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Merck & Co ripa buffer
Ripa Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ripa buffer/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ripa buffer - by Bioz Stars, 2021-04
86/100 stars

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Protease Inhibitor:

Article Title: LINC01088 inhibits tumorigenesis of ovarian epithelial cells by targeting miR-24-1-5p
Article Snippet: .. Cells were washed with phosphate-buffered saline (PBS) and lysed in 500 μl RIPA buffer with 5 μl protease inhibitor cocktail (100X, Merck, USA) on ice for 15 min, then centrifugation at 13 000 g for 10 min. .. The proteins in 20 μl supernatant were separated with 12% SDS-PAGE and transferred onto a nitrocellulose membrane.

Article Title: Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine
Article Snippet: .. Western Blot Total protein extracts from HeLa cells were obtained after lysis with cold RIPA buffer containing proteases inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, (Merck Madrid, Spain). .. Protein concentration was quantified using a BCA protein assay kit (Thermo Fisher Scientific, Sant Cugat del Vallés, Spain) [ ].

Article Title: Up‐regulation of CDHR5 expression promotes malignant phenotype of pancreatic ductal adenocarcinoma. Up‐regulation of CDHR5 expression promotes malignant phenotype of pancreatic ductal adenocarcinoma
Article Snippet: Both culture mediums were supplemented with 10% heat‐inactivated foetal bovine serum (SH30084.03, HyClone), and all the cells were cultured at 37°C under a humidified 5% CO2 atmosphere. .. 2.5 Western blotProteins were extracted from cells by RIPA buffer (1% Triton X‐100, 0.1% sodium dodecyl sulphate, 1% sodium deoxycholate, 0.15 m NaCl and 10 mm Tris, pH 7.2) with a protease inhibitor cocktail Set III (539134, Merck). ..

Article Title: Obesity reduces the anticancer effect of AdipoRon against orthotopic pancreatic cancer in diet-induced obese mice
Article Snippet: Cell viability was measured by trypan blue staining. .. Western blot analysis Panc02-Luc-ZsGreen cells were lysed in RIPA buffer containing Complete Protease Inhibitor Cocktail (Merck, Kenilworth, NJ, USA) and PhosSTOP (Merck). ..

Centrifugation:

Article Title: LINC01088 inhibits tumorigenesis of ovarian epithelial cells by targeting miR-24-1-5p
Article Snippet: .. Cells were washed with phosphate-buffered saline (PBS) and lysed in 500 μl RIPA buffer with 5 μl protease inhibitor cocktail (100X, Merck, USA) on ice for 15 min, then centrifugation at 13 000 g for 10 min. .. The proteins in 20 μl supernatant were separated with 12% SDS-PAGE and transferred onto a nitrocellulose membrane.

Western Blot:

Article Title: CuII(atsm) inhibits ferroptosis: Implications for treatment of neurodegenerative disease, et al. CuII(atsm) inhibits ferroptosis: Implications for treatment of neurodegenerative disease
Article Snippet: .. Samples were lysed in RIPA buffer (Merck) and proteins (15 μg) were resolved on NuPAGE 4‐12% Bis‐Tris gels and transferred to PVDF membranes with an iBlot2 for western immuno‐blotting (ThermoFisher Scientific). .. Primary antibodies used were rabbit anti‐GPX4 (1:5000, Abcam, Cambridge, USA) and mouse anti‐actin (1:10000, Merck).

Article Title: Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine
Article Snippet: .. Western Blot Total protein extracts from HeLa cells were obtained after lysis with cold RIPA buffer containing proteases inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, (Merck Madrid, Spain). .. Protein concentration was quantified using a BCA protein assay kit (Thermo Fisher Scientific, Sant Cugat del Vallés, Spain) [ ].

Article Title: Up‐regulation of CDHR5 expression promotes malignant phenotype of pancreatic ductal adenocarcinoma. Up‐regulation of CDHR5 expression promotes malignant phenotype of pancreatic ductal adenocarcinoma
Article Snippet: Both culture mediums were supplemented with 10% heat‐inactivated foetal bovine serum (SH30084.03, HyClone), and all the cells were cultured at 37°C under a humidified 5% CO2 atmosphere. .. 2.5 Western blotProteins were extracted from cells by RIPA buffer (1% Triton X‐100, 0.1% sodium dodecyl sulphate, 1% sodium deoxycholate, 0.15 m NaCl and 10 mm Tris, pH 7.2) with a protease inhibitor cocktail Set III (539134, Merck). ..

Article Title: Obesity reduces the anticancer effect of AdipoRon against orthotopic pancreatic cancer in diet-induced obese mice
Article Snippet: Cell viability was measured by trypan blue staining. .. Western blot analysis Panc02-Luc-ZsGreen cells were lysed in RIPA buffer containing Complete Protease Inhibitor Cocktail (Merck, Kenilworth, NJ, USA) and PhosSTOP (Merck). ..

Article Title: FGF signaling dynamics regulates epithelial patterning and morphogenesis
Article Snippet: 20,000 cells per well were seeded in polyHEMA-coated 6-well plates in mammosphere medium and cultured and quantified similarly as primary mammospheres. .. Western blot Organoid cultures were disintegrated by pipetting up and down in ice cold PBS with phosphatase inhibitors (10 mM β-glycerophosphate, 5 mM NaF, 1 mM Na3 VO4 ), spun down and lysed in RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) supplied with proteinase and phosphatase inhibitors (10 mM β-glycerophosphate, 5 mM NaF, 1 mM Na3VO4, 1 mM dithiotreitol, 0.5 mM phenylmethanesulphonylfluoride, 2 μg/ml aprotinin, 10 μg/ml leupeptin; all Merck). .. Protein lysates were homogenized by sonication, cleared by centrifugation and protein concentration was measured using the Bradford reagent.

Transfection:

Article Title: Widespread translational control regulates retinal development in mouse
Article Snippet: Western blots The translated peptides were further validated by western blot. .. Transfected N2A Cells were harvested with 100 μl of RIPA buffer (25 mM Tris•HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) added with 1X PIC (Merck, #539131) and Benzonase (NovoProtein, #M046-01B) and incubated 10 min on ice. ..

Incubation:

Article Title: Widespread translational control regulates retinal development in mouse
Article Snippet: Western blots The translated peptides were further validated by western blot. .. Transfected N2A Cells were harvested with 100 μl of RIPA buffer (25 mM Tris•HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) added with 1X PIC (Merck, #539131) and Benzonase (NovoProtein, #M046-01B) and incubated 10 min on ice. ..

Lysis:

Article Title: Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine
Article Snippet: .. Western Blot Total protein extracts from HeLa cells were obtained after lysis with cold RIPA buffer containing proteases inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, (Merck Madrid, Spain). .. Protein concentration was quantified using a BCA protein assay kit (Thermo Fisher Scientific, Sant Cugat del Vallés, Spain) [ ].

Mouse Assay:

Article Title: PNLDC1, mouse pre‐pi RNA Trimmer, is required for meiotic and post‐meiotic male germ cell development
Article Snippet: RT–qPCR was performed using the THUNDERBIRD™ SYBR qPCR Mix (TOYOBO) with the appropriate primers ( ) and the CFX384 Touch™ Real‐Time PCR Detection System (Bio‐Rad). .. Testes from E16.5 control and Pnldc1 mt/mt mice were homogenized by syringe in RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP40, 0.1% SDS, 0.25% sodium deoxycholate, 1 mM EDTA) and treated by Bezonase (Merck). .. Then, 0.5 testis lysate per lane was subjected to 5–20% SDS–PAGE.

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    Merck & Co ripa buffer
    Ripa Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ripa buffer/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ripa buffer - by Bioz Stars, 2021-04
    86/100 stars
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