ribopure yeast rna isolation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ribopure yeast rna isolation kit
    Ribopure Yeast Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure yeast rna isolation kit/product/Thermo Fisher
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ribopure yeast rna isolation kit - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Centrifugation:

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Dextrose-grown cells were harvested by centrifugation and then resuspended at a density of 2 × 106 cells/ml in prewarmed synthetic medium containing 50 mM of either dextrose or GlcNAc. .. RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP.

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: For comparisons of galactose alone with galactose plus GlcNAc, cells were grown overnight in a medium containing 2% galactose, harvested by centrifugation, and resuspended at 2 × 106 cells/ml in a synthetic medium containing 2% galactose; then GlcNAc was added to one sample to a final concentration of 5 mM. .. RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP.

    Amplification:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Synthesized:

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. Microarrays containing 60-mer oligonucleotides representing the open reading frames in the C. albicans ORF19 database were synthesized by Agilent Technologies (Santa Clara, CA).

    Article Title: The Sur7 Protein Regulates Plasma Membrane Organization and Prevents Intracellular Cell Wall Growth in Candida albicans
    Article Snippet: .. RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Austin, TX). cDNA was synthesized using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. After RNAse treatment, samples were purified on a silica gel column (Qiagen, Chatsworth, CA), coupled to NHS-reactive Cy3 or Cy5 dyes (GE Healthcare Bio-Sciences, Piscataway, NJ), and then dye incorporation was assayed spectrophotometrically.

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: .. RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP. .. Samples were treated with RNase A and were purified using a PCR cleanup column (Qiagen), and then the cDNA was coupled to N -hydroxysuccinimide (NHS)-functionalized Cy3 or Cy5 dyes (GE Biosciences).

    Article Title: N-Acetylglucosamine (GlcNAc) Induction of Hyphal Morphogenesis and Transcriptional Responses in Candida albicans Are Not Dependent on Its Metabolism *
    Article Snippet: RNA was extracted from a pellet of about 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). .. RNA samples were tested by PCR to confirm the absence of DNA contamination. cDNA was then synthesized from total RNA using an oligo(dT) primer (Invitrogen) and Superscript III reverse transcriptase (Invitrogen).

    Construct:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The construct was introduced into yeast strain YPH500 (ura3-52 lys2-801 amber ade2-101 ochre trp1-Δ63 his3-Δ200 leu2-Δ1 ) according to the pYES2 vector kit instructions (Invitrogen, Carlsbad, CA, USA). .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    SYBR Green Assay:

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination. .. The 10-μl reaction mixture included 2× iQ SYBR Green Supermix (Bio-Rad, Hercules, CA), 1 μl of first-strand cDNA reaction mixture, and 0.1 μM primers.

    Microarray:

    Article Title: Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
    Article Snippet: Paragraph title: Microarray analysis ... RNA from each pellet was isolated using the RiboPure-Yeast RNA isolation kit (Ambion, Inc.) as per the manufacturer’s instructions.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Paragraph title: Microarray analysis of gene expression ... RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP.

    Article Title: The Sur7 Protein Regulates Plasma Membrane Organization and Prevents Intracellular Cell Wall Growth in Candida albicans
    Article Snippet: Paragraph title: Microarray Analysis of Gene Expression ... RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Austin, TX). cDNA was synthesized using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP.

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: Paragraph title: Microarray analysis of gene expression. ... RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP.

    Incubation:

    Article Title: Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
    Article Snippet: The remaining culture was then pelleted, YPD + H2 O2 media removed, cells resuspended in 250 ml YPD, and culture incubated for 1 hr at 30°. .. RNA from each pellet was isolated using the RiboPure-Yeast RNA isolation kit (Ambion, Inc.) as per the manufacturer’s instructions.

    Article Title: N-Acetylglucosamine (GlcNAc) Induction of Hyphal Morphogenesis and Transcriptional Responses in Candida albicans Are Not Dependent on Its Metabolism *
    Article Snippet: Cells were grown overnight to log phase, washed, and then incubated in synthetic medium containing 50 m m dextrose or GlcNAc for 2 h, and the cell pellets were then frozen at −80 °C. .. RNA was extracted from a pellet of about 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion).

    Expressing:

    Article Title: The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching
    Article Snippet: Paragraph title: Analysis of SEC6 gene expression. ... Cells from an overnight culture were resuspended in fresh YPD with or without DOX and grown for 2 or 4 h. RNA was isolated using the RiboPure yeast RNA isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions.

    Article Title: Functional Analysis of the Exocyst Subunit Sec15 in Candida albicans
    Article Snippet: Paragraph title: Analysis of SEC15 gene expression. ... RNA was isolated using the RiboPure Yeast RNA Isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Paragraph title: Microarray analysis of gene expression ... RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP.

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Article Title: The Sur7 Protein Regulates Plasma Membrane Organization and Prevents Intracellular Cell Wall Growth in Candida albicans
    Article Snippet: Paragraph title: Microarray Analysis of Gene Expression ... RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Austin, TX). cDNA was synthesized using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Real-time qRT-PCR analysis of mRNA levels Cells were grown overnight in log phase and maintained at a low cell density to avoid high basal expression of the GlcNAc-induced genes in the h-d mutant, as described previously ( ). .. RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination.

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: Paragraph title: Microarray analysis of gene expression. ... RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching
    Article Snippet: Expression of SEC6 in the THE1-CIp10 control strain and tSEC6 after 2 and 4 h of growth in medium (with or without DOX) was assayed using reverse transcriptase PCR (RT-PCR) (see Fig. S1 in the supplemental material). .. Cells from an overnight culture were resuspended in fresh YPD with or without DOX and grown for 2 or 4 h. RNA was isolated using the RiboPure yeast RNA isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions.

    Article Title: Functional Analysis of the Exocyst Subunit Sec15 in Candida albicans
    Article Snippet: Expression of SEC15 in strains THE1-CIp10 and tetR-SEC15 was assayed using reverse transcriptase PCR (RT-PCR). .. RNA was isolated using the RiboPure Yeast RNA Isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions.

    Sequencing:

    Article Title: The Sur7 Protein Regulates Plasma Membrane Organization and Prevents Intracellular Cell Wall Growth in Candida albicans
    Article Snippet: RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Austin, TX). cDNA was synthesized using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. Control and experimental samples were mixed and hybridized in a Tecan (Durham, NC) HS4300 ProHybridization station to custom arrays from the Washington University Genome Sequencing Center that are spotted in triplicate with one 70-mer oligonucleotide for each open reading frame in the C. albicans ORF19 database ( ).

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP. .. Microarrays containing 70-mer oligonucleotides representing the open reading frames in the C. albicans ORF19 database spotted in triplicate were obtained from the Washington University Genome Sequencing Center ( ).

    RNA Sequencing Assay:

    Article Title: Eicosanoid biosynthesis influences the virulence of Candida parapsilosis
    Article Snippet: RNA extraction For RNA sequencing, the C. parapsilosis GA1 strain was grown overnight in 2 ml of YPD media at 30°C with continuous shaking applied at 180 rpm. .. After 3 hours of growth at 30°C, RNA was isolated using the Ribopure Yeast RNA isolation Kit (Ambion) following the manufacturer’s instructions.

    Mutagenesis:

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Real-time qRT-PCR analysis of mRNA levels Cells were grown overnight in log phase and maintained at a low cell density to avoid high basal expression of the GlcNAc-induced genes in the h-d mutant, as described previously ( ). .. RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination.

    Isolation:

    Article Title: The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching
    Article Snippet: .. Cells from an overnight culture were resuspended in fresh YPD with or without DOX and grown for 2 or 4 h. RNA was isolated using the RiboPure yeast RNA isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions. .. RT-PCR was performed using the Access RT-PCR system (Promega, Madison, WI) according to the manufacturer's protocol, using primers RT-SEC6-5Det and RT-SEC6-3Det ( ) and 1 μg total RNA as the template.

    Article Title: Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
    Article Snippet: .. RNA from each pellet was isolated using the RiboPure-Yeast RNA isolation kit (Ambion, Inc.) as per the manufacturer’s instructions. .. RNA probes were labeled and hybridized to Agilent Yeast 4x44K microarray kit by MoGene, LC (St. Louis, MO).

    Article Title: Eicosanoid biosynthesis influences the virulence of Candida parapsilosis
    Article Snippet: .. After 3 hours of growth at 30°C, RNA was isolated using the Ribopure Yeast RNA isolation Kit (Ambion) following the manufacturer’s instructions. .. For validating the RNA sequencing data, C. parapsilosis CLIB and GA1 cells were grown as described above and RNA extraction was performed using the same kit.

    Article Title: Functional Analysis of the Exocyst Subunit Sec15 in Candida albicans
    Article Snippet: .. RNA was isolated using the RiboPure Yeast RNA Isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions. .. RT-PCR was performed using the Access RT-PCR system (Promega, Madison, WI) according to the manufacturer's protocol, using primers RT-SEC15-5Det and RT-SEC15-3Det ( ) and 100 ng mRNA as the template.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: .. RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. After treatment with RNase A, the cDNA was purified using a PCR clean-up column (Qiagen, Valencia, CA) and then coupled to NHS-functionalized Cy3 or Cy5 dyes (GE Biosciences, Piscataway, NJ).

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: The Sur7 Protein Regulates Plasma Membrane Organization and Prevents Intracellular Cell Wall Growth in Candida albicans
    Article Snippet: .. RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Austin, TX). cDNA was synthesized using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. After RNAse treatment, samples were purified on a silica gel column (Qiagen, Chatsworth, CA), coupled to NHS-reactive Cy3 or Cy5 dyes (GE Healthcare Bio-Sciences, Piscataway, NJ), and then dye incorporation was assayed spectrophotometrically.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: .. RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination. .. The RNA was used as a template for cDNA synthesis using an oligo-dT primer (Invitrogen) and Superscript III reverse transcriptase (Invitrogen).

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: .. RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP. .. Samples were treated with RNase A and were purified using a PCR cleanup column (Qiagen), and then the cDNA was coupled to N -hydroxysuccinimide (NHS)-functionalized Cy3 or Cy5 dyes (GE Biosciences).

    Article Title: N-Acetylglucosamine (GlcNAc) Induction of Hyphal Morphogenesis and Transcriptional Responses in Candida albicans Are Not Dependent on Its Metabolism *
    Article Snippet: .. RNA was extracted from a pellet of about 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). .. RNA samples were tested by PCR to confirm the absence of DNA contamination. cDNA was then synthesized from total RNA using an oligo(dT) primer (Invitrogen) and Superscript III reverse transcriptase (Invitrogen).

    RNA Extraction:

    Article Title: Eicosanoid biosynthesis influences the virulence of Candida parapsilosis
    Article Snippet: Paragraph title: RNA extraction ... After 3 hours of growth at 30°C, RNA was isolated using the Ribopure Yeast RNA isolation Kit (Ambion) following the manufacturer’s instructions.

    Labeling:

    Article Title: Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
    Article Snippet: RNA from each pellet was isolated using the RiboPure-Yeast RNA isolation kit (Ambion, Inc.) as per the manufacturer’s instructions. .. RNA probes were labeled and hybridized to Agilent Yeast 4x44K microarray kit by MoGene, LC (St. Louis, MO).

    Purification:

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: .. RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. After treatment with RNase A, the cDNA was purified using a PCR clean-up column (Qiagen, Valencia, CA) and then coupled to NHS-functionalized Cy3 or Cy5 dyes (GE Biosciences, Piscataway, NJ).

    Article Title: The Sur7 Protein Regulates Plasma Membrane Organization and Prevents Intracellular Cell Wall Growth in Candida albicans
    Article Snippet: RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Austin, TX). cDNA was synthesized using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. After RNAse treatment, samples were purified on a silica gel column (Qiagen, Chatsworth, CA), coupled to NHS-reactive Cy3 or Cy5 dyes (GE Healthcare Bio-Sciences, Piscataway, NJ), and then dye incorporation was assayed spectrophotometrically.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination. .. The cDNA was separated from RNA by treatment with RNase A (New England Biolabs, Beverly, MA), purified using a PCR clean-up column (Qiagen), and then used as a template for qRT-PCR in an Eppendorf (Hauppauge, NY) Mastercycler EP Realplex2 .

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP. .. Samples were treated with RNase A and were purified using a PCR cleanup column (Qiagen), and then the cDNA was coupled to N -hydroxysuccinimide (NHS)-functionalized Cy3 or Cy5 dyes (GE Biosciences).

    Article Title: N-Acetylglucosamine (GlcNAc) Induction of Hyphal Morphogenesis and Transcriptional Responses in Candida albicans Are Not Dependent on Its Metabolism *
    Article Snippet: RNA was extracted from a pellet of about 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). .. The cDNA samples were treated with RNase A (New England Biolabs) and purified using a PCR cleanup column (Qiagen).

    Polymerase Chain Reaction:

    Article Title: The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching
    Article Snippet: Expression of SEC6 in the THE1-CIp10 control strain and tSEC6 after 2 and 4 h of growth in medium (with or without DOX) was assayed using reverse transcriptase PCR (RT-PCR) (see Fig. S1 in the supplemental material). .. Cells from an overnight culture were resuspended in fresh YPD with or without DOX and grown for 2 or 4 h. RNA was isolated using the RiboPure yeast RNA isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions.

    Article Title: Functional Analysis of the Exocyst Subunit Sec15 in Candida albicans
    Article Snippet: Expression of SEC15 in strains THE1-CIp10 and tetR-SEC15 was assayed using reverse transcriptase PCR (RT-PCR). .. RNA was isolated using the RiboPure Yeast RNA Isolation kit (Life Technologies, Grand Island, NY) according to the manufacturer's instructions.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP. .. After treatment with RNase A, the cDNA was purified using a PCR clean-up column (Qiagen, Valencia, CA) and then coupled to NHS-functionalized Cy3 or Cy5 dyes (GE Biosciences, Piscataway, NJ).

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: .. RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination. .. The RNA was used as a template for cDNA synthesis using an oligo-dT primer (Invitrogen) and Superscript III reverse transcriptase (Invitrogen).

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP. .. Samples were treated with RNase A and were purified using a PCR cleanup column (Qiagen), and then the cDNA was coupled to N -hydroxysuccinimide (NHS)-functionalized Cy3 or Cy5 dyes (GE Biosciences).

    Article Title: N-Acetylglucosamine (GlcNAc) Induction of Hyphal Morphogenesis and Transcriptional Responses in Candida albicans Are Not Dependent on Its Metabolism *
    Article Snippet: RNA was extracted from a pellet of about 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). .. RNA samples were tested by PCR to confirm the absence of DNA contamination. cDNA was then synthesized from total RNA using an oligo(dT) primer (Invitrogen) and Superscript III reverse transcriptase (Invitrogen).

    Quantitative RT-PCR:

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Paragraph title: Real-time qRT-PCR analysis of mRNA levels ... RNA was extracted from a pellet of ∼3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion) and was tested by PCR to confirm the absence of DNA contamination.

    Article Title: N-Acetylglucosamine (GlcNAc) Induction of Hyphal Morphogenesis and Transcriptional Responses in Candida albicans Are Not Dependent on Its Metabolism *
    Article Snippet: Paragraph title: Analysis of mRNA Levels Using Real Time Quantitative RT-PCR (qRT-PCR) ... RNA was extracted from a pellet of about 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion).

    cDNA Library Assay:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Plasmid Preparation:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The construct was introduced into yeast strain YPH500 (ura3-52 lys2-801 amber ade2-101 ochre trp1-Δ63 his3-Δ200 leu2-Δ1 ) according to the pYES2 vector kit instructions (Invitrogen, Carlsbad, CA, USA). .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Functional Assay:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: Paragraph title: Functional analysis of salt-responsive proteins in yeast ... Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Selection:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

    Concentration Assay:

    Article Title: Microarray Analysis of Gene Expression in Saccharomyces cerevisiae kap108Δ Mutants upon Addition of Oxidative Stress
    Article Snippet: Fresh H2 O2 was added to the remaining culture to a 1.0 mM final concentration, and each culture incubated for 10 min at 30°, after which 200 ml of culture was removed, spun, and frozen as above. .. RNA from each pellet was isolated using the RiboPure-Yeast RNA isolation kit (Ambion, Inc.) as per the manufacturer’s instructions.

    Article Title: Eicosanoid biosynthesis influences the virulence of Candida parapsilosis
    Article Snippet: Cell concentration was adjusted to 2 × 107 cells per 10 ml PBS supplemented with 500 μM of arachidonic acid in triplicates. .. After 3 hours of growth at 30°C, RNA was isolated using the Ribopure Yeast RNA isolation Kit (Ambion) following the manufacturer’s instructions.

    Article Title: Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression
    Article Snippet: Galactose-grown cells were resuspended at 2 × 106 cells/ml in 50 mM galactose, and then GlcNAc was added to one sample to a final concentration of 50 mM. .. RNA purified from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion, Grand Island, NY) was used as a template for cDNA synthesis using a Superscript II kit (Invitrogen) in a poly(T)-primed reaction containing a 3:2 mixture of aminoallyl-dUTP:dTTP plus dATP, dCTP, and dGTP.

    Article Title: Identification of GIG1, a GlcNAc-Induced Gene in Candida albicans Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ Needed for Normal Sensitivity to the Chitin Synthase Inhibitor Nikkomycin Z ▿ §
    Article Snippet: For comparisons of galactose alone with galactose plus GlcNAc, cells were grown overnight in a medium containing 2% galactose, harvested by centrifugation, and resuspended at 2 × 106 cells/ml in a synthetic medium containing 2% galactose; then GlcNAc was added to one sample to a final concentration of 5 mM. .. RNA was extracted from 3 × 108 cells using a RiboPure-Yeast RNA isolation kit (Ambion). cDNA was then synthesized from total RNA using a SuperScript II kit (Invitrogen) for a poly(T)-primed reaction with a mixture of aminoallyl-dUTP and dTTP in a 3:2 ratio plus dATP, dCTP, and dGTP.

    Marker:

    Article Title: Proteomic Analysis of Seedling Roots of Two Maize Inbred Lines That Differ Significantly in the Salt Stress Response
    Article Snippet: The ORFs were amplified by PCR from a maize cDNA library and cloned into the yeast expression vector pYES2, which contained the Ura3 selection marker. .. Yeast salt tolerance assays were performed according to Gao et al. [ ]; the NaCl concentrations were 0 M, 3 M, and 5 M. Yeast cells were collected before spotted on the agar plates, and yeast total RNAs were extracted using the RiboPure-Yeast RNA Isolation Kit (Life Technologies, Carlsbad, CA, USA).

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    Thermo Fisher ribopure yeast rna purification kit
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    Thermo Fisher ribopure yeast rna extraction kit
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