rlt buffer from rneasy mini kit qiagen  (Qiagen)


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    Qiagen rlt buffer from rneasy mini kit qiagen
    Rlt Buffer From Rneasy Mini Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rlt buffer from rneasy mini kit qiagen - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Requirement for Zebrafish Ataxin-7 in Differentiation of Photoreceptors and Cerebellar Neurons
    Article Snippet: RNAs were isolated using the RNeasy mini Kit (Qiagen, Germantown, MD, USA) and then reverse transcribed as cDNA using the SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. A cDNA fragment of zebrafish atxn7 encompassing exons 3 to 5 was PCR amplified using the zatxn7 -forw ( 5′-GGCCTTCCAAGCACATTAC-3′ ) and zatxn7 -rev ( 5′-GTCATATCCATAACCCCAC-3′ ) primers.

    Mass Spectrometry:

    Article Title: Genetic PEGylation
    Article Snippet: The PEG–AF–pdCpA products were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI–TOF–MS; Voyager DE-PRO, Applied Biosystems, Foster City, CA, USA): PEG16–AF–pdCpA, calculated 1543.61 for (M–H)– , found 1544.09; PEG24–AF–pdCpA, calculated 1895.82 for (M–H)– , found 1896.41. .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Synthesized:

    Article Title: Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis
    Article Snippet: Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. .. In the case of terminally differentiated neuronal cultures, RNA extractions were performed at 59 days in vitro . cDNA was synthesized from 2 μg of RNA using Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and oligo-dT primers.

    Article Title: The Dermatan Sulfate Proteoglycan Decorin Modulates ?2?1 Integrin and the Vimentin Intermediate Filament System during Collagen Synthesis
    Article Snippet: .. Total RNA was extracted from cells at day 3, 6 and 14 using RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 2 µg of total RNA using Omniscript RT Kit (Qiagen). mRNA levels of target genes were quantified by Real-Time RT-PCR using an ABI PRISM 7500 Sequence Detector (Applied Biosystems) (core unit IZKF Münster) and MESA GREEN (Eurogentec, Köln, Germany). ..

    Article Title: Increased Expression of the Very Low-Density Lipoprotein Receptor Mediates Lipid Accumulation in Clear-Cell Renal Cell Carcinoma
    Article Snippet: .. Analysis of mRNA and protein expression Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) and cRNA was synthesized using the cDNA archive kit (Applied Biosciences). ..

    Quantitative RT-PCR:

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times. .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) using SYBR Green I dye (LightCycler FastStart DNA Master SYBR Green I, Roche Diagnostics, Mannheim, Germany) and the primer sets listed in Table was conducted to measure mRNA expression levels, as described previously [ ].

    Article Title: The Dermatan Sulfate Proteoglycan Decorin Modulates ?2?1 Integrin and the Vimentin Intermediate Filament System during Collagen Synthesis
    Article Snippet: .. Total RNA was extracted from cells at day 3, 6 and 14 using RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 2 µg of total RNA using Omniscript RT Kit (Qiagen). mRNA levels of target genes were quantified by Real-Time RT-PCR using an ABI PRISM 7500 Sequence Detector (Applied Biosystems) (core unit IZKF Münster) and MESA GREEN (Eurogentec, Köln, Germany). ..

    Article Title: Orphan Toxin OrtT (YdcX) of Escherichia coli Reduces Growth during the Stringent Response
    Article Snippet: .. 4.11. qRT-PCR Total RNA was isolated as described previously [ ] using Qiagen RNeasy mini kit (Valencia, CA, USA). ..

    SYBR Green Assay:

    Article Title: Cohesin Is Required for Activation of MYC by Estradiol
    Article Snippet: RNA was processed using the Qiagen RNeasy mini kit with DNAse treatment (Qiagen). cDNA was transcribed using the Superscript III first strand synthesis system, according to the manufacturer’s instructions (Invitrogen). .. Real-time quantitative PCR was performed using Platinum® SYBR® Green qPCR SuperMix-UDG with ROX (Invitrogen) on an ABI 7300 Real Time PCR System (Applied Biosystems). qBase Plus (Biogazelle) was used to quantify the transcript levels of each gene, relative to endogenous controls for each sample.

    Article Title: Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
    Article Snippet: Quantitative Real-Time PCR (qPCR) Total amount of RNA was extracted using RNeasy Mini Kit (Qiagen AG, Hombrechtikon, Switzerland) and the reverse transcription reaction was performed with 0.5 µg RNA in a 20 µl reaction using random primers (Invitrogen) with the Superscript III Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The qPCR reaction was done on a LightCycler 480 system (Roche Diagnostics, Rotkreuz, Switzerland) utilizing the hot-start SYBR green method with the following parameters: preheating for 10 min at 95°C, followed by 45 cycles of denaturation for 5 sec at 95°C, annealing for 10 sec at 60°C and extension for 6 sec at 72°C.

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times. .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) using SYBR Green I dye (LightCycler FastStart DNA Master SYBR Green I, Roche Diagnostics, Mannheim, Germany) and the primer sets listed in Table was conducted to measure mRNA expression levels, as described previously [ ].

    Article Title: Pgc-1? Overexpression Downregulates Pitx3 and Increases Susceptibility to MPTP Toxicity Associated with Decreased Bdnf
    Article Snippet: Expression Analysis After dissection as before, RNA was extracted from the striata or SN using the RNeasy mini kit (Qiagen). .. The final cDNA reaction was diluted seven-fold and 5 ul of the reaction was used in duplicate 15 ul SYBR-Green (Applied Biosystems) PCR reactions with primers specific to the target gene.

    Article Title: Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis
    Article Snippet: Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. .. Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions.

    Microarray:

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: Paragraph title: Microarray Analysis ... Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA.

    Incubation:

    Article Title: P2X7 Receptor Activation Impairs Exogenous MHC Class I Oligopeptides Presentation in Antigen Presenting Cells
    Article Snippet: Quantitative Reverse Transcriptase–polymerase Chain Reaction (RT-PCR) Analysis BMDM in 12 well plates were incubated 30 min in the presence or absence of ATP (5 mM), then washed with PBS, followed by incubation for 2 h with 1 µg/ml LPS or vehicle. .. After LPS stimulation, total RNA was extracted using the RNeasy Mini kit (Qiagen), followed by reverse transcription using iScript cDNA Synthesis (Bio-Rad) with oligo(dT).

    Article Title: Genetic PEGylation
    Article Snippet: .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. PEG16–, PEG24–, and PEG48–AF–tRNA were purified by reversed-phase HPLC (Applied Biosystems POROS R2/10; 10 µm, 4.6 mm×100 mm) at a flow rate of 1.0 mL/min, with a linear gradient of 0%–100% acetonitrile in 0.1% triethylammonium acetate for 20 min.

    Activity Assay:

    Article Title: Requirement for Zebrafish Ataxin-7 in Differentiation of Photoreceptors and Cerebellar Neurons
    Article Snippet: Paragraph title: RT-PCR Analysis of MOzatxnSPL Splice-blocking Activity ... RNAs were isolated using the RNeasy mini Kit (Qiagen, Germantown, MD, USA) and then reverse transcribed as cDNA using the SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: Protein Secretion Is Required for Pregnancy-Associated Plasma Protein-A to Promote Lung Cancer Growth In Vivo
    Article Snippet: .. Expression analysis of cancer cell lines by real-time PCR The RNeasy Mini kit (Qiagen, Valencia, CA) was used to prepare total RNA from cell culture. .. Total RNA was treated with DNase according to the manufacturer's instructions (Qiagen).

    Expressing:

    Article Title: Cohesin Is Required for Activation of MYC by Estradiol
    Article Snippet: Paragraph title: Gene Expression Analysis ... RNA was processed using the Qiagen RNeasy mini kit with DNAse treatment (Qiagen). cDNA was transcribed using the Superscript III first strand synthesis system, according to the manufacturer’s instructions (Invitrogen).

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times. .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) using SYBR Green I dye (LightCycler FastStart DNA Master SYBR Green I, Roche Diagnostics, Mannheim, Germany) and the primer sets listed in Table was conducted to measure mRNA expression levels, as described previously [ ].

    Article Title: Pgc-1? Overexpression Downregulates Pitx3 and Increases Susceptibility to MPTP Toxicity Associated with Decreased Bdnf
    Article Snippet: .. Expression Analysis After dissection as before, RNA was extracted from the striata or SN using the RNeasy mini kit (Qiagen). .. After DNase treatment the RNA was purified using the RNeasy MinElute Clean-up kit (Qiagen) and used as a template for cDNA synthesis with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Article Title: Protein Secretion Is Required for Pregnancy-Associated Plasma Protein-A to Promote Lung Cancer Growth In Vivo
    Article Snippet: .. Expression analysis of cancer cell lines by real-time PCR The RNeasy Mini kit (Qiagen, Valencia, CA) was used to prepare total RNA from cell culture. .. Total RNA was treated with DNase according to the manufacturer's instructions (Qiagen).

    Article Title: P2X7 Receptor Activation Impairs Exogenous MHC Class I Oligopeptides Presentation in Antigen Presenting Cells
    Article Snippet: After LPS stimulation, total RNA was extracted using the RNeasy Mini kit (Qiagen), followed by reverse transcription using iScript cDNA Synthesis (Bio-Rad) with oligo(dT). .. Relative gene expression levels were calculated using the 2−ΔΔCt method normalized to GAPDH expression level for each treatment and the fold increase in gene expression was relative to resting (non LPS) condition.

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA. .. Preparation of cDNA was performed according to Agilent's one-color microarray-based gene expression analysis instructions.

    Article Title: The Dermatan Sulfate Proteoglycan Decorin Modulates ?2?1 Integrin and the Vimentin Intermediate Filament System during Collagen Synthesis
    Article Snippet: mRNA Extraction and Quantitative Real-time PCR For gene expression analysis cells were seeded in 24-well plates (Greiner, Frickenhausen, Germany). .. Total RNA was extracted from cells at day 3, 6 and 14 using RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 2 µg of total RNA using Omniscript RT Kit (Qiagen). mRNA levels of target genes were quantified by Real-Time RT-PCR using an ABI PRISM 7500 Sequence Detector (Applied Biosystems) (core unit IZKF Münster) and MESA GREEN (Eurogentec, Köln, Germany).

    Article Title: Increased Expression of the Very Low-Density Lipoprotein Receptor Mediates Lipid Accumulation in Clear-Cell Renal Cell Carcinoma
    Article Snippet: .. Analysis of mRNA and protein expression Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) and cRNA was synthesized using the cDNA archive kit (Applied Biosciences). ..

    Hybridization:

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA. .. Microarray hybridization was conducted on the EmbryoGENE bovine microarray .

    High Performance Liquid Chromatography:

    Article Title: Genetic PEGylation
    Article Snippet: After incubation at 37°C for 3 h, PEG–AF–pdCpA was purified by reversed-phase HPLC (Waters XTerra C18; 2.5 µm, 4.6 mm×20 mm) at a flow rate of 1.5 mL/min, with a linear gradient of 0%–100% acetonitrile in 0.1% trifluoroacetic acid (TFA) for 10 min. .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Flow Cytometry:

    Article Title: Genetic PEGylation
    Article Snippet: After incubation at 37°C for 3 h, PEG–AF–pdCpA was purified by reversed-phase HPLC (Waters XTerra C18; 2.5 µm, 4.6 mm×20 mm) at a flow rate of 1.5 mL/min, with a linear gradient of 0%–100% acetonitrile in 0.1% trifluoroacetic acid (TFA) for 10 min. .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Ligation:

    Article Title: Genetic PEGylation
    Article Snippet: The ligation reaction mixture (20 µL) contained tRNA lacking the 3′ dinucleotide (0.5 nmol), PEG–AF–pdCpA in water (4.4 nmol, 2 µL), HEPES-Na (55 mM, pH 7.5), ATP (1 mM), MgCl2 (15 mM), DTT (3.3 mM), BSA (20 µg/mL), and T4 RNA ligase (30 U). .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Dissection:

    Article Title: Pgc-1? Overexpression Downregulates Pitx3 and Increases Susceptibility to MPTP Toxicity Associated with Decreased Bdnf
    Article Snippet: .. Expression Analysis After dissection as before, RNA was extracted from the striata or SN using the RNeasy mini kit (Qiagen). .. After DNase treatment the RNA was purified using the RNeasy MinElute Clean-up kit (Qiagen) and used as a template for cDNA synthesis with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Infection:

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Additionally, we checked one defensin gene related to tick innate immunity in the midgut [ ], namely, H. longicornis midgut defensin (Hlgut-defensin ) [GenBank: EF432731] to judge the microbial contamination or infection in AE ticks since the transcription of this gene is drastically induced by lipopolysaccharide (LPS) [ ]. .. Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times.

    Inhibition:

    Article Title: Requirement for Zebrafish Ataxin-7 in Differentiation of Photoreceptors and Cerebellar Neurons
    Article Snippet: RT-PCR Analysis of MOzatxnSPL Splice-blocking Activity To check the efficiency of MOzatxn7SPL -mediated splice inhibition, a reverse transcription polymerase chain reaction (RT-PCR) was performed using RNAs, which were extracted from embryos that had received 0.3, 0.6, and 1 pmol MOzatxn7SPL . .. RNAs were isolated using the RNeasy mini Kit (Qiagen, Germantown, MD, USA) and then reverse transcribed as cDNA using the SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.

    Sequencing:

    Article Title: Transcriptome Pyrosequencing of the Parasitoid Wasp Cotesia vestalis: Genes Involved in the Antennal Odorant-Sensory System
    Article Snippet: .. RNA Preparation, cDNA Library Construction and 454 Sequencing Total RNA from 6 unit samples (a total of 360 female antennae) was isolated using a Qiagen RNeasy Mini Kit and an RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Total RNA (4.5 µg) was then taken through mRNA purification using a RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions (yielding 600 ng mRNA).

    Article Title: The Dermatan Sulfate Proteoglycan Decorin Modulates ?2?1 Integrin and the Vimentin Intermediate Filament System during Collagen Synthesis
    Article Snippet: .. Total RNA was extracted from cells at day 3, 6 and 14 using RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 2 µg of total RNA using Omniscript RT Kit (Qiagen). mRNA levels of target genes were quantified by Real-Time RT-PCR using an ABI PRISM 7500 Sequence Detector (Applied Biosystems) (core unit IZKF Münster) and MESA GREEN (Eurogentec, Köln, Germany). ..

    RNA Sequencing Assay:

    Article Title: Transcriptome Pyrosequencing of the Parasitoid Wasp Cotesia vestalis: Genes Involved in the Antennal Odorant-Sensory System
    Article Snippet: RNA Preparation, cDNA Library Construction and 454 Sequencing Total RNA from 6 unit samples (a total of 360 female antennae) was isolated using a Qiagen RNeasy Mini Kit and an RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Total RNA (4.5 µg) was then taken through mRNA purification using a RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions (yielding 600 ng mRNA).

    Multiple Displacement Amplification:

    Article Title: Cohesin Is Required for Activation of MYC by Estradiol
    Article Snippet: RNA was processed using the Qiagen RNeasy mini kit with DNAse treatment (Qiagen). cDNA was transcribed using the Superscript III first strand synthesis system, according to the manufacturer’s instructions (Invitrogen). .. For experiments using T47D or MDA-MB-231 cells Cyclophilin was used in addition to GAPDH and RPL13a .

    Isolation:

    Article Title: Cohesin Is Required for Activation of MYC by Estradiol
    Article Snippet: Gene Expression Analysis Total RNA was isolated from treated cells using Trizol Reagent (Invitrogen), according to the manufacturer’s instructions. .. RNA was processed using the Qiagen RNeasy mini kit with DNAse treatment (Qiagen). cDNA was transcribed using the Superscript III first strand synthesis system, according to the manufacturer’s instructions (Invitrogen).

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times. .. Total RNA was isolated from the homogenates according to the manufacturer’s instructions and used to synthesize cDNA (RNA PCR Kit Ver 3.0, Takara Bio INC., Shiga, Japan).

    Article Title: Transcriptome Pyrosequencing of the Parasitoid Wasp Cotesia vestalis: Genes Involved in the Antennal Odorant-Sensory System
    Article Snippet: .. RNA Preparation, cDNA Library Construction and 454 Sequencing Total RNA from 6 unit samples (a total of 360 female antennae) was isolated using a Qiagen RNeasy Mini Kit and an RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Total RNA (4.5 µg) was then taken through mRNA purification using a RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions (yielding 600 ng mRNA).

    Article Title: Requirement for Zebrafish Ataxin-7 in Differentiation of Photoreceptors and Cerebellar Neurons
    Article Snippet: .. RNAs were isolated using the RNeasy mini Kit (Qiagen, Germantown, MD, USA) and then reverse transcribed as cDNA using the SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. A cDNA fragment of zebrafish atxn7 encompassing exons 3 to 5 was PCR amplified using the zatxn7 -forw ( 5′-GGCCTTCCAAGCACATTAC-3′ ) and zatxn7 -rev ( 5′-GTCATATCCATAACCCCAC-3′ ) primers.

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: .. Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA. .. RNA quality was then assessed using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) and RNA 6000 Nano Labchip kit (Agilent Technologies) according to manufacturer's instructions.

    Article Title: Orphan Toxin OrtT (YdcX) of Escherichia coli Reduces Growth during the Stringent Response
    Article Snippet: .. 4.11. qRT-PCR Total RNA was isolated as described previously [ ] using Qiagen RNeasy mini kit (Valencia, CA, USA). ..

    Article Title: Increased Expression of the Very Low-Density Lipoprotein Receptor Mediates Lipid Accumulation in Clear-Cell Renal Cell Carcinoma
    Article Snippet: .. Analysis of mRNA and protein expression Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) and cRNA was synthesized using the cDNA archive kit (Applied Biosciences). ..

    Polymerase Chain Reaction:

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times. .. Total RNA was isolated from the homogenates according to the manufacturer’s instructions and used to synthesize cDNA (RNA PCR Kit Ver 3.0, Takara Bio INC., Shiga, Japan).

    Article Title: Pgc-1? Overexpression Downregulates Pitx3 and Increases Susceptibility to MPTP Toxicity Associated with Decreased Bdnf
    Article Snippet: Expression Analysis After dissection as before, RNA was extracted from the striata or SN using the RNeasy mini kit (Qiagen). .. The final cDNA reaction was diluted seven-fold and 5 ul of the reaction was used in duplicate 15 ul SYBR-Green (Applied Biosystems) PCR reactions with primers specific to the target gene.

    Article Title: Requirement for Zebrafish Ataxin-7 in Differentiation of Photoreceptors and Cerebellar Neurons
    Article Snippet: RNAs were isolated using the RNeasy mini Kit (Qiagen, Germantown, MD, USA) and then reverse transcribed as cDNA using the SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. .. A cDNA fragment of zebrafish atxn7 encompassing exons 3 to 5 was PCR amplified using the zatxn7 -forw ( 5′-GGCCTTCCAAGCACATTAC-3′ ) and zatxn7 -rev ( 5′-GTCATATCCATAACCCCAC-3′ ) primers.

    Article Title: Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis
    Article Snippet: Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. .. Polymerase chain reaction (PCR) was carried out using Taq polymerase (Invitrogen).

    Size-exclusion Chromatography:

    Article Title: Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
    Article Snippet: Quantitative Real-Time PCR (qPCR) Total amount of RNA was extracted using RNeasy Mini Kit (Qiagen AG, Hombrechtikon, Switzerland) and the reverse transcription reaction was performed with 0.5 µg RNA in a 20 µl reaction using random primers (Invitrogen) with the Superscript III Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The qPCR reaction was done on a LightCycler 480 system (Roche Diagnostics, Rotkreuz, Switzerland) utilizing the hot-start SYBR green method with the following parameters: preheating for 10 min at 95°C, followed by 45 cycles of denaturation for 5 sec at 95°C, annealing for 10 sec at 60°C and extension for 6 sec at 72°C.

    Purification:

    Article Title: Pgc-1? Overexpression Downregulates Pitx3 and Increases Susceptibility to MPTP Toxicity Associated with Decreased Bdnf
    Article Snippet: Expression Analysis After dissection as before, RNA was extracted from the striata or SN using the RNeasy mini kit (Qiagen). .. After DNase treatment the RNA was purified using the RNeasy MinElute Clean-up kit (Qiagen) and used as a template for cDNA synthesis with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Article Title: Transcriptome Pyrosequencing of the Parasitoid Wasp Cotesia vestalis: Genes Involved in the Antennal Odorant-Sensory System
    Article Snippet: RNA Preparation, cDNA Library Construction and 454 Sequencing Total RNA from 6 unit samples (a total of 360 female antennae) was isolated using a Qiagen RNeasy Mini Kit and an RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Total RNA (4.5 µg) was then taken through mRNA purification using a RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions (yielding 600 ng mRNA).

    Article Title: Genetic PEGylation
    Article Snippet: .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. PEG16–, PEG24–, and PEG48–AF–tRNA were purified by reversed-phase HPLC (Applied Biosystems POROS R2/10; 10 µm, 4.6 mm×100 mm) at a flow rate of 1.0 mL/min, with a linear gradient of 0%–100% acetonitrile in 0.1% triethylammonium acetate for 20 min.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Reduced Coupling of Oxidative Phosphorylation In Vivo Precedes Electron Transport Chain Defects Due to Mild Oxidative Stress in Mice
    Article Snippet: .. RT-PCR RNA was extracted from liquid nitrogen pulverized gastrocnemius using the RNeasy Mini Kit (Qiagen, Valencia, CA) and cDNA libraries were created using the Omniscript RT Kit (Qiagen). ..

    Article Title: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
    Article Snippet: Immediately after collection, the midguts were submersed into 3 ml of Buffer RLT (RNeasy Mini Kit, Qiagen, Valencia, CA, USA) supplemented with 30 μl of 2-mercaptoethanol, and thoroughly homogenized by passing the tissue mass through a 20-gauge needle (NN-2038R, Terumo, Tokyo, Japan) fitted to a 5 ml syringe (SS-05LZ, Terumo) five times. .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) using SYBR Green I dye (LightCycler FastStart DNA Master SYBR Green I, Roche Diagnostics, Mannheim, Germany) and the primer sets listed in Table was conducted to measure mRNA expression levels, as described previously [ ].

    Article Title: Protein Secretion Is Required for Pregnancy-Associated Plasma Protein-A to Promote Lung Cancer Growth In Vivo
    Article Snippet: Expression analysis of cancer cell lines by real-time PCR The RNeasy Mini kit (Qiagen, Valencia, CA) was used to prepare total RNA from cell culture. .. Synthesis of cDNA was set up using TaqMan RT-PCR reagents from Applied Biosystems (Life Technologies, Grand Island, NY).

    Article Title: Requirement for Zebrafish Ataxin-7 in Differentiation of Photoreceptors and Cerebellar Neurons
    Article Snippet: Paragraph title: RT-PCR Analysis of MOzatxnSPL Splice-blocking Activity ... RNAs were isolated using the RNeasy mini Kit (Qiagen, Germantown, MD, USA) and then reverse transcribed as cDNA using the SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.

    Article Title: P2X7 Receptor Activation Impairs Exogenous MHC Class I Oligopeptides Presentation in Antigen Presenting Cells
    Article Snippet: Paragraph title: Quantitative Reverse Transcriptase–polymerase Chain Reaction (RT-PCR) Analysis ... After LPS stimulation, total RNA was extracted using the RNeasy Mini kit (Qiagen), followed by reverse transcription using iScript cDNA Synthesis (Bio-Rad) with oligo(dT).

    Article Title: Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis
    Article Snippet: .. Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. .. In the case of terminally differentiated neuronal cultures, RNA extractions were performed at 59 days in vitro . cDNA was synthesized from 2 μg of RNA using Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and oligo-dT primers.

    cDNA Library Assay:

    Article Title: Transcriptome Pyrosequencing of the Parasitoid Wasp Cotesia vestalis: Genes Involved in the Antennal Odorant-Sensory System
    Article Snippet: .. RNA Preparation, cDNA Library Construction and 454 Sequencing Total RNA from 6 unit samples (a total of 360 female antennae) was isolated using a Qiagen RNeasy Mini Kit and an RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. Total RNA (4.5 µg) was then taken through mRNA purification using a RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions (yielding 600 ng mRNA).

    Software:

    Article Title: Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
    Article Snippet: Quantitative Real-Time PCR (qPCR) Total amount of RNA was extracted using RNeasy Mini Kit (Qiagen AG, Hombrechtikon, Switzerland) and the reverse transcription reaction was performed with 0.5 µg RNA in a 20 µl reaction using random primers (Invitrogen) with the Superscript III Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions. .. Primers were designed using the OLIGO 6.0 software (Molecular Biology Insights, Inc., Cascade, USA) (sequences are listed in ).

    Real-time Polymerase Chain Reaction:

    Article Title: Cohesin Is Required for Activation of MYC by Estradiol
    Article Snippet: RNA was processed using the Qiagen RNeasy mini kit with DNAse treatment (Qiagen). cDNA was transcribed using the Superscript III first strand synthesis system, according to the manufacturer’s instructions (Invitrogen). .. Real-time quantitative PCR was performed using Platinum® SYBR® Green qPCR SuperMix-UDG with ROX (Invitrogen) on an ABI 7300 Real Time PCR System (Applied Biosystems). qBase Plus (Biogazelle) was used to quantify the transcript levels of each gene, relative to endogenous controls for each sample.

    Article Title: Regulation of the Formyl Peptide Receptor 1 (FPR1) Gene in Primary Human Macrophages
    Article Snippet: .. Quantitative Real-Time PCR (qPCR) Total amount of RNA was extracted using RNeasy Mini Kit (Qiagen AG, Hombrechtikon, Switzerland) and the reverse transcription reaction was performed with 0.5 µg RNA in a 20 µl reaction using random primers (Invitrogen) with the Superscript III Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions. .. The qPCR reaction was done on a LightCycler 480 system (Roche Diagnostics, Rotkreuz, Switzerland) utilizing the hot-start SYBR green method with the following parameters: preheating for 10 min at 95°C, followed by 45 cycles of denaturation for 5 sec at 95°C, annealing for 10 sec at 60°C and extension for 6 sec at 72°C.

    Article Title: Protein Secretion Is Required for Pregnancy-Associated Plasma Protein-A to Promote Lung Cancer Growth In Vivo
    Article Snippet: .. Expression analysis of cancer cell lines by real-time PCR The RNeasy Mini kit (Qiagen, Valencia, CA) was used to prepare total RNA from cell culture. .. Total RNA was treated with DNase according to the manufacturer's instructions (Qiagen).

    Article Title: P2X7 Receptor Activation Impairs Exogenous MHC Class I Oligopeptides Presentation in Antigen Presenting Cells
    Article Snippet: After LPS stimulation, total RNA was extracted using the RNeasy Mini kit (Qiagen), followed by reverse transcription using iScript cDNA Synthesis (Bio-Rad) with oligo(dT). .. The mix SYBR Premix ExTaq (Takara) was used for quantitative PCR in iCycler MyiQ thermocycler (Bio-Rad).

    Article Title: The Dermatan Sulfate Proteoglycan Decorin Modulates ?2?1 Integrin and the Vimentin Intermediate Filament System during Collagen Synthesis
    Article Snippet: Paragraph title: mRNA Extraction and Quantitative Real-time PCR ... Total RNA was extracted from cells at day 3, 6 and 14 using RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 2 µg of total RNA using Omniscript RT Kit (Qiagen). mRNA levels of target genes were quantified by Real-Time RT-PCR using an ABI PRISM 7500 Sequence Detector (Applied Biosystems) (core unit IZKF Münster) and MESA GREEN (Eurogentec, Köln, Germany).

    Agarose Gel Electrophoresis:

    Article Title: Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis
    Article Snippet: Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. .. Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions.

    In Vitro:

    Article Title: Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis
    Article Snippet: Reverse transcription-polymerase chain reaction ( semi quantitative ) Total RNA was extracted from dissociated and washed cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. .. In the case of terminally differentiated neuronal cultures, RNA extractions were performed at 59 days in vitro . cDNA was synthesized from 2 μg of RNA using Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and oligo-dT primers.

    Spectrophotometry:

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop Technologies, Wilmington, DE). .. Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA.

    Concentration Assay:

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop Technologies, Wilmington, DE). .. Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA.

    Article Title: Genetic PEGylation
    Article Snippet: .. After incubation at 4°C for 2 h, potassium acetate (pH 4.5) was added to a final concentration of 0.3 M. PEG4– and PEG12–AF–tRNA were purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. PEG16–, PEG24–, and PEG48–AF–tRNA were purified by reversed-phase HPLC (Applied Biosystems POROS R2/10; 10 µm, 4.6 mm×100 mm) at a flow rate of 1.0 mL/min, with a linear gradient of 0%–100% acetonitrile in 0.1% triethylammonium acetate for 20 min.

    Lysis:

    Article Title: Effects of Fertility on Gene Expression and Function of the Bovine Endometrium
    Article Snippet: Microarray Analysis Total cellular RNA was isolated from frozen endometrium using Isol-RNA lysis reagent (5 Prime, Gaithersburg, MD) according to manufacturer's instructions and stored at −80°C in nuclease-free water. .. Isolated endometrial total RNA was treated with DNase I (Qiagen, Valencia, CA) and processed with RNeasy Mini Kit (Qiagen) according to manufacturer's instructions to remove genomic DNA.

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    Qiagen rnaeasy mini kit
    Rnaeasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 9314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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