A) BMDE were cultured with <t>IL-5</t> and indicated concentrations of IL-33 and LPS. (B) BMDE were cultured in 10 ng/ml IL-5 and IL-33 in the presence or absence of 10 ng/ml GM-CSF. In addition, anti-Siglec-F, isotype control antibody or no antibodies were added to the culture. (C) BMDE were cultured in 10 ng/ml IL-5 and IL-33. In addition, the p38 kinase was blocked with 5 μM SB203580 and DMSO was used as solvent control. All supernatants were analyzed at 24 hours after onset of culture by IL-4 and IL-13 specific ELISAs. Bars show the mean + SD from one of two independent experiments with similar results (A; n = 4) the mean + SD from one of three independent experiments (B; n = 4) and mean + SEM pooled from two independent experiments (C; n = 8) *p
    Recombinant Murine Il 5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dex, but not TSA, inhibits antigen-induced increases of IL-4 concentrations in BALF. Concentrations of ( A ) IL-4 and ( B ) IL-6 in BALF were measured from mice that were challenged with antigen or diluent (naive) and mice that received vehicle alone (DMSO),

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Trichostatin A Abrogates Airway Constriction, but Not Inflammation, in Murine and Human Asthma Models

    doi: 10.1165/rcmb.2010-0276OC

    Figure Lengend Snippet: Dex, but not TSA, inhibits antigen-induced increases of IL-4 concentrations in BALF. Concentrations of ( A ) IL-4 and ( B ) IL-6 in BALF were measured from mice that were challenged with antigen or diluent (naive) and mice that received vehicle alone (DMSO),

    Article Snippet: We used the recombinant murine IL-4 and IL-6 included with the kits (R & D Systems, Minneapolis, MN) as control samples.

    Techniques: Mouse Assay

    Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Journal: Molecular immunology

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    doi: 10.1016/j.molimm.2010.10.023

    Figure Lengend Snippet: Sμ-Sγ1 DNA junctions in B cells. EndoG +/+ and EndoG −/− spleen B cells were stimulated by LPS and rmIL-4 for 4 days. Sμ-Sγ1 junction DNAs from stimulated cells were amplified, cloned, and sequenced. Each

    Article Snippet: To test the hypothesis that EndoG can localize to the nucleus in B cells undergoing CSR, we stimulated mouse B cells with LPS and rmIL-4, which induce CSR to IgG1, or with anti-IgD mAb-dextran and rmIL-4, which induce B cell proliferation but not CSR, and, after a 2-day culture, analyzed EndoG expression and localization by specific immunoblotting.

    Techniques: Amplification, Clone Assay

    DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Journal: Molecular immunology

    Article Title: Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions

    doi: 10.1016/j.molimm.2010.10.023

    Figure Lengend Snippet: DSBs are reduced in EndoG −/− B cells. EndoG +/+ and EndoG −/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ

    Article Snippet: To test the hypothesis that EndoG can localize to the nucleus in B cells undergoing CSR, we stimulated mouse B cells with LPS and rmIL-4, which induce CSR to IgG1, or with anti-IgD mAb-dextran and rmIL-4, which induce B cell proliferation but not CSR, and, after a 2-day culture, analyzed EndoG expression and localization by specific immunoblotting.

    Techniques: Labeling, In Situ

    Innate type 2 cells are induced in response to IL-25, α-Gal-Cer and sulfatides Obese WT mice were treated with PBS, IL-25, α-Gal-Cer or sulfatides on days 0, 2 and 4. The cellular composition of VAT was assessed by flow cytometry, including expression of ( A ) ILC2 (Lineage − ICOS + T1/ST2 + ), ( B ) type I NKT cells (TCRβ + NK1.1 + CD1d tetramers + ), ( C ) type II NKT cells (TCRβ + NK1.1 + CD1d tetramers − ). Weight ( D,G ) and ( E,H ) glucose tolerance was monitored in the groups treated with α-Gal-Cer, sulfatides or IL-25. ( F ) Visceral (VAT) and subcutaneous (SAT) adipose tissues were excised and weighed. Data are representative of n=2-6 (+/− SEM) from 2 independent experimental replicates (* P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IL-25 elicits innate lymphoid type 2 and type II natural killer T cells that regulate obesity in mice1

    doi: 10.4049/jimmunol.1301176

    Figure Lengend Snippet: Innate type 2 cells are induced in response to IL-25, α-Gal-Cer and sulfatides Obese WT mice were treated with PBS, IL-25, α-Gal-Cer or sulfatides on days 0, 2 and 4. The cellular composition of VAT was assessed by flow cytometry, including expression of ( A ) ILC2 (Lineage − ICOS + T1/ST2 + ), ( B ) type I NKT cells (TCRβ + NK1.1 + CD1d tetramers + ), ( C ) type II NKT cells (TCRβ + NK1.1 + CD1d tetramers − ). Weight ( D,G ) and ( E,H ) glucose tolerance was monitored in the groups treated with α-Gal-Cer, sulfatides or IL-25. ( F ) Visceral (VAT) and subcutaneous (SAT) adipose tissues were excised and weighed. Data are representative of n=2-6 (+/− SEM) from 2 independent experimental replicates (* P

    Article Snippet: After 8 weeks on HFD mice were injected on days 0, 2 and 4 with 0.4 μg recombinant mouse IL-25 (R & D Systems, Abingdon, UK), 20 μg α-Galactosylceraminde (α-Gal-Cer; Avanti Polar Lipids Inc., AL, USA) or 20 μg sulfatides (Matreya LLC, PA, USA) and weight monitored for 6 days.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Expressing

    GM-CSF, LPS and Siglec-F signaling enhance IL-33-induced secretion of IL-4 and IL-13 from eosinophils. (A) BMDE were cultured with IL-5 and indicated concentrations of IL-33 and LPS. (B) BMDE were cultured in 10 ng/ml IL-5 and IL-33 in the presence or absence of 10 ng/ml GM-CSF. In addition, anti-Siglec-F, isotype control antibody or no antibodies were added to the culture. (C) BMDE were cultured in 10 ng/ml IL-5 and IL-33. In addition, the p38 kinase was blocked with 5 μM SB203580 and DMSO was used as solvent control. All supernatants were analyzed at 24 hours after onset of culture by IL-4 and IL-13 specific ELISAs. Bars show the mean + SD from one of two independent experiments with similar results (A; n = 4) the mean + SD from one of three independent experiments (B; n = 4) and mean + SEM pooled from two independent experiments (C; n = 8) *p

    Journal: PLoS ONE

    Article Title: IL-33-Induced Cytokine Secretion and Survival of Mouse Eosinophils Is Promoted by Autocrine GM-CSF

    doi: 10.1371/journal.pone.0163751

    Figure Lengend Snippet: GM-CSF, LPS and Siglec-F signaling enhance IL-33-induced secretion of IL-4 and IL-13 from eosinophils. (A) BMDE were cultured with IL-5 and indicated concentrations of IL-33 and LPS. (B) BMDE were cultured in 10 ng/ml IL-5 and IL-33 in the presence or absence of 10 ng/ml GM-CSF. In addition, anti-Siglec-F, isotype control antibody or no antibodies were added to the culture. (C) BMDE were cultured in 10 ng/ml IL-5 and IL-33. In addition, the p38 kinase was blocked with 5 μM SB203580 and DMSO was used as solvent control. All supernatants were analyzed at 24 hours after onset of culture by IL-4 and IL-13 specific ELISAs. Bars show the mean + SD from one of two independent experiments with similar results (A; n = 4) the mean + SD from one of three independent experiments (B; n = 4) and mean + SEM pooled from two independent experiments (C; n = 8) *p

    Article Snippet: From day 4 on cells were kept in BM-medium supplemented with 10 ng/ml recombinant murine IL-5 (R & D Systems, Minneapolis, MN).

    Techniques: Cell Culture

    IL-33 mediates eosinophil survival that depends on ST2 and MyD88. BMDE from wild-type (WT), ST2-deficient (ST2 -/- ) and MyD88-deficient (MyD88 -/- ) mice were cultured in presence of 10 ng/ml IL-5 or IL-33 or were left untreated for 72 hours. Cells were harvested and analyzed by flow cytometry to detect apoptotic cells. (A) Dot plots show representative AnnexinV/PI stainings for each experimental group. (B) Bars show the mean + SEM of apoptotic cells from indicated mice. Data are pooled from two independent experiments (n = 5–6; *** p

    Journal: PLoS ONE

    Article Title: IL-33-Induced Cytokine Secretion and Survival of Mouse Eosinophils Is Promoted by Autocrine GM-CSF

    doi: 10.1371/journal.pone.0163751

    Figure Lengend Snippet: IL-33 mediates eosinophil survival that depends on ST2 and MyD88. BMDE from wild-type (WT), ST2-deficient (ST2 -/- ) and MyD88-deficient (MyD88 -/- ) mice were cultured in presence of 10 ng/ml IL-5 or IL-33 or were left untreated for 72 hours. Cells were harvested and analyzed by flow cytometry to detect apoptotic cells. (A) Dot plots show representative AnnexinV/PI stainings for each experimental group. (B) Bars show the mean + SEM of apoptotic cells from indicated mice. Data are pooled from two independent experiments (n = 5–6; *** p

    Article Snippet: From day 4 on cells were kept in BM-medium supplemented with 10 ng/ml recombinant murine IL-5 (R & D Systems, Minneapolis, MN).

    Techniques: Mouse Assay, Cell Culture, Flow Cytometry, Cytometry

    IL-33 mediates eosinophil survival via autocrine GM-CSF production. (A) BMDE were cultured in medium containing 10 ng/ml IL-5 and indicated concentrations of IL-33 and LPS for 24 hours. Supernatants were analyzed by GM-CSF-specific ELISA. Bar graph shows the mean + SD from one of two independent experiments (n = 4;). (B) BMDE were stimulated with the indicated cytokines in the absence (black bars) or presence of a isotype control antibody (dark grey bars) or GM-CSF receptor blocking antibody (light grey bars). After 72 hours cells were analyzed by flow cytometry to detect Annexin V + cells. Bars show the mean + SEM of apoptotic cells from three independent experiments (n = 6; ** p

    Journal: PLoS ONE

    Article Title: IL-33-Induced Cytokine Secretion and Survival of Mouse Eosinophils Is Promoted by Autocrine GM-CSF

    doi: 10.1371/journal.pone.0163751

    Figure Lengend Snippet: IL-33 mediates eosinophil survival via autocrine GM-CSF production. (A) BMDE were cultured in medium containing 10 ng/ml IL-5 and indicated concentrations of IL-33 and LPS for 24 hours. Supernatants were analyzed by GM-CSF-specific ELISA. Bar graph shows the mean + SD from one of two independent experiments (n = 4;). (B) BMDE were stimulated with the indicated cytokines in the absence (black bars) or presence of a isotype control antibody (dark grey bars) or GM-CSF receptor blocking antibody (light grey bars). After 72 hours cells were analyzed by flow cytometry to detect Annexin V + cells. Bars show the mean + SEM of apoptotic cells from three independent experiments (n = 6; ** p

    Article Snippet: From day 4 on cells were kept in BM-medium supplemented with 10 ng/ml recombinant murine IL-5 (R & D Systems, Minneapolis, MN).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Blocking Assay, Flow Cytometry, Cytometry

    IL-33 activated eosinophils mediate alternative activation of macrophages. (A) and (B) BMDM generated from wild-type BALB/c mice were co-cultured with BMDE from either wild-type (black bars) or IL-4/IL-13-deficient BALB/c mice (grey bars) for 24 hours in the presence of the indicated cytokines. Quantitative RT- PCR was performed to analyze Relm-α and Arginase-1 expression as established markers for alternative activation of macrophages. Data are presented as normalized expression to PBGD. Bars show the mean + SD (n = 3) from one of three independent experiments with similar results. (C) and (D) only supernatants of IL-5+IL-33+GM-CSF stimulated BMDE from WT (black bars) or IL-4/IL-13-deficient mice (grey bars) were added to BMDM for 24 hours before quantitative RT-PCR was performed. Bars show the mean + SD (n = 3) from one experiment.

    Journal: PLoS ONE

    Article Title: IL-33-Induced Cytokine Secretion and Survival of Mouse Eosinophils Is Promoted by Autocrine GM-CSF

    doi: 10.1371/journal.pone.0163751

    Figure Lengend Snippet: IL-33 activated eosinophils mediate alternative activation of macrophages. (A) and (B) BMDM generated from wild-type BALB/c mice were co-cultured with BMDE from either wild-type (black bars) or IL-4/IL-13-deficient BALB/c mice (grey bars) for 24 hours in the presence of the indicated cytokines. Quantitative RT- PCR was performed to analyze Relm-α and Arginase-1 expression as established markers for alternative activation of macrophages. Data are presented as normalized expression to PBGD. Bars show the mean + SD (n = 3) from one of three independent experiments with similar results. (C) and (D) only supernatants of IL-5+IL-33+GM-CSF stimulated BMDE from WT (black bars) or IL-4/IL-13-deficient mice (grey bars) were added to BMDM for 24 hours before quantitative RT-PCR was performed. Bars show the mean + SD (n = 3) from one experiment.

    Article Snippet: From day 4 on cells were kept in BM-medium supplemented with 10 ng/ml recombinant murine IL-5 (R & D Systems, Minneapolis, MN).

    Techniques: Activation Assay, Generated, Mouse Assay, Cell Culture, Quantitative RT-PCR, Expressing