ribopure rna purification kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ribopure rna purification kit
    Ribopure Rna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure rna purification kit/product/Thermo Fisher
    Average 98 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    ribopure rna purification kit - by Bioz Stars, 2020-04
    98/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926). .. These cDNA samples were used as template for quantitative PCR experiments.

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific). .. Fast Start Universal SYBR Green Master (Rox) was used for PCR reactions (7 μL ddH2 O, 1 μL cDNA, 1 μL forward primer, 1 μL reverse primer, and 10 μL SYBR qPCR Mix).

    RNA Extraction:

    Article Title: Coxiella burnetii RpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth
    Article Snippet: Paragraph title: RNA extraction. ... RNA was subsequently purified using a Ribopure RNA purification kit (Thermo Fisher).

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: .. Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926). .. Purified RNA samples were reverse transcribed to single-stranded cDNA using High Capacity RNA-to-cDNA Kit (ThermoFisher, catalog number 4387406).

    Sequencing:

    Article Title: Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre
    Article Snippet: V3-V4 hypervariable region tag sequences were obtained using Illumina MiSeq sequencing of samples using a dual PCR approach [ ] at the DNA Service Facility at the University of Chicago, Illinois. .. RNA was extracted using the Ambion RiboPure RNA purification kit (Life Technologies, Grand Island, NY, USA) with the addition of a bead-beating step during TRI Reagent extraction as described in Shilova et al. [ ].

    Modification:

    Article Title: Coxiella burnetii RpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth
    Article Snippet: At 5 and 14 days of incubation, RNA was extracted using a modified hot TRIzol extraction. .. RNA was subsequently purified using a Ribopure RNA purification kit (Thermo Fisher).

    Article Title: Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre
    Article Snippet: DNA was extracted from filters using a modified Qiagen DNeasy Plant Kit (Valencia, California, USA) protocol as described in [ ]. .. RNA was extracted using the Ambion RiboPure RNA purification kit (Life Technologies, Grand Island, NY, USA) with the addition of a bead-beating step during TRI Reagent extraction as described in Shilova et al. [ ].

    Concentration Assay:

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: .. Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926). .. Purified RNA samples were reverse transcribed to single-stranded cDNA using High Capacity RNA-to-cDNA Kit (ThermoFisher, catalog number 4387406).

    Article Title: Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element
    Article Snippet: RNA isolation and cDNA synthesis We used a RiboPure RNA purification kit (Invitrogen) to purify RNA from actively dividing cultures. .. Following isolation, the concentration of RNA was determined with a Nanodrop 2000 spectrophotometer.

    Mutagenesis:

    Article Title: Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element
    Article Snippet: RNA isolation and cDNA synthesis We used a RiboPure RNA purification kit (Invitrogen) to purify RNA from actively dividing cultures. .. RNAs from WT and mutant strains were diluted to the same final concentration, and samples were frozen at -80°.

    Isolation:

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: .. Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific). .. Fast Start Universal SYBR Green Master (Rox) was used for PCR reactions (7 μL ddH2 O, 1 μL cDNA, 1 μL forward primer, 1 μL reverse primer, and 10 μL SYBR qPCR Mix).

    Article Title: Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element
    Article Snippet: .. RNA isolation and cDNA synthesis We used a RiboPure RNA purification kit (Invitrogen) to purify RNA from actively dividing cultures. ..

    Quantitative RT-PCR:

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: Paragraph title: Quantitative RT-qPCR experiments ... Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926).

    Purification:

    Article Title: Coxiella burnetii RpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth
    Article Snippet: .. RNA was subsequently purified using a Ribopure RNA purification kit (Thermo Fisher). ..

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: .. Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926). .. Purified RNA samples were reverse transcribed to single-stranded cDNA using High Capacity RNA-to-cDNA Kit (ThermoFisher, catalog number 4387406).

    Article Title: Extracellular Matrix in Cardiovascular Pathophysiology: Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents
    Article Snippet: .. LV tissue samples (50 mg) were homogenized in 1 ml of TRI reagent (AM9738, ThermoFisher Scientific), and RNA was extracted and purified from the tissue homogenates with the RiboPure RNA Purification Kit (AM1924, ThermoFisher Scientific) for storage at −20°C. .. RNA (1 μg) was converted into first strands with the RT2 First Strand Kit (330404, Qiagen), and the expression of 83 genes associated with cell adhesion and ECM molecules was quantified using the Rat ECM and Adhesion Molecules RT2 Profiler PCR Array (PARN-013Z, SuperArray Bioscience, Frederick, MD) according to the manufacturer's instructions.

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: .. Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific). .. Fast Start Universal SYBR Green Master (Rox) was used for PCR reactions (7 μL ddH2 O, 1 μL cDNA, 1 μL forward primer, 1 μL reverse primer, and 10 μL SYBR qPCR Mix).

    Article Title: Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre
    Article Snippet: .. RNA was extracted using the Ambion RiboPure RNA purification kit (Life Technologies, Grand Island, NY, USA) with the addition of a bead-beating step during TRI Reagent extraction as described in Shilova et al. [ ]. .. DNA was digested using the RNase-Free DNase Kit (Qiagen) according to the manufacturer’s protocol, and RNA quality and quantity was evaluated using the Agilent BioAnalyzer RNA Nano Kit (Agilent Technologies, Santa Clara, CA, USA) and Qiagen Qubit.

    Article Title: Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element
    Article Snippet: .. RNA isolation and cDNA synthesis We used a RiboPure RNA purification kit (Invitrogen) to purify RNA from actively dividing cultures. ..

    SYBR Green Assay:

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926). .. These qPCR included SYBR Green PCR Master Mix (ThermoFisher, catalog number 4309155), and primers at 5 μ m concentrations.

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific). .. Fast Start Universal SYBR Green Master (Rox) was used for PCR reactions (7 μL ddH2 O, 1 μL cDNA, 1 μL forward primer, 1 μL reverse primer, and 10 μL SYBR qPCR Mix).

    Polymerase Chain Reaction:

    Article Title: A biosensor-based approach reveals links between efflux pump expression and cell cycle regulation in pleiotropic drug resistance of yeast
    Article Snippet: Cultures were then diluted to A 600 = 0.2 in fresh minimal media and grown for a further 4 h. Cells were then treated with drugs at the inducing concentration ( and f ) for 1 h. 2 OD-equivalents of cells were then harvested for whole cell RNA extraction and DNA removal using a RiboPure RNA Purification Kit (ThermoFisher, catalog number AM1926). .. These qPCR included SYBR Green PCR Master Mix (ThermoFisher, catalog number 4309155), and primers at 5 μ m concentrations.

    Article Title: Extracellular Matrix in Cardiovascular Pathophysiology: Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents
    Article Snippet: Paragraph title: PCR gene arrays. ... LV tissue samples (50 mg) were homogenized in 1 ml of TRI reagent (AM9738, ThermoFisher Scientific), and RNA was extracted and purified from the tissue homogenates with the RiboPure RNA Purification Kit (AM1924, ThermoFisher Scientific) for storage at −20°C.

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific). .. Fast Start Universal SYBR Green Master (Rox) was used for PCR reactions (7 μL ddH2 O, 1 μL cDNA, 1 μL forward primer, 1 μL reverse primer, and 10 μL SYBR qPCR Mix).

    Article Title: Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre
    Article Snippet: V3-V4 hypervariable region tag sequences were obtained using Illumina MiSeq sequencing of samples using a dual PCR approach [ ] at the DNA Service Facility at the University of Chicago, Illinois. .. RNA was extracted using the Ambion RiboPure RNA purification kit (Life Technologies, Grand Island, NY, USA) with the addition of a bead-beating step during TRI Reagent extraction as described in Shilova et al. [ ].

    Incubation:

    Article Title: Coxiella burnetii RpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth
    Article Snippet: At 5 and 14 days of incubation, RNA was extracted using a modified hot TRIzol extraction. .. RNA was subsequently purified using a Ribopure RNA purification kit (Thermo Fisher).

    Spectrophotometry:

    Article Title: Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element
    Article Snippet: RNA isolation and cDNA synthesis We used a RiboPure RNA purification kit (Invitrogen) to purify RNA from actively dividing cultures. .. Following isolation, the concentration of RNA was determined with a Nanodrop 2000 spectrophotometer.

    Expressing:

    Article Title: Extracellular Matrix in Cardiovascular Pathophysiology: Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents
    Article Snippet: LV tissue samples (50 mg) were homogenized in 1 ml of TRI reagent (AM9738, ThermoFisher Scientific), and RNA was extracted and purified from the tissue homogenates with the RiboPure RNA Purification Kit (AM1924, ThermoFisher Scientific) for storage at −20°C. .. RNA (1 μg) was converted into first strands with the RT2 First Strand Kit (330404, Qiagen), and the expression of 83 genes associated with cell adhesion and ECM molecules was quantified using the Rat ECM and Adhesion Molecules RT2 Profiler PCR Array (PARN-013Z, SuperArray Bioscience, Frederick, MD) according to the manufacturer's instructions.

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: Expression levels of nestin, microtubule-associated protein 2 (MAP2) (Hashemi et al., 2017), tafazzin (Zhou et al., 2016), and protein phosphatase 1 (PP1C) (Fang et al., 2018) were normalized to the expression level of β-actin (Brett et al., 2011) ( ). .. Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium
    Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) analysis ... Total RNA was isolated using a RiboPure™ RNA purification kit (AM1924; Thermo Fisher Scientific, Waltham, MA, USA), and 2.0 µg of total RNA was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (4368814, Thermo Fisher Scientific).

    Microarray:

    Article Title: Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre
    Article Snippet: RNA was extracted using the Ambion RiboPure RNA purification kit (Life Technologies, Grand Island, NY, USA) with the addition of a bead-beating step during TRI Reagent extraction as described in Shilova et al. [ ]. .. All samples with an RNA Integrity Number greater than 9 were processed for microarray analyses ( > 7 RIN was recommended by the microarray facility).

    Hybridization:

    Article Title: Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre
    Article Snippet: RNA was extracted using the Ambion RiboPure RNA purification kit (Life Technologies, Grand Island, NY, USA) with the addition of a bead-beating step during TRI Reagent extraction as described in Shilova et al. [ ]. .. Microarray hybridization was performed at the Roy J.

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  • News
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  • Partners
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  • Bioz Stars
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  • 99
    Thermo Fisher ribopure rna purification kit
    Ribopure Rna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure rna purification kit/product/Thermo Fisher
    Average 99 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    ribopure rna purification kit - by Bioz Stars, 2020-04
    99/100 stars
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