rlt lysis buffer provided in the rneasy kit qiagen hilden germany  (Qiagen)

 
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    Qiagen rlt lysis buffer provided in the rneasy kit qiagen hilden germany
    Rlt Lysis Buffer Provided In The Rneasy Kit Qiagen Hilden Germany, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rlt lysis buffer provided in the rneasy kit qiagen hilden germany/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rlt lysis buffer provided in the rneasy kit qiagen hilden germany - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Molecular Analysis of a Short-term Model of β-Glucans-Trained Immunity Highlights the Accessory Contribution of GM-CSF in Priming Mouse Macrophages Response
    Article Snippet: .. Microarray Analysis After stimulation with Sc BG compounds and then with ultraPure LPS, BMDM were lysed in Buffer RLT (Qiagen, Hilden, Germany) and was subjected to RNA extraction using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. RNA was quantified with a NanoDrop® 1000 spectrophotometer and NanoDrop 1000.3.7 software.

    Isolation:

    Article Title: Pneumococcal quorum sensing drives an asymmetric owner-intruder competitive strategy during carriage via the competence regulon
    Article Snippet: .. To obtain host RNA, nasal lavages with RLT lysis buffer (Qiagen) were performed and RNA isolated with the RNeasy minikit (Qiagen) as previously described . cDNA was reverse transcribed using a high-capacity cDNA kit (Applied Biosystems) following the manufacturer’s instructions. .. Real-time, quantitative reverse transcriptase PCR was carried out with Power SYBR Green Master Mix (Applied Biosystems) using 10–50 ng cDNA and 0.5 μM primers per reaction.

    Centrifugation:

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
    Article Snippet: .. Treatment with Buffer RLT additionally enriches fungal RNA After testing the enrichment of cells and of DNA from the pathogenic fungus with three different methods, and with the addition of a centrifugation step, we evaluated whether RNA was also enriched together with DNA. .. Therefore, the nucleic acids obtained from the differently treated mixes after extraction by means of the RiboPure Yeast Kit, were further treated with DNase to obtain pure RNA, and quantitative reverse transcription PCR (RT-qPCR) was carried out.

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
    Article Snippet: .. In addition, using qPCR and RT-qPCR, we demonstrated that after cell lysis with Buffer RLT, both fungal DNA and RNA can be further enriched by means of a centrifugation step that effectively reduced human nucleic acids. .. However, we observed that pellets of fungal cells were not strongly attached at the bottom of the tube, which may be due to the presence of large amounts of (human) DNA in the supernatant, which may increase viscosity, and interfere with the centrifugation process.

    Cell Culture:

    Article Title: Clearance of Pneumococcal Colonization in Infants Is Delayed through Altered Macrophage Trafficking
    Article Snippet: .. qRT-PCR RNA was obtained from URT epithelium by lavage with RLT buffer (Qiagen) with 1% β-mercaptoethanol, or from cultured peritoneal macrophages by lysing cells in RLT buffer with 1% β-mercaptoethanol and frozen at -80°C until used. .. An RNeasy kit (Qiagen) was used to isolate RNA, and cDNA reverse transcribed by the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). qRT-PCR reactions were performed with Sybr Green (Applied Biosystems) with 10 ng cDNA and 0.5 μM primers.

    Real-time Polymerase Chain Reaction:

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
    Article Snippet: .. In addition, using qPCR and RT-qPCR, we demonstrated that after cell lysis with Buffer RLT, both fungal DNA and RNA can be further enriched by means of a centrifugation step that effectively reduced human nucleic acids. .. However, we observed that pellets of fungal cells were not strongly attached at the bottom of the tube, which may be due to the presence of large amounts of (human) DNA in the supernatant, which may increase viscosity, and interfere with the centrifugation process.

    Microarray:

    Article Title: Molecular Analysis of a Short-term Model of β-Glucans-Trained Immunity Highlights the Accessory Contribution of GM-CSF in Priming Mouse Macrophages Response
    Article Snippet: .. Microarray Analysis After stimulation with Sc BG compounds and then with ultraPure LPS, BMDM were lysed in Buffer RLT (Qiagen, Hilden, Germany) and was subjected to RNA extraction using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. RNA was quantified with a NanoDrop® 1000 spectrophotometer and NanoDrop 1000.3.7 software.

    other:

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
    Article Snippet: We concluded that enrichment with Buffer RLT confers high-quality RNA which can be used in transcriptomic analysis.

    Quantitative RT-PCR:

    Article Title: Clearance of Pneumococcal Colonization in Infants Is Delayed through Altered Macrophage Trafficking
    Article Snippet: .. qRT-PCR RNA was obtained from URT epithelium by lavage with RLT buffer (Qiagen) with 1% β-mercaptoethanol, or from cultured peritoneal macrophages by lysing cells in RLT buffer with 1% β-mercaptoethanol and frozen at -80°C until used. .. An RNeasy kit (Qiagen) was used to isolate RNA, and cDNA reverse transcribed by the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). qRT-PCR reactions were performed with Sybr Green (Applied Biosystems) with 10 ng cDNA and 0.5 μM primers.

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
    Article Snippet: .. In addition, using qPCR and RT-qPCR, we demonstrated that after cell lysis with Buffer RLT, both fungal DNA and RNA can be further enriched by means of a centrifugation step that effectively reduced human nucleic acids. .. However, we observed that pellets of fungal cells were not strongly attached at the bottom of the tube, which may be due to the presence of large amounts of (human) DNA in the supernatant, which may increase viscosity, and interfere with the centrifugation process.

    Lysis:

    Article Title: Pneumococcal quorum sensing drives an asymmetric owner-intruder competitive strategy during carriage via the competence regulon
    Article Snippet: .. To obtain host RNA, nasal lavages with RLT lysis buffer (Qiagen) were performed and RNA isolated with the RNeasy minikit (Qiagen) as previously described . cDNA was reverse transcribed using a high-capacity cDNA kit (Applied Biosystems) following the manufacturer’s instructions. .. Real-time, quantitative reverse transcriptase PCR was carried out with Power SYBR Green Master Mix (Applied Biosystems) using 10–50 ng cDNA and 0.5 μM primers per reaction.

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions
    Article Snippet: .. In addition, using qPCR and RT-qPCR, we demonstrated that after cell lysis with Buffer RLT, both fungal DNA and RNA can be further enriched by means of a centrifugation step that effectively reduced human nucleic acids. .. However, we observed that pellets of fungal cells were not strongly attached at the bottom of the tube, which may be due to the presence of large amounts of (human) DNA in the supernatant, which may increase viscosity, and interfere with the centrifugation process.

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    Qiagen rlt lysis buffer provided in the rneasy kit qiagen hilden germany
    Rlt Lysis Buffer Provided In The Rneasy Kit Qiagen Hilden Germany, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rlt lysis buffer provided in the rneasy kit qiagen hilden germany/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rlt lysis buffer provided in the rneasy kit qiagen hilden germany - by Bioz Stars, 2020-05
    99/100 stars
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