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Merck KGaA ripa buffer
Ripa Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ripa buffer/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ripa buffer - by Bioz Stars, 2021-04
86/100 stars

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Related Articles

Western Blot:

Article Title: Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells
Article Snippet: Images were acquired using a Zeiss LSM 700 confocal microscope. .. Western blot Samples were lysed in ice-cold RIPA buffer and 10 μg of whole-cell lysate was resolved with SDS-PAGE gel then transferred to polyvinylidine difluoride membranes (Merck Millipore). .. Membranes were blocked with 5% milk in Tris-buffered saline and 0.05% TWEEN 20 (TBS-T, Sigma) for 1 h at room temperature, followed by primary antibody overnight at 4°C.

Article Title: CaMKII is involved in subcellular Ca2+ redistribution-induced endoplasmic reticulum stress leading to apoptosis in primary cultures of rat proximal tubular cells exposed to lead
Article Snippet: 488-nm laser was used to excite Mag-Fluo-4 fluorescence and [Ca2+ ]ER was calculated by the fluorescence intensity (FL-1, 530 nm) of 10,000 cells on flow cytometer. .. Western blot analysis Cells subjected to desired treatments were lysed in ice-cold RIPA buffer supplemented with protease inhibitor (Merck Millipore) and phosphatase inhibitor (Roche) to prepare the total cell lysates. .. After protein quantification with BCA method, samples were subjected to SDS-PAGE gels and transferred to PVDF membranes.

Article Title: Inhibition of FAK Signaling Elicits Lamin A/C-Associated Nuclear Deformity and Cellular Senescence
Article Snippet: The cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere at 5% CO2 , and treated with PF-573228 (TOCRIS, Bristol, UK) at concentrations of 0, 0.1, 1, or 10 μM. .. Western Blot Analysis The cells were harvested and lysed in 1x RIPA buffer (Merck, Darmstadt, Germany) containing protease and phosphatase inhibitors. .. The protein concentration was determined using a Bio-Rad DC protein assay kit (Bio-Rad, California, USA).

Article Title: Two transgenic mouse models for β-subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations
Article Snippet: Fluorescence was recorded in a Tecan Infinite® 200 PRO series plate reader using 495 and 585 nm excitation and emission wavelengths, respectively. .. Western blot analysis Isolated mitochondria were solubilized in RIPA buffer containing a cocktail of protease inhibitors (Protease Inhibitor Cocktail Set I, Merck Millipore, Billerica, MA, USA) and frozen at −80°C for further analysis. .. Frozen pellets were thawed on ice, and their protein concentration was determined using the bicinchoninic acid assay as detailed above, loaded at a concentration of 3.75 µg per well on the gels and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE).

Article Title: Magnesium lithospermate B protects the endothelium from inflammation-induced dysfunction through activation of Nrf2 pathway
Article Snippet: The following PCR primer pairs were used: ICAM1, 5′-GTG CAA GAA GAT AGC CAA CCA A-3′ (sense) and 5′-TGC CAG TTC CAC CCG TTC-3′ (antisense); VCAM1, 5′-TAC ATA TCA CCC AAG AAT ACAG-3′ (sense) and 5′-TTC CCA ATC AAA TTA ACT CC-3′ (antisense); TNFɑ, 5′-TCA ACC TCC TCT CTG CCA TC-3′ (sense) and 5′-CCA AAG TAG ACC TGC CCA GA-3′ (antisense); GAPDH, 5′-AAG AAG GTG GTG AAG CAG G-3′ (sense), and 5′-AGG TGG AGG AGT GGG TGT CG-3′ (antisense). .. Western blot analysisTotal protein from HMEC-1 cells was extracted by homogenization in ice-cold RIPA buffer supplemented with a protease inhibitor cocktail (Merck, Argentina; USA). .. Lung and aortic tissues were washed three times with ice-cold phosphate-buffered saline (PBS) to remove residual blood and then homogenized and lysed in ice-cold RIPA buffer.

SDS Page:

Article Title: Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells
Article Snippet: Images were acquired using a Zeiss LSM 700 confocal microscope. .. Western blot Samples were lysed in ice-cold RIPA buffer and 10 μg of whole-cell lysate was resolved with SDS-PAGE gel then transferred to polyvinylidine difluoride membranes (Merck Millipore). .. Membranes were blocked with 5% milk in Tris-buffered saline and 0.05% TWEEN 20 (TBS-T, Sigma) for 1 h at room temperature, followed by primary antibody overnight at 4°C.

Protease Inhibitor:

Article Title: CaMKII is involved in subcellular Ca2+ redistribution-induced endoplasmic reticulum stress leading to apoptosis in primary cultures of rat proximal tubular cells exposed to lead
Article Snippet: 488-nm laser was used to excite Mag-Fluo-4 fluorescence and [Ca2+ ]ER was calculated by the fluorescence intensity (FL-1, 530 nm) of 10,000 cells on flow cytometer. .. Western blot analysis Cells subjected to desired treatments were lysed in ice-cold RIPA buffer supplemented with protease inhibitor (Merck Millipore) and phosphatase inhibitor (Roche) to prepare the total cell lysates. .. After protein quantification with BCA method, samples were subjected to SDS-PAGE gels and transferred to PVDF membranes.

Article Title: Assessment of Anti-TNF-α Activities in Keratinocytes Expressing Inducible TNF- α: A Novel Tool for Anti-TNF-α Drug Screening
Article Snippet: .. The supernatant was removed and the remaining membrane fraction pellet resuspended in RIPA buffer (Merck Millipore, Billerica, USA) containing protease inhibitor cocktail (Roche, Mannheim, Germany). ..

Article Title: Two transgenic mouse models for β-subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations
Article Snippet: Fluorescence was recorded in a Tecan Infinite® 200 PRO series plate reader using 495 and 585 nm excitation and emission wavelengths, respectively. .. Western blot analysis Isolated mitochondria were solubilized in RIPA buffer containing a cocktail of protease inhibitors (Protease Inhibitor Cocktail Set I, Merck Millipore, Billerica, MA, USA) and frozen at −80°C for further analysis. .. Frozen pellets were thawed on ice, and their protein concentration was determined using the bicinchoninic acid assay as detailed above, loaded at a concentration of 3.75 µg per well on the gels and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE).

Article Title: Magnesium lithospermate B protects the endothelium from inflammation-induced dysfunction through activation of Nrf2 pathway
Article Snippet: The following PCR primer pairs were used: ICAM1, 5′-GTG CAA GAA GAT AGC CAA CCA A-3′ (sense) and 5′-TGC CAG TTC CAC CCG TTC-3′ (antisense); VCAM1, 5′-TAC ATA TCA CCC AAG AAT ACAG-3′ (sense) and 5′-TTC CCA ATC AAA TTA ACT CC-3′ (antisense); TNFɑ, 5′-TCA ACC TCC TCT CTG CCA TC-3′ (sense) and 5′-CCA AAG TAG ACC TGC CCA GA-3′ (antisense); GAPDH, 5′-AAG AAG GTG GTG AAG CAG G-3′ (sense), and 5′-AGG TGG AGG AGT GGG TGT CG-3′ (antisense). .. Western blot analysisTotal protein from HMEC-1 cells was extracted by homogenization in ice-cold RIPA buffer supplemented with a protease inhibitor cocktail (Merck, Argentina; USA). .. Lung and aortic tissues were washed three times with ice-cold phosphate-buffered saline (PBS) to remove residual blood and then homogenized and lysed in ice-cold RIPA buffer.

Isolation:

Article Title: Two transgenic mouse models for β-subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations
Article Snippet: Fluorescence was recorded in a Tecan Infinite® 200 PRO series plate reader using 495 and 585 nm excitation and emission wavelengths, respectively. .. Western blot analysis Isolated mitochondria were solubilized in RIPA buffer containing a cocktail of protease inhibitors (Protease Inhibitor Cocktail Set I, Merck Millipore, Billerica, MA, USA) and frozen at −80°C for further analysis. .. Frozen pellets were thawed on ice, and their protein concentration was determined using the bicinchoninic acid assay as detailed above, loaded at a concentration of 3.75 µg per well on the gels and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE).

Centrifugation:

Article Title: Downregulation of aquaporin 3 inhibits cellular proliferation, migration and invasion in the MDA-MB-231 breast cancer cell line
Article Snippet: .. For protein analysis, cells were lysed using RIPA buffer (Merck Millipore, Watford, UK) and insoluble material removed by centrifugation at 13,000 RCF, with the insoluble pellet being discarded. .. The protein content of the lysate was quantified using a modified Lowry method (DC Protein Assay; Bio-Rad Laboratories Ltd., Watford, UK) and photometrically measured at 690 nm.

Homogenization:

Article Title: Magnesium lithospermate B protects the endothelium from inflammation-induced dysfunction through activation of Nrf2 pathway
Article Snippet: The following PCR primer pairs were used: ICAM1, 5′-GTG CAA GAA GAT AGC CAA CCA A-3′ (sense) and 5′-TGC CAG TTC CAC CCG TTC-3′ (antisense); VCAM1, 5′-TAC ATA TCA CCC AAG AAT ACAG-3′ (sense) and 5′-TTC CCA ATC AAA TTA ACT CC-3′ (antisense); TNFɑ, 5′-TCA ACC TCC TCT CTG CCA TC-3′ (sense) and 5′-CCA AAG TAG ACC TGC CCA GA-3′ (antisense); GAPDH, 5′-AAG AAG GTG GTG AAG CAG G-3′ (sense), and 5′-AGG TGG AGG AGT GGG TGT CG-3′ (antisense). .. Western blot analysisTotal protein from HMEC-1 cells was extracted by homogenization in ice-cold RIPA buffer supplemented with a protease inhibitor cocktail (Merck, Argentina; USA). .. Lung and aortic tissues were washed three times with ice-cold phosphate-buffered saline (PBS) to remove residual blood and then homogenized and lysed in ice-cold RIPA buffer.

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    Merck KGaA radioimmunoprecipitation assay lysis buffer
    Radioimmunoprecipitation Assay Lysis Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/radioimmunoprecipitation assay lysis buffer/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    radioimmunoprecipitation assay lysis buffer - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA ripa based lysis buffer
    Ripa Based Lysis Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ripa based lysis buffer/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ripa based lysis buffer - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA ripa buffer
    Ripa Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ripa buffer/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ripa buffer - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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